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Reposted by Shivani Dharmadhikari

preLights @prelights.bsky.social · 25/02/2026

How to make a 4-cell square ascidian embryo? Cytoplasmic pulling rotates centrosomes, cortical forces lock the plane. Corentin Mollier & Shivani Dharmadhikari highlight a #preprint from Silvia Caballero-Mancebo & team @rdumollard.bsky.social #preLight ⬇️ prelights.biologists.com/highlights/c...

prelights.biologists.com

Cooperation between cortical and cytoplasmic forces shapes planar 4-cell stage embryos - preLights

How to make a 4-cell square ascidian embryo? Cytoplasmic pulling rotates centrosomes, cortical forces lock the plane.

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 27/01/2026

It was only for the 24th and 25th actually (so over the last weekend)!

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 25/01/2026

It’s fills you with childlike wonder and delight to see what you see under the microscope all the time on these magnificent walls and to see it shared with everyone who doesn’t do science(as a profession)☺️ #actin#cytoseleton#cells#protrusions

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 25/01/2026

One the most lovely combinations of science and art I have been to in a long time at Musée d’Orsay☺️✨ by the @manuelthery.bsky.social #cytomorpholab

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Reposted by Shivani Dharmadhikari

Manuel Thery @manuelthery.bsky.social · 05/12/2025

#LivingArchitectures We put cells and cytoskeleton filaments on the architecture of the musée d'Orsay. www.musee-orsay.fr/fr/agenda/ev... Scientists of the #CytoMorphoLab adapted their protocols to illustrate the questions that keep them awake at night. -> Two shows on the 24th and 25th of January.

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 13/11/2025

A fun way to recover embryos on a grey Parisian day 🌧️🐭

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 08/08/2025

It was lovely to know you and everyone else from your lab too ☺️

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 07/08/2025

I am sure I am missing to tag so many of them here but hopefully my friends from the course will help me tag them:) with that being said MBL Physiology 2025 will be a lovely memory to cherish! ☺️

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 07/08/2025

With social hours spent doing foldscope duels @prakashlab.bsky.social @yannis-reignier.bsky.social and solving puzzles.. watching whales and late night swims under the stars 🌌🌊🤫 with my cohort @edwardwheeler.bsky.social @vibhaka.bsky.social @rnadavid.bsky.social @michaelcchung.bsky.social ....

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 07/08/2025

And the course manager - @samjlord.bsky.social 💪 and the lovely course assistants Peter Yunker, Pascal Keum-jae, Noah Kohn

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 07/08/2025

Throughout the summer we had the support of very talented microscopists - Joe Brzostowski, @tanner-fadero.bsky.social , Nico Stuurman! 🔬😊

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 07/08/2025

And we finished with collectives of collectives and electrocuting things ⚡ with @djcohen.bsky.social, Isaac Breinyn, Sameeksha Rao, Yubin Lin and Charlie Duclut

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 07/08/2025

Rotation 2 continued with amazing tools to look at organelles inside the cell with @cohenlaboratory.bsky.social, Sherry Hsu, Kajal Kamble, @mczanellati.bsky.social! Had a cool group to troubleshoot with Safal, Greg, Alex, Aaron, @edwardwheeler.bsky.social , Illeana and @lucasphilipp1.bsky.social

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 07/08/2025

Rotation 1 introduced me to the amazing Physarum 😍💛 Amy, Zach, Ameya, @haysconun.bsky.social and Vita from @gladfelterlab.bsky.social were encouraging TA's.

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 07/08/2025

Spent a memorable summer at Physiology course at @mblscience.bsky.social Very coooool science with some lovely people! Thanks @brangwynnelab.bsky.social and @gladfelterlab.bsky.social for being amazinnnng course coordinators! 🧵

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Reposted by Shivani Dharmadhikari

Tanner Fadero @tanner-fadero.bsky.social · 04/02/2025

I'll kick off my bsky profile with a great 3D fluorescence microscopy time-lapse. My buddies Joe Brzostowski and Rachel Stadler (NIH) acquired this on their #snoutscope that I helped them build. Shown is a fluorescent actin marker in Dictyostelium. Joe and Rachel called this "The Mark of Zorro!"

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Reposted by Shivani Dharmadhikari

Marine Biological Laboratory @mblscience.bsky.social · 25/06/2025

MBL scientists use skates and their embryos to study skeletal development. By shining a light through egg cases, scientists can watch the entire maturation process. Learn more about skates as a research organism here: bit.ly/3Rmvq3F 📹 Bioquest

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Reposted by Shivani Dharmadhikari

brangwynnelab.bsky.social @brangwynnelab.bsky.social · 18/06/2025

The one and only Manu @prakashlab.bsky.social inspiring @mblscience.bsky.social Physiology students at this morning's lecture.

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Reposted by Shivani Dharmadhikari

brangwynnelab.bsky.social @brangwynnelab.bsky.social · 17/06/2025

A great morning with the @mblscience.bsky.social Physiology students after my talk today.

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Reposted by Shivani Dharmadhikari

Erwin Frey @physicsoflifelmu.bsky.social · 31/03/2025

We may not have really squared the circle — but we came close! 😉 In our @NaturePhysics paper w/ @fakhrilab.bsky.social, light-triggered membrane excitability enables programmable shapes — a step toward engineering living matter. 🔗 www.nature.com/articles/s41... #biophysics #syntheticcell #softmatter

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 24/04/2025

Is there a pattern of how they arrange in the drop or have I been staring through the microscope for far toooo long? 🧐😁#beautifulembryos

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 22/03/2025

That’s beautiful! What did you use to stain the membrane?

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Reposted by Shivani Dharmadhikari

Viroscope @viroscope.bsky.social · 21/03/2025

New cell line = New cell morphologies to explore! This hepatic cell line has fully invested into membrane protrusions! Actin is shown in 🟦 and DNA in 🟧 #FluorescenceFriday #SciArt

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Reposted by Shivani Dharmadhikari

John Wallingford @jbwallingford.bsky.social · 11/03/2025

A woman studies embryos for decades, struggles for funding and respect. She publishes under her husband’s name, until they divorce. A single mother, she moves across an ocean to continue her studies. It all sounds quite modern, until you learn that she also worried she’d be burned as a witch. 🧵 1/15

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 09/02/2025

Lovely work on shape evolution! Check out the story below:)

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 07/02/2025

Couldn’t help but hum.. Round like a circle in a spiral, like a wheel within a wheel…🎶the windmills of your mind!

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 05/02/2025

As a biologist, we have to often choose colors and the way of representation of data..but Pierre-Joseph Redouté’s plant illustrations are amazing and Nicholas Rougeux's collation and post processing and representation of data is beautiful! Lots of amazing stuff! check here - www.c82.net/work/

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 06/01/2025

Thank you for sharing! Its super helpful and I couldn’t agree more with you:)

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 04/01/2025

Interesting topic and a lovely lab to work in! Don’t miss out on the opportunity!

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 27/12/2024

Such an important way of progressing in any scientific question, yet so undervalued!

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 18/12/2024

There is the beast of the year! Little hunting 🐙 And the beauty by Manon’s lovely heart! Its nice to be a part of the yearly tradition! 🎄

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 14/12/2024

Wow, that looks like it has tons of plasma membrane stores! How does it synchronise the filopodial formation?

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Reposted by Shivani Dharmadhikari

Thibaut Brunet @thibautbrunet.bsky.social · 07/12/2024

Yes! everybody has their favorite early Metchnikoff paper (before he moved on to such secondary pursuits as discovering innate immunity). Mine: his early intuition that animals evolved from colonies of flagellates that could transdifferentiate into amoebae - 100y before molecular phylogenies!

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 30/11/2024

An interesting read! An article from John Hopfield on how he chooses to work on the next question(now what?)… a question, as a scientist we often ask ourselves! pni.princeton.edu/people/john-...

pni.princeton.edu

Now What?

John J. Hopfield, Princeton University, Princeton, NJ 08544, USA. October 2018 John J. Hopfield Now What? (Article in PDF format) My first full-time permanent employment was at the Bell Telephone Labo...

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Reposted by Shivani Dharmadhikari

Michalis Averof @michalis-averof.bsky.social · 12/11/2024

Looking for a tag to localise fluorescent proteins to the plasma membrane? Unsure which tag might work in your species? We generated a toolkit of 11 membrane-localising tags, which can be screened rapidly by microinjecting mRNA in your species of interest. 1/4 www.biorxiv.org/content/10.1...

biorxiv.org

A toolkit for testing membrane localisation tags across species

Transgenic markers and tools have revolutionised how we study cells and developing organisms. Some of the elements needed to construct those tools are universally applicable (e.g. fluorescent proteins...

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Reposted by Shivani Dharmadhikari

Jean Léon Maître @maitrejl.bsky.social · 26/11/2024

Congrats to double Doctor Julie Firmin on receiving the prize for her Great Advances in Biology from the Academie des Sciences!

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 22/11/2024

The first apico-basal polarity is established at this stage in the embryo😁

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 22/11/2024

Early mouse morula! Almost two years ago, I imaged my very first mouse embryo and since then I haven’t gotten over the funny apical domains (cyan)😍 nostalgic :) This beauty was part of the first immunostaining, I did when I joined the lab! #fluoroscentfriday

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 21/11/2024

First snow of the year!! For someone who was used to 30 degrees all year along, snowing is special❄️🌨️ I will try to work today😉

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 19/11/2024

We should definitely get you those eye gear✌️😎

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 19/11/2024

And the image was taken by lazer scanning microscope, Zeiss LSM 900

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 19/11/2024

Sure, if it helps you! It was a really silly experiment_ in magenta and green (phalloidin staining marking actin, I ended up adding two phalloidin stains 🙈) and nuclei (dapi in cyan).

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 19/11/2024

The embryos usually have to hatch from the shell (zona pellucida) that surrounds them because as they grow, because of the growing lumen (blastocoel).

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Shivani Dharmadhikari @sdharmadhikari.bsky.social · 19/11/2024

Hatching blastocyst! When you fix the embryos wayyyy later than you wanted to, because you got caught up;) still a beautiful one though 😉☺️

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