Mechanical forces shape the developing heart by controlling cell volume through a Piezo1-driven hydraulic mechanism.

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Discover how zebrafish labs and other aquatic research facilities are leading the way towards greener science! Learn how labs are cutting water and energy use, reducing waste, and exploring sustainable...

The developing heart integrates several progenitor cell types. Here they show that the pericardium enveloping the heart develops among cells that form the mesothelium around inner organs and body cavities, distinct from the classic heart field.

Rosalind Franklin never lived to see the full impact of her discoveries. She didn’t witness how her work on DNA would revolutionise biology, nor did she witness the impact she would have in…

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Pyruvate is the end-product of glycolysis and a central metabolite involved in many biochemical pathways. However, a lack of high-performance (i.e., Δ F / F > 10) single fluorescent protein (FP)-base...

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The Melt (Membrane localization using temperature) protein translocates to the plasma membrane upon temperature shift. Melt variants with a range of switching temperatures enable straightforward therm...

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The structural development of the heart depends heavily on mechanical forces, and rhythmic contractions generate essential physical stimuli during mor…

Potassium ion (K+) is the most abundant metal ion in cells and plays an indispensable role in practically all biological systems. Although there have been reports of both synthetic and genetically encoded fluorescent K+ indicators, there remains a need for an indicator that is genetically targetable, has high specificity for K+ versus Na+, and has a high fluorescent response in the red to far-red wavelength range. Here, we introduce a series of chemigenetic K+ indicators, designated as the HaloKbp1 series, based on the bacterial K+-binding protein (Kbp) inserted into HaloTag7 self-labeled with environmentally sensitive rhodamine derivatives. This series of high-performance indicators features high brightness in the red to far-red region, large intensiometric fluorescence changes, and a range of Kd values. We demonstrate that they are suitable for the detection of physiologically relevant K+ concentration changes such as those that result from the Ca2+-dependent activation of the BK potassium channel.

Studying RNA dynamics in vivo often relies on fluorogenic approaches, but these can be hampered by factors such as limited sensitivity and sample autofluorescence. Here, the authors describe an ultras...

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