Hexokinase detachment from the outer mitochondrial membrane is shown to support aerobic glycolysis in cancer cells. Differential localization of the HK1 isoform to the outer mitochondrial membrane, co...

Human plasma-like medium (HPLM), a synthetic physiologic medium containing over 60 metabolites and small ions at concentrations reflective of adult human plasma, enhances the structural and metabolic....

The de novo purine synthesis pathway is fundamental for nucleotide production, yet the role of mitochondrial metabolism in modulating this process rem…

Cytotoxic chemotherapy remains the standard-of-care treatment for patients with pancreatic ductal adenocarcinoma (PDAC). However, chemotherapy only has modest effects at improving patient survival due...

Chen et al. show that PEX39 cooperates with PEX7 in the peroxisomal import of proteins containing a PTS2 site and uncover an (R/K)PWE motif in PEX39 and PEX13 that binds to PEX7 and facilitates the im...

Nature Chemistry - In living systems, ATP provides an energetic driving force for protein synthesis and modification. Now, an engineered enzymatic tool has been developed for high-yield, ATP-driven...

Hundreds of mitochondrial-destined proteins rely on N-terminal presequences for organellar targeting and import. While generally described as positively charged amphipathic helices, presequences lack ...

Nature Metabolism, Published online: 02 May 2025; doi:10.1038/s42255-025-01272-3In this study, Flickinger et al. uncover the essential role of cytosolic NADK and why the relative importance of this role further depends on folate availability.

ALT: a penguin is reading a book while sitting on a pile of books reading vibes

Maturing human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) in vitro is critical for advancing drug discovery and cardiotoxicity screening applications of these cells. However, the metaboli...

Background In vivo stable isotope tracing is useful for natively surveying glioma metabolism but can be difficult to implement. Stable isotope tracing is tractable using in vitro glioma models, but mo...

Pathway analysis, originally developed for gene expression data, has been adapted for metabolomics. However, owing to the unique characteristics and constraints of metabolites compared with genes, pat...

The metabolic environment experienced by cultured cells often differs from physiological conditions. Here, we highlight the effects that the microenvironment can have on cultured cell behavior and adv...

NIH’s abrupt decision to cap indirect cost reimbursement at 15% threatens the critical infrastructure supporting groundbreaking biomedical research in the United States. This policy jeopardizes Americ...

Move to cut indirect cost rate to 15% could cost universities billions of dollars

Move to cut indirect cost rate to 15% could cost universities billions of dollars