Based on JAX, Chromatix provides a standardized framework for developing, sharing and reusing wave-optics simulations. The capabilities are demonstrated in a number of computational microscopy applica...

We present a tunable microfabrication pipeline for creating robust, reflective inserts that adapt conventional commercial imaging chambers for single-objective light sheet (LS) illumination. This system reduces the complexity associated with dual-objective LS setups and specialized LS chambers while retaining the native functionality and biocompatibility of the original chambers. The fabricated insert features a metalized, 3D nanoprinted micromirror with an angled reflective surface, enabling alignment of a thin LS for sectioning and imaging throughout mammalian cells. Using this pipeline, we demonstrate that single-objective LS illumination achieves an over 4X improvement in the signal-to-background ratio compared with conventional widefield epi-illumination in both fixed and live cell samples. Furthermore, we show substantial resolution enhancement for single-molecule localization microscopy compared to epi-illumination for improved imaging at the nanoscale. The versatile and scalable design offers an easily implemented approach to bring the benefits of single-objective LS microscopy to a wide array of biological studies.

Reconciling the micro and the macro evidence

Accelerating science writing and collaboration with AI.

Landmark deal should raise questions about publisher’s practices on peer review, citations and special issues, say research sleuths

Manipulating the topology of electronic bands can realize new states of matter, with possible implications for information technology. A central question is how to tell whether a topological regime ha...

De Chinese exportmachine is een wake-upcall voor economische beleidsmakers in de hele wereld. Importbarrières vormen niet het juiste antwoord. Wat dan wel?

Franklin was no victim in how the DNA double helix was solved. An overlooked letter and an unpublished news article, both written in 1953, reveal that she was an equal player.

Artificial intelligence and parallel computing could refine studies of the movement of single molecules inside cells.

Nature Communications - The realization that the cell is abundantly compartmentalized into biomolecular condensates has opened new opportunities for understanding the physics and chemistry...

Motion models (i.e., transition probability densities) are often deduced from fluorescence widefield tracking experiments by analyzing single-particle trajectories post-processed from data. This analy...

Here are our current open positions: Editor, Medical Evidence Project Staff reporter, Retraction Watch Assistant researcher, Retraction Watch Database Learn more about the Center for Scientific Int…

A wide-field microscope capable of simultaneously measuring circular dichroism and circular birefringence signals over wide fields of view of the order of hundreds of micrometres is demonstrated, addr...

Research and innovation news alert: As part of the next long-term EU budget 2028-2034, the Commission is proposing to double the budget of the research and innovation framework programme to €175 billi...

Introducing a new careers interview series, featuring developmental biologists who have had vastly different career trajectories.