piatkevich.bsky.social @piatkevich.bsky.social · 04/06/2026We are carrying out large-scale validation and benchmarking of AI agents for multi-omics data analysis and would like your feedback on the usage of these tools in your research (click the link for the survey) forms.office.com/Pages/Respon... 000
piatkevich.bsky.social @piatkevich.bsky.social · 01/06/2026Having fun recording videos for my next lecture on Optogenetics in the Neurotechnology course at Westlake University. 000
piatkevich.bsky.social @piatkevich.bsky.social · 09/03/2026Genspark helps me develop engaging opening slides and format my presentations for the undergraduate neurotechnology course I teach this semester www.genspark.ai/invite_membe... 000
Reposted by @piatkevich.bsky.socialManuel Lera-Ramirez @manu-lera.bsky.social · 20/02/2026🚀 Excited to launch the #OpenCloning Assembler! 🧬 Built to demystify Golden Gate for beginners and save time for experts -> MoClo assembly blazing fast ⚡ ✍️ Use existing syntaxes or design your own! #SynBio #GoldenGate #MoClo #OpenSource #Biotech 👉 Try it: app.opencloning.org Feedback welcome! 43214
piatkevich.bsky.social @piatkevich.bsky.social · 23/12/2025Wow! This is so cool and way more fun than boring BioRender! Plus, developing fine motor skills through hand drawing and creativity without limitations in shapes or form! Try it out yourself here chat.figurelabs.ai/invitation/i... 021
piatkevich.bsky.social @piatkevich.bsky.social · 23/12/2025It was great pleasure hosting you at Westlake University! And you research is just mindblowing! I am looking forward to all cool technologies from your lab! 011
piatkevich.bsky.social @piatkevich.bsky.social · 16/12/2025Excited to share insights on iPEX, a new technology co-developed with Yilong Zou for micrometre-resolution spatial proteomics, published in @Nature. Check out this interview to discover how tissue expansion is revolutionizing protein analysis in intact tissue! www.youtube.com/watch?v=6hKn...youtube.comWestlake’s iPEX: When Proteins Can’t Hide AnymoreYouTube video by Westlake University 040
piatkevich.bsky.social @piatkevich.bsky.social · 25/11/2025Gearing up for next year’s teaching at Westlake University. Here’s my intro slide for Super-Resolution Imaging Techniques 020
piatkevich.bsky.social @piatkevich.bsky.social · 19/11/2025Next-generation orange-to-far-red photoconvertible fluorescent protein for single-molecule microscopy and protein dynamic tracking www.nature.com/articles/s41... 0110
piatkevich.bsky.social @piatkevich.bsky.social · 13/11/2025In situ imaging proteomics via expansion (iPEX) integrates isotropic tissue magnification with MALDI mass spectrometry imaging to enable unprecedented spatial resolution and depth of protein identification for untargeted spatial proteomics www.nature.com/articles/s41...nature.comiPEX enables micrometre-resolution deep spatial proteomics via tissue expansion - NatureIsotropic tissue magnification is integrated with matrix-assisted laser desorption/ionization mass spectrometry imaging to enable untargeted spatial proteomics at micrometre resolution and with high p... 010
piatkevich.bsky.social @piatkevich.bsky.social · 12/11/2025Our red calcium indicator, FRCaMPi, and its soma-localized version, SomaFRCaMPi, are now available from @addgene.bsky.social in various vectors, along with all the sensors used for benchmarking. www.addgene.org/browse/artic... 0237
piatkevich.bsky.social @piatkevich.bsky.social · 11/11/2025ProteomEx is perhaps the most straightforward and simple approach for spatially resolved targeted proteomics, which requires only off-the-shelf reagents. Now with this step-by-step protocol published in Nature Protocols, anyone can do it www.nature.com/articles/s41...nature.comFilter-aided expansion proteomics for the spatial analysis of single cells and organelles in FFPE tissue samples - Nature ProtocolsFilter-aided expansion proteomics facilitates spatial proteomics at subcellular resolution by integrating tissue expansion, imaging-guided microdissection and filter-aided in-gel digestion. This combi... 000
piatkevich.bsky.social @piatkevich.bsky.social · 06/11/2025AAVs are crucial vectors for delivering biosensors into neurons! Using AAV delivery, we perform two-color imaging of calcium and potassium transients in neurons. These AAVs are now available from @braincase.bsky.social www.ebraincase.com/news/new-pro... 2238
piatkevich.bsky.social @piatkevich.bsky.social · 30/10/2025🔬 FULL WEBINAR NOW AVAILABLE! Spatial Biology: Innovations & Applications Featuring experts: → Xiao Wang → Kiryl D. Piatkevich → Jeffrey Spraggins → Fei Chen Watch the complete session to discover cutting-edge tech 👇 live.vhall.com/v3/lives/wat... 051
piatkevich.bsky.social @piatkevich.bsky.social · 30/10/2025What an incredible experience hosting a webinar on Spatial Biology: Innovations & Applications with @Science/AAAS! Missed it? Don't worry—we're dropping the full recording soon! Here's a sneak peek 👇 010
piatkevich.bsky.social @piatkevich.bsky.social · 27/10/2025Great commentary by @yulonglilab on our two recent papers introducing red genetically encoded biosensors for calcium and potassium imaging! 🔴 FRCaMPi - Enhanced red calcium sensor 🔴 RGEPOs - First-ever red potassium sensors 📖 doi.org/10.1371/jour...doi.orgIlluminating calcium and potassium dynamics with red fluorescent sensorsDespite recent advances in green genetically encoded ion indicators, developments in red-shifted indicators have been slower. This Primer explores two recent studies in PLOS Biology that engineer red ... 052
piatkevich.bsky.social @piatkevich.bsky.social · 24/10/2025Spatial biology technologies are rapidly advancing, delivering unprecedented insights into cellular organization and disease pathology. Join our October 29th webinar on "Spatial Biology: Innovations & Applications" with great speakers. Register here for free en.westlake.edu.cn/academics/Sc... 020
piatkevich.bsky.social @piatkevich.bsky.social · 23/10/2025High-quality AAVs for red genetically encoded calcium indicator FRCaMPi are now available from BrainCase anywhere in the world www.ebraincase.com/products-ser... 020
piatkevich.bsky.social @piatkevich.bsky.social · 22/09/2025#OpenCloning opencloning.org now features WeKwikGene integration—streamlining downloading plasmid sequences from wekwikgene.wllsb.edu.cn and downstream cloning and collaborations! 021
piatkevich.bsky.social @piatkevich.bsky.social · 16/09/2025Fluorescent protein-based ticker tapes (FPTT) record transcriptional histories of multiple promoters on single cellular fibers. Quantitative readout is available at any time point. Cells become biological archives of their own gene expression biorxiv.org/content/10.1... 030
piatkevich.bsky.social @piatkevich.bsky.social · 15/09/2025Major milestone for WeKwikGene! wekwikgene.wllsb.edu.cn now features 1,000+ ready-to-ship plasmids with 800+ more plasmids under QC. We've processed 1,000+ requests & distributed 3,000+ plasmids worldwide. 011
piatkevich.bsky.social @piatkevich.bsky.social · 10/09/2025Our blue FP Electra2 shows excellent brightness & localization in soil-dwelling amoeba D. discoideum as fusion marker, with performance comparable to the golden standard mTagBFP2, all plasmids for Electra2 are available from WeKwikGene wekwikgene.wllsb.edu.cn/publications... 110
piatkevich.bsky.social @piatkevich.bsky.social · 09/09/2025The hCRAFi-CCR2 sensor reports chemokine release in the mouse visual cortex after LPS challenge using two-photon microscopy www.nature.com/articles/s41.... Plasmids and corresponding test AAVs can be requested from WeKwikGene for free wekwikgene.wllsb.edu.cn/publications... 020
piatkevich.bsky.social @piatkevich.bsky.social · 25/07/2025hCRAFi-CCR2 is a new chemokine receptor activation-based fluorescent indicator for monitoring spatiotemporal dynamics of CCR2 ligands, including human/mouse CCL2, and CCL7, as well as mouse CXCL1, in culture and in vivo www.nature.com/articles/s41... 030
piatkevich.bsky.social @piatkevich.bsky.social · 04/07/2025PSmOrange3 works well for long-term mitochondria tracking in cultured mammalian cells. Plasmids for PSmOrange3 can be requested for free from @WeKwikGene here wekwikgene.wllsb.edu.cn/publications... 010
piatkevich.bsky.social @piatkevich.bsky.social · 02/07/2025Temporal domain multiplexing enables multiplexed imaging in a single channel with spectrally similar FPs using per-pixel unmixing based on differences in photobleaching rates. To facilitate this approach, we developed new far-red FPs www.biorxiv.org/content/10.1... 020
piatkevich.bsky.social @piatkevich.bsky.social · 18/06/2025mScarlet3-H (aka mYongHong) is on the cover of Nature Methods! All plasmids are available for free from WeKwikGene wekwikgene.wllsb.edu.cn/publications... 13316
piatkevich.bsky.social @piatkevich.bsky.social · 10/06/2025Using mBaoJin derivative, Peng Zou lab engineered highly photostable green eFRET GEVI, named Vega, which exhibits more than 20-fold slower photobleaching than Ace-mNeon2, and allows for long-term voltage imaging in brain slice and in vivo in mice biorxiv.org/content/10.1... 030
piatkevich.bsky.social @piatkevich.bsky.social · 05/06/2025Xue Han lab from BU used ElectraOFF to achieve routine cellular-resolution imaging over tens of minutes, occasionally up to eighty minutes, in behaving mice with minimal signal loss across neuron types and brain regions www.biorxiv.org/content/10.1... 010
piatkevich.bsky.social @piatkevich.bsky.social · 04/06/2025Hour-long voltage imaging in behaving mice! Capitalizing on the high photostability and brightness of mBaoJin, we developed eFRET GEVIs, named ElectraOFF, which extends reliable single spike detection at cellular resolution beyond one hour biorxiv.org/content/10.1... 011
piatkevich.bsky.social @piatkevich.bsky.social · 20/05/2025Due to its high chemical stability and brightness, mBaoJin is a perfect FP for Expansion Microscopy. Three-dimensional view of expanded HeLa cell coexpressing H2B-mTurquoise2 and Mito (COX8Ax2)-mBaoJin (www.nature.com/articles/s41...) 060
piatkevich.bsky.social @piatkevich.bsky.social · 19/05/2025Soma-targeting of GECIs significantly improves image segmentation, as can be seen from these examples of single-plane 2-photon imaging for FRCaMPi, jRGECO1a, and their soma-localized variant from L2/3 neurons of primary visual cortex in awake mouse journals.plos.org/plosbiology/... 010
piatkevich.bsky.social @piatkevich.bsky.social · 09/05/2025mScarlet3-H (aka mYongHong) makes a great pair with mBaoJin (nature.com/articles/s41...) for long-term live cell imaging. All plasmids can be requested for free from WeKwikGene wekwikgene.wllsb.edu.cn 050
piatkevich.bsky.social @piatkevich.bsky.social · 08/05/2025A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution in mice and zebrafish journals.plos.org/plosbiology/... 000
piatkevich.bsky.social @piatkevich.bsky.social · 03/05/2025All plasmids for FRCaMPis and SomaFRCaMPi are avalible from WeKwikGene wekwikgene.wllsb.edu.cn/publications...wekwikgene.wllsb.edu.cnWeKwikGene 072
piatkevich.bsky.social @piatkevich.bsky.social · 22/04/2025Great News & Views article by Ben Campbell covering recent development in FP field with focus on mScralet3-H (aka mYongHong) www.nature.com/articles/s41... 042
piatkevich.bsky.social @piatkevich.bsky.social · 18/04/2025mScarlet3-H (aka mYongHong), a highly stable monomeric red fluorescent protein, works well for long-term super-resolution imaging, rapid tissue clearing, and CLEM www.nature.com/articles/s41...nature.comA highly stable monomeric red fluorescent protein for advanced microscopy - Nature MethodsThe red fluorescent protein mScarlet3-H is bright, photostable and very robust to high temperature, chaotropic conditions and oxidative environments. mScarlet3-H works well in correlative light and el... 093
piatkevich.bsky.social @piatkevich.bsky.social · 02/04/2025We are very happy to see another orange flame lit up for our Electra2 plasmids on Addgene! Btw these plasmids can be also requested for free from WeKwikGene wekwikgene.wllsb.edu.cn/publications... 000
piatkevich.bsky.social @piatkevich.bsky.social · 25/02/2025New applications of Electra2! Patel et al. used Electra2 to screen for genetically modified nematodes to study dopamine signaling driving skin invasion (www.biorxiv.org/content/10.1...). All plasmids for Elctras can be requested from WeKwikGene wekwikgene.wllsb.edu.cn/publications... 010
piatkevich.bsky.social @piatkevich.bsky.social · 22/02/2025Whole-ExM for super-resolution imaging of entire embryonic and neonatal mouse bodies under conventional confocal microscopes pubs.acs.org/doi/full/10.... 020
piatkevich.bsky.social @piatkevich.bsky.social · 18/02/2025We are very excited to see the first orange flame lit up for our plasmid pHybrid-Electra2 on Addgene, which is designed for gene expression in E. coli and mammalian cells www.nature.com/articles/s41...nature.comDual-expression system for blue fluorescent protein optimization - Scientific ReportsScientific Reports - Dual-expression system for blue fluorescent protein optimization 041
piatkevich.bsky.social @piatkevich.bsky.social · 14/02/2025monArch, a near-infrared fluorescent voltage indicator, is developed via directed molecular evolution in HEK cells and characterized by several-fold higher baseline fluorescence compared to other Arch-based GEVIs. Plasmids are available from WeKwikGene wekwikgene.wllsb.edu.cn/publications... 0102
piatkevich.bsky.social @piatkevich.bsky.social · 04/02/2025A Sensitive Soma-localized Red Fluorescent Calcium Indicator for Multi-Modality Imaging of Neuronal Populations In Vivo. It works well with wide-field imaging, two-photon microscopy, and fiber photometry biorxiv.org/content/10.1... 04618
piatkevich.bsky.social @piatkevich.bsky.social · 16/01/2025Year in review 2024 from @naturemethods ! I am very excited about the advancement in ultrastable monomeric GFPs we saw in 2024 and even more excited about what is coming next. nature.com/articles/s41... 010
piatkevich.bsky.social @piatkevich.bsky.social · 08/01/2025Orthogonal RNA replication enables directed evolution and Darwinian adaptation in mammalian cells. Relevant plasmids are available from WeKwikGene wekwikgene.wllsb.edu.cn/publications... 000
piatkevich.bsky.social @piatkevich.bsky.social · 05/01/2025Novel genetically encoded red potassium indicators (RGEPOs) enable real-time visualization of both intracellular and extracellular potassium ion (K⁺) fluctuations in live tissues, allowing potassium dynamics to be imaged during seizures in mice for the first time www.biorxiv.org/content/10.1... 36314
piatkevich.bsky.social @piatkevich.bsky.social · 11/12/2024WeKwikGene is constantly improving to enhance the user experience. We’ve released a set of updates to improve navigation and allow users to view plasmids based on Lab and Publication, making material searches easier. wekwikgene.wllsb.edu.cn/plasmids/000... 120
