iiinova — where your digital self keeps evolving

Johannes Hohlbein @hohlbeinlab.bsky.social · 04/10/2026

Have an M2 Mac Book Air 15. Still very happy with it after 3+ years. Only downside: no HDMI, you know, universities and their projectors.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 18/09/2026

How is it going? Problems with prototype autofocus/focus stabilization performance => ask microscopy company to demo their microscope body with (perfect) focus stabilization => focus stabilization unit apparently currently broken awaiting repair... Kafka is calling.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 13/09/2026

(Cross post LinekdIn) V0.12.1 of #webSMLM released with inbuilt terminal functionality and experimental #smFRET (ALEX-TIRF) implementation.

GUI web interface of #webSMLM showing time traces and E/S histograms of smFRET data

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Johannes Hohlbein @hohlbeinlab.bsky.social · 07/09/2026

But, is the question already settled whether to fill in pre-heated water or not?

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Reposted by Johannes Hohlbein

Christoph Dellago @chhdellago.bsky.social · 07/09/2026

If you are like me, you start every morning by making yourself a coffee with a moka pot. ☕️ While you wait for the coffee to bubble up, you can now explore how it works: compphys.univie.ac.at/Moka I made this (thank you, Claude!) for my thermo and stat phys course, but perhaps you enjoy it too.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 07/09/2026

I am in the Netherlands at an altitude below 0 m... :) Great work!

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Johannes Hohlbein @hohlbeinlab.bsky.social · 05/09/2026

(LinkedIn cross post) 0.12.0 of #webSMLM released. Work mainly by @kjamartens.bsky.social introduced live streaming and real time analysis from frames recorded in MicroManager or Python. hohlbeinlab.github.io/webSMLM/webS...

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Johannes Hohlbein @hohlbeinlab.bsky.social · 25/08/2026

Also ask your university, the 350-400k doesn't even cover the "costs"... PhD candidates from outside (e.g., companies) are more in the 600k to 700k bracket.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 21/08/2026

To test, open hohlbeinlab.github.io/webSMLM/webS... on desktop or mobile. Everything stays local, no upload, no call back, no additional downloads. Details on github.com/HohlbeinLab/... (2/2)

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Johannes Hohlbein @hohlbeinlab.bsky.social · 21/08/2026

(LinkedIn cross post) 0.11.3 of #webSMLM released. Since 0.11.0 we added: headless spectral SMLM runs, *.ND2 loading, single particle tracking (sptPALM, Nile red PAINT, etc), light (see below), dark or high contrast mode; *.svg export of plots, change to MIT license,... (1/2)

User interface of webSMLM, center window histogram of diffusion coefficients of Nile Red diffusing in bacterial cell wall, right window bacterial cell outlines of L. actis stained with Nile Red.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 17/08/2026

Update: #webSMLM (V0.11.0), a browser-based single-molecule localisation microscopy analysis tool, includes 2D/3D phasor/MLE fit, background correction, simulations, 3D drift correction, fast temporal median filtering, rendering, spectrally resolved SMLM (!),.. hohlbeinlab.github.io/webSMLM/webS...

Pic shows STORM data of fixated Cos7 cells with CF660-labeled clathrin and CF680-labeled tubules (cells: Lorenzo Albertazzi's lab). Colour coding represents emission wavelength dependent distance between 0th and 1st order.

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Glen Dsouza @nonresidentdesi.bsky.social · 10/08/2026

📣 We’re hiring a postdoc for @hot-mes-asu.bsky.social ! 🦠🔬 We’re looking for someone excited about microbial interactions, ecology & evolution, and using molecular genetics and microfluidics to understand microbial communities. Come do fun science with us! apply.interfolio.com/190859

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Johannes Hohlbein @hohlbeinlab.bsky.social · 20/07/2026

Want to analyse SMLM data on your phone (or any other browser)? Here we go: hohlbeinlab.github.io/webSMLM/webS..., repo on GitHub #webSMLM

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Johannes Hohlbein @hohlbeinlab.bsky.social · 19/07/2026

Water is chilled but without bubbles afaik. :(

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Johannes Hohlbein @hohlbeinlab.bsky.social · 19/07/2026

Life hack: look out for the free drinking water dispensers. Hidden but there.

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Luke Lavis @rhodamine110.bsky.social · 17/07/2026

Excited to see this collaboration now in print. Dye chemistry meets AI-designed proteins. Three small, orthogonal tags; lifetime modulation; split versions. Request the free dyes at dyes.janelia.org www.science.org/doi/10.1126/...

science.org

De novo design of orthogonal far-red, orange, and green fluorophore-binding proteins for multiplexed imaging

Fluorescent proteins and small-molecule dyes offer complementary advantages for biological imaging: proteins are amenable to genetic tagging, whereas dyes provide superior brightness and photostabilit...

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Dr Dan Jones @hookean.bsky.social · 10/07/2026

Here is the Physics Worksheet Generator. It has taken some time to get to this stage, but it currently covers GCSE and A-Level for most exam boards. Feedback welcome for errors or improvements. I will continue to work on it. I hope you find it useful #iTeachPhysics www.drjonesphysics.com/pwg

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Johannes Hohlbein @hohlbeinlab.bsky.social · 10/07/2026

Nothing fancy, just some old sample doing things earlier today. #FlourescenceFriday

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Johannes Hohlbein @hohlbeinlab.bsky.social · 10/07/2026

And you weren’t asked to upload in advance?

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Johannes Hohlbein @hohlbeinlab.bsky.social · 05/07/2026

Enjoining the @adamtooze.bsky.social sub stack: art, politics, economics, history,... Many links, much food for thought.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 01/07/2026

Thanks. We’ll look into it further.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 01/07/2026

So where is the heavy lifting taking place? On a server or in the browser? Have been installing python stuff with students for an hour today. Want browser magic at least for courses.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 01/07/2026

Was wondering about that. What framework are you using?

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Johannes Hohlbein @hohlbeinlab.bsky.social · 29/06/2026

Magic Carpet from Bullfrog (1994).

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Johannes Hohlbein @hohlbeinlab.bsky.social · 28/06/2026

Sad but true. And I used to make fun of Americans setting their ACs to ridiculously low temperatures.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 28/06/2026

This pie chart though. Would recommend a proper program to do the numbers justice. Compare the slice of nuclear (1.6% apparently or not?) with the size of all other slices…

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Johannes Hohlbein @hohlbeinlab.bsky.social · 27/06/2026

Also das ist wohl auch zu erwarten bei Inkubation zu Zimmertemperatur (Lactococcus lactis anstelle Lactobacillus delbrueckii). Pre-heating bei yoghurt ist auch nötig, um die Molkeproteine zu denaturieren um cremigen Joghurt zu bekommen.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 27/06/2026

Update 11 Uhr: starke Buttermilchvibes.

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Reposted by Johannes Hohlbein

Cees Dekker @ceesdekker.bsky.social · 16/06/2026

WORK: our Bionanoscience department at TU Delft is looking to hire an Assistant Professor in Biophysics of Living Systems. Apply! Deadline 30 July careers.tudelft.nl/job/Delft-As... RT=nice

careers.tudelft.nl

Assistant Professor in Biophysics of Living Systems

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Dr Dan Jones @hookean.bsky.social · 27/06/2026

I have created over 100 free apps and simulations covering all major GCSE and A-Level Physics topics. Explore them here: #iTeachPhysics #Science 🧪 www.drjonesphysics.com/apps

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Johannes Hohlbein @hohlbeinlab.bsky.social · 26/06/2026

Experiment angesetzt. 23 Uhr, 27 grad, 250 ml Milch aus Kühlschrank, 1 TL eine Woche alten Sauerteigansatz auch aus dem Kühlschrank. Frischhaltefolie aufs Glas und Gute Nacht.💤

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Johannes Hohlbein @hohlbeinlab.bsky.social · 26/06/2026

Könnte man die Milch auch mit einem Sauerteigstarter impfen? Ach, einfach mal probieren.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 24/06/2026

Noticeable in the dark when trying to focus on a blue next to a red light.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 20/06/2026

Yes, and I would prefer those numbers to be above 100 :) . Yet people have been reluctant to send push requests or suggest additions.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 19/06/2026

You might have heard about our GitHub repository on #OpenMicroscopy. I finally vibecoded a page that (a) allows to search all entries and (b) generates a new database as soon as items are added on GitHub: hohlbeinlab.github.io/OpenMicrosco... Check it out and comments welcome!

hohlbeinlab.github.io

Open Microscopy — Resource Index

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Jens Schmidt @jenscs83.bsky.social · 11/06/2026

I am looking to recruit 2 postdoctoral fellows to the lab to work on a recently funded project focused on class switch recombination in B cells. If you are interested in immunology, gene expression, and RNA and protein single molecule imaging, please reach out (schmi706@msu.edu). Please share!

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Johannes Hohlbein @hohlbeinlab.bsky.social · 11/06/2026

There is the concept of hyperfocal distance in photography, but not sure whether this is relevant here.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 09/06/2026

Please remove my name from the list…

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Reposted by Johannes Hohlbein

Naomi Chrispijn-Steenbeek @naomichrispijn.bsky.social · 08/06/2026

Join the NWO Physics of Life Seminar on 19 June at Utrecht Science Park Talks by Anna Erzberger (EMBL), Christian Kaiser (UU), Felix Frey (TU/e), Justin Tauber (WUR) Iris Smokers (MPI-CBG) Register <15 June shorturl.at/0VGb7 Organized by @floberger.bsky.social and @uddaloksen.bsky.social

shorturl.at

NWO Physics of Life seminar | NWO

The NWO physics of life research community committee welcomes you for a new seminar on June 19, 2026, at Utrecht Science Park. The seminar has an international keynote speaker, dr. Anna Erzberger (EMB...

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Johannes Hohlbein @hohlbeinlab.bsky.social · 28/05/2026

Yes. Mauritshuis in The Hague, highly recommended! en.wikipedia.org/wiki/Maurits...

en.wikipedia.org

Mauritshuis - Wikipedia

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Johannes Hohlbein @hohlbeinlab.bsky.social · 22/05/2026

"A complete MOSAIC CAD model, bill of materials, and nearly 1,000 pages of installation, maintenance and operation instructions are available..." :)

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Johannes Hohlbein @hohlbeinlab.bsky.social · 19/05/2026

Ordering stuff for the lab is fun. Now I have white board markers but no white board and, even better, two new displays yet the delivery of the PCs got delayed to September 2026... Uff.

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Lorenzo Olivi @loreoliv.bsky.social · 11/05/2026

🧵 New pre-print out from my time at @mib-wur.bsky.social showing how the titration of the initiator protein DnaA on the chromosome of E. coli can generate stable DNA replication @hohlbeinlab.bsky.social @nicoc-micsynmet.bsky.social doi.org/10.64898/202...

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Johannes Hohlbein @hohlbeinlab.bsky.social · 07/05/2026

The "Witch of Agnesi" is not what I thought it would be. According to Wikipedia: a cubic plane curve defined from two diametrically opposite points of a circle.

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