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Jan Gawor

@gaworjan.bsky.social
846 followers 520 following 47 posts

Sanger / NGS sequencing specialist (love long reads :) / microbial de novo sequencing / Research Associate at oligo.pl DNA Sequencing Facility IBB PAS Warsaw, Poland

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Jan Gawor @gaworjan.bsky.social · 16/09/2026
Our research is out in Microbial Ecology! We investigated the diet and gut microbiota of the Antarctic fairy shrimp (Branchinecta gaini) across postglacial ponds on King George Island using 16S & 18S rRNA gene metabarcoding. 🧵👇
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Jim Shaw @jimshaw.bsky.social · 15/09/2026
The sylph metagenome profiler is v1.0.0! sylph-docs.github.io A new DB format + approach --> huge performance gains: GTDB-R232 (200k species) now takes < 5 GB of RAM and ~30s (2GB fq.gz). Huge thanks to @benjwoodcroft.bsky.social and his ongoing performance efforts (github.com/wwood/weebill)
sylph-docs.github.io
Documentation for sylph - ultrafast, precise metagenomic profiling
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Marta Gawor @myogeek.bsky.social · 14/07/2026
Looking for a #PhD student! 🧬 Join an international project researching the role of myosin VI in chromatin remodelling & cellular biomechanics in skeletal muscle #science. Driven & passionate about research? Check the link for project details (no. 04) & registration 👇" nencki.edu.pl/admission-to...
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Earth BioGenome Project 🌍 @ebpgenome.bsky.social · 14/07/2026
❄️🧬 Can we generate high-quality reference genomes without the cold chain? A new study suggests the answer is yes. DNA preserved at room temperature for up to one week produced chromosome-scale reference genomes. 📖 tinyurl.com/3zwtj9ka #Genomes @gigascience.bsky.social @sangerinstitute.bsky.social
Close-up photograph of a mosquito perched on a green blade of grass against a dark background. White text at the top reads, "Breaking the Cold Chain." Additional text states, "Room-temperature preservation enables chromosome-scale reference genomes." "GigaScience 2026" appears in the lower-right corner.Three researchers collect biological samples in a forest. One researcher kneels while holding a collection tube beside an insulated cooler, and two others stand nearby preparing samples. A large arrow points to the cooler. Text explains that high-quality reference genomes traditionally require specimens to remain frozen from collection through DNA extraction, highlighting the challenge of maintaining the cold chain during fieldwork.
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Ben Langmead @benlangmead.bsky.social · 22/06/2026
Movi 2 has appeared (as an advance article) in Bioinformatics 🧬 Faster, leaner pangenome queries — half the memory of Movi 1, ~30% faster. Paper: academic.oup.com/bioinformati... Code: github.com/mohsenzakeri/Movi (1/6)
academic.oup.com
Validate User
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Stephen Turner @stephenturner.us · 26/03/2026
The AI Scientist paper is out in Nature today. Runs the full ML research pipeline: idea generation, literature search, coding experiments, running them via agentic tree search, writing the paper in LaTeX, automated review. Towards end-to-end automation of AI research www.nature.com/articles/s41...
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Jan Gawor @gaworjan.bsky.social · 09/02/2026
Super cool!
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Jan Gawor @gaworjan.bsky.social · 13/01/2026
🎉 Our first paper this year is out! We show that treated wastewater microbiomes reflect operational changes in WWTPs, using 16S rRNA (DNA & cDNA) and FISH. 24-h composite sampling gives highly representative profiles. 🧬 #Microbiome #NGS #Wastewater www.nature.com/articles/s41...
nature.com
Client Challenge
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Torsten Seemann @torstenseemann.bsky.social · 22/12/2025
💾 mlst 2.25.0 has been released! Fixed a bug that missed alleles in increasingly larger schemes like senterica (thanks @microbiologikat.bsky.social) and updated the databases. #bioinformatiocs #microbiology #genomics github.com/tseemann/mls...
github.com
Releases · tseemann/mlst
:id: Scan contig files against PubMLST typing schemes - tseemann/mlst
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Torsten Seemann @torstenseemann.bsky.social · 13/12/2025
💾 Shovill 1.4.1 has been released! The best way to de novo assemble microbial genomes from Illumina FASTQ. Major fixes to the SKESA module, plasmid mode for the Spades module, and more error checking. #bioinformatiocs #genomics #microbiology github.com/tseemann/sho...
github.com
Releases · tseemann/shovill
⚡♠️ Assemble bacterial isolate genomes from Illumina paired-end reads - tseemann/shovill
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plantgenomics.bsky.social @plantgenomics.bsky.social · 19/11/2025
Efficient near telomere-to-telomere assembly of Nanopore Simplex reads doi.org/10.1101/2025... Cookbook for Plant Genome Sequences www.preprints.org/manuscript/2...
doi.org
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Olga Anczukow @olgaanczukow.bsky.social · 14/11/2025
Join us at this exciting Workshop on #LongReads with keynotes from @aphillippy.bsky.social @mydennis.bsky.social co-organized by @christinebeck.bsky.social and Mark Adams @jacksonlab.bsky.social Register www.jax.org/longread #RNA #genomics #bioinformatics #cancer #medicine #evolution #neuroscience
jax.org
Long-Read Sequencing Workshop
The program will involve sessions on Genome Evolution, Genome Regulation, Transcriptomics, Rare Diseases, Common Diseases, and Viral and Microbial Genomes. Committed speakers include Adam Phillipy (NH...
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Jaebeom Kim @jbeom.bsky.social · 16/10/2025
Easy and interactive taxonomic profiling with Metabuli App. It integrates database curation, read QC, taxonomic profiling, and visualization right on your desktop. No command line, server, or internet required. Now published in Bioinformatics! 🧵1/5 doi.org/10.1093/bioi... github.com/steineggerla...
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Jan Gawor @gaworjan.bsky.social · 17/10/2025
Quite interesting pipeline for bacterial isolate QC including de novo assembly and contamination detection. github.com/B-UMMI/INNUca
github.com
GitHub - B-UMMI/INNUca: INNUENDO quality control of reads, de novo assembly and contigs quality assessment, and possible contamination search
INNUENDO quality control of reads, de novo assembly and contigs quality assessment, and possible contamination search - B-UMMI/INNUca
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Ben Adler @benadler.bsky.social · 01/10/2025
Today in @nature.com , we highlight how a cousin of CRISPR-Cas10, mCpol, establishes an evolutionary trap in anti-phage immune systems. Check out @erinedoherty.bsky.social and my work from @doudna-lab.bsky.social lab here: www.nature.com/articles/s41...
nature.com
A miniature CRISPR–Cas10 enzyme confers immunity by inhibitory signalling - Nature
Panoptes, an anti-phage defence system against virus-mediated immune suppression, is revealed.
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Tami Lieberman @contaminatedsci.bsky.social · 29/09/2025
Precisely calling mutations across hundreds of bacterial isolates has been hard, requiring manual filtering and expertise. Until now, using AccuSNV. Herui Liao trained an ML model based on our previous meticulously called SNVs. www.biorxiv.org/content/10.1...
biorxiv.org
High-accuracy SNV calling for bacterial isolates using deep learning with AccuSNV
Accurate detection of mutations within bacterial species is critical for fundamental studies of microbial evolution, reconstructing transmission events, and identifying antimicrobial resistance mutati...
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Agata Starosta @agatastarosta.bsky.social · 29/09/2025
Nauka i badania, w kraju, który jest 20-tą gospodarką świata, powinny być priorytetem ponad podziałami. Apelujemy do wszystkich, którzy rozumieją potrzebę prowadzenia badań naukowych w Polsce o zapoznanie się z treścią listu i poparcie Apelu. www.petycjeonline.com/list_otwarty...
petycjeonline.com
List otwarty do Prezydenta RP, Marszałka Sejmu RP, Marszałka Senatu RP,  Prezesa Rady Ministrów oraz Wiceprezesów Rady Ministrów w sprawie finansowania nauki, w tym zwłaszcza Narodowego Centrum Nauki
Szanowni Pa&#324;stwo, W niniejszej petycji prezentujemy List otwarty, kt&oacute;ry b&#281;dzie wys&#322;any do Kancelarii Prezydenta, Marsza&#322;k&oacute;w Sejmu i Senatu, Prezesa Rady Ministr&oacut...
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Jan Gawor @gaworjan.bsky.social · 25/09/2025
Our paper is out! We report the first genome-wide essential gene map of Streptococcus anginosus 980/01, a human commensal & opportunistic pathogen. Using TnSeq, we found 348 essential genes, incl. 40 unique ones, shedding light on its biology & pathogenic potential. www.nature.com/articles/s41...
nature.com
Genome-wide identification of essential genes in the invasive Streptococcus anginosus strain - Scientific Reports
Scientific Reports - Genome-wide identification of essential genes in the invasive Streptococcus anginosus strain
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Dr. Jennifer Pett-Ridge @jeffinerca.bsky.social · 23/09/2025
Microbiome sequencing has come so far in 2 decades. In my PhD— I used TRFLP…. am now doing long read sequencing of entire soil metagenomes! This review was great fun- thanks Gitta Szabó @emileyeloe-fadrosh.bsky.social Tanja Woyke of @jgi.doe.gov www.nature.com/articles/s41...
nature.com
A genomic view of Earth’s biomes - Nature Reviews Genetics
Genome-wide approaches have uncovered the vast microbial and viral diversity across ecosystems. This Review explores advances in metagenomics, single-cell sequencing and functional profiling to elucid...
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Javier Santoyo @jsantoyo.bsky.social · 01/09/2025
GenMasterTable: a user-friendly desktop application for filtering, summarising, and visualising large-scale annotated genetic variants. #GeneticVariants #VariantExploration #DataVisualization #GUI #BMCbioinformatics 🧬 🖥️ bmcbioinformatics.biomedcentral.com/articles/10....
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Tami Lieberman @contaminatedsci.bsky.social · 15/08/2025
Our high-precision metagenomic strain caller, PHLAME, is now published in Cell Reports!! www.cell.com/cell-reports... PHLAME works on tough sample types -- including those with coexisting strains of a species and low depth.
cell.com
Intraspecies associations from strain-rich metagenome samples
Qu et al. present PHLAME, a method to analyze strain composition in metagenomic samples where many strains of the same species coexist. Using PHLAME, they show relationships between strain abundances ...
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Pat Schloss @patschloss.bsky.social · 31/07/2025
I'm offering a new workshop that's part instruction, part group coaching. This program will focus on the design principles we should consider when generating visuals. There won't be any coding so don't let that hold you back shop.riffomonas.org/products/tra...
shop.riffomonas.org
Transform your data visualizations in 5 weeks - no judgement, no code
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Anthony Beckhouse @antobeck.bsky.social · 16/07/2025
The software updates on NovaSeq X Series really do improve performance, quality and output. It's all documented in the #illumina blog- sapac.illumina.com/science/geno...
sapac.illumina.com
NovaSeq X software update 1.3 improves data quality and customer usability
The first shipments of the NovaSeq X Series introduced XLEAP-SBS chemistry on Illumina’s next-generation high-throughput platform. With the 25B flow cell, NovaSeq X software update 1.2 enabled up to 1...
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🧬Jacob L Steenwyk @jlsteenwyk.bsky.social · 14/07/2025
NEW publication: Super happy to see the ClipKIT web application now published in Nucleic Acids Research @narjournal.bsky.social!! Trim your alignments for FREE on us :D Application: clipkit.genomelybio.com#/ Manuscript: academic.oup.com/nar/article/... #phylogenetics #evolution #bioinformatics
academic.oup.com
ClipKIT in the browser: fast online trimming of multiple sequence alignments for phylogenetics
Abstract. Multiple sequence alignment trimming can help improve phylogenetic signal and reduce computational load. ClipKIT trims multiple sequence alignmen
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Jan Gawor @gaworjan.bsky.social · 14/07/2025
Our paper is out!🦠 The S. aureus phage ASZ22RN can infect or lyse all tested strains and efficiently transfer plasmids—even in strains with phage defenses. Its unique ability to bypass intracellular barriers makes it a valuable tool for genetic delivery into clinical isolates.
pubmed.ncbi.nlm.nih.gov
Characterization of a novel Phietavirus genus bacteriophage and its potential for efficient transfer of modified shuttle plasmids to Staphylococcus aureus strains of different clonal complexes - PubMe...
This study highlights the capacity of a newly isolated staphylococcal <i>Phietavirus</i>, ASZ22RN, to transfer a low-copy-number shuttle <i>Staphylococcus aureus</i>-<i>Escherichia coli</i> plasmid to...
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Jan Gawor @gaworjan.bsky.social · 10/06/2025
Delighted to share a new preprint from the lab! We identified essential genes in S. anginosus, a bloodstream isolate, under optimal laboratory conditions using a transposon mutagenesis combined with Transposon-Directed Insertion Site Sequencing (TraDIS). www.biorxiv.org/content/10.1...
biorxiv.org
Genome-Wide Identification of Essential Genes in the Invasive Streptococcus anginosus Strain
Background: Streptococcus anginosus, part of the Streptococcus anginosus group (SAG), is a human commensal increasingly recognized as an opportunistic pathogen responsible for abscesses formation and ...
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Shicheng Guo @shihcheng.bsky.social · 28/05/2025
Explore microbial evolution with AutoMLST2, a web server for automated phylogenetic analysis for bacterial & archaeal genomes. PMID:40357641, Nucleic Acids Res 2025, @NAR_Open doi.org/10.1093/nar/gkaf397 #Medsky #Pharmsky #RNA #ASHG #ESHG 🧪
doi.org
https://doi.org/10.1093/nar/gkaf397
No description available
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Vikram Shivakumar @vikramshivakumar.bsky.social · 09/05/2025
Excited to share our latest work on comparing and visualizing multiple genome assemblies to identify conservation and structural variation in pangenomes with Mumemto! Check out poster 250 at #bog25 if you are here. New preprint coming very soon 👀
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iimcb.bsky.social @iimcb.bsky.social · 16/04/2025
🌐Our @nature.com paper is out! Researchers from IIMCB and other Polish institutions show how TENT5A boosts mRNA vaccine efficacy by stabilizing therapeutic mRNAs and enhancing antigen production. A step toward better therapies for cancer and beyond! 🧬 👉Read the story: bit.ly/3XYvQkI #Nature #mRNA
bit.ly
IIMCB
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Jan Gawor @gaworjan.bsky.social · 24/03/2025
The best food choices for healthy aging! Huge cohort - 105,000 people tracked over 30 years. Eat fruit and vegetables and avoid red meat. Wine is quite good. www.nature.com/articles/s41...
nature.com
Optimal dietary patterns for healthy aging - Nature Medicine
In two longitudinal cohorts followed for 30 years, the associations of eight different dietary patterns with healthy aging—encompassing cognitive, physical and mental health—were studied, identifying ...
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Jan Gawor @gaworjan.bsky.social · 23/03/2025
Our first paper this year is out! We studied the occurrence and diversity of fungi on the skin of Atopic dermatitis patients and healthy volunteers by using a two-pronged strategy (culture-dependent and NGS-based). onlinelibrary.wiley.com/doi/10.1111/...
onlinelibrary.wiley.com
The Skin Mycobiome of Patients With Atopic Dermatitis and Healthy Volunteers: A Case–Control Study
Atopic dermatitis (AD) is a common inflammatory skin disease, for which dysbiosis of the skin mycobiome is considered a triggering factor. The aim of this study was to explore the skin mycobiome of A...
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Jan Gawor @gaworjan.bsky.social · 05/03/2025
Has anyone of you know how to speed up docker compose? I run the Phastest phage identification pipeline in docker and it is really slow due to running on single thread. Interestingly first sample in my batch is runing faster on multiple cores. Any hints?
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Eric Topol @erictopol.bsky.social · 12/02/2025
A link between yogurt intake and reduce risk of colon cancer, from cohort of over 130,00 participants, with inverse relationship to Bifidobacterium in gut microbiome www.tandfonline.com/doi/full/10.... open-access
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Stephen Turner @stephenturner.us · 29/01/2025
Highly multiplexed spatial transcriptomics in bacteria www.science.org/doi/10.1126/science…
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Mike Sowden @mikeachim.bsky.social · 27/01/2025
In 2014 Dutch scientists left a hamster wheel outside, to see if wild animals would use it like their domesticated counterparts. The answer: hell yes! 734 visits from wild mice - plus rats, shrews, slugs ("running" being subjective here) & even frogs and snails. The apparent reason: fun. Just fun.
Chart showing instances of wild animals using a hamster wheel left out in the wild, with visits dominated by wild mice, followed by slugs, rats, shrews and a few frogs and snails.
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Jan Gawor @gaworjan.bsky.social · 24/01/2025
After shuttting down 454 in 2013, Roche is trying to get back into the sequencing market with its soon-to-be-released sequencer.
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Petr Svoboda @svobodalab.bsky.social · 24/01/2025
I have one open PhD position. Maybe one of the last PhD positions in the lab as I'm planning changes in what we do and how we will do it some five years from now. I'm offering freedom (pun intended), respect, an interesting project, and a good full time contract w. health insurance.
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Thom Booth @thombooth.bsky.social · 22/01/2025
getphylo is finally official! Build phylogenetic trees directly from genbank files - at breakneck speed! I'd like to thank everyone who provided feedback and encourage you to continue. Improving getphylo doesn't stop now the paper is out! 1/5 🧪 bmcbioinformatics.biomedcentral.com/articles/10....
bmcbioinformatics.biomedcentral.com
getphylo: rapid and automatic generation of multi-locus phylogenetic trees - BMC Bioinformatics
Background The increasing amount of genomic data calls for tools that can create genome-scale phylogenies quickly and efficiently. Existing tools rely on large reference databases or require lengthy d...
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Jan Gawor @gaworjan.bsky.social · 22/12/2024
Our paper is out! We have sequenced the plastid genomes of 13 Prototheca strains and reconstructed a comprehensive phylogeny that reveals evolutionary patterns within the genus and among its closest relatives. bmcbiol.biomedcentral.com/articles/10....
bmcbiol.biomedcentral.com
Reduced plastid genomes of colorless facultative pathogens Prototheca (Chlorophyta) are retained for membrane transport genes - BMC Biology
Background Plastids are usually involved in photosynthesis, but the secondary loss of this function is a widespread phenomenon in various lineages of algae and plants. In addition to the loss of genes...
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Jan Gawor @gaworjan.bsky.social · 12/12/2024
What are your favourite tools for visualizing Metaphlan or kraken2 results? I just found this one: github.com/acvill/brack... There is even shiny app: acvill.shinyapps.io/bracken_plot/
github.com
GitHub - acvill/bracken_plot: A Shiny app for taxonomic abundance visualization
A Shiny app for taxonomic abundance visualization. Contribute to acvill/bracken_plot development by creating an account on GitHub.
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Jan Gawor @gaworjan.bsky.social · 10/12/2024
Today I had opportunity to see the newest @illumina MiSeq i100 Plus. The doors were opened:
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M.J. Crockett @mjcrockett.bsky.social · 01/12/2024
Is it Bad to leave Twitter? No. Here are 7+ years of insights from my lab’s research that explain why. Featuring work w/ @williambrady.bsky.social @killianmcloughlin.bsky.social 🧵
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Jan Gawor @gaworjan.bsky.social · 01/12/2024
EasyPubPlot - Easy and Publishable Plotting pharmaco-omicslab.shinyapps.io/EasyPubPlot/
pharmaco-omicslab.shinyapps.io
EasyPubPlot
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Jan Gawor @gaworjan.bsky.social · 29/11/2024
Check this out👇 Another bacterial genome comparison tool. It even works on Windows!
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Jan Gawor @gaworjan.bsky.social · 28/11/2024
Archaeome - the dark matter of the microbiome. asm.org/articles/202...
asm.org
The Archaeome: An Emerging Player in Health and Disease | ASM.org
Archaea are the least understood members of the microbiome. However, as tools for studying microbes improve, the archaeome—and its association with health and disease—is coming into focus.
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Jan Gawor @gaworjan.bsky.social · 28/11/2024
I was looking for alternative 16S database to SILVA and found MIMt. Has anyone tested it yet? environmentalmicrobiome.biomedcentral.com/articles/10....
environmentalmicrobiome.biomedcentral.com
MIMt: a curated 16S rRNA reference database with less redundancy and higher accuracy at species-level identification - Environmental Microbiome
Motivation Accurate determination and quantification of the taxonomic composition of microbial communities, especially at the species level, is one of the major issues in metagenomics. This is primari...
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Mitch Guttman @mitchguttman.bsky.social · 27/11/2024
Gene regulation involves thousands of proteins that bind DNA, yet comprehensively mapping these is challenging. Our paper in Nature Genetics describes ChIP-DIP, a method for genome-wide mapping of hundreds of DNA-protein interactions in a single experiment. www.nature.com/articles/s41...
nature.com
ChIP-DIP maps binding of hundreds of proteins to DNA simultaneously and identifies diverse gene regulatory elements - Nature Genetics
ChIP-DIP (ChIP done in parallel) is a highly multiplex assay for protein–DNA binding, scalable to hundreds of proteins including modified histones, chromatin regulators and transcription factors, offe...
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🧬Jacob L Steenwyk @jlsteenwyk.bsky.social · 25/11/2024
NEW Publication!😄 Here, we show how #PhyKIT — a broadly applicable toolkit for #phylogenomics — can be used to construct phylogenomic data matrices (& quantify biases therein), detect anomalies in predicted orthology, calculate gene-gene coevolution, & more! 🔗: tinyurl.com/yc5ja4xz
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Jan Gawor @gaworjan.bsky.social · 23/11/2024
Another great comparative genomics tool: art-egorov.github.io/lovis4u/
art-egorov.github.io
LoVis4u
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Ellie Rand @ellie-rand.bsky.social · 23/11/2024
Get ready to dance, our paper – Phage DisCo: targeted discovery of bacteriophages by co-culture – has been pre-printed! 🪩 This has been a really fun project to work on with @sianowen.bsky.social, @baym.lol, @nquinoneso.bsky.social, and our two talented undergrads Kesther and Carmen!
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