Reposted by Dan Gibbs 🌱🧬
We continue the session about E3 ligases with @djgibbs.bsky.social who is talking about his recent exciting findings about the interplay of E3 ligase NOT4 and TOR signalling (see also his recent publication on this www.nature.com/articles/s41...) #proteostasis @plantproteostasis.bsky.social
![Modified Fig. 8 | The OsRAP2.4h–PIP2 module plays a conserved role in regulating hypoxia tolerance and plant growth.
a, Seed germination of Zhonghua 11 (ZH11) and OsRAP2.4h knockout lines (Os2.4h-1 and Os2.4h-2) under normal (Air) or hypoxia (0.1% [v/v] O2) for 7 days.
b, The coleoptile length of the genotypes as presented in (a). Data are presented as means ± SD ( n = 3 independent experiments, each with 7 plants per genotype). Different letters without prime and with one prime indicate significant differences among genotypes under normal (Air) and hypoxia, respectively, at P < 0.05 (one -way ANOVA with Tukey ’s HSD test).
c, Growth phenotypes of 7-week-old ZH11, Osrap2.4h-1, and Osrap2.4h-2 under normal growth conditions.
d, The plant heights for the genotypes as presented in (c). Data are presented as means ± SD (n = 3 biological replicates). Different letters indicate significant differences among genotypes at P < 0.05 (one -way ANOVA with Tukey ’s HSD test).
e, RT-qPCR analysis of aquaporin genes OsPIP2;1 and OsPIP2;4 in 2-week-old ZH11, Osrap2.4h-1, and Osrap2.4h-2 plants. Transcripts were normalized to levels of ACTIN. The experiments were repeated three times with similar results. Data are presented as means ± SD (n = 3 independent experiments, each with three technical replicates). Different letters indicate significant differences among genotypes at P < 0.05 (one-way ANOVA with Tukey’s HSD test). Exact P values and 95% confidence intervals for all comparisons are provided in Source Data.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:tfidqyziwn7mqcunvhghjcno/bafkreignx6hblmdzagufxe5fgckhgyyxr3tohwkkd3chjmvi3hix7fck5y)
![Modified Fig. 2 | RAP2.4h is a key regulator balancing hypoxia tolerance and plant growth.
d, Phenotypes of 1-week -old WT, rap2.4h, OE-1, and OE-2 seedlings subjected to hypoxia (0.1% [v/v] O2) or normoxia (Air) in the dark for 7 h and then incubated in normoxic conditions for 4-d recovery.
e–f, Green cotyledon percentages (e) and relative chlorophyll contents (f) for the genotypes as presented in (d). For each genotype, chlorophyll content in hypoxia-treated seedlings was normalized to that of its own dark control (set to 100%). Data are presented as means ± SD ( n = 3 independent experiments, each with 5 seedlings per genotype).
g, Growth phenotypes of 5-week-old (top) and 6-week-old (bottom) WT, rap2.4h, OE-1, and OE-2 plants.
h, The number of rosette leaves in WT, rap2.4h, OE-1, and OE-2 plants at bolting. Data are presented as means ± SD (n = 3 independent experiments, each with 9 plants per genotype).
i, Phenotypes of the sixth leaf (top) of 5-week-old and main stem (bottom) of 7-week-old WT, rap2.4h, OE-1, and OE-2 plants. The statistics of leaf areas (cm2) and width of stems (mm) are shown below. Data are presented as means ± SD (n = 3 independent experiments, each with 3 plants per genotype). Different letters indicate significant differences among different genotypes at P < 0.05 (one-way ANOVA with Tukey’s HSD test). Exact P values and 95% confidence intervals for all comparisons are provided in Source Data.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:tfidqyziwn7mqcunvhghjcno/bafkreien4qdx3sxajcnjixn6jm7hc3ac5w3hmnweiygvpynbqukavitkku)
