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David Rach

@davidrach.bsky.social
431 followers 413 following 111 posts

Immunology PhD student @UMBaltimore. Views are my own. Favorite rabbit holes include #flowcytometry #rstats #bioconductor, with dashes of #coffee #kayaking #birding #ultrunning #languages. RPCV Ghana 16->18, TCK Mexico 96->11 github.com/DavidRach

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Reposted by David Rach
Erik van Nimwegen @erikvannimwegen.bsky.social · 21/08/2026
Remember this? bsky.app/profile/did:... Rigorous visualization of the structure in your high-dimensional data. Fantastic work by @dhdegroot.bsky.social With help of the reviewers of Nat Biotech it is now updated, extended an officially published here: www.nature.com/articles/s41... 1/n
nature.com
Bonsai reconstructs tree representations for distortion-free visualization and exploration of high-dimensional data - Nature Biotechnology
High-dimensional data are visualized through representation on tree structures.
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Stevie Ped @smped.bsky.social · 19/08/2026
Reminder for all members of the Bioconductor community. Nominations close at the end of the month. If you're unsure, please reach out on the Bioc chat or through your networks
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MetroFlow @metroflowcyto.bsky.social · 30/06/2026
#Metroflow2026 registration is open and for the first time we are hosting our annual meeting at Wiley, publisher of Cytometry Part A, Cytometry Part B, and Current Protocols of Cytometry. Find us at Eventbrite or in the URL below for more details! tinyurl.com/MetroFlow2026 #flowcytometry #flowcyto
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International Society for Advancement of Cytometry @isacytometry.bsky.social · 29/06/2026
We’re excited to announce the dates and location for CYTO 2028! 🎉 Save the date and stay tuned for more details as we continue building future opportunities for discovery, collaboration, and innovation within the cytometry community. CYTO 2028 June 24-28, 2028 Calgary, Alberta, Canada
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David Rach @davidrach.bsky.social · 18/06/2026
Ph.Done! youtu.be/h_G-UR-BmS4?...
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David Rach @davidrach.bsky.social · 11/06/2026
One week to go, everyone is still invited 😇😂 www.youtube.com/live/K7iae7I... #rstats #flowcytometry #bioconductor
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bioRxiv Bioinfo @biorxiv-bioinfo.bsky.social · 05/06/2026
A Reproducible and Extensible Benchmark of Supervised Cell Type Annotation Tools for Cytometry Data www.biorxiv.org/content/10.64898/20…
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David Rach @davidrach.bsky.social · 04/06/2026
Tuesday, June 9th, 5:00 PM, Hall AB - Poster # 232: My PhD supervisor Dr. Cristiana Cairo is showing data for the same cohort, using a similar semi-supervised approach, but looking at cord blood Monocytes and Dendritic cell subsets. Ask her about the sex-specific differences! 4/4
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David Rach @davidrach.bsky.social · 04/06/2026
Tuesday, June 9th, 10:30 AM, Meeting Room 2F - Parallel: Spectral and High Parameter Cytometry: I will be talking about our semi-supervised pipeline, and how we are leveraging it for greater understanding of immune heterogeneity of human Alpha Beta T cells in early life. 3/n
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David Rach @davidrach.bsky.social · 04/06/2026
Monday, June 8th, 5:00 PM, Hall AB - Poster # 245: I will be at our late-breaker poster for the Cytometry in R course. Get a look behind the curtain about what goes into the project, and what we have planned going forward. 3/n
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David Rach @davidrach.bsky.social · 04/06/2026
Sunday, June 7th, 10:30 AM, Grand Ballroom - Parallel: SRL and Education: I will be talking about our attempts at Open-Source Automation at the UMGCCC FCSR & how these efforts are paying off in our ability to detect cytometer meltdowns & badly unmixed experiments before users report issues 2/n
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David Rach @davidrach.bsky.social · 04/06/2026
Going to #CYTO2026? Same! If you don't see me in the coffee line, and are looking to add to your laptop's hex sticker collection (or just want to give me general grief 🤓), you can track me down at the following 1/n #rstats #CytometryInR #flowcytometry #Bioconductor
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Bioconductor @bioconductor.bsky.social · 06/05/2026
📅 Schedule information for BioC2026 is now available on the BioC2026 website. Registration is also officially open for the conference and the post-conference Carpentries workshop and hackathon. Early bird pricing ends on 15 June 2026. 🎟️ More details and registration: bioc2026.bioconductor.org
bioc2026.bioconductor.org
Bioconductor
Join/follow our community
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bioRxiv Cell Biology @biorxiv-cellbio.bsky.social · 21/04/2026
FlowWeb, a free, web-based platform for flow cytometry data analysis www.biorxiv.org/content/10.64898/20…
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International Society for Advancement of Cytometry @isacytometry.bsky.social · 10/04/2026
Late-Breaking Poster submissions close MONDAY! If you have new, high-impact data, now’s the time to share it with the global cytometry community. Don't miss the chance to be part of the conference experience. Submission deadline: Monday, April 13 www.cytoconference.org/abstracts"
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David Rach @davidrach.bsky.social · 11/04/2026
If you want to gate in R, both flowGate and openCyto are a little friendlier than using just base flowCore (covered in class recently: umgcccfcsr.github.io/CytometryInR...). Alternatively, floreada.io is free & web-based (and can convert into FlowJo.wsp and be brought into R with CytoML package)
umgcccfcsr.github.io
08 - Manual and Automated Gating – Cytometry in R
See Course tab for Course Materials, see YouTube tab for livestream recordings
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Libby Heeren @libbyheeren.bsky.social · 20/03/2026
Also, using the UI is the MOST SUCCESSFUL WAY I've ever been able to stash and pop changes. It's so niiiiice! Heres a fun game: count how many times I mention git making me cry (in the past) in this video 😂 #positron #databs #GitHub #Git #rstats #pydata
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Dirk Eddelbuettel @eddelbuettel.com · 01/03/2026
#r2u had its first 'three point five million packages delivered' month in Februaru (outside that two-day Sep 2023 outlier) bringing the total to over 68 million #CRAN #Rstats packages shipped as #Ubuntu .deb binaries. #r2u. Fast. Easy. Reliable. Pick All Three.
Dual-figure chart with #r2u cumulative donwload in mullion on the left, hitting 68 millions, and average weekly download (on a log scale) coming up on 100lk/week as we hit 3.49 million in the short month of February.
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Waggoner Lab @labwaggoner.bsky.social · 28/02/2026
FlowCLOc, a New Tool for Selecting the Most Appropriate Antibodies in Flow Cytometry www.mdpi.com/1422-0067/27...
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David Rach @davidrach.bsky.social · 21/02/2026
Hello #rstats folks, can anyone point me towards a good resource on how to set up/retrieve existing Google analytics account data and bring it into R? Not my usual #rstats niche, currently reading through the GoogleAnalyticsR vignette but post authentication not seeing any accounts or properties.
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Tyler, from the Internet (Haunted) @tylerfromtheinternet.com · 12/11/2025
Uh oh, you scrolled too far, too greedily, too deep: you have encountered a balrog.
Balrog roaring while being on fire. He's a big ol fire guy.
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Richard McElreath 🐈‍⬛ @rmcelreath.bsky.social · 02/02/2026
Wow this scoring chaos seems to be an extreme case of what I say about many ad hoc analyses: no derivation of method from a clear scientific theory, no assessment of statistical properties, and decades pass before someone notices. This happens in biology too, so let’s not pick on psychology only
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David Rach @davidrach.bsky.social · 27/01/2026
B
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David Rach @davidrach.bsky.social · 27/01/2026
That is all for now, we are excited to finally be getting started, astounded by the worldwide interest, glad that so many have chosen to join us, and looking forward to helping everyone get started on their own learning journeys. 7/7
media.tenor.com
a cartoon opossum is standing on a dirt road and saying `` thank you '' .
ALT: a cartoon opossum is standing on a dirt road and saying `` thank you '' .
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David Rach @davidrach.bsky.social · 27/01/2026
Feel free to share the course with anyone you feel may be interested ( #CytometryInR ). Everyone is welcome regardless of prior flow cytometry or coding experience. To be added to our mailing list, complete the original signup form here: docs.google.com/forms/d/e/1F... 6/7
docs.google.com
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David Rach @davidrach.bsky.social · 27/01/2026
Since everyone will need to get R and the other software installed on their computer and working correctly before the course starts up, we have made some pre-course walk-through tutorials available to hopefully make the process easier. These can be found at umgcccfcsr.github.io/CytometryInR... 5/7
umgcccfcsr.github.io
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David Rach @davidrach.bsky.social · 27/01/2026
Each week, all course materials for the upcoming week will be released on Sunday 2200 EST (Monday 0300 GMT+0) via our GitHub repository (github.com/UMGCCCFCSR/C... ). We will also be using it's Discussion page as a community forum for questions and to dive in more depth 4/7
github.com
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David Rach @davidrach.bsky.social · 27/01/2026
We will cover one topic for one hour each week. To accommodate different schedules, we will live-stream via YouTube (www.youtube.com/@CytometryInR) on Tuesday 2200 EST (Wednesday 0300 GMT+0), Wednesday 1600 EST (Wednesday 2100 GMT+0) and Thursday 1000 EST (Thursday 1500 GMT+0). Recordings? Yes! 3/7
youtube.com
UMGCCC FCSR
University of Maryland Greenebaum Comprehensive Cancer Center Flow Cytometry Shared Resource
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David Rach @davidrach.bsky.social · 27/01/2026
It has been in the works for quite a while, is focused around #flowcytometry data, and is the course that I wish had been available back in 2020 when I got thrown into the R deep-end. Schedule and course rationale can be found here: umgcccfcsr.github.io/CytometryInR... 2/7
umgcccfcsr.github.io
schedule – Cytometry in R
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David Rach @davidrach.bsky.social · 27/01/2026
Excited to officially announce our free "Cytometry in R" mini-course, aimed at coding beginners, will be starting up the first week of February! 🥳 It will be offered both online and in-person at the University of Maryland, Baltimore. 1500 people have signed up!!! #flowcytometry #rstats #quarto 1/7
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bioRxiv Immunology @biorxiv-immuno.bsky.social · 27/01/2026
Unmixing Spread Estimation Based on Residual Model in Spectral Flow Cytometry www.biorxiv.org/content/10.64898/20…
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Hadley Wickham @hadley.nz · 14/01/2026
(It also (optionally) plays a gong sound 🤣)
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Solomon Kurz @solomonkurz.bsky.social · 14/01/2026
The 0.5.0 version of my {brms} + {tidyverse} translation of McElreath's "Statistical Rethinking" (2nd ed) is up! solomon.quarto.pub/sr2/ 1/3 #rstats
solomon.quarto.pub
Statistical rethinking 2 with rstan and the tidyverse
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International Society for Advancement of Cytometry @isacytometry.bsky.social · 30/12/2025
At CYTO, it’s the people who make the experience unforgettable. Colleagues become friends, mentorships take shape, and a community comes together around a shared passion for cytometry.
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David Rach @davidrach.bsky.social · 16/12/2025
When anyone scans the codes, they get the following notice. This was about a week of being up. I will go open up an issue in case it is a third party API thing.
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David Rach @davidrach.bsky.social · 16/12/2025
Thanks Libby! Neither did I. Didn't find any obvious mentions in the documentation either after the fact, so kinda shocked given its on CRAN and no complaints about this aspect on the GitHub issues page.
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David Rach @davidrach.bsky.social · 16/12/2025
Thanks Sivuyile!
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International Society for Advancement of Cytometry @isacytometry.bsky.social · 15/12/2025
The CYTO 2026 abstract deadline is TODAY. This is your last chance to show off your research and join the global cytometry wave in West Palm Beach! Submission deadline: December 15th at 11:59pm ET. Submit today: www.cytoconference.org/abstracts.html
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David Rach @davidrach.bsky.social · 14/12/2025
Flow Cytometry Cores/Shared Resource Facilities at various institutions often accept external users, billing by hour. UofU's page: flowcytometry.cores.utah.edu (not familiar enough with y'alls area to know how far the other universities cores would be relative to SLC).
flowcytometry.cores.utah.edu
Welcome!! - University of Utah Flow Cytometry
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International Society for Advancement of Cytometry @isacytometry.bsky.social · 12/12/2025
Our English and Mandarin versions of our Basics of Flow Cytometry E-Course are now available free on YouTube! Check it out and make sure you like and subscribe to stay updated as we release additional educational content along with this course in additional languages! youtube.com/playlist?lis...
youtube.com
Basics of Flow Cytometry E-Course - YouTube
This playlist includes our Basics of Flow Cytometry E-Course in various languages. For more content like this, please visit our website at https://learning.i...
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VIB Technologies @vibtechnologies.bsky.social · 11/12/2025
Next May, the Computational Cytometry Summer School returns to Ghent for its 3rd edition. Sofie Van Gassen explains what participants can expect from this intensive hands-on program. tinyurl.com/msmzk74f Apply before 16 December 2025 ⚠️
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Rafael Irizarry @rafalab.bsky.social · 01/12/2025
📢 We are taking applications for our Postdoctoral Fellows Program at Harvard/DFCI! 🔹Join a research group in our department 🔹Co-mentoring opportunities with 2+ faculty 🔹Collaborate with investigators beyond our department 🔹Salary starts at $75K Apply here: t.co/B7SLZzQFKu
t.co
https://ds.dfci.harvard.edu/postdocs/
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Charlotte Hadley (she/her) @lottiemorecharlie.bsky.social · 01/12/2025
Merry December folks! Wanna know your most used and co-dependent geoms this past year?! I've built "Spotify Wrapped" for {ggplot2} that produces you an interactive story and analytical tools for your geom usage! #rstats Build your own report by following the docs: gpcds.com/ggplot2wrapp...
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David Rach @davidrach.bsky.social · 17/11/2025
Bought my laptop back in 2020, and #plannedobsolescence be dammed, I will use it until it disintegrates. Just swapped out the dying battery, and not worrying about finding the charger after 10 minutes unplugged is nice! Similar story for last years RAM upgrades and #Linux dual-boot.
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bioRxiv Immunology @biorxiv-immuno.bsky.social · 17/11/2025
Autofluorescence imaging reveals the impact of cryopreservation on T cell metabolism and activation response www.biorxiv.org/content/10.1101/202…
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Emily Young @eayoung.net · 15/11/2025
Maybe it’s a little over optimistic, but I legitimately feel like the fabled Year Of the Linux Desktop™ is really coming.
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Julia Silge @juliasilge.com · 08/11/2025
My talk from posit::conf earlier this fall on getting unstuck with #Python is now available to watch! - Ten years ago, I attempted to learn Python and it went EXTRMELY badly 😩 - I am really happy that so much tooling in this area has improved SO much since then 😌 youtu.be/pMVYl9fx1EE
youtu.be
How I got unstuck with Python (Julia Silge, Posit) | posit::conf(2025)
YouTube video by Posit PBC
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Emi @ Sekaicon B3 @emibirdee.art · 09/11/2025
Just restocked a lot of stickers including these guys! (shop.emibirdee.com/products/be-...)
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Jon Harmon (he/him/his) @jonthegeek.com · 08/11/2025
One more test from #RStats console before I share my wrapper around {bskyr}...
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James Holland Jones @juemos.bsky.social · 08/11/2025
This is an amazing (and infuriating) story, complete with Github repos of their analysis!
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