Peter Carlton @carltonlab.org · 3hI've long had a hunch that "able to do nondestructive photochemistry" is at least as big an influence as transmission through water; <300 does better than >600 but might destroy rather than isomerize a photoreceptor molecule. (data from Segelstein, D., 1981: "The Complex Refractive Index of Water") 010
Peter Carlton @carltonlab.org · 4hyes, but not that far into either the UV or IR. Too blue and you start destroying bonds; too red and you can't excite them. 121
Peter Carlton @carltonlab.org · 17hBingo! This is the primary reason, I think. Only that range can trigger nondestructive chemical changes in protein-coupled molecules. Same reason fluorescence microscopy uses a similar wavelength range for fluorophores. 1490
Peter Carlton @carltonlab.org · 02/10/2026If we ever do get to see the original NSWiM grayscale video, I expect it will look more or less like this. 110
Reposted by Peter CarltonMarie Held @makaefer.bsky.social · 28/09/2026Want to enhance your image analysis skills with napari? We’re running a two-day training event on how to use napari to effectively analyse your bioimaging data. 🕒 Applications close on 15 September. Learn more and apply 👇 www.crick.ac.uk/whats-on/cbi...crick.ac.uk 01216
Peter Carlton @carltonlab.org · 28/09/2026Not real data here in this example but I am really enjoying interactive #terminal plotting… 2 tmux panes with ssh replicating ~90% of what I usually do with jupyter. 010
Peter Carlton @carltonlab.org · 27/09/2026Interesting! It was 193 frames, 1288x720 px when I obtained it; shasum-1 : 1bccd…. I scanned the other 30 hosted entries and found no SynthID watermarks. 173
Reposted by Peter CarltonChristophe 🔬 Leterrier @christlet.bsky.social · 25/09/2026Here's a balanced account of where the microscopy community stands on AI use by this year's Small World in Motion winner. My main issue is that no one seems to have seen the original data despite requests (no mention of it being reviewed by the organizers) www.the-scientist.com/allegations-...the-scientist.comAllegations of AI Use in Nikon Competition Spark Controversy | The ScientistMicroscopists raise concerns about inappropriate AI usage in Nikon's microscopy contest-winning video. Nikon stands their ground, citing no policy breach. 67528
Peter Carlton @carltonlab.org · 25/09/2026Here is an amazing 1960's video from a company that once cared deeply about quality, starting with making their own glass www.youtube.com/watch?v=qwej...youtube.com科学の眼-ニコン-YouTube video by NPO法人科学映像館 010
Peter Carlton @carltonlab.org · 25/09/2026I know, and they also swing down into what should be intracellular space?! Here's another one using Grid/Collection stitching (thanks @preibischs.bsky.social !) showing the whole movie. 141
Peter Carlton @carltonlab.org · 24/09/2026Adventureland and the rest that came after: en.wikipedia.org/wiki/Adventu... My ideal and favorite game is Nethack - but these games flipped, as you say, the switch.en.wikipedia.orgAdventureland (video game) - Wikipedia 000
Peter Carlton @carltonlab.org · 24/09/2026Yes! I thought it might be because stage movement induced cilia bending, but if this is all in a sealed chamber it's unlikely that such an "inertial" effect would manifest (Life at Low Reynolds Number) -- but then again I don't know the relevant biomechanics of airway cilia well enough to judge. 101
Peter Carlton @carltonlab.org · 24/09/2026re:hallucination/fabrication: insofar as there's a difference, I think since they seem to have used one video as a basis and used AI to "enhance" it that it might be the better term? But I'm not really clear on the distinction myself. 000
Peter Carlton @carltonlab.org · 24/09/2026Diffraction ≠ refraction The authors' hallucinated contest entry and apparent misuse of AI in figure generation do not automatically imply their optical techniques are invalid; that has to be assessed separately. 100
Peter Carlton @carltonlab.org · 23/09/2026You may still need a microscope for raw images, may I suggest this model 120
Reposted by Peter CarltonSimon Cleary @simoncleary.bsky.social · 22/09/2026The response from Nikon makes me wonder whether tools marketed by Nikon were used: www.microscope.healthcare.nikon.com/en_EU/produc...microscope.healthcare.nikon.comNIS-Elements | NIS.aiThe optional NIS.ai module allows users to utilize pretrained AI networks for image processing, as well as create, annotate, and train custom AI networks for segmentation and analysis. Advanced image ... 262
Peter Carlton @carltonlab.org · 23/09/2026Yeah, they're firing all the lasers directly into the CCD! Or would be, if the beam splitter orientations were correct. Their earlier Nat.Comms paper had a more reasonable, less AI-flavored diagram. 080
Peter Carlton @carltonlab.org · 23/09/2026It wouldn’t even necessarily have to be their own tool; they all work more or less the same way and to disqualify the whole class might be politically bad within Nikon 020
Peter Carlton @carltonlab.org · 23/09/2026They state the video technically satisfies “AI generated videos are not permitted”, but unless they want a deluge of increasingly bizarre “merely AI-enhanced” videos in the future, they’d better provide clarity. 0102
Reposted by Peter CarltonChristophe 🔬 Leterrier @christlet.bsky.social · 22/09/2026From the new info my bet is fluo labeling inference pushed to 11. Hallucinating nonsensical DAPI nuclei, mitochondria stain, membrane labeling from a kind of phase contrast image: wrong localization, wrong scale, wrong movement. Keeping this movie as SWiM winner is a disgrace to the competition 69326
Reposted by Peter CarltonChristophe 🔬 Leterrier @christlet.bsky.social · 20/09/2026Human cilia disease specialist on the Small World in Motion winning movie 0156
Peter Carlton @carltonlab.org · 18/09/2026I have no idea about it, not being my area of expertise at all, but I am very curious about the methods used here. 010
Peter Carlton @carltonlab.org · 18/09/2026Registered from the beginning, caveats about compression etc apply of course 292
Reposted by Peter CarltonAndré Marques @amarques.bsky.social · 16/09/2026Out today in Nature: sex without crossovers. Rhynchospora tenuis makes pollen, fertilises and sets seed, yet 0 crossovers in 10,997 pollen nuclei. Chromosome drive and ~87% seed abortion rebuild the mother's genotype, so the offspring are clones. doi.org/10.1038/s415... 916372
Reposted by Peter CarltonShiori Iida @shiori-iida.bsky.social · 08/09/2026Happy to share that this paper is now published in Nature Genetics! Grateful to everyone who supported this work throughout this long journey. I’m proud to see it out! www.nature.com/articles/s41...nature.comCohesin prevents local mixing of condensed euchromatic domains in living human cells - Nature GeneticsSingle-nucleosome imaging/tracking and super-resolution three-dimensional structured illumination microscopy within euchromatic regions show that cohesin-mediated loops constrain condensed euchromatic... 22612
Reposted by Peter CarltonKazuhiro Maeshima @kazu-maeshima.bsky.social · 08/09/2026Is euchromatin really “open”? 🧬 Using super-resolution imaging🔬 our new study @natgenet.nature.com reveals: Euchromatin forms condensed domains in live cells. Cohesin constrains them and prevents domain mixing for proper transcriptional insulation🚧 🔗 www.nature.com/articles/s41... (1/2) 415067
Reposted by Peter CarltonMasa A. Shimazoe @masaashimazoe.bsky.social · 09/09/2026Our paper on euchromatin and cohesin is now published!!🙌 Euchromatin forms condensed domains, and cohesin constrains their local mixing. This organization maintains transcriptional insulation. This work was truly a team effort, and I’m grateful to everyone who contributed to it. 0205
Peter Carlton @carltonlab.org · 09/09/2026Back from an unforgettable trip to Indonesia! First, a weekend of discussing study opportunities with highly motivated students. Then, a few days waiting for a volcano to let us fly home. Glad to have spent 5 days in Jakarta, but have really just scratched the surface of an amazing city and country. 030
Peter Carlton @carltonlab.org · 08/09/2026Anak Krakatau has calmed down enough to let us slip out of Jakarta tonight; hopefully it will stay that way for a long time. 031
Reposted by Peter CarltonAnton Goloborodko @golobor.bsky.social · 03/09/20261/ out in @science.org! We found a new asymmetry in large-scale chromosome structure: sister chromatids are shifted by hundreds of kb in the 5′→3′ direction of their inherited strands! A close collaboration w/ @gerlichlab.bsky.social , led by @flaviacorsi.bsky.social www.science.org/doi/10.1126/... 215265
Peter Carlton @carltonlab.org · 03/09/2026He comes around here too, I hate that guy! But you just reminded me to put my 98% DEET in my travel bag since I'm headed to Indonesia for the weekend. 000
Peter Carlton @carltonlab.org · 03/09/2026Me too, but as a Crickian Orthodox Central Dogmatist I take issue with some phrasing here. 130
Peter Carlton @carltonlab.org · 03/09/2026modern terminals are amazing, it hasn't been this good since Tektronix emulation in xterm. 010
Peter Carlton @carltonlab.org · 29/08/2026I spent 16 years in the SF Bay Area, and have lived 16 more in Kansai, Japan where I'll most likely stay for the rest of my life, but this picture depicts one uniquely representative frame from my permanently imprinted birthplace (Seattle/Salish Sea) 040
Peter Carlton @carltonlab.org · 29/08/2026Make your favorite film sound as boring as possible without giving away the title: A family man from the country visits the big city, but when he is urged to participate in a religious ceremony, he goes back home. 000
Peter Carlton @carltonlab.org · 28/08/2026🇦🇺↔🇯🇵 for undergrad/masters students studying in Australia and interested in short-term research opportunities this summer (Jan 7 – Feb 18 2027) - please check out Kyoto University's KU-STAR program: www.kugd.k.kyoto-u.ac.jp/en/ku-star-p... ! Application deadline is Sep. 25, happy to take questions.kugd.k.kyoto-u.ac.jpKU-STAR Program AUSWhat’s the KU-STAR Program? KU-STAR Program, Kyoto University Short-Term Academic Research Pro 013
Peter Carlton @carltonlab.org · 16/08/2026I can never resist running full speed across them when there’s no one around. 000
Peter Carlton @carltonlab.org · 16/08/2026No, he could be Keith instead, or maybe Fox. Season 1: M&C Season 2: Post Captain/HMS Surprise Season 3: The Mauritius Command Season 4: Desolation Island Season 5: FoW/SM Season 6: Treason's Harbor,FSotW,RotM Season 7: LoM,13GS,NoC, bits of the rest 020
Reposted by Peter CarltonJoumana Medlej @majnouna.com · 13/08/2026Is Claude, let alone his pal Jimmy "Trust me bro" the Feckless, out there deciphering hundreds of pages of unconventionally-spelled scrawl and vanished words across a dozen text variants that require chemistry experiments to fully settle? 2245
Reposted by Peter CarltonMariko Sasaki @marikosasakiatnig.bsky.social · 06/08/2026The Sasaki lab at the National Institute of Genetics in Japan are seeking for highly motivated PhD students to work on extrachromosomal circular DNA, gene amplification, genome stability, and cancer genome dynamics, starting in October 2027. Please see below and apply by August 31. 043