Reposted by April Hayes
Very happy to share the publication of the first manuscript from my PhD! 🥳
Here, we show that PCR amplification of repetitive DNA sequences, such as AMR genes flanked by insertion sequences, can lead to the generation of artefact products that need to be interpreted cautiously.
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microbiologyresearch.org
PCR analysis of insertion sequences leads to the generation of artefact amplicons
Insertion sequences (ISs) are small, self-mobilizing DNA elements widespread across prokaryotic genomes, including chromosomes and plasmids. IS elements frequently co-localize with antimicrobial resis...