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Bini Ramachandran @itsbinir.bsky.social · 25/09/2026

A mundane question from a Friday evening brain.. If I have a protein overexpressing 20% more in a sample and it is the most abundant protein in that sample, how should I normalize the data for fold analysis for the rest of the proteins if an equal representative sample was analyzed.. #TeamMassSpec

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ypic.bsky.social @ypic.bsky.social · 22/09/2026

🔬 Ready to turn your proteomics idea into reality? The Student Proteomics Fund (SPF) is a student-focused grant designed to support students in bringing their proteomics experiments to life! 📅 Call opens: October 1st, 2026 ⏰ Submission deadline: November 30th, 2026

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Bini Ramachandran @itsbinir.bsky.social · 20/08/2026

That's a frigging peptide spectrum match.. Playing with endogenous peptides on mass spec is funny.. 😊😊

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Bini Ramachandran @itsbinir.bsky.social · 18/08/2026

Hello #Astral users, Have you ever come across the ion foil adjustment failure error?? We have started seeing it since the tune was upgraded to 2.2 recently during the PM. The error interferes with the system getting ready to run a sample in the step where astral mass accuracy is checked with ICS.

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IonOpticks @ionopticks.com · 11/08/2026

One of the most overlooked factors in maintaining consistent, high-quality spray in nano-scale liquid chromatography is something you handle every time you connect your column: your capillary fitting. Thankfully, a little care & a few simple habits go a long way. Here's what to know: bit.ly/4zbjHd4

bit.ly

Caring for your capillary fitting: small habits for stable spray

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Bini Ramachandran @itsbinir.bsky.social · 21/07/2026

A question to mass spectrometrists in the core facilities around the UK and Europe.. How are you finding the cost of S-traps for proteomics applications?? It looks like incorporation of S-traps in the workflow adds an additional £8 per sample.. It feels extraordinarily expensive..

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ASMS @asms.org · 25/06/2026

Hey, #TeamMassSpec! Interested in a career in academia? #ASMS will host 2 free Zoom panel discussions ft. college faculty sharing insights on preparing your academic job application package and what to expect during the interview process. Register now! bit.ly/4c1VrR5

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Bini Ramachandran @itsbinir.bsky.social · 23/06/2026

Never been a fan of the so-called number game.. However, it is indeed exciting when you get to see 100 precursors identified per second from biological samples, not just from a std digest.. Pretty stoked by having a front raw seat to witness the technology evolution through ages..😊😊 #MassSpecMusings

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EMBL @embl.org · 22/06/2026

The EMBL #ARISE2 Fellowship Programme, funded by MSCA, opens for applications 30 June. This unique opportunity trains researchers to lead #ResearchInfrastructures & innovate in #LifeSciences. 🧪 🗓️ Join our webinar on Thursday, 2 July at 09:30 CET: embl-org.zoom.us/webinar/regi... #careers

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Bini Ramachandran @itsbinir.bsky.social · 20/06/2026

#TeamMassSpec, may I please check if there is a lab where Thermo #Ardia is seamlessly connected to multiple mass specs and process proteomics data processing and management on the server itself?? If yes, can I please have a brief chat?? I am interested in hearing more about it.. Thanks very much..

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Bini Ramachandran @itsbinir.bsky.social · 17/06/2026

What is the magic soup in the PreOmics iST kit, I wonder.. It is getting me 6000 proteins from tiny FFPE samples!! Whereas the conventional workflow is hardly touching 1000 proteins!!

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Roland Pease @peaseroland.bsky.social · 16/06/2026

Another UK chemistry department in trouble - this time Sheffield. savesheffieldchemistry.netlify.app

savesheffieldchemistry.netlify.app

An Open Letter

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Bini Ramachandran @itsbinir.bsky.social · 11/06/2026

Is there any option to remove the contaminant proteins prior to normalisation in #Spectronaut?? Essentially, I would like to identify them, but don't want to include their abundance values for cross run normalisation.. is there a possibility?? Thanks very much..

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Bini Ramachandran @itsbinir.bsky.social · 01/04/2026

Hello #Spectronaut users and manufacturers.. Is there any option to export both the raw and normalized PG.Quantity values?? Is it not possible at all or am I missing something here?? Thanks very much..

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PastelBio @pastelbio.bsky.social · 19/03/2026

A nature-inspired ion trap for parallel manipulation of ions on a massive scale www.science.org/doi/... --- #proteomics #prot-paper

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Franck Pichaud @fpichaud.bsky.social · 16/03/2026

Wow! www.nature.com/articles/s41... Here we report all five canonical nucleobases—purines (adenine and guanine) and pyrimidines (cytosine, thymine and uracil)—in samples returned from the C-type asteroid (162173) Ryugu

nature.com

A complete set of canonical nucleobases in the carbonaceous asteroid (162173) Ryugu - Nature Astronomy

Samples returned from the asteroid Ryugu contain all five canonical nucleobases (A, G, C, T and U). Their presence in Ryugu and Bennu supports the hypothesis that carbonaceous asteroids contributed to...

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Bini Ramachandran @itsbinir.bsky.social · 11/03/2026

Setting up a decent DDA method on Astral is so mentally taxing.. It isn't meant to follow any of the data dependency criteria.. Enforcing it to follow the criteria is where it gets taxing.. Wonder how it is working for those MS2-TMT workflows.. 🤔🤔

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Bini Ramachandran @itsbinir.bsky.social · 08/03/2026

It is an incredible honour to be featured in the Faces of Mass Spectrometry series of JASMS and share the story of a nerdy mass spectrometrist.. who despite being an introvert, always love to talk about how wonderful mass spectrometry is.. ☺️☺️ Proud to be a part of #TeamMassSpec and #FeMS.. Thanks..

pubs.acs.org

Faces of Mass Spectrometry/Bini Ramachandran

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Bini Ramachandran @itsbinir.bsky.social · 03/03/2026

Today at 13:50, on the dot, the sequences running on both Ascend and Eclipse came to an abrupt stop.. Just at random.. In the middle of a run.. Neo kept running but no MS acquisition.. Had to restart Xcalibur and reconnect LC to resume the sequence.. Very spooky!!! #MassSpecMusings

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Bini Ramachandran @itsbinir.bsky.social · 02/03/2026

Anybody doing proteomics on FACS sorted cells? People are using BSA coated tubes to avoid cell adhesion onto the walls. BSA is all over the protein extract. How to avoid this BSA? Saw Polyvinyl alcohol (PVA) can be used in place of BSA. Any experience with its compatibility for downstream MS? Thanks

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Bini Ramachandran @itsbinir.bsky.social · 20/02/2026

"This work provides insight to help future proteomics study designers make more informed decisions on whether physical normalization is necessary for their experiments" Guess I am that old rigid mass spectrometrist now who finds it hard to keep an open mind to new concepts.. 😉😉

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Bini Ramachandran @itsbinir.bsky.social · 16/02/2026

I have all these glycan specific fragments from a glycopeptide analysis.. Any software suggestions for identifying both the peptide and the attached glycan composition from such spectra?? Thanks very much.. #glycotime #TeamMassSpec

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Bini Ramachandran @itsbinir.bsky.social · 03/02/2026

#TeamMassSpec Question for XL-MS practitioners.. Looking for a suitable crosslinker to stabilise a protein complex in vitro.. What are the pros and cons for MS-cleavable vs non-cleavable cross-linkers?? Is PhoX a good choice?? Any recommendations for MS-cleavable cross-linkers?? Thanks very much..

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VIB Technologies @vibtechnologies.bsky.social · 26/01/2026

The Spatial Omics Summer School offers a structured, hands-on introduction to spatially resolved targeted transcriptomics, proteomics, and metabolomics. If you want to join, submit your application by 2 February 2026. www.vibconferences.be/events/spatia…

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Kermit Murray @kkmurray.bsky.social · 19/01/2026

(J Proteom) Two-dimensional electrophoresis-based proteomics reveals soybean seed hypocotyl proteoforms: Publication date: Available online 17 January 2026 Source: Journal of Proteomics Author(s): Jian-Zhong Tan, Hiroyuki Kagawa, Keiko Kizawa, Hisashi Hirano #MassSpecRSS

dlvr.it

Two-dimensional electrophoresis-based proteomics reveals soybean seed hypocotyl proteoforms

Publication date: Available online 17 January 2026 Source: Journal of Proteomics Author(s): Jian-Zhong Tan, Hiroyuki Kagawa, Keiko Kizawa, Hisashi Hirano

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Kermit Murray @kkmurray.bsky.social · 10/01/2026

(JASMS) [ASAP] Faces of Mass Spectrometry/Khatereh Motamedchaboki: Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00430 (RSS) #MassSpecRSS #JASMS

dlvr.it

[ASAP] Faces of Mass Spectrometry/Khatereh Motamedchaboki

Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00430

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Bini Ramachandran @itsbinir.bsky.social · 07/01/2026

Vanquish Duo as Neo Mono with EasySpray column setup.. Works quite alright.. Guess have to get uPAC to test the dual column setup at nano flow rate..

Chromatogram reproducibility for HeLa QC

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Bini Ramachandran @itsbinir.bsky.social · 26/12/2025

A slide from a presentation I gave in 2013 on mass spectrometry proteomics.. Ironically, still a relevant topic to include this slide as such in a talk I am preparing to give after the new year..

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Bini Ramachandran @itsbinir.bsky.social · 23/12/2025

Holidays started.. And the festive decor is on.. 🎉🎉#MassSpecHolidayDecor

Column emitter with a weird thing stuck on..

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Duncan Bell @duncanbell.bsky.social · 19/12/2025

Post-doc positions: "Academic freedom is under pressure today. This requires rescue havens of free research. ... [we] invite early career researchers, whose work is restricted due to political pressure in the USA..." uni-freiburg.de/frias/call-f...

uni-freiburg.de

Call for Applications: Early Career Rescue Fellowship – Freiburg Institute for Advanced Studies

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Bini Ramachandran @itsbinir.bsky.social · 19/12/2025

Guess at which point I realized that the column heater is not connected.. I am very glad that this is the only boss-back-on-bench catastrophe so far.. The muscle memory from years of proteomics works still.. Thankfully, nothing got blown up.. yet.. 😂😂

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Bini Ramachandran @itsbinir.bsky.social · 18/12/2025

Damn.. Neo firmware update 2.51 locks you out of the autosampler and prevents you from opening the door once the sequence is started.. Damn..

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Bini Ramachandran @itsbinir.bsky.social · 16/12/2025

#TeamMassSpec Can someone please help me remember whether the Vanquish Duo launched at ASMS2024 was meant for nanoLC applications?? Somehow, I remember it as the potential high speed solution from Thermo for Astral high SPD applications.. However, the Duo I got hold of here is a microflow version!!

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Bini Ramachandran @itsbinir.bsky.social · 15/12/2025

TMT-MS2 Quan with 0.6Th ultra narrow window DIA.. 🤯

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PastelBio @pastelbio.bsky.social · 08/12/2025

Proteomics Specialist - DTU Biosustain | JobTeaser www.jobteaser.com/fr... --- #proteomics #prot-jobs

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US HUPO @us-hupo.org · 01/12/2025

We are thrilled to announce our 2026 election results. Olga Vitek has been elected to serve as US HUPO's President-Elect, Devin Schweppe as Secretary, and Sasha Singh and Hanno Steen were elected to serve as Members-At-Large on our Board of Directors. Congratulations to all! 🌟 #USHUPO

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Bini Ramachandran @itsbinir.bsky.social · 26/11/2025

#Glyco people?? Just like PNGase F, is there a glycosidase that can cleave off O-glycan from S/T and leave a mass signature when the reaction is carried out in heavy water?? Thanks very much..

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Bini Ramachandran @itsbinir.bsky.social · 21/11/2025

Even when the 96% of study sample contributors were females, the 4% males are the ones who get the mention!!! Wonder why can't the demography of the sample population be mentioned as 96% female or for better clarity as 96:4 female:male in the abstract.. Another instance of scientific gender bias??

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Luke Gamon @lgamon.bsky.social · 20/11/2025

Quick heads up - there will soon be a job posting for a Staff Scientist / Senior Staff Scientist in our lab in Copenhagen to support all our mass spec proteomics adventures! Great colleagues, lots of cool projects… and you’ll get to work with me 😅 Please DM if you’re interested!

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Females in Mass Spectrometry (FeMS+) @femalesinms.bsky.social · 11/11/2025

Registration for the Round 6 of Mentorship Pods is now OPEN! More info👉 femalesinms.com/mentorship-p... Registration 👉 femalesinms.com/mentorship-p...

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PastelBio @pastelbio.bsky.social · 04/11/2025

Changes to UniProt proteomes www.ebi.ac.uk/about/... --- #proteomics #prot-other

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Bini Ramachandran @itsbinir.bsky.social · 31/10/2025

Hello #Proteomics folks from core labs, Have you all moved over to IonOptiks columns?? Am I the only one left?? I know these columns give awesome chromatograms. How is the longevity of these columns in core labs??? May I ask what's your best and worst column life stats?? Thanks very much..

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Females in Mass Spectrometry (FeMS+) @femalesinms.bsky.social · 30/10/2025

📢 FeMS Q4 2025 Empowerment Award applications are now open! More info here 👉https://femalesinms.com/empowerment/ Apply here👉https://airtable.com/applvoZXoHURMcSCq/shrQlJoZ6f2P5yWqc #FeMS #TeamMassSpec #WomenEmpowerment

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Females in Mass Spectrometry (FeMS+) @femalesinms.bsky.social · 30/10/2025

🌟 We are excited to announce that applications are now open for the Rising Star in Computational Mass Spectrometry Award! 🌟 Go to: lnkd.in/gzDuppnb to see the details and to apply! Thanks to our sponsors @chanzuckerberg.bsky.social and Open MS!

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John Yates @jyates.bsky.social · 28/10/2025

Hey @asms.org peeps. I’m running for VP for programs and I’d like your vote. Check out the asms web site for details and you will see an email with voting instructions in the near future. I approach this position with lots of experience organizing scientific conferences & I ❤️ asms. Please re-sky.

media.tenor.com

a spongebob squarepants scene with a mailbox and a flower

ALT: a spongebob squarepants scene with a mailbox and a flower

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Brett Phinney @ucdproteomics.bsky.social · 27/10/2025

Need to do some cutting edge Proteomics Differential expression? Maybe give Limpa a try! Guaranteed to rise to the occasion ...despite its name ! youtu.be/vqeOT8xcycc

youtu.be

Doing Differential Expression analysis on Proteomics Data using Limpa!!

YouTube video by Brett Phinney

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British Society for Proteome Research @ukbspr.bsky.social · 26/10/2025

We are delighted to inform you that BSPR 2026 will be held at the @ebi.embl.org campus in Cambridge from the 13th to the 15th of July. Make sure to save the data and join us for the a meeting full of great science focused on British & Irish Proteomics. #proteomics #BSPR2026 #massspectrometry

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Bini Ramachandran @itsbinir.bsky.social · 16/10/2025

Would you do tissue proteomics with an old sample fixed in formalin, not embedded in paraffin, and stored for quite a while immersed in formalin?? Can the proteins be extracted after subjecting to decrosslinking?? Are there any insights to share from the #paleoproteomics practitioners?? Thanks..

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