iiinova — where your digital self keeps evolving

Anita Donlic @anitadonlic.bsky.social · 16/09/2026

🔬 Staff Scientist Looking for someone with strong mammalian cell culture + microscopy experience who is excited about extracting biological insight from imaging data. Programming/image analysis experience important; ML/computer vision a big plus! Apply: wustl.wd1.myworkdayjobs.com/External/job...

wustl.wd1.myworkdayjobs.com

Staff Scientist - Biochemistry

Location ST. LOUIS, MO 63110 Scheduled Hours 40 Position Summary The candidate is expected to independently design, conduct, and analyze mammalian cell culture and quantitative fluorescence microscopy...

000

Anita Donlic @anitadonlic.bsky.social · 16/09/2026

🧬 Research Technician Looking for someone interested in molecular biology/biochemistry to work on cloning, PCR, nucleic acid purification, in vitro transcription, gels & more. Mammalian cell culture experience is a plus, but not required. Apply: wustl.wd1.myworkdayjobs.com/External/job...

wustl.wd1.myworkdayjobs.com

Research Technician II - Biochemistry

Location ST. LOUIS, MO 63110 Scheduled Hours 40 Position Summary Assists with the technical aspects of studies and experiments, including documentation and preparation of materials. Will also support ...

120

Anita Donlic @anitadonlic.bsky.social · 16/09/2026

We have openings for a Research Technician + Staff Scientist! 👇Please help spread the word or share with someone who might be a great fit. Both positions are a chance to join at the very beginning and help shape the science and culture of a brand-new lab. 🧪✨ Please apply via the links below 🔗.

100

Anita Donlic @anitadonlic.bsky.social · 16/09/2026

🎉 I'm thrilled to announce that the Ðonlić Lab has officially opened this month in the BMB department @wusm-bmb.bsky.social at @washumedicine.bsky.social! The lab will study how condensates shape RNA interactions, therapeutic activity, and disease progression. And - we're hiring! 👇

donliclab.com

Đonlić Lab

Decoding cellular biochemistry from microscopy: chemical tools, RNA biophysics, and machine learning in condensates.

1175

Reposted by Anita Donlic

Jerelle Joseph @jerelleaj.bsky.social · 20/08/2026

Out now in #JACS work led by the talented @nathanieldhess.bsky.social on protein folding inside condensates!! pubs.acs.org/jacsat/artic...

12913

Anita Donlic @anitadonlic.bsky.social · 05/06/2026

Super grateful to the amazing dream team from @brangwynnelab.bsky.social and @aiinglim.bsky.social labs that made this work possible!

000

Anita Donlic @anitadonlic.bsky.social · 05/06/2026

Thrilled to see our Deep-Phase paper now online in @cellcellpress.bsky.social! 🎉 The final version expands Deep-Phase across labeling modalities, adds dual-condensate imaging to dissect pathway specificity, and shows greater robustness to batch effects than classical image-analysis methods.

cell.com

Deep learning of functional perturbations from condensate morphology

A deep learning method quantitatively decodes nucleolar, nuclear speckle, and viral condensate morphology to reveal underlying biochemical states. Applying this framework reveals a previously unrecogn...

1116

Reposted by Anita Donlic

Jordy F. Botello @jbotello.bsky.social · 10/03/2026

Some molecular machines, like ribosomes, can persist for long periods of time in cells. Could molecular aging of ribosomes shape how proteins are made? In our new preprint we track ribosomes as they age in cells and uncover unexpected effects on translation (1/10) www.biorxiv.org/content/10.6...

412851

Reposted by Anita Donlic

Amy Strom @dnamystrom.bsky.social · 22/01/2026

I'm hiring a summer intern! Pursuing a PhD and interested in industry experience? Apply at the link below by Jan 26 to spend 12 weeks onsite at South San Francisco with Genentech's Discovery Oncology department in May-July 2026 pursuing novel therapies targeting disordered protein regions.

roche.wd3.myworkdayjobs.com

2026 Summer Intern - Discovery Oncology

2026 Summer Intern - Discovery Oncology Department Summary The Department of Discovery Oncology is seeking a talented and motivated summer intern to join the biology research efforts to identify and c...

131

Anita Donlic @anitadonlic.bsky.social · 29/09/2025

Congratulations!! This is so exciting and well-deserved. 🎉

120

Anita Donlic @anitadonlic.bsky.social · 20/08/2025

... Prof. Ai Ing Lim and @kristantunes.bsky.social for a great help with the virus work, and Cliff @brangwynnelab.bsky.social for all the support and guidance!

020

Anita Donlic @anitadonlic.bsky.social · 20/08/2025

To say that this took a village would be an understatement. I’m incredibly grateful to my collaborators in the @brangwynnelab.bsky.social : Troy Comi, @sofiquinodoz.bsky.social, @nima-jaberi.bsky.social, Lenny Weisner and Lifei Jiang, ...

110

Anita Donlic @anitadonlic.bsky.social · 20/08/2025

🌟 Big picture 🌟 ✔️ Condensate morphology encodes function ✔️ Deep-Phase translates images into quantitative readouts of drug biochemical potency ✔️ Future applications: phenotypic screens, MoA studies, biomarkers, single-cell heterogeneity detection (10/10)

110

Anita Donlic @anitadonlic.bsky.social · 20/08/2025

Integrating our discoveries, we came up with a new model for nucleolar phase boundary maintenance, in which both the abundance and processing state of rRNA📊 dictate the multiphase arrangement within this fascinating condensate (9/10).

120

Anita Donlic @anitadonlic.bsky.social · 20/08/2025

These observations led us to hypothesize that sequential cleavage inhibition💊 (invert & push apart DFCs from GC) followed by transcription inhibition💊 (restore DFC-GC interface) could phenocopy the flower 🌸 morphology. This is indeed what we (and Deep-Phase) saw! (8/10)

110

Anita Donlic @anitadonlic.bsky.social · 20/08/2025

We then conduct mechanistic studies 🔎 to find that beyond its recognized role in resolving supercoiling, TOP1 is also involved in rRNA processing! Its inhibition causes rRNA cleavage defects and a drop in transcription of large ribosomal subunit precursors ❌🏭. (7/10)

120

Anita Donlic @anitadonlic.bsky.social · 20/08/2025

To test the utility of Deep-Phase, we conduct a small molecule screen and uncover a unique nucleolar morphology: the "flower" 🌸! This phenotype, where the DFC-GC interface is maintained but inverted, arises specifically upon DNA Topoisomerase I (TOP1) depletion. (6/10)

110

Anita Donlic @anitadonlic.bsky.social · 20/08/2025

Not just nucleoli! We extend Deep-Phase to nuclear splicing speckles✂️ as well as RSV viral cytoplasmic factories🦠, showing that condensate morphology alterations are a quantitative fingerprint of the degree of RNA biochemistry disruptions within them. (5/10)

110

Anita Donlic @anitadonlic.bsky.social · 20/08/2025

In doing so, we measure dose-response curves from images and demonstrate that they match IC50 values from biochemical experiments: nucleolar morphology becomes a quantitative readout of potencies of drugs that alter ribosome biogenesis! 📉➡️📈 (4/10)

110

Anita Donlic @anitadonlic.bsky.social · 20/08/2025

We developed Deep-Phase, an automated imaging and deep neural network-based framework that accurately tracks these changes over treatment times⏲️and concentrations📶 directly from images (no hand-picked features) (3/10).

110

Anita Donlic @anitadonlic.bsky.social · 20/08/2025

Cells organize key processes in biomolecular condensates, such as the multiphase nucleolus where ribosomes are made 🏭. Due to its dynamic and compartmentalized nature, small molecule perturbations of this process💊 lead to fast and distinct morphology rearrangements🌀. (2/10)

120

Anita Donlic @anitadonlic.bsky.social · 20/08/2025

I’m thrilled to share our preprint that uses deep learning to interrogate structure-function relationships in condensates! biorxiv.org/content/10.1... In here, we ask: can AI read condensate biology from pictures alone? Turns out yes... see what we discover below! 🤖🧬🖼️ (1/10)

biorxiv.org

Deep Learning of Functional Perturbations from Condensate Morphology

Biomolecular condensates compartmentalize the interior of living cells to spatiotemporally organize complex functions, yet linking molecular interactions within condensates to their mesoscale organiza...

2133

Anita Donlic @anitadonlic.bsky.social · 20/08/2025

Ai Ing Lim and @kristantunes.bsky.social for a great help with the virus work, and Cliff @brangwynnelab.bsky.social for all the support and guidance!

010

Anita Donlic @anitadonlic.bsky.social · 20/08/2025

100

Anita Donlic @anitadonlic.bsky.social · 20/08/2025

100

Anita Donlic @anitadonlic.bsky.social · 20/08/2025

110

Anita Donlic @anitadonlic.bsky.social · 20/08/2025

100

Anita Donlic @anitadonlic.bsky.social · 20/08/2025

110

Anita Donlic @anitadonlic.bsky.social · 20/08/2025

To test the utility of Deep-Phase, we conduct a small molecule screen to uncover a unique nucleolar morphology: the "flower" 🌸! This phenotype, where the DFC-GC interface is maintained but inverted, arises specifically upon DNA Topoisomerase I (TOP1) depletion. (6/10)

100

Anita Donlic @anitadonlic.bsky.social · 20/08/2025

100

Anita Donlic @anitadonlic.bsky.social · 20/08/2025

100

Anita Donlic @anitadonlic.bsky.social · 20/08/2025

We developed Deep-Phase, an automated imaging and deep neural network-based framework that accurately tracks these changes over treatment times⏲️ and concentrations📶 directly from images (no hand-picked features).(3/10)

100

Anita Donlic @anitadonlic.bsky.social · 20/08/2025

181