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Emma R Andersson

@anderssonlab.bsky.social
629 followers 428 following 85 posts

Assoc Prof at Karolinska Institutet. Dev bio enthusiast, from liver to brain, and Notch to miRs, cell fates and lineages.. www.anderssonlab.com

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Emma R Andersson @anderssonlab.bsky.social · 24/09/2026
Thanks!! I would absolutely love to implement in utero genetic manipulation in striped mice with you! We can have a lot of fun together and answer so many questions 🤩🤓
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Emma R Andersson @anderssonlab.bsky.social · 24/09/2026
Thank you! 🤩 really excited about the doors this opens!
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Emma R Andersson @anderssonlab.bsky.social · 24/09/2026
Thank you!
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
www.biorxiv.org/content/10.6...
biorxiv.org
In utero barcoding of mouse mesoderm reveals the clonal architecture of organ mesenchyme
The mesoderm coordinates organogenesis and generates the cardiovascular, visceral and musculoskeletal systems, yet in mammals this germ layer is difficult to access, impeding its genetic manipulation ...
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
Would love that! Big fan of your 2020 LPM review (it’s the first citation in the manuscript 😅👏), and lots of your heart work! Happy to zoom - you can email me: Emma.andersson at ki.se
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
Ooh and addendum: in sections, we can see labelling of notochord. It’s not in the manuscript (out of scope), and we couldn’t identify them in the scRNAseq (too few cells?) but it seems ExC injection can even access notochord, though maybe not 100%. We did not try to optimize this further for now.
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
Thank you so much! 🤩🙏
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
I agree with you - lateral plate mesoderm is the most obviously accessible to this route and is very well targeted. But we also see targeting of paraxial and intermediate mesoderm populations (in Fig 2) - following up on this now separately 🤓
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
Can I replace impact factor with a gif-rating scale, implemented by you? 🔥
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
Thanks!!! You have a way with words 🤩
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
Many thanks also to the funders: ERC @erc.europa.eu, Knut and Alice Wallenberg Foundation, Swedish research Council @vetenskapsradet.bsky.social, Leo Foundation, Hjärnfonden, the Göran Gustafsson Prize in Medicine, Karolinska Institutet and the Wellcome Trust.
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
(cont) Jia Sun, Bettina Semsch, Noemi Van Hul, Robert Tonoian @rob-ton.bsky.social, John Wilson-Kanamori, Ulrika Marklund @ulrikakmarklund.bsky.social, Marcel Martin, Neil Henderson, Jonas Frisén, Michael Ratz, Peter Kharchenko, Igor Adameyko @adameykolab.bsky.social , Enikő Lázár &Joakim Lundeberg!
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
An enormous effort led by Jingyan He, and Johan Lorentz. Very grateful to excellent lab members and external collaborators also contributing to this project: Agustín Andrés Corbat, Sergey Isaev, Raphaël Mauron, Sandra de Haan @sandehaan.bsky.social, Lenka Belicova @lenkabelicova.bsky.social, (cont)
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
Several long-standing controversies in cardiac and hepatic mesenchyme trace directly to this limitation. In utero barcoding resolves ancestry without first choosing a marker, and opens a previously inaccessible germ layer to rapid genetic manipulation. 🤩
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
This is why bypassing Cre drivers matters. Enhancer/promoter-based drivers report marker expression, not ancestry. In organ mesenchyme, transient progenitors share markers, while distinct lineages can converge on similar transcriptional states.
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
Most strikingly, transcriptionally similar valve-mesenchymal cells can have fundamentally different origins: epicardial/mesodermal or neural crest. (disclaimer, endocardium was not targeted with our approach) So, cell state (convergent mesenchyme) does not reveal developmental history.
Three Visium HD spatial deconvolution maps of an E16.5 mouse heart. The left panel shows neural crest-related valve-mesenchymal populations identified in our study and great-artery smooth muscle cells concentrated around the aorta and aortic valve, with little contribution to the mitral valve. The middle panel maps epicardium, epicardium-derived cells and intermediate populations identified as clonally related, along a spatial route from the heart surface toward the valves. The right panel shows more differentiated mesoderm-related valve populations concentrated within the developing valve region.
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
Read the preprint to find out what all the cardiomyocyte and mesenchymal subtypes are, and where they are spatially located 😜
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
Meanwhile, the epicardium generates three spatially organized cardiac mesenchymal trajectories.. but shows no substantial clonal contribution to cardiomyocytes. Marker-independent ancestry thus helps address a long-standing developmental controversy.
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
In heart, atrial and ventricular cardiomyocyte lineages are strongly segregated by E7.5. Yet left–right ventricular identities remain embedded within a more plastic continuum of ventricular competence.
Hierarchically clustered heatmap of clonal-coupling correlations among fetal heart cell populations. Red indicates positive and blue negative correlation. The analysis separates ventricular cardiomyocyte clones from epicardium-related mesenchymal clones, with strong positive correlations within each compartment and negative correlations between them. Epicardium-related cells form three distinct clonal lineages, while atrial cardiomyocytes (aCM) and several valve, vascular and non-cardiac populations form separate branches.
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
We next barcoded mesoderm and analyzed heart development with single cell lineage tracing and VisiumHD to again annotate all the new cell types with high confidence.
Experimental workflow for fetal heart lineage tracing. At E7.5, a lentiviral barcode library was injected into the exocoelomic cavity. At E16.5, four barcoded hearts were collected and tdTomato-positive, barcode-positive cells were isolated for 10x Chromium single-cell RNA sequencing, alongside two non-injected hearts. Visium HD spatial transcriptomics of two control hearts enabled spatially informed cell annotation, followed by barcode-based clonal analyses.
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
(Note: LOTS of hematopoietic cells in the fetal liver, as expected, also lineage traced and with further exciting work to come here!)
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
In liver, mesothelium is not a uniform progenitor pool. Spatially distinct mesothelial subsets are already lineage-biased toward stellate cells versus fibroblast and mural-cell fates - revealing multiple routes to liver mesenchymal diversity.
Hierarchically clustered heatmap of clonal-coupling correlations among fetal liver cell populations. Red indicates positive and blue negative correlation. Distinct modules separate mesothelial-related populations, including hepatic stellate cells, from fibroblast/mural populations and from hepatoblast, endothelial, erythroid and other blood lineages. The strongest within-module correlations reveal lineage-biased relationships among liver mesenchymal populations.
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
So we barcoded mesoderm at E7.5, and analyzed liver development at E16.5 with single cell lineage tracing and VisiumHD to annotate the many new cell types/states in a spatially aware manner, with high confidence.
Experimental workflow for fetal liver lineage tracing. At E7.5, a lentiviral barcode library was injected into the exocoelomic cavity. At E16.5, four barcoded livers were collected and tdTomato-positive, barcode-positive cells were isolated for 10x Chromium single-cell RNA sequencing, alongside three non-injected livers. Visium HD spatial transcriptomics of two control livers enabled spatially informed cell annotation, followed by barcode-based clonal analyses.
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
Since the technology labelled mesoderm, and in particular septum transversum mesenchyme, we wanted to investigate its possible derivative lineages, including liver mesothelium and heart epicardial lineages.
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
The scale: >590,000 cells and 31,000 multicellular clones, followed from gastrulation into fetal heart and liver. By combining clonal ancestry, cell state and spatial position, we uncovered developmental histories invisible to transcriptomics alone.
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
That old image revealed an opportunity hiding in plain sight: an accessible route to developing mesoderm. We used it to deliver lentiviral reporters and barcodes at E7.5: a scalable, Cre-independent method to manipulate and lineage-trace mammalian mesoderm in utero.
Two UMAP plots of 242,486 single cells from whole E9.5 and E10.5 mouse embryos. The left plot identifies 18 populations spanning ectodermal, neural crest, mesodermal, endothelial, hematopoietic and epithelial lineages. The right plot shows tdTomato-positive cells following E7.5 exocoelomic-cavity injection, comprising 92.8% of recovered cells and strongly enriching mesodermal populations, particularly limb, pharyngeal-arch and splanchnic lateral-plate mesoderm derivatives. Colored cells represent 6,312 E9.5 cells from 10 embryos and 55,830 E10.5 cells from five embryos.
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
Sometimes a new technology begins with a 60-year-old anatomical drawing. A 1966 image from Biology of the Laboratory Mouse showed a crucial detail: the extraembryonic exocoelomic cavity is continuous with the embryo’s coelomic cavity.
Figure 12-18, Snell and Stevens, Biology of the Laboratory Mouse, 2nd ed., 1966; digitized by Mouse Genome Informatics, The Jackson Laboratory. Black and white drawing of cross-section of E8 mouse embryo.
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Emma R Andersson @anderssonlab.bsky.social · 23/09/2026
🧬 Preprint! 🧵 Our goal is to genetically manipulate every embryonic germ layer to enable high throughput in vivo studies. We previously showed mouse ectoderm can be targeted by amniotic injection, enabling cell-specific manipulation, lineage tracing, and knockouts. But how to reach mesoderm?
Schematic of method developed and application. At embryonic day 7.5, a lentiviral library carrying unique 30-nucleotide barcodes and a fluorescent reporter is injected into the mouse exocoelomic cavity. An E8 embryo cross-section shows that this cavity is continuous with the intraembryonic coelomic cavity, allowing the virus to reach and barcode mesodermal progenitors, including lateral plate and paraxial mesoderm.
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Olivia Waite @oliviawaite.com · 22/09/2026
Oh my god it’s funny because it’s true — take a gander at the video and then read up: en.wikipedia.org/wiki/Dunnes_...
en.wikipedia.org
Dunnes Stores strike - Wikipedia
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POLITICO Europe @politico.eu · 03/08/2026
Andy Burnham has promised to transform Britain. But can he afford it? 🎙️ On #PASAA, we sit down with Sky News’ economics editor Ed Conway to examine the spending choices facing the new PM. 🎧 Listen:
podcasts.apple.com
What will Burnham do with the Budget?
Andy Burnham has promised to transform Britain, but can he afford it? This autumn, Chancellor John Healey will deliver the new government's first Budget, so wha
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Gonçalo Castelo-Branco @gocastelobranco.bsky.social · 24/06/2026
Join us at the 13th Developing Brains, October 7th, Nobel Forum @ki.se! Register at news.ki.se/calendar/dev... with @anderssonlab.bsky.social @sarabiz86.bsky.social @andreabrandlab.bsky.social among other! @hjerling-leffler.bsky.social @ulrikakmarklund.bsky.social @flallemend.bsky.social
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Dr. Mar @boldnewme.bsky.social · 10/06/2026
Happy Wednesday.
In case you forgot:
We live on a planet where whales sing songs that travel for miles.
Where trees can recognize their own offspring and protect them underground.
Where dolphins give each other names and where lightning can create glass in the sand.
Where horses can read human emotions.
Where rain has a smell before it even arrives and where the ocean can glow in the dark.
And where stars we see might not even exist anymore.
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MwahahahahahadScientist @mads100tist.bsky.social · 10/06/2026
Look, people. We have superb keynotes, we are giving prizes, we are giving postdocs a megaphone to tell everyone why they should be hired. This is the meeting to be at. Sign up, join us, and have the time of your life while discussing science with people that know where the cleithrum is🐟🧪
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Katja Petzold @katjapetzold.bsky.social · 11/05/2026
The A-minor switch - check out our latest preprint (link below)
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Katja Petzold @katjapetzold.bsky.social · 08/05/2026
curious about dynamic states in DNA or better methods to detect them? Have a look at our recent work using 1H R1ρ RD NMR on hoogsteen to WCF equilibrium.
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Emma R Andersson @anderssonlab.bsky.social · 08/05/2026
Thank you! ❤️
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Emma R Andersson @anderssonlab.bsky.social · 08/05/2026
Incredibly proud and happy for @sandehaan.bsky.social , my former PhD student, who has won the Award for Best PhD thesis in Swedish Developmental Biology 2023-2025, from @swedbo.bsky.social (the Swedish Developmental Biology Organization)! Congratulations!! www.swedbo.se/2026/05/08/w...
swedbo.se
Winner of the SWEDBO best thesis award: Sandra de Haan – Swedish Developmental Biology Organisation
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Emma R Andersson @anderssonlab.bsky.social · 14/04/2026
Congratulations again - it was a pleasure examining your thesis! Great work, well-presented results and a fun discussion. Excited to see what you do next in your career 👏👏👏
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Katherine Brown @katherine-brown.bsky.social · 01/04/2026
"Why is publishing so expensive?" It's a question I hear all the time, so we at @biologists.bsky.social thought we'd try to dispel some of the myths around publishing economics & provide transparency around our costs. Today, we publish editorials in all 5 of our journals - links in the thread below.
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Gail Drakes @gaildrakes.bsky.social · 27/03/2026
Fun fact: A “Lord of the Flies” type thing actually DID happen…to a bunch of young boys of color (AHEM!) from Tonga… …and they did NOT devolve into violence and mayhem. They created their own rules and took care of each other until they were rescued by a fisherman over a year later!
saturdayeveningpost.com
The Real Lord of the Flies | The Saturday Evening Post
In this uplifting but nearly forgotten story, six boys were shipwrecked on a desert island for over a year. Instead of succumbing to their darker natures, they survived through friendship and cooperat...
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Emma R Andersson @anderssonlab.bsky.social · 20/03/2026
Interesting assumption that this metric would read out authorship inflation rather than interdisciplinary collaborative authors. Thanks for sharing - we’re in the middle of an institutional evaluation determining how to distribute resources based on “performance”, so this is interesting 🙃
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Emma R Andersson @anderssonlab.bsky.social · 11/03/2026
Fantastic work, @tovefall.bsky.social !
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Emma R Andersson @anderssonlab.bsky.social · 10/12/2025
Great work, Ole, congratulations! 🥳👏🤓
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MwahahahahahadScientist @mads100tist.bsky.social · 02/12/2025
Apply! It's a great experience and you get to meet very cool people. Let me know if you want to know more 🧪
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Enric Llorens @ellorens.bsky.social · 02/12/2025
First paper from the lab is now online @natneuro.nature.com ! We mapped injury induced enhancers in the mouse CNS and decoded their sequence architecture. Little 🧵 rdcu.be/eSQi1
rdcu.be
The regulatory code of injury-responsive enhancers enables precision cell-state targeting in the CNS
Nature Neuroscience - Zamboni et al. reveal how enhancers encode cell-type-specific responses to CNS injury. By combining multiomic profiling, deep learning and in vivo screening, they uncover...
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Emma R Andersson @anderssonlab.bsky.social · 15/11/2025
😂 Ditto! Despite my track record in agriculture… (Dear reader: she does not have a track record in agriculture…)
Screenshot of an email that reads “Dear Emma R. Andersson,
Cureus Journals, a part of Springer Nature, will be launching its fourth open access journal—Cureus Journal of Agriculture and Food Science (CJAF)— in December 2025. 
 
I would like to take this opportunity to invite you to apply for a position on our esteemed Editorial Board as an Associate Editor (AE) for CJAF.

Your expertise in agriculture and food science is highly regarded, and your research contributions have significantly advanced the field. “
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Michael Peel @mikepeeljourno.bsky.social · 05/11/2025
NEW: Children faced more risk of rare heart problems after Covid-19 infection than they did after taking the Pfizer/BioNTech mRNA vaccine, a big three-year study of under-18s in England has found. Important evidence for debates over vaccine safety, particularly in the US. www.ft.com/content/231d...
ft.com
Covid jab less likely than virus to cause rare heart issue in children
Largest study of its kind shows health risks of refusing vaccination
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Emma R Andersson @anderssonlab.bsky.social · 25/10/2025
Or: selective pressure and adaptation?
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Amanda Andersson-Rolf @amandaanderssonr.bsky.social · 21/10/2025
📣 I am excited to announce that I have joined the MBB department at Karolinska Institutet in Stockholm as an assistant professor. See below for more details! #wearehiring
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Alex Crits-Christoph @acritschristoph.bsky.social · 27/09/2025
Try following this feed, which are paper links shared by all the people you follow bsky.app/profile/did:...
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