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Alice Pyne

@alicepyne.bsky.social
2.4K followers 1.1K following 37 posts

UKRIFLF & professor of biophysics at the University of Sheffield. High-resolution #AFM and #biophysics studies of #DNA structure and function www.pyne-lab.uk 🔬🧬 she/her

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Reposted by Alice Pyne
Eddie Rollins @eddierollins.bsky.social · 30/03/2026
Some of my PhD work is now out in Nature! www.nature.com/articles/s41... Investigating the structural basis of off‑target Cas9 binding to supercoiled DNA with #AFM & cryo-EM. From my time in the lab of @alicepyne.bsky.social, in collaboration with @qmsmith.bsky.social and the team at the MRC LMS.
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Alice Pyne @alicepyne.bsky.social · 25/03/2026
Supercoiling not only brings DNA to life (and makes it dance) but also changes how key proteins such as #CRISPR interact with it. Thanks @qmsmith.bsky.social for the brilliant collaboration, Sylvia for developing an amazing new #imageanalysis pipeline & @eddierollins.bsky.social for beautiful #AFM.
CryoEM and AFM images of DNA minicircles bound to CRISPR Cas9
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Reposted by Alice Pyne
Quentin Smith @qmsmith.bsky.social · 25/03/2026
1/11 In our story out today in @nature.com , we showcase the molecular mechanisms that enable Cas9 to bind and cleave negatively supercoiled ((-)SC) off-targets! 🧬🧵 🔗 www.nature.com/articles/s41... #CRISPR #Cas9 #DNATopology #AFM #CryoEM #SingleMolecule #Biophysics
nature.com
Structural basis of supercoiling-induced CRISPR–Cas9 off-target activity - Nature
Cas9 structures explain topology sensing and off-target activation.
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Reposted by Alice Pyne
MRC Laboratory of Medical Sciences @mrc-lms.bsky.social · 25/03/2026
New research in Nature shows that the physical twisting of DNA plays an important role in DNA editing mistakes made by CRISPR. Using nanometer-sized circles of DNA, scientists captured never-before-seen interactions between CRISPR & DNA, providing insights that could eradicate errors altogether.
lms.mrc.ac.uk
DNA shape explains crucial gene-therapy challenges
CRISPR is a powerful DNA-editing tool that has underpinned huge advancements in human healthcare in the last decade. It is a precision tool, but is not perfect, and misplaced DNA edits can compromise ...
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Reposted by Alice Pyne
LUMICKS @lumicks.bsky.social · 14/01/2026
Organized with the @sheffielduni.bsky.social team: Matt Newton, @alicepyne.bsky.social & Ruby Peters. 👏 Speakers include Gijs Wuite, @jwelburn.bsky.social, @slfort.bsky.social, @akellett.bsky.social, @michellepeckham.bsky.social & Aakash Basu. Program & registration --> bit.ly/3NtxZlM
2nd Flyer includes names & titles of the SMBio26 Sheffield selected speakers
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Reposted by Alice Pyne
SPM4.0 @spm40projecteu.bsky.social · 20/05/2025
📐On #WorldMetrologyDay, we celebrate 150 years of measurement science! At #SPM40, precision at the nanoscale is our mission, advancing biomedical research through AI-powered microscopy. #MetrologyMatters #MSCA #ResearchEU
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Reposted by Alice Pyne
Kay Polland @kaypolland.bsky.social · 06/03/2025
Abstract deadlines coming up soon! Talk - 14th March Poster - 28th March Sign up on our Eventbrite: www.eventbrite.co.uk/e/glasgow-im... and come along and see our wonderful keynote speaker @alicepyne.bsky.social ! For more info, head to the GIN website : glasgowimagingnetwork.wordpress.com
eventbrite.co.uk
Glasgow Imaging Network Symposium 2025
Bringing together those from academia and industry, GIN provides a platform for delegates to share, promote and discuss microscopy.
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Reposted by Alice Pyne
Helen Matthews @helenkmatthews.bsky.social · 24/02/2025
🚨 PhD position available for Oct 25 🔬Using advanced microscopy to understand how keratin networks control tissue mechanics An interdisciplinary #cellbiology #biophysics collaboration with Ruby Peters & @alicepyne.bsky.social 👇 Apply by March 14 www.findaphd.com/phds/project...
Micrograph of keratin networks (green) and DNA (blue) within cells
Image by Rachel Lawson
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Alice Pyne @alicepyne.bsky.social · 17/01/2025
It’s been a busy start to the year with marking, paper and grant reviews and travel to SMB2025 in the US, I think we were delayed for as long as we were actually at the conference… Its good to be home in the Peak District with my best girl Tasso 🌅 🐕 ❤️
The Peak District from Stansted edge at sunset with a whippet looking out
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Reposted by Alice Pyne
RCSB Protein Data Bank @rcsbpdb.bsky.social · 06/01/2025
After a remarkable 300 columns, David Goodsell has retired from the Molecule of the Month. We are very grateful for his service. @jiwasa.bsky.social will continue the series, beginning with January's article on Assembly Line Polyketide Synthases Details: cdn.rcsb.org/rcsb-pd...
cdn.rcsb.org
EDUCATION CORNER: Molecule of the Month: Celebrating  25 Years of Storytelling and Announcing New Beginnings
After a remarkable 300 columns, David Goodsell has retired from the Molecule of the Month series.  Janet Iwasa will be continuing the series for PDB-101.
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Reposted by Alice Pyne
Andrew Kellett @akellett.bsky.social · 07/01/2025
Delighted to share our @NAR_Open paper on a click chemistry-derived DNA damaging agent 🧬✂️👏 Congrats to @westerlundlab.bsky.social @alicepyne.bsky.social @researchireland.bsky.social @dcuresearch.bsky.social @sspc.bsky.social @dublincityuni.bsky.social doi.org/10.1093/nar/...
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Reposted by Alice Pyne
Nynke Dekker Lab @nynkedekkerlab.bsky.social · 02/01/2025
Two more weeks to apply to our opening for a senior postdoc in biophysics; work on exciting projects in DNA replication and/or chromatin organization from an interest in instrumentation! Deadline Jan 15 and application link: my.corehr.com/pls/uoxrecru...
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Alice Pyne @alicepyne.bsky.social · 30/12/2024
I know - there’s a huge tree uprooted just after the stepping stones too - hope you’re having a lovely winter in the Southern Hemisphere!
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Alice Pyne @alicepyne.bsky.social · 30/12/2024
Today a Tasso jog through Endcliffe park - lots of storm damage with trees uprooted 🐕
A path with failed broken trees by the side
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Alice Pyne @alicepyne.bsky.social · 29/12/2024
Frosty twixmas adventures with Tasso 🤍💚
A man and a dog in a frost field with the sun just above a hill
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Alice Pyne @alicepyne.bsky.social · 28/12/2024
A few days of beautiful cloud inversions in the peak. Today a little exploration by bike 🚲- trying to stay above the cloud
A cloud inversion in the valley of the Peak District A cloud inversion behind some fields in the Peak District
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Alice Pyne @alicepyne.bsky.social · 27/12/2024
Boxing Day walks with Tasso, enjoying the #PeakDistrict and thinking how much my mum would have loved to have been with us 💚
Whippet Tasso in a green field under a bright blue sky
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Alice Pyne @alicepyne.bsky.social · 25/12/2024
First Christmas without maman, thinking of her lots and spending time enjoying the Peak District. She loved it up here 💚
View of the Peak District with the sun breaking through the clouds
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Alice Pyne @alicepyne.bsky.social · 23/12/2024
Celebrating the start of longer days with a beautiful sunrise on a morning trot with Tasso 🐕
Sunrise in the woods through the trees
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Alice Pyne @alicepyne.bsky.social · 16/12/2024
Only two days left to apply for this amazing opportunity to join Siân’s lab at Kings. Interested in data analysis, microscopy and working with an awesome PI? One not to miss 🔬 💻
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Reposted by Alice Pyne
Dan Bose @danbose.bsky.social · 05/12/2024
We have a #PhD available! I'm really excited about this project, looking at whether and how differences in the #IDRs of paralogous epigenetic enzymes CBP and p300 cause them to behave differently. www.findaphd.com/phds/project...
findaphd.com
Unveiling the Secrets of Intrinsically Disordered Proteins in Gene Regulation at University of Sheffield on FindAPhD.com
PhD Project - Unveiling the Secrets of Intrinsically Disordered Proteins in Gene Regulation at University of Sheffield, listed on FindAPhD.com
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FMI science @fmiscience.bsky.social · 16/12/2024
📣 Please share: We’re hiring a tenure-track Group Leader in Multicellular Systems to explore the molecular and cellular mechanisms driving the formation, maintenance, or destabilization of tissues, organs and organisms. Apply at: www.fmi.ch/education-ca...
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Reposted by Alice Pyne
Helen Matthews @helenkmatthews.bsky.social · 16/12/2024
Looking for a #biophysics PhD? There's still time to apply for our Yorkshire Biosciences project on how keratin networks affect the mechanical properties of cells & tissues. Interdisciplinary collab with Ruby Peters & @alicepyne.bsky.social. Deadline 6th Jan www.findaphd.com/phds/project...
findaphd.com
The role of keratin networks in maintaining epithelial tissue integrity at University of Sheffield on FindAPhD.com
PhD Project - The role of keratin networks in maintaining epithelial tissue integrity at University of Sheffield, listed on FindAPhD.com
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Alice Pyne @alicepyne.bsky.social · 16/12/2024
On my way to London for the @royalmicrosoc.bsky.social Data Analysis in Microscopy section meeting. Excited to catch up in person with everyone and chat all things 🔬 💻
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Alice Pyne @alicepyne.bsky.social · 15/12/2024
Gosh it’s beautiful isn’t it! See you there!
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Alice Pyne @alicepyne.bsky.social · 08/12/2024
A weekend of catching up with old pals from UCL in Hamburg. Tackling the grey drizzle with epic pastries 🥐
A butterfly shaped croissant
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Alice Pyne @alicepyne.bsky.social · 05/12/2024
We were so happy to have @raveentank.bsky.social join us for our first Insigneo Imaging Life workshop in Sheffield this week. It was an amazing day of microscopy🔬 and science 🧪 - thank you Raveen for braving the Manchester-Sheffield train line!
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Reposted by Alice Pyne
Helen Matthews @helenkmatthews.bsky.social · 26/11/2024
1/ Cancer cell biology MRC DiMeN project in collaboration with @maiblab.bsky.social & Liz Smythe to use recombinant biosensors to image active KRAS in #pancreaticcancer Apply by 13th Dec www.findaphd.com/phds/project...
findaphd.com
MRC DiMeN Doctoral Training Partnership: Understanding the membrane recruitment of oncogenic KRAS in pancreatic cancer at University of Sheffield on FindAPhD.com
PhD Project - MRC DiMeN Doctoral Training Partnership: Understanding the membrane recruitment of oncogenic KRAS in pancreatic cancer at University of Sheffield, listed on FindAPhD.com
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Reposted by Alice Pyne
Eddie Rollins @eddierollins.bsky.social · 22/11/2024
In this paper #AFM is used to image the chain-mail structure of kinetoplast #DNA from parasites and automated analysis unravels the complex molecular topology. 🧪 🔬 🧬 @alicepyne.bsky.social doi.org/10.1039/D4CP...
doi.org
Multiscale topological analysis of kinetoplast DNA via high-resolution AFM
Kinetoplast DNA is a complex nanoscale network, naturally assembled from thousands of interconnected DNA circles within the mitochondrion of certain parasites. Despite the relevance of this molecule t...
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Reposted by Alice Pyne
Martin Jones @martinjones.bsky.social · 21/11/2024
The @royalmicrosoc.bsky.social #DataAnalysisInImaging special issue of Journal of Microscopy is out now! onlinelibrary.wiley.com/toc/13652818... #ImageAnalysis #BioImageAnalysis #RMSDAIM
onlinelibrary.wiley.com
Special Issue: Data Analysis in Imaging: Journal of Microscopy: Vol 296, No 3
The <em>Journal of Microscopy</em> is the oldest journal dedicated to the science of microscopy, publishing the latest advances in microscopy techniques and novel areas of application.
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Alice Pyne @alicepyne.bsky.social · 20/11/2024
Come join us in Harrogate for a really exciting programme at the interface of the physical and life sciences! Abstract submission is now open, Harrogate is beautiful and the meeting will be really reasonably priced thanks to all our amazing sponsors 🥳
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Reposted by Alice Pyne
Catherine Eichhorn @cdeichhorn.bsky.social · 18/11/2024
I haven't seen a nucleic acids starter pack yet, so I started one! Please reach out if I missed you! go.bsky.app/NxKgUY5
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Reposted by Alice Pyne
The Lewis Lab @peterlewislab.bsky.social · 19/11/2024
"Dark TFs": new manuscript mapped 166 uncharacterized human transcription factors, finding half bind genomic "dark matter," often closed chromatin rich in transposable elements. www.biorxiv.org/content/10.1...
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Reposted by Alice Pyne
Sam Lord @samjlord.bsky.social · 18/11/2024
We have tutorials on how to generate SuperPlots in Prism, R, Python, and Excel in the paper, but you can also easily make SuperPlots using @joachimgoedhart.bsky.social's SuperPlotsOfData: huygens.science.uva.nl/SuperPlotsOf...
huygens.science.uva.nl
SuperPlotsOfData - Plots Data and its ReplicatesAbout
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Alice Pyne @alicepyne.bsky.social · 18/11/2024
How much snow will we get! Currently quite wet but settling… spot the chilly Tasso ❄️
A snowy city garden with a snowy grey sky and a fawn whippet in the middle
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Alice Pyne @alicepyne.bsky.social · 17/11/2024
When I finally arrived back in Sheffield (UK train nonsense) - it was so lovely to step off and head to Sheffield Open Studios at Persistence Works. I picked up some beautiful ceramics, wandered round the artists studios, and enjoyed the curated exhibition. I do so love Sheffield.
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Alice Pyne @alicepyne.bsky.social · 17/11/2024
She is v chic when not chasing squirrels 😬
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Alice Pyne @alicepyne.bsky.social · 17/11/2024
TYSM
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Alice Pyne @alicepyne.bsky.social · 17/11/2024
Tasso thanks you for allowing us in
Amass the whippet in a red beautiful jumper from Occam, staring out at the Peak District
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Alice Pyne @alicepyne.bsky.social · 17/11/2024
This looks super helpful!
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Alice Pyne @alicepyne.bsky.social · 17/11/2024
🙏 could you add me please?
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Reposted by Alice Pyne
George Heath @georgerheath.bsky.social · 15/11/2024
AFM starter pack. Sorry if I missed anyone, relatively small numbers at the moment. Please share, nominate others/yourself (any career stage). I will also try to add incoming new users regularly. go.bsky.app/MSz2t33
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Alice Pyne @alicepyne.bsky.social · 17/11/2024
Yes please!
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Alice Pyne @alicepyne.bsky.social · 17/11/2024
🙏
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Alice Pyne @alicepyne.bsky.social · 17/11/2024
😒
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Alice Pyne @alicepyne.bsky.social · 17/11/2024
We approaching Nottingham, where apparently there is a chance of tea arriving 🤞
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Alice Pyne @alicepyne.bsky.social · 17/11/2024
Less special to top it off with a 3 1/2 tea-less train home tackling paper reviews. I’m much better at separating work and weekends usually, but train work is an efficient way of putting a block of time aside, but is much better if tea is available 😭
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Alice Pyne @alicepyne.bsky.social · 17/11/2024
Extra special to stay with Inspiralis CEO and general super woman Natassja Bush, staying up too late drinking mint tea, reminiscing over a decade of knowing each other - time flies 🥰
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Anne Carpenter @drannecarpenter.bsky.social · 17/11/2024
If you’re making a starter pack, take a quick look at its demographics. Worth a bit of consideration!
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Alice Pyne @alicepyne.bsky.social · 17/11/2024
On the train home from a fantastic evening at the John Innes celebrating my long time collaborator and friend Tony Maxwell. I shouldn’t have been surprised by just how many people made the journey to Norwich to celebrate his incredible career, but it was still amazing to see. 🧬 ⭕️
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