iiinova — where your digital self keeps evolving

Reposted by Eddie Rollins

Royal Microscopical Society @royalmicrosoc.bsky.social · 26/06/2026

Thanks again to everyone who attended AFM & SPM Meeting 2026 in Leeds this week 🙂 🔬 We hope you had a great time and enjoyed all the science, discussions and collaborations over three fantastic days. Special thanks also to our generous sponsors for making this possible 👏

Logos of sponsors for the AFM & SPM Meeting 2026 including Bruker, Horiba, Oxford Instruments, and others arranged in a grid.

Overhead view of a busy indoor exhibition with multiple booths and attendees walking along a polished wooden floor in a grand hall.

031

Eddie Rollins @eddierollins.bsky.social · 12/06/2026

Want to batch-process AFM images without trial-and-error tuning? The afMLevel Python package automates AFM levelling with deep learning 🔬🧪 github.com/mayatek1/afM... CNN based workflows remove tilt & artefacts while preserving structure. #AFM #Python #MachineLearning #OpenScience #ResearchSoftware

github.com

GitHub - mayatek1/afMLevel: Machine Learning AFM levelling functions

Machine Learning AFM levelling functions. Contribute to mayatek1/afMLevel development by creating an account on GitHub.

160

Eddie Rollins @eddierollins.bsky.social · 05/06/2026

playNano v0.4.0 is now out! 🧪 Updates to visualisation and export makes easier to create publication quality images and videos. 🎨 NEW colormaps 📹 NEW export formats 🔬 NEW ARIS file reader INSTALL: pip install playnano github.com/derollins/pl... #AFM #HighSpeedAFM #OpenSource #Python #Biophysics

131

Reposted by Eddie Rollins

Nature @nature.com · 30/03/2026

Nature research paper: Structural basis of supercoiling-induced CRISPR–Cas9 off-target activity go.nature.com/4t39bk8

go.nature.com

Structural basis of supercoiling-induced CRISPR–Cas9 off-target activity - Nature

Cas9 structures explain topology sensing and off-target activation.

1143

Eddie Rollins @eddierollins.bsky.social · 30/03/2026

Some of my PhD work is now out in Nature! www.nature.com/articles/s41... Investigating the structural basis of off‑target Cas9 binding to supercoiled DNA with #AFM & cryo-EM. From my time in the lab of @alicepyne.bsky.social, in collaboration with @qmsmith.bsky.social and the team at the MRC LMS.

0173

Reposted by Eddie Rollins

Quentin Smith @qmsmith.bsky.social · 25/03/2026

1/11 In our story out today in @nature.com , we showcase the molecular mechanisms that enable Cas9 to bind and cleave negatively supercoiled ((-)SC) off-targets! 🧬🧵 🔗 www.nature.com/articles/s41... #CRISPR #Cas9 #DNATopology #AFM #CryoEM #SingleMolecule #Biophysics

nature.com

Structural basis of supercoiling-induced CRISPR–Cas9 off-target activity - Nature

Cas9 structures explain topology sensing and off-target activation.

26020

Reposted by Eddie Rollins

Alice Pyne @alicepyne.bsky.social · 25/03/2026

Supercoiling not only brings DNA to life (and makes it dance) but also changes how key proteins such as #CRISPR interact with it. Thanks @qmsmith.bsky.social for the brilliant collaboration, Sylvia for developing an amazing new #imageanalysis pipeline & @eddierollins.bsky.social for beautiful #AFM.

CryoEM and AFM images of DNA minicircles bound to CRISPR Cas9

04516

Reposted by Eddie Rollins

White Rose University Consortium @whiteroseuc.bsky.social · 05/06/2025

We're back in York today for day 2 of @prosperpostdoc.bsky.social and the group are looking at jobs! They've been tasked with finding job vacancies, researching the key information and skills needed for these roles outside of academia. #postdocs

A group of people looking at paper on the floor.

031

Reposted by Eddie Rollins

George Heath @georgerheath.bsky.social · 29/05/2025

Squeeze more out of your AFM images with NanoLocz v1.3 💪 Download the updated version here: github.com/George-R-Hea...

142

Eddie Rollins @eddierollins.bsky.social · 14/05/2025

Disappeared from here for a couple of months (social media doesn't come to me naturally) and it seems the wave has dissipated... is anyone who isn't a social media manager still here?

120

Eddie Rollins @eddierollins.bsky.social · 14/05/2025

Had the pleasure of chairing our Molecular and Nanoscale Physics group internal seminar at @physicsleedsuni.bsky.social today. Always good to celebrate success within the group and hear about the breadth of research going on. Today's talks covered topics from flow cytometry to polymer chemistry!

000

Reposted by Eddie Rollins

@physicsoflifeuk @physicsoflifeuk.bsky.social · 13/05/2025

Join us TODAY, 2-3 pm BST for our next ECR-led PoL seminar on Zoom. You can still get the Zoom link by registering here: forms.gle/RLmWdvUT8zsz... See you there!

011

Eddie Rollins @eddierollins.bsky.social · 13/05/2025

Enjoyed a day out of the office last week with this dynamic group of Yorkshire Postdocs. It was good to take some time to reflect on our values and strengths together. Excited for the next @prosperpostdoc.bsky.social day in York next month! #PostdocLife #AcademicNetworking

040

Reposted by Eddie Rollins

Roman Barth @romanbarth.bsky.social · 16/12/2024

My first post! Hi science community 👋🧬 We have been trying to figure out the mechanistic mysteries about SMC motor proteins. They extrude our genome constantly into loops. We know that that's important. But how it works, is a matter of intense debate! Today, we report a piece of the puzzle:

1266

Reposted by Eddie Rollins

Erin E. Cutts @eecutts.bsky.social · 16/12/2024

Please share: Still plenty of time to apply for PhD projects in my lab using single-molecule methods to look at disordered regions and condensin complexes! Contact me at e.cutts@sheffield.ac.uk For details on how to apply: www.findaphd.com/phds/project...

findaphd.com

Developing Imaging Solutions to Study the Dynamics of Human Condensin I at University of Sheffield on FindAPhD.com

PhD Project - Developing Imaging Solutions to Study the Dynamics of Human Condensin I at University of Sheffield, listed on FindAPhD.com

11314

Eddie Rollins @eddierollins.bsky.social · 16/12/2024

Back in the office after a long weekend in beautiful Bilbao. Had a great time exploring the city, eating Pintxos in the bars and visiting some amazing museums.

Puppy by Jeff Kooms, outside the Guggenheim museum in Bilbao.

030

Reposted by Eddie Rollins

Dan Bose @danbose.bsky.social · 05/12/2024

We have a #PhD available! I'm really excited about this project, looking at whether and how differences in the #IDRs of paralogous epigenetic enzymes CBP and p300 cause them to behave differently. www.findaphd.com/phds/project...

findaphd.com

Unveiling the Secrets of Intrinsically Disordered Proteins in Gene Regulation at University of Sheffield on FindAPhD.com

PhD Project - Unveiling the Secrets of Intrinsically Disordered Proteins in Gene Regulation at University of Sheffield, listed on FindAPhD.com

12126

Reposted by Eddie Rollins

Kumar Lab, UC Berkeley @kumarlabucb.bsky.social · 02/12/2024

Excited to share our new paper in @PNASNews on mixed, reconstituted neurofilaments (NFs)! This work was led by ChemE PhD student Erika Ding and done in collab w Prof. Rui Wang and ChemE PhD student Takashi Yokokura here at Berkeley pnas.org/doi/10.1073/...

183

Reposted by Eddie Rollins

Olivier Duss @olivierduss.bsky.social · 04/12/2024

First paper of our lab today @natureportfolio.bsky.social by Nussi! We provide a real-time movie on how #transcription and #translation cooperate using #single-molecule FM: we find long-range #ribosome/ RNAP communication mediated by #RNA looping! @embl.org #RNAbiology #RNASky t.co/a24Xxcxdo0

1525878

Eddie Rollins @eddierollins.bsky.social · 28/11/2024

This looks like a cool project with some cool people in a very cool city 🧪 🔬 #Bristol #AFM

070

Eddie Rollins @eddierollins.bsky.social · 28/11/2024

Interesting to see scientists perceived as a unifying force in society. 🧪 Not sure exactly what to think about this. It seems that several of the hot button issues at the moment have a scientific aspect so we (scientists) need to be aware how our voices are heard.

1246

Eddie Rollins @eddierollins.bsky.social · 28/11/2024

Super cool DNA nanotech- DNA builidng blocks that can be configured in 3D. 🧪 My inner 6 year old loves the dinosaur!

1141

Eddie Rollins @eddierollins.bsky.social · 26/11/2024

Dropping this on 🧪 Atomic force microscopy, DNA, and parasites! Examining the complex molecular structure of DNA chain mail #KDNA

010

Reposted by Eddie Rollins

George Heath @georgerheath.bsky.social · 15/11/2024

AFM starter pack. Sorry if I missed anyone, relatively small numbers at the moment. Please share, nominate others/yourself (any career stage). I will also try to add incoming new users regularly. go.bsky.app/MSz2t33

4228

Eddie Rollins @eddierollins.bsky.social · 14/11/2024

Essential reference for anyone presenting image data or derived analysis 🔬📹🔭🧪 A checklist for best practice when publishing light microscopy images but applicable to many other imaging fields including #AFM

231

Eddie Rollins @eddierollins.bsky.social · 13/11/2024

I remember when I was first told about DNA origami as an undergrad, it was introduced as a bit of a novelty that let you make smiley faces. I've since come to see how powerful it can be as a nanotechnology and a platform for nano/bio experiments.

111

Eddie Rollins @eddierollins.bsky.social · 13/11/2024

How long until I get comments about how I make my coffee? Hoping to tick off, "Shouldn't that be done in a lab" in the first week.

130

Reposted by Eddie Rollins

George Heath @georgerheath.bsky.social · 12/11/2024

Delighted to welcome @eddierollins.bsky.social to the group as a new postdoc! Eddie brings a wealth of expertise in bioAFM and will be applying his skills to develop high-speed AFM methods to better understand protein dynamics and interactions.

131

Eddie Rollins @eddierollins.bsky.social · 12/11/2024

A new account and a new start! Excited to be begining a postdoc role in Leeds and the School of Physics and Astronomy

The Sir William Henry Bragg Building, home of the School of Physics and Astronomy at the University of Leeds. It is autumn and the ground is littered with fallen leaves.

190