Reposted by Leon-Samuel Icking
I’m delighted to share our new pre-print:
Make it so: Rapid and affordable plasmid sequencing on ONT platforms with PICARD-seq
www.biorxiv.org/content/10.6...
I wanted to use a MinION to sequence my large repetitive plasmids in-house. Luckily @jwdebler.bsky.social was willing to help.
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biorxiv.org
Make it so: Rapid and affordable plasmid sequencing on ONT platforms with PICARD-seq
Plasmid construction underpins molecular biology and synthetic biology, yet validation is often limited to the inserted fragment rather than the whole plasmid, and around a third of laboratory-made pl...