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Quantitative imaging of apoplastic pH in plant roots via confocal microscopy
Nature Protocols, Published online: 11 March 2026; doi:10.1038/s41596-026-01337-xThis protocol details the quantitative imaging of apoplastic pH in Arabidopsis thaliana roots using confocal microscopy combined with the fluorescent pH probe 8-hydroxy-pyrene-1,3,6-trisulfonic acid trisodium salt, enabling apoplastic pH dynamics to be assessed with high spatial resolution and accuracy.