iiinova — where your digital self keeps evolving

Reposted by Jose Maldonado, PhD

LifeCanvas Technologies @lifecanvastech.bsky.social · 06/03/2026

Studying neural circuits is all about connectivity. So why cut up your sample? This #FluorescenceFriday, we're highlighting some mesmerizing 3D neural circuit data scientists have generated with our sample processing techniques and #SmartSPIM imaging on whole mouse brains.

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Reposted by Jose Maldonado, PhD

Keith Duncan @cygnusplantxray.bsky.social · 06/01/2026

Gratuitous X-ray microscopy imaging, a "bouquet" of developing soybean flowers. @zeiss-microscopy.bsky.social @danforthcenter.bsky.social

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Reposted by Jose Maldonado, PhD

New England Society for Microscopy @nesmicroscopy.bsky.social · 28/04/2025

"Rising from Chaos" #MicroscopyMonday: an active system of microtubules, kinesin motors and actin filaments. 🔬: Resonant Scanning Nikon AX-R Confocal 🥼: Quang Tran of the Fraden Lab, MRSEC, @brandeisuniversity.bsky.social 🏛️: www.brandeis.edu/science/reso... 📩: info@nesmicroscopy.org

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Jose Maldonado, PhD @josemald.bsky.social · 11/04/2025

Choroid plexus is often lost during tissue sectioning and overall anatomy can be difficult if not impossible to reconstruct. Here we observe the vascular anatomy of human choroid plexus as if through a jeweler’s loupe. Sample courtesy of Matthew Schrag and Neil Dani. #FluorescenceFriday

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Jose Maldonado, PhD @josemald.bsky.social · 20/03/2025

Thank you to the team at @syGlass for highlighting some of our data. If you haven’t tried working with your data in VR space, you really need to see what you are missing. Syglass is a powerful tool for visualizing and analysis. @vubasicsciences.bsky.social #microscopy #lightsheet #science 🧪

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Reposted by Jose Maldonado, PhD

Jennifer Groh @jmgrohneuro.bsky.social · 06/03/2025

I'm pleased to report that the board has now heard from NIH that the appointments of these three investigators and similarly situated folks are being extended. Thank you NIH! Delighted that the stellar work there will be continuing!

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Jose Maldonado, PhD @josemald.bsky.social · 05/02/2025

Here is a closeup on our survey of the #hippocampus from Joe Luchsinger’s mouse sample, using the 15x objective from LifeCanvas Technologies Inc. With #lightsheet imaging you can peel away layers and observe cellular anatomy with stunning detail. How can VNL help you image your sample?

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Reposted by Jose Maldonado, PhD

Andrew Octavian Sasmita @aosasmita.bsky.social · 31/01/2025

For #FluorescenceFriday, a side-by-side comparison of 5xFAD hemibrains at 6 months showing how Aβ plaques can be modulated not just by sex but also by the transgene parentage. More details from our recent paper where we reported the transgene inheritance effect in 5xFAD mice 👇

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Jose Maldonado, PhD @josemald.bsky.social · 28/01/2025

Just a gorgeous #Purkinje neuron all by itself in the cerebellum. Only with #lightsheet #microscopy on a whole #cleared sample could you ever hope to catch a lone reporter expressing cell in it’s entirety. #science 🧪

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Jose Maldonado, PhD @josemald.bsky.social · 20/01/2025

Definitely need to try this. Has anyone here used this on lightsheet meso scale data? @microscopy 🧪

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Jose Maldonado, PhD @josemald.bsky.social · 17/01/2025

Ok here’s a first go at looking at the previously acquired sample in 3D space. Still some work to do before we have a final product. 🧪

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Jose Maldonado, PhD @josemald.bsky.social · 16/01/2025

Image preprocessing. This is from the acquisition in my previous post. Final image will be gorgeous!

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Jose Maldonado, PhD @josemald.bsky.social · 13/01/2025

Thai is going to be a good one! Transgenic expression of tdTomato always yields phenomenal signal to noise.

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Jose Maldonado, PhD @josemald.bsky.social · 07/12/2024

Video games grow with their audience.

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Jose Maldonado, PhD @josemald.bsky.social · 06/12/2024

And the Rocky Mountains just shrug it off.

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Jose Maldonado, PhD @josemald.bsky.social · 05/12/2024

All the paint is still fresh for sharks.

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Jose Maldonado, PhD @josemald.bsky.social · 04/12/2024

Lightsheet microscopy is unparalleled in its ability to resolve whole circuits in the mouse brain. Michelle Bedebaugh let me image one of her Rabies MC3R Cre samples with the 15X objective on my smartSPIM and we are very happy with the results. 🧪

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Reposted by Jose Maldonado, PhD

Mike Ando @dmikeando.bsky.social · 22/11/2024

There has been enough interest that we are on to Microscopy Starter Pack #2. Still open to who to include, including yourself! go.bsky.app/DA4LAjJ

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Reposted by Jose Maldonado, PhD

Ali Maximilian Erturk @erturklab.bsky.social · 29/11/2024

Our new study shows that SARS-CoV-2 spike protein accumulates & persists in the body for years after infection, especially in the skull-meninges-brain axis, potentially driving long COVID. mRNA vaccines help but cannot stop it🔬🧠🦠🧵Your weekend read👇 @cellpress.bsky.social cell.com/cell-host-mi...

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Jose Maldonado, PhD @josemald.bsky.social · 29/11/2024

Big Brother says you have nothing to worry about.

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Reposted by Jose Maldonado, PhD

Søren Grubb @sorengrubb.bsky.social · 26/11/2024

Here we show how #microglia contact #pericytes and #endothelialcells in the #venule end of the #capillary bed. Note also how a #primarycilium of an #interneuron invaginates the microglia. But why? 🤔 journals.sagepub.com/doi/full/10.... @gazz4science.bsky.social

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Reposted by Jose Maldonado, PhD

Davi Bock @dddavi.bsky.social · 02/10/2024

Hard to overstate how pleased I am that the FlyWire consortium converted our FAFB ("Full Adult Fly Brain") EM volume into a connectome, and that the resulting science has been (and will be) so impactful. Thanks to everyone involved. #neuroscience www.nature.com/immersive/d4...

nature.com

The FlyWire connectome: neuronal wiring diagram of a complete fly brain

Artificial intelligence and human expertise meet to generate a map of all the connections in the fly brain. The resource is already being used by experimentalists and theoreticians to further our unde...

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Jose Maldonado, PhD @josemald.bsky.social · 22/11/2024

🧪 Tyrosine Hydroxylase is often the first probe labs try in order to validate their #clearing and labeling for #lightsheet #microscopy. It is robust, easy to identify and frankly, pretty. Need assistance with lightsheet? We are here to help at #Vanderbilt University. VanderbiltNL.com

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Jose Maldonado, PhD @josemald.bsky.social · 20/11/2024

Let’s see how well bsky does with external media. 🧪 #Vanderbilt Neurovisualization Lab is among the first to clear, label and image an intact NHP hemisphere. Please set your player to 4K! #lightsheet #microscopy #science #brain. If your lab wants to add lightsheet to your work we can help!

youtu.be

Marmoset brain hemisphere imaged with lightsheet

YouTube video by Neurovisualization Lab

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Reposted by Jose Maldonado, PhD

Michael Morehead @michael-syglass.bsky.social · 13/11/2024

Happy to connect here on bsky -- great to be here for #science, #neuroscience, #imaging, and #VR. Please add me! I've written a short blog on the silent 3D imaging revolution happening now in science. Check it out and let me know what you think! www.syglass.io/blog/unlocki...

syglass.io

Unlocking the Hidden Beauty of Science with Virtual Reality and Direct Volume Rendering — syGlass

Lightsheet? microCT? Serial block face electron microscopy? Have you ever heard of these technologies? Very few members of the public know of the silent imaging revolution happening right now in the l...

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Jose Maldonado, PhD @josemald.bsky.social · 19/11/2024

#Vanderbilt Neurovisualization lab specializes in #lightsheet #Microscopy. 🧪 Here we see a wave of c-fos positive cells in blue and tyrosine hydroxylase in gold. If you need help with lightsheet let me know!

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Jose Maldonado, PhD @josemald.bsky.social · 19/11/2024

One more #science video for today. Check out this forest of #neurons in a cleared #mouse #brain imaged with #lightsheet #microscopy at #Vanderbilt

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Jose Maldonado, PhD @josemald.bsky.social · 19/11/2024

GFAP expressing #astrocytes in orange, IBA1 positive microglia in blue. Images from unsectioned rat hemisphere. #lightsheet #microscopy at #Vanderbilt

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Jose Maldonado, PhD @josemald.bsky.social · 19/11/2024

Let’s get some #microscopy on bluesky going. Blood vessels in mouse brain imaged with #lightsheet microscopy #Vanderbilt

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