iiinova — where your digital self keeps evolving

Reposted by Jose Maldonado, PhD

LifeCanvas Technologies @lifecanvastech.bsky.social · 06/03/2026

Studying neural circuits is all about connectivity. So why cut up your sample? This #FluorescenceFriday, we're highlighting some mesmerizing 3D neural circuit data scientists have generated with our sample processing techniques and #SmartSPIM imaging on whole mouse brains.

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Reposted by Jose Maldonado, PhD

Keith Duncan @cygnusplantxray.bsky.social · 06/01/2026

Gratuitous X-ray microscopy imaging, a "bouquet" of developing soybean flowers. @zeiss-microscopy.bsky.social @danforthcenter.bsky.social

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Reposted by Jose Maldonado, PhD

New England Society for Microscopy @nesmicroscopy.bsky.social · 28/04/2025

"Rising from Chaos" #MicroscopyMonday: an active system of microtubules, kinesin motors and actin filaments. 🔬: Resonant Scanning Nikon AX-R Confocal 🥼: Quang Tran of the Fraden Lab, MRSEC, @brandeisuniversity.bsky.social 🏛️: www.brandeis.edu/science/reso... 📩: info@nesmicroscopy.org

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Jose Maldonado, PhD @josemald.bsky.social · 15/04/2025

I love a good olfactory bulb image.

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Jose Maldonado, PhD @josemald.bsky.social · 11/04/2025

Choroid plexus is often lost during tissue sectioning and overall anatomy can be difficult if not impossible to reconstruct. Here we observe the vascular anatomy of human choroid plexus as if through a jeweler’s loupe. Sample courtesy of Matthew Schrag and Neil Dani. #FluorescenceFriday

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Jose Maldonado, PhD @josemald.bsky.social · 20/03/2025

Thank you to the team at @syGlass for highlighting some of our data. If you haven’t tried working with your data in VR space, you really need to see what you are missing. Syglass is a powerful tool for visualizing and analysis. @vubasicsciences.bsky.social #microscopy #lightsheet #science 🧪

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Reposted by Jose Maldonado, PhD

Jennifer Groh @jmgrohneuro.bsky.social · 06/03/2025

I'm pleased to report that the board has now heard from NIH that the appointments of these three investigators and similarly situated folks are being extended. Thank you NIH! Delighted that the stellar work there will be continuing!

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Jose Maldonado, PhD @josemald.bsky.social · 05/02/2025

Here is a closeup on our survey of the #hippocampus from Joe Luchsinger’s mouse sample, using the 15x objective from LifeCanvas Technologies Inc. With #lightsheet imaging you can peel away layers and observe cellular anatomy with stunning detail. How can VNL help you image your sample?

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Reposted by Jose Maldonado, PhD

Andrew Octavian Sasmita @aosasmita.bsky.social · 31/01/2025

For #FluorescenceFriday, a side-by-side comparison of 5xFAD hemibrains at 6 months showing how Aβ plaques can be modulated not just by sex but also by the transgene parentage. More details from our recent paper where we reported the transgene inheritance effect in 5xFAD mice 👇

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Jose Maldonado, PhD @josemald.bsky.social · 01/02/2025

It’s always great to get lucky with a sample😆

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Jose Maldonado, PhD @josemald.bsky.social · 28/01/2025

Just a gorgeous #Purkinje neuron all by itself in the cerebellum. Only with #lightsheet #microscopy on a whole #cleared sample could you ever hope to catch a lone reporter expressing cell in it’s entirety. #science 🧪

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Jose Maldonado, PhD @josemald.bsky.social · 20/01/2025

Definitely need to try this. Has anyone here used this on lightsheet meso scale data? @microscopy 🧪

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Jose Maldonado, PhD @josemald.bsky.social · 18/01/2025

Hi Julia! My lab uses the smartSPIM from Life Canvas Technologies (Boston, Massachusetts) for imaging. This was cleared using their SMARTBATCH+

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Jose Maldonado, PhD @josemald.bsky.social · 18/01/2025

Ugh. Bsky is applying aggressive video compression and lots of detail is being lost.

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Jose Maldonado, PhD @josemald.bsky.social · 17/01/2025

Ok here’s a first go at looking at the previously acquired sample in 3D space. Still some work to do before we have a final product. 🧪

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Jose Maldonado, PhD @josemald.bsky.social · 16/01/2025

You know what it takes to get images like these! 😅

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Jose Maldonado, PhD @josemald.bsky.social · 16/01/2025

Thank you! I’m still rebuilding the entire volume.

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Jose Maldonado, PhD @josemald.bsky.social · 16/01/2025

Thank you Sunny! Transgenic expression is always the most photogenic!

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Jose Maldonado, PhD @josemald.bsky.social · 16/01/2025

These are tdTomato expressing neurons in a cleared, intact mouse brain.

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Jose Maldonado, PhD @josemald.bsky.social · 16/01/2025

Image preprocessing. This is from the acquisition in my previous post. Final image will be gorgeous!

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Jose Maldonado, PhD @josemald.bsky.social · 13/01/2025

Green fluorescent protein is being illuminated by a sheet of laser light. The protein is found inside of a population of neurons which together constitute a putative brain circuit. The sample is an intact mouse brain which has been chemically modified to be optically transparent.

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Jose Maldonado, PhD @josemald.bsky.social · 13/01/2025

Errr I meant ‘This’ is going to be a good one. Thai food sounds good for lunch though.

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Jose Maldonado, PhD @josemald.bsky.social · 13/01/2025

Thai is going to be a good one! Transgenic expression of tdTomato always yields phenomenal signal to noise.

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Jose Maldonado, PhD @josemald.bsky.social · 07/12/2024

Video games grow with their audience.

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Jose Maldonado, PhD @josemald.bsky.social · 07/12/2024

Those games are way more compelling than I ever game them credit for. Which of them is this in?

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Jose Maldonado, PhD @josemald.bsky.social · 06/12/2024

And the Rocky Mountains just shrug it off.

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Jose Maldonado, PhD @josemald.bsky.social · 05/12/2024

All the paint is still fresh for sharks.

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Jose Maldonado, PhD @josemald.bsky.social · 04/12/2024

Someone let me use picric acid once in grad school and they really shouldn’t have. I disposed of it safely but JUST barely. Just. Barely.

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Jose Maldonado, PhD @josemald.bsky.social · 04/12/2024

Is that accurate? Where did that number come from? Agreed on it seeming low and a lot.

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Jose Maldonado, PhD @josemald.bsky.social · 04/12/2024

Lightsheet microscopy is unparalleled in its ability to resolve whole circuits in the mouse brain. Michelle Bedebaugh let me image one of her Rabies MC3R Cre samples with the 15X objective on my smartSPIM and we are very happy with the results. 🧪

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Reposted by Jose Maldonado, PhD

Mike Ando @dmikeando.bsky.social · 22/11/2024

There has been enough interest that we are on to Microscopy Starter Pack #2. Still open to who to include, including yourself! go.bsky.app/DA4LAjJ

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Reposted by Jose Maldonado, PhD

Ali Maximilian Erturk @erturklab.bsky.social · 29/11/2024

Our new study shows that SARS-CoV-2 spike protein accumulates & persists in the body for years after infection, especially in the skull-meninges-brain axis, potentially driving long COVID. mRNA vaccines help but cannot stop it🔬🧠🦠🧵Your weekend read👇 @cellpress.bsky.social cell.com/cell-host-mi...

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Jose Maldonado, PhD @josemald.bsky.social · 30/11/2024

He stated this specifically. His wife, some of his kids and, all of his friends had passed.

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