iiinova — where your digital self keeps evolving

Howard Salis @hsalis.bsky.social · 24/03/2026

Pfft with your “reproducibility”. If a plot needs to be updated, I start by re-ordering all the reagents and (my students) going back into the lab to collect all the data again. That’s reproducibility. /jk but it’s a fun way to argue that there is no strictly universal definition of reproducible.

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Howard Salis @hsalis.bsky.social · 27/07/2025

Passing through Coralville, IA! Shout out to IDT!!

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Howard Salis @hsalis.bsky.social · 21/07/2025

That tiniest of moments when a devops restarts their load balancer

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Howard Salis @hsalis.bsky.social · 10/07/2025

Need to remove any repetitive DNA via redesign …

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Howard Salis @hsalis.bsky.social · 22/06/2025

Putida is also used in bioconversion of cellulosic hydrolysate to FDCA (2,5-Furandicarboxylic acid)

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capitolhunters @capitolhunters.bsky.social · 15/06/2025

It's an OK article but the tag line should be "Trump illegally cancels Northwestern's contracts and refuses to pay them", not "Trump blew up Northwestern's business model". If you hold up a bank at gunpoint and abscond with bags of money you're not "blowing up its business model". You're stealing.

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Howard Salis @hsalis.bsky.social · 15/06/2025

All these ML-centric rewordings of old ideas is getting tired. Next someone will write a perspective comparing evolution and reinforcement learning. Or ensemble modeling as wisdom of the crowd. Boring!

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Howard Salis @hsalis.bsky.social · 13/06/2025

EPIGENETIC HULK WAS WAITING FOR GREENSKY. EPIGENETIC HULK THEN REALIZED THAT HIGH-ENERGY SMASH CAUSES BLUE-SHIFT. MORE SMASH MORE BLUESKY

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Howard Salis @hsalis.bsky.social · 21/03/2025

Every compiler error in tech would be a dead person in biotech.

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Howard Salis @hsalis.bsky.social · 21/03/2025

What a shitty time to run out.

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Jason Rasgon @vectorgen.bsky.social · 10/03/2025

As a Jew looking at the administration's sudden "concern" over University antisemitism

media.tenor.com

a woman in a plaid shirt says that 's bullshirt

ALT: a woman in a plaid shirt says that 's bullshirt

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Mark Peifer (He, him) @peiferlabunc.bsky.social · 01/03/2025

In a remarkable new paper, Qureshi and Duss use multi-colour single-molecule fluorescence microscopy to directly and simultaneously track transcription, translation & coupling 3/n www.nature.com/articles/s41...

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Mark Hamill @markhamillofficial.bsky.social · 28/02/2025

Could not agree more. The Prez & VP are shameful, embarrassing Putin puppets & a disgrace to our country. 🤮 #SlavaUkraine 🇺🇦

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A.R. Moxon @juliusgoat.bsky.social · 20/02/2025

Normalize calling Republicans Nazis.

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Howard Salis @hsalis.bsky.social · 18/02/2025

Wowzers the SNL 50th anniversary special was truly fantastic and man did I need to laugh a bit to cleanse the soul after the last two weeks. My favorite bit: youtu.be/l8GbTZ8v1-w?... The whole Shebang is streaming on Peacock.

youtu.be

New York 50th Musical – SNL50

YouTube video by Saturday Night Live

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Howard Salis @hsalis.bsky.social · 16/02/2025

Excellent reason to only use defined, designed barcodes (flanking inserts) and identify/filter out any discordant barcode pairs. Random barcodes == you never know what you're going to get!

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bioRxiv Synthetic Biology @biorxiv-synthbio.bsky.social · 16/02/2025

Simultaneous in vitro expression of minimal 21 transfer RNAs by tRNA array method www.biorxiv.org/content/10.1101/202…

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Jeremy Berg @jeremymberg.bsky.social · 14/02/2025

I am hearing that about 1/2 of the ARPA-H staff were probationary and are being let go. Many of these folks are long-time NIHers who were promoted into new positions in ARPA-H and became probationary in these new positions despite many years of NIH service.

media.tenor.com

a man is standing in front of a mirror talking to another man and says `` my god you cannot be that stupid '' .

ALT: a man is standing in front of a mirror talking to another man and says `` my god you cannot be that stupid '' .

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Angel Goñi-Moreno @angelgm.eurosky.social · 14/02/2025

Today's the day! Getting ready for the workshop on #AI in #Biology. While these fields aren’t new (of course!), the intersection of the two is having a major moment right now.

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Shockratees @thatshockratees.bsky.social · 11/02/2025

Ok folks. Time for me to pull my law rank and correct some shit. When a judge issues a civil contempt order, she does not hand it to the prosecutor or AG and say “enforce this if you feel like it.” The judge hands it directly to a law enforcement officer, who has no discretion on whether to serve it

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Victor de Lorenzo (CNB, Madrid) @vdlorenzo.bsky.social · 14/02/2025

Thrilled to have @hsalis.bsky.social as the keynote speaker for today’s workshop on AI in Biology! After a prologue of intense discussions, he follows the tradition of every visitor by stepping onto Km 0 at Puerta del Sol—the geographical center of Spain & a place of significance for every Madrileño

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Howard Salis @hsalis.bsky.social · 12/02/2025

I feel sorry for anyone studying phospho-diesterase I (pDEI). ecocyc.org/gene?orgid=E...

ecocyc.org

Escherichia coli K-12 substr. MG1655 pdeI

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Josh Gerstein @joshgerstein.bsky.social · 11/02/2025

BREAKING: Federal judge in Boston broadens block on Trump's NIH grant 15% overhead limit (indirect cost rates) to all recipients nationwide. Doc: www.documentcloud.org/documents/25... #TROpalooza ain't done

documentcloud.org

AAMCOrd021025

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Howard Salis @hsalis.bsky.social · 10/02/2025

Parties to suit: Massachusetts, Illinois, Michigan, Arizona, California, Connecticut, Colorado, Delaware, Hawaii, Maine, Maryland, Minnesota, Nevada, New Jersey, New Mexico, New York, North Carolina, Oregon, Rhode Island, Vermont, Washington & Wisconsin. WTF no Pennsylvania???

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Howard Salis @hsalis.bsky.social · 09/02/2025

What if CA, MA, & other high-GDP states passed State Law to redirect all federal tax collection to a State bureau, which would only redirect taxes to the Fed if Federal Law wasn’t blatantly ignored (by vote of State legislature)? Another lever of “checks & balances” & a “soft secession” when needed

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Katie Howland @katiehowland.bsky.social · 09/02/2025

Buckle up babes, it's time for a long thread about #USAID. Many of you know I've worked on USAID contracts and in humanitarian aid for the last decade. But very few know why. I studied international security in undergrad and my first job out of school was in the defense/intel world. I hated it. 1/

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Howard Salis @hsalis.bsky.social · 08/02/2025

Canada removed US liquor from all of its stores (something like 30% of US liquor exports). Even liquor isn’t a safe bet from Trump’s mayhem. :(

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Library Innovation Lab @harvardlil.bsky.social · 06/02/2025

We just launched a 16TB archive of every dataset that has been available on data.gov since November. This will be updated day by day as new datasets appear. It can be freely copied, and we're sharing the code behind it to help others make their own archives of data they depend on.

lil.law.harvard.edu

Announcing the Data.gov Archive | Library Innovation Lab

Today we released our archive of data.gov on Source Cooperative. The 16TB collection includes over 311,000 datasets harvested during 2024 and 2025, a complet...

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Howard Salis @hsalis.bsky.social · 05/02/2025

the crater from a 2000 lb bomb makes an excellent sand trap 🫤

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Howard Salis @hsalis.bsky.social · 05/02/2025

OMG the troll that demands that the U.S. stay out of world affairs now wants *us* to OWN the most controversial land in the world. NO NO NO NO NO NO NO NO NO NO. Sure looks like Trump is a Russian asset. Is it Stupidity or Treason? If you can't tell ...

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Andy Fraser @andyfraser.bsky.social · 04/02/2025

Delighted to share our latest paper describing a method to read the levels of hundreds of metabolites or drugs in parallel using DNA sequencing. This method, which we call ‘smol-seq’ (Small MOLecule sequencing), harnesses the power of DNA sequencing for metabolite detection: rdcu.be/d8xLv (1/6)

rdcu.be

Quantifying metabolites using structure-switching aptamers coupled to DNA sequencing

Nature Biotechnology - Metabolites can be quantified using a combination of aptamers and DNA barcodes.

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Lauren Bauer @laurenhlb.bsky.social · 03/02/2025

New and important: we built a federal expenditure tracker. All expenditure line items that are available on the Daily Treasury Statement. USAID was zeroed out on 1/28 and has been at zero ever since. www.hamiltonproject.org/data/trackin... #EconSky

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Justin Brower @naturespoisons.bsky.social · 03/02/2025

I won't be around for ToxTueday, so you'll have to settle for ToxMonday, instead. Today, I'm posting an experiment I did about 5 years ago with my old lab for some "team building." So what did we do? Look for drugs on money, of course! What did we find? Lots, but you'll have to keep reading:

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Howard Salis @hsalis.bsky.social · 03/02/2025

No, I did not say that (and I don't think that statement is true). Let's focus our energy on the real battle :)

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Howard Salis @hsalis.bsky.social · 03/02/2025

Bernie's party-wide email! (One of the few I actually read)

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Howard Salis @hsalis.bsky.social · 01/02/2025

Hmm, once some sysape starts shutting down web services & databases (like we see here), it can "accidentally" expose private data & infrastructure keys. The blackhats are going to have a field day poking around.

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Waggoner Lab @labwaggoner.bsky.social · 31/01/2025

Ribosomes cooperate through transient collisions to ensure efficient translation www.cell.com/cell/fulltex... @cellcellpress.bsky.social @marvintanenbaum.bsky.social

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Howard Salis @hsalis.bsky.social · 31/01/2025

Oh, I totally agree, but if we're going to apply Trump's definition of DEI (not mine!!) to science funding, then that is what happens. None of this is fair or reasonable or intelligent. I voted for Kamala.

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Nucleic Acids Research @narjournal.bsky.social · 31/01/2025

Hello Everyone! 👋 Nucleic Acids Research is excited to be on Bluesky! Please follow us for the latest #Research, #Breakthrough articles, journal issues and more. Also, please consider adding us to your starter packs!

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Howard Salis @hsalis.bsky.social · 31/01/2025

Sad to say, but Red States are often the DEIs of Science funding.

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Howard Salis @hsalis.bsky.social · 29/01/2025

1. Steal colonies 2. ???? 3. Profit !

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Howard Salis @hsalis.bsky.social · 29/01/2025

You are not required to comply with an unlawful order. So don’t!

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Howard Salis @hsalis.bsky.social · 29/01/2025

Does Trump have the legal right to pause or stop federal programs because he doesn’t like them? Short answer: no. Congress passed the Impoundment Control Act to prevent presidents from doing exactly this (especially Nixon). www.nytimes.com/2025/01/28/u...

nytimes.com

Does Trump Have the Power to Block Spending That Congress Has Authorized?

The White House’s temporary pause on trillions in federal spending could set up a court fight over executive authority and Congress’s control of the purse.

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