Howard Salis @hsalis.bsky.social · 03/02/2025Bernie's party-wide email! (One of the few I actually read) 191 9
Howard Salis @hsalis.bsky.social · 02/12/2024Finished?! "Create an image of an engineer in a hamster wheel running while juggling pipettes with a laptop on their head." 020 2
Howard Salis @hsalis.bsky.social · 02/12/2024We engineered sensor-circuits using our Promoter Calculator model to fine-tune sense and antisense transcription rates. The circuit converts transient TNT detection to a long-term response. We carried out flow cytometry to ensure a uniform response in liquid culture across low TNT concentrations. 240 4
Howard Salis @hsalis.bsky.social · 02/12/2024We started by engineering a new riboswitch sensor that directly binds to TNT and regulates translation rate. We applied our Riboswitch Calculator to rationally design two sequences, integrate them into B. subtilis genome, and test them in liquid media & soil systems. Both designs worked well. 150 5
Howard Salis @hsalis.bsky.social · 22/11/2024Why is my feed polluted with loud-bipeds-that-feed-me? ;) 100 1
Howard Salis @hsalis.bsky.social · 13/11/2024We then combined biophysical predictions (RBS Calculator & Promoter Calculator) & mRNA features to train a ML model that predicted steady-state mRNA levels & kinetic mRNA decay rates. The model has only 496 features, but achieves high accuracy & generalizability! 110 1
Howard Salis @hsalis.bsky.social · 13/11/2024We began by designing barcoded mRNAs with systematically varied sequence, structural, and functional motifs. In a massively parallel assay, we treated cell with rifampicin to turn off TX initiation, collected mRNA across timepoints, and ran deep sequencing. Kinetic measurements from NGS! 110 1
Howard Salis @hsalis.bsky.social · 13/11/2024If Hesgeth is even confirmed, he’s going to find running the largest bureaucracy in the world to be a bit outside his expertise …. 010 1
