This protocol can be used to extract High Molecular Weight gDNA from bacterial and archaeal cultures, resulting in gDNA suitable for long-read sequencing. It was optimized for Ignicoccus but also test...

In 1977, Woese and colleagues revealed Archaea as a distinct domain of life. Building on this insight, the discovery of Asgard archaea has strengthened the view that many hallmarks of eukaryotic cell…

Hibernation factors preserve ribosomes during cellular dormancy. Here, the authors identify a family of archaeal hibernation factors and use genetic, biochemical, and structural studies to show how th...

To prepare for DNA replication, a short region of DNA base-pairs must be broken, termed melting, allowing a helicase to gain a foothold on an isolated DNA strand. Here, authors use single-particle cry...

Abstract. Ammonia-oxidizing archaea (AOA) have repeatedly been detected with molecular methods on human skin, yet their persistence, physiological traits,

Microorganisms employ inorganic polyphosphate (polyP) as an ancient strategy for energy and phosphate storage, yet its physiological roles in metha...

The domain Archaea comprises diverse life forms distinguished by their unique evolutionary history, cellular structures, and metabolic pathways. Classified initially as “extremophiles,” archaea are now well known to inhabit a wide range of moderate...

The genomes of organisms across the tree of life are structurally and functionally organized into chromatin. In eukaryotes, within an organelle called the nucleus, chromatin is shaped by histones and ...

Abstract. Environmental genomics has led to the discovery of many new lineages of archaea, including “DPANN” (or Nanobdellati), comprising organisms with s

Microorganisms within the Methanoperedenaceae family of archaea can catalyze anaerobic methane oxidation. In this Review, Liu et al. reflect on the history and challenges of Methanoperedenaceae research, covering both the biology and the potential applications such as in wastewater treatment.

Acetate produced by methanogens from captured methane and carbon dioxide can be converted efficiently to ethanol, demonstrated by lab experiments on the engineered methanogen Methanosarcina acetivoran...

Abstract. Environmental genomics has led to the discovery of many new lineages of archaea, including “DPANN” (or Nanobdellati), comprising organisms with s

This protocol is an adapted form of the protocol developed for imaging haloarchaea (Liao et al., 2021) and subsequently applied to co-cultures containing nanohaloarchaea and haloarchaea (Hamm et al., ...

Archaeal research and its growing importance have benefited from a community that is engaged in various collaborative efforts, which are highlighted here with examples for the sharing of resources and...