iiinova — where your digital self keeps evolving

ZiyanWang @ziyannwang.bsky.social · 10/10/2026

We hope our in situ findings bring exciting new insights to the centriole & centrosome field! Would love to hear thoughts from the community: #Centriole #cryoET #InSituStructuralBiology @centriolelab.bsky.social @cellarchlab.com @computingcaitie.bsky.social @alanbrownhms.bsky.social

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ZiyanWang @ziyannwang.bsky.social · 09/10/2026

3/4 We further uncovered luminal γ-tubulin ring complexes (γ-TuRCs) anchored to the MT wall via the augmin complex — candidate nucleation sites for central pair (CP) biogenesis! we also surprisingly found tube-like densities underneath the luminal ring which may be the selective gate?

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ZiyanWang @ziyannwang.bsky.social · 09/10/2026

2/4 Resolving native BBs at subnanometer resolution in situ, we identified 11 ciliary-base microtubule-associated proteins (CMAPs, including 5 novel core components) specifically orchestrating the triplet-to-doublet transition along the proximal-distal axis. 🔬

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ZiyanWang @ziyannwang.bsky.social · 09/10/2026

🚨 Excited to share our latest work deciphering the native ciliary base! Using in situ #cryoET, we resolved the subnanometer architecture of native mammalian BBs in multiciliated cells! 🧵👇https://www.biorxiv.org/content/10.64898/2026.10.08.757802v1

biorxiv.org

In situ architecture of the basal body reveals regionalized assembly and central pair microtubules nucleation

The basal body (BB) is essential for axoneme assembly and ciliary function, and its defects are associated with ciliogenesis disorders and ciliopathies (1,2). However, the molecular basis of the BB's ...

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Haixia Zhou @haixia.bsky.social · 10/09/2026

First day here and I brought a paper. Three years of cryo-FIB-ET on human airway cells, nine proteins identified and two new PCD genes at the end of it. Grateful to the teams in Munster (Omran Lab), Boston (@alanbrownhms.bsky.social, Hawkins Lab) and Geneva (@centriolelab.bsky.social).

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Oli Clarke @olibclarke.bsky.social · 11/06/2026

The results here re 3D classification are quite impressive. Would be interesting to see how much the LPP improves classification of near-native samples with extreme compositional heterogeneity (e.g. derived from cellular fractions)

science.org

Laser phase plate improves structure determination of small proteins by cryo-EM

Phase plates can in principle overcome the poor image contrast in electron cryo–microscopy (cryo-EM) and the resulting limits on the structural reconstruction of small proteins. However, previous desi...

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cryoEM papers @cryoempapers.bsky.social · 30/05/2026

CAMSAP2 condensates drive a γ-TuRC-independent pathway for non-centrosomal microtubule nucleation www.biorxiv.org/content/10.64898/2026.05.28.728596v1 #cryoEM

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Dimitry Tegunov @dtegunov.bsky.social · 03/05/2026

We're super excited to share MissAlignment: a new ML-based approach to reference-free tilt series alignment, spearheaded by @martenchaillet.bsky.social. We think it's going to make your cryo-ET life a lot better. Preprint: www.biorxiv.org/content/10.6... Code: github.com/warpem/miss-... 🧶 Thread:

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Carter Lab @carter-lab.bsky.social · 27/04/2026

Always looking for ways to improve cryo-ET lamella quality. In last week’s #journalclub, we discussed what seems to be a promising and practical in-chamber sublimation approach for mitigating ice contamination and curtaining during lamella prep. Preprint-- www.biorxiv.org/content/10.6...

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Science News @scinews.bsky.social · 19/04/2026

Great!😂 @cellpress.bsky.social Garlic inhibits mating and egg laying in Aedes vector mosquitoes

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KaufmannLab @kaufmannlab.bsky.social · 24/04/2026

Happy to see the preprint of our VULCROM setup online! Thanks to our amazing collaborators we've managed to get some pretty cool cryo-CLEM images by combining cryo super-resolution and cryo-ET: doi.org/10.64898/202...

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Helena Watson @helenawatson.bsky.social · 25/04/2026

Preprint 🧵! I’m really excited to introduce BIGSMALL, an interleaved multi-magnification cryo-ET scheme bridging molecular and cellular scales in a single acquisition! BIGSMALL = 𝐁road 𝐈nformation 𝐆athering 𝐒trategy by 𝐌ultiscale 𝐀cquisition of lame𝐋𝐋a www.biorxiv.org/content/10.6... (1/6)

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Ben Engel @cellarchlab.com · 14/04/2026

Across scales from cells🦠 to atoms⚛️ – We reveal how anaerobic #bacteria break down very stable aromatic compounds found e.g. in oil spills 🛢️ #Bioremediation #TeamTomo 🧪 🧶🧬 🔬 Awesome collab with Lena, Matthias, @schullerjm.bsky.social @rnfr2d2.bsky.social @tomaspascoa.bsky.social @tamb-o.bsky.social

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Ben Engel @cellarchlab.com · 08/04/2026

It’s done!! Molecular architecture of the ciliary base in mammalian multiciliated cells! A study 13 years in the making 👴, thanks to collab with @stearnslab.bsky.social + @centriolelab.bsky.social & visionary work by @computingcaitie.bsky.social, who integrated native #cryoET with #XLMS & #UExM 🧪🧶🧬🔬

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Carter Lab @carter-lab.bsky.social · 24/03/2026

Excited to share our work on the structure and function of cytoplasmic lattices within mouse embryos. A collaborative effort with @niakanlab.bsky.social and work led by @kashishsingh.bsky.social and @inaharasimov.bsky.social . It is now out on BioRxiv: www.biorxiv.org/content/10.6...

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Bui Lab @McGill @builab.bsky.social · 23/12/2025

Tired of drawing cilia or centrioles for slides and papers? I feel you. Check out CiliaBuilder for ChimeraX → cxtoolshed.rbvi.ucsf.edu/apps/chimera... Build custom 3D cilia & centriole models (tips + primary cilia included).

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Raff Lab @jrafflab.bsky.social · 30/03/2025

🔬 Exciting findings from our collaborative study with Lea, Raff, van Breugel&Feng Lab! We've uncovered how Spd-2/CEP192 proteins with an SP2D structure are crucial for centrosome assembly. Crystal structures&mutations reveal new insights into PCM scaffold formation. www.science.org/doi/10.1126/...

science.org

The conserved Spd-2/CEP192 domain adopts a unique protein fold to promote centrosome scaffold assembly

A conserved structural domain within CEP192/Spd-2 protein promotes centrosome scaffold assembly during centrosome maturation.

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Paul & Virginie - Centriole Lab @centriolelab.bsky.social · 17/03/2025

Thrilled to have collaborated with the Lansky/Braun Lab on this exciting project: Cell-free Reconstitution Reveals Synergistic Stabilization of Microtubule Doublets by PACRG and FAP20 ! www.biorxiv.org/content/10.1...

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