This Review considers developments in enzymes, biosynthetic pathways and cellular engineering that enable their use in catalysis for new chemistry and beyond.

A series of spontaneously blinking dyes in the far-red range facilitate single-molecule localization microscopy. These dyes vary in their blinking properties and can be matched to the applications and...

Background Terpene synthases (TPSs) are enzymes that catalyze some of the most complex reactions in nature—the cyclizations of terpenes, which form the carbon backbones to the largest group of natural...

Tan and colleagues develop DNA nanodevices to detect the pH of the lysosomal outer surface, observing an acidic layer generated by TMEM175 that regulates lysosome positioning in response to changes in...

Abstract. Site-specifically attaching biorthogonal handles to proteins is an essential tool for chemical biology research and diverse applications, includi

Abstract. Antimicrobial resistance (AMR) is a global health threat, yet, despite this, antibiotic drug discovery has stagnated. Most compounds entering the

An iron-based, enzyme-triggered CO-releasing molecule (ET-CORM) is site-specifically conjugated to the antibody trastuzumab. This novel ET-CORM-antibody conjugate releases carbon monoxide upon cleava...

A chemoproteomic approach is developed that examines changes in ligand binding-induced accessibility by globally labeling reactive proteinaceous lysines, revealing the cellular targets of glycolytic i...

A photoswitchable glucocorticoid receptor agonist enables optical control of receptor activity, achieving picomolar potency and partial agonism that preferentially maintains anti-inflammatory signali...