Reposted by Sriram SVikram Chandra @vchandra.bsky.social · 09/09/2026I am excited to announce that I will start the Brain Origins Lab at the Crick in March 2027! Using acoels as a model system, the lab will study how animal brains evolve, and how innovations in brain organisation enable new computational abilities and the evolution of complex behaviour. 1111326
Reposted by Sriram SBob Johnston @bobbyjeyeguy.bsky.social · 25/08/2026Happy to share our review by Allie Reed from my lab together with Karina Chaudhari and Alex Kolodkin discussing the conservation of retinal direction-selective circuits across vertebrates! www.sciencedirect.com/science/arti...sciencedirect.comConservation of retinal direction-selective circuits across vertebrate speciesRetinal circuits extract visual features such as color, contrast, and motion. Direction-selective (DS) circuits provide a well-studied model for under… 083
Sriram S @srirams433.bsky.social · 18/07/2026#FluorescenceFriday Never let go: Oligodendrocyte (green) hold on to axon (magenta) 091
Reposted by Sriram SSamantha Butler @samanthabutler.bsky.social · 10/07/2026Looking for a great @cshlmeetings.bsky.social CSHL meeting this Fall? Abstract submission is open for "Molecular Mechanisms of Neuronal Connectivity" until July 22nd. Join me, @dacolon.bsky.social, Elva Diaz and @schuldinerorenlab.bsky.social at the bar! meetings.cshl.edu/meetings.asp...meetings.cshl.eduMolecular Mechanisms of Neuronal ConnectivityCold Spring Harbor Laboratory Meetings & Courses -- a private, non-profit institution with research programs in cancer, neuroscience, plant biology, genomics, bioinformatics. 076
Sriram S @srirams433.bsky.social · 10/06/2026And a handy table of neuron types, their molecular markers, projection targets, and the few known molecular determinants of these patterns: tinyurl.com/59549vnc Hope to see that last column fill up soon thanks to exciting new techniques coming up to perturb and observe neuronal projections in vivocell.com 000
Sriram S @srirams433.bsky.social · 10/06/2026And recently identified molecular determinants of some of these patterns: 100
Sriram S @srirams433.bsky.social · 10/06/2026Featuring distinct projection and branching patterns of different laminar and areal subtypes of cortical pyramidal neurons: 100
Sriram S @srirams433.bsky.social · 10/06/2026Great to see our review of axon branching in the cortex featured on the cover of this months issue of @cp-trendsneuro.bsky.social! 172
Sriram S @srirams433.bsky.social · 11/04/2026'Creation of Myelin': Oligodendrocyte (green) process reaches out and wraps around a neuronal axon (magenta) #FluorescenceFriday 041
Reposted by Sriram SRobert Hill @bobalhill.bsky.social · 06/03/2026Megan Doty et al discovered that myelin remains for weeks after cortical axons degenerate. We call the sheaths without an axon de-axoned myelin. During the slow, asynchronous, degeneration, microglia don't play a major role in clearing the axon or de-axoned myelin. www.science.org/doi/10.1126/... 1309
Reposted by Sriram SbioRxiv Neuroscience @biorxiv-neursci.bsky.social · 08/01/2026Protein tyrosine phosphatase receptor type kappa (PTPRκ) regulates Superior ON-Direction Selective Ganglion Cell development, facilitating image stabilization www.biorxiv.org/content/10.64898/20… 044
Reposted by Sriram SOneNeuro Initiative @oneneurojhu.org · 19/12/2025The winner of OneNeuro's November #FotoFriday #FluorescenceFriday contest is "Portrait of a Claustrocortical Neuron" submitted by Baldomero B. Ramírez Cantú in the Solange Brown Lab at @hopkinsmedicine.bsky.social View the complete gallery of OneNeuro BRAINART images here: www.oneneurojhu.org/art/ 0144
Sriram S @srirams433.bsky.social · 15/11/2025Happy #FluorescenceFriday from this pair of white-matter oligodendrocytes! 1121
Reposted by Sriram SDavid Schoppik @schoppik.com · 02/11/2025Thread coming soon, but be the first to read the latest from @franziau.bsky.social! 1278
Reposted by Sriram SXiaoyun Ding @xyding.bsky.social · 22/10/2025In the desert of tools to manipulate oligodendrocytes in the brain, we built an OASIS. 🌿 Pun intended. I’m still in awe every time I spot an amazing-looking cell. The beauty of OL and myelin never fails me! Hope this tool helps more people see it too. 0106
Sriram S @srirams433.bsky.social · 04/10/2025New cell-type for #FluorescenceFriday: astrocytes in the cerebral cortex 1151
Sriram S @srirams433.bsky.social · 19/09/2025Which track do I choose: On #FluorescenceFriday, a pair of newly formed oligodendrocytes encounter a field of neuronal axons. Later in development, they will wrap around and insulate subset of these axons 0194
Reposted by Sriram SScience Magazine @science.org · 12/09/2025The hippocampus is one of the most studied brain areas because of its major role in fundamental brain functions including learning, memory, and spatial navigation. In a new #ScienceReview, researchers focus on the role of overlooked areas and circuits within the hippocampus. scim.ag/3VKRbMM 1325
Sriram S @srirams433.bsky.social · 19/08/2025Delighted to share this work on intracortical arbor development and selective synaptogenesis by corticothalamic neurons. This was a really fun collaboration with Alan and the Brown lab during my PhD. 021
Reposted by Sriram SSangsoo Lee @sangsoolee.bsky.social · 10/08/2025The Wu Lab at @JohnsHopkins is looking for a new postdoc fellow to study sleep and/or circadian-related behaviors in mice. If you're interested in, please contact us. www.markwulab.netmarkwulab.netWu Lab 001
Reposted by Sriram SSfN Journals @sfnjournals.bsky.social · 28/07/2025#JNeurosci: @srirams433.bsky.social Guzman-Clavel et al. uncover roles for Mef2c in cortical development: regulation of laminar subtype specification during embryogenesis & axon targeting in postnatal callosal neurons. @oneneuroinitiative.bsky.social doi.org/10.1523/JNEUROSCI.0201-25.2… 022
Reposted by Sriram SJustus Kebschull @justuskebschull.bsky.social · 26/06/2025www.biorxiv.org/content/10.1...biorxiv.orgMapping the projectional architecture of the mouse midbrain dopaminergic system using cell type-specific barcodingBrain-wide neural circuits are formed by the diverse axonal branching patterns of many individual neurons. Here we introduce POINTseq (projections of interest by sequencing), a high-throughput and use... 083
Reposted by Sriram Sstanfordbrain.bsky.social @stanfordbrain.bsky.social · 23/06/2025Stanford research by Dr Barres changed our understanding of myelin—once seen as static insulation, now linked to learning & memory. Learn how @michellemonje.bsky.social, @erinmgibson.bsky.social, @bradzuchero.bsky.social & others continue to shape the field: neuroscience.stanford.edu/news/underst... 0103
Sriram S @srirams433.bsky.social · 25/05/2025Thank you so much! Really nice to hear you enjoyed the paprr 000
Sriram S @srirams433.bsky.social · 24/05/2025If you're interested in how Mef2c tunes neuronal response to repulsive guidance cues in the cortex, here's a link to the paper: www.jneurosci.org/content/45/2...jneurosci.orgMef2c Controls Postnatal Callosal Axon Targeting by Regulating Sensitivity to Ephrin RepulsionIntracortical circuits, including long-range callosal projections, are crucial for information processing. The development of neuronal connectivity in the cerebral cortex is contingent on ordered emer... 021
Sriram S @srirams433.bsky.social · 24/05/2025Celebratory #FluorescenceFriday this week, with the JNeurosci cover featuring an image from our article on how Mef2c controls intracortical projection targeting 2151
Reposted by Sriram SOneNeuro Initiative @oneneurojhu.org · 02/05/2025The winner of OneNeuro's April #FotoFriday #FluorescenceFriday contest was submitted by Luis Guzman-Clavel at the Alex Kolodkin Lab. For more information, see pubmed.ncbi.nlm.nih.gov/40228894/ View the complete gallery of OneNeuro BRAINART images here: www.oneneurojhu.org/art/ 0203
Reposted by Sriram SSeth Blackshaw @sethblackshaw.bsky.social · 29/04/2025Our latest manuscript (7 years in the works) tackles the question of how diurnal ground squirrels evolved a cone-dominant retina, in contrast to the ancestral rod-dominant retina retained by virtually all other mammals./1 www.biorxiv.org/content/10.1...biorxiv.orgHeterochronic transcription factor expression drives cone-dominant retina development in 13-lined ground squirrels.Evolutionary adaptation to diurnal vision in ground squirrels has led to the development of a cone-dominant retina, in stark contrast to the rod-dominant retinas of most mammals. The molecular mechani... 68531
Reposted by Sriram SMark Peifer (He, him) @peiferlabunc.bsky.social · 16/04/2025Our field has lost an amazing scientist, mentor, and old friend. Benny, along with Mike Hoffman, had a brilliant idea in the early 1980s. The first oncogenes were being cloned and they realized if we wanted to understand their roles in cancer, we needed to understand their normal function 1/n 🧪 26428
Reposted by Sriram SMehmet Keles @flyman.bsky.social · 08/04/2025I am proud to share that our work of 4+ years has finally been published. We wondered if there are distinct behaviors that mark sleep in 🪰, where sleep has been defined as prolonged immobility. 🔗 www.science.org/doi/10.1126/...science.orgFlyVISTA, an integrated machine learning platform for deep phenotyping of sleep in DrosophilaA machine learning platform identifies and characterizes the dynamics of microbehaviors during fly sleep. 23513
Sriram S @srirams433.bsky.social · 14/03/2025Bittersweet #FluorescenceFriday saying goodbye to the Kolodkin lab with one last image, this time from the retinal side side of the lab. Here's a collection of retinorecipient targets in the mouse brain. How many of them can you name? 092
Reposted by Sriram SNicolas Renier @nicolasrenier.bsky.social · 07/02/2025We're organizing the 4th iDISCO / TRISCO workshop at @rockefelleruniv.bsky.social in NYC! April 14-19 - FREE - registration to kavlifellows@mail.rockefeller.edu. Deadline: March 1st. We'll talk about brain mapping of cells, genes, axons, vasculature, light sheet microscopy, tissue clearing. 042
Sriram S @srirams433.bsky.social · 01/02/2025'From thought to action'... axons from the sensory cortex (green) arrive in the motor region to synapse with target neurons (yellow). Can't wait to get back to more imaging after weeks of thesis writing #FluorescenceFriday 1161
Sriram S @srirams433.bsky.social · 24/01/2025Very grateful to my advisor, Alex, for the support and freedom that made this study possible. And for Luis, first as a summer intern and now as a Kolodkin lab technician, for driving this forward.(12/n) 000
Sriram S @srirams433.bsky.social · 24/01/2025Mef2c loss and disrupted interhemispheric connectivity are both associated with neurodevelopmental and neuropsychiatric conditions. We find a functional link between Mef2c control of an axon guidance gene, EphA6, and the topography of interhemispheric connectivity (11/n) 100
Sriram S @srirams433.bsky.social · 24/01/2025Also, reducing the levels of repulsive ligand EfnA5 in the opposite (target) hemisphere restored targeting by Mef2c mutant neurons (10/n) 100
Sriram S @srirams433.bsky.social · 24/01/2025Overexpressing EphA6 in otherwise normal callosal neurons also reduces their contralateral targeting; disrupting EphA6 signaling partly restores targeting by Mef2c mutant neurons (9/n) 100
Sriram S @srirams433.bsky.social · 24/01/2025We reasoned that Mef2c mutants fail to project to the opposite somatosensory cortex because of this misregulation of EphA receptor expression (8/n). 100
Sriram S @srirams433.bsky.social · 24/01/2025Looking at transcriptional profiling from Christopher Cowan’s lab, we were struck by the upregulation of EphA repulsive guidance receptors upon Mef2c loss. The somatosensory cortex has high levels of repulsive ligand EphrinA5, compared to more medial regions. (7/n) 100
Sriram S @srirams433.bsky.social · 24/01/2025Curiously, initial axon growth into the corpus callosum and midline crossing are unaffected. The target innervation step is specifically compromised. (6/n) 100
Sriram S @srirams433.bsky.social · 24/01/2025Instead, their projections are shifted to the more medial cingulate cortex. Projection to other targets is also disrupted.(4/n) 100
Sriram S @srirams433.bsky.social · 24/01/2025Deleting Mef2c specifically in callosal neurons of the somatosensory area in one cortical hemisphere compromises their ability to connect to the somatosensory cortex on the opposite side.(4/n) 100
Sriram S @srirams433.bsky.social · 24/01/2025Mef2c is highly expressed in callosal neurons, which connect the two hemispheres of the cerebral cortex, just as they’re starting to elaborate their axonal projections.(3/n) 100
Sriram S @srirams433.bsky.social · 24/01/2025Here's the link: www.biorxiv.org/content/10.1... (2/n)biorxiv.orgMef2c Controls Postnatal Callosal Axon Targeting by Regulating Sensitivity to Ephrin RepulsionCortical connectivity is contingent on ordered emergence of neuron subtypes followed by the formation of subtype-specific axon projections. Intracortical circuits, including long-range callosal projec... 110
Sriram S @srirams433.bsky.social · 24/01/2025Happy to share our pre-print, “ Mef2c controls postnatal callosal axon targeting by regulating sensitivity to Ephrin Repulsion”. We found that the topography of interhemispheric connectivity in the cerebral cortex is disrupted upon Mef2c deletion.(1/n) 200