iiinova — where your digital self keeps evolving

Reposted by Søren Grubb

Sasha (Aleksandra) Petrovskaya @sashapetrovskaia.bsky.social · 16/08/2026

We’re back after a great @naddenmark.bsky.social workshop, devoted to brain barriers & fluids, and headache. It broadened my knowledge about migraine targets beyond CGRP, barriers beyond the BBB and “good” sides of CSD…Thank you - @chrstaehr.bsky.social @sorengrubb.bsky.social and other organizers!

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Reposted by Søren Grubb

Mill_lab @cilialab.bsky.social · 13/01/2026

Double PI act @sorengrubb.bsky.social @amreenmughal.bsky.social continuing in brains with #cilia & #centrosome diversity across microvascular system, with striking species differences on cilia metrics & directionality of spaces sampled. Deep read: www.biorxiv.org/content/10.1... #UKCilia 5/8

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Søren Grubb @sorengrubb.bsky.social · 12/01/2026

Want to know about our latest research on #primarycilia and #centrosomes in #brain #microvasculature? Join this e-symposium tomorrow! 🧪

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Søren Grubb @sorengrubb.bsky.social · 16/12/2025

Thanks for a great 2025!🎆🥳🎉 We’re proud to share highlights from this year at Neuroscience Academy Denmark (NAD) – showcasing the incredible work of our PhD students. 👩‍🎓👨‍🎓 A big thank you to @lundbeckfonden.bsky.social for supporting our mission. 🙏 #Neuroscience #PhD #Research #Collaboration 🧪

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Søren Grubb @sorengrubb.bsky.social · 20/11/2025

Also thanks to @helenelab.bsky.social for access to the CA1 dataset!

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Søren Grubb @sorengrubb.bsky.social · 20/11/2025

🧩 Summary: ✅ #Pericytes have #PrimaryCilia with an arterio-venular gradient🏳️‍🌈 ✅ 🚶‍♀️🧠 ECs have primary #cilia, 🐁🧠 ECs don’t ✅ EC polarity aligns opposite to #BloodFlow 🧭 ❔Lots of open questions about cilia’s role in #NeurovascularCoupling! Read the full preprint: 🔗 www.biorxiv.org/content/10.1... 🧪🧵8/8

Graphical abstract showing gradient distribution of primary cilia in the mural cells. Primary cilium frequency and lengths are reversely proportional to smooth-muscle actin expression.

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Søren Grubb @sorengrubb.bsky.social · 20/11/2025

🧭 #EndothelialCells are polarized against #BloodFlow direction ⤴️. #Centrioles are consistently upstream of the #nucleus. This polarity persists even in fixed tissue and could serve as a structural marker for flow direction. 📌 🧪🧵7/8

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Søren Grubb @sorengrubb.bsky.social · 20/11/2025

😮Surprise: 🚶‍♀️🧠 #EndothelialCells (EC) have #PrimaryCilia facing the vessel lumen, but 🐁🧠 ECs don’t. ~18% of 🚶‍♀️🧠 ECs are ciliated, mostly in arteriolar-end capillaries. What do these #cilia sense in the 🩸? 🧪🧵6/8

A schematic illustration of the neurovascular unit highlighting primary cilia in ECs.

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Søren Grubb @sorengrubb.bsky.social · 20/11/2025

In 🐁, some #pericyte #PrimaryCilia penetrate #astrocytic #endfeet and reach the #neuropil - sometimes near #synapses! Could these #cilia act as sensory “antennae” 📡for #NeurovascularCoupling? 🧪🧵5/8

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Søren Grubb @sorengrubb.bsky.social · 20/11/2025

In 🐁🧠, #ultrastructure shows that #pericyte #cilia are often ensheathed by #astrocytic #endfeet. In 🚶‍♀️🧠, #astrocytes are swollen and cilia stay confined within the basal lamina. What are they sensing from the astrocytes? 🧪🧵4/8

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Søren Grubb @sorengrubb.bsky.social · 20/11/2025

🗺️We mapped #MuralCells — including #pericytes and #smoothmuscle — as vectors➡️ to visualize where #PrimaryCilia🌟 occur across the 🐁🧠 #microvasculature. The result? A striking pattern: #cilia are abundant on #capillaries and #venules, but almost absent on #arterioles. 🧪🧵3/8

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Søren Grubb @sorengrubb.bsky.social · 20/11/2025

📊 #Pericytes are frequently ciliated in both 🐁 and 🚶‍♀️🧠—but mostly near #venules and venous-end #capillaries. Venular mural cells: up to 90% ciliated in 🐁 datasets! Arteriolar mural cells: almost none. Why this gradient? Flow, pressure, permeability? 🧪🧵2/8

(d) Percent distribution of primary cilia in MCs after microvascular stratification into pial and parenchymal vessels, including 1-3rd order capillaries from the MICrONS (mouse visual cortex), CA1 (mouse hippocampus), and h01 (human cortex) datasets. Note that neither CA1 nor h01 contain any pial vessels. (e) Lengths of MC primary cilia in pial and parenchymal vessels, including 1-3rd order capillaries MCs from MICrONS.

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Søren Grubb @sorengrubb.bsky.social · 20/11/2025

New on bioRxiv! 🚨 “Pericyte and Endothelial Primary Cilia and Centrioles have Disparate Organization Across the Brain Microvasculature” This project studying #microvascular cells took an unexpected turn when we discovered that #pericytes have primary #cilia! 🔗 www.biorxiv.org/content/10.1... 🧪 🧵1/8

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Søren Grubb @sorengrubb.bsky.social · 14/11/2025

🎥 NAD 2025 Annual Meeting Highlights From Oct 3–5, Neuroscience Academy Denmark hosted: ✅ 400+ participants ✅ 100 posters ✅ Keynotes on precision psychiatry, brain barriers, ultrasound treatments, brain stimulation, sleep & circadian rhythms We look forward to next year! #Neuroscience #NAD2025 🧪

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Søren Grubb @sorengrubb.bsky.social · 27/09/2025

That reminds me of Thor fighting “midgårdsormen” the world serpent 🤣

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Reposted by Søren Grubb

Ghouly Hoye 👻🇨🇦 @hollyhoye.bsky.social · 08/09/2025

“Well I don’t like that study because it doesn't give me the result I want’

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Reposted by Søren Grubb

Hajime Hirase @hajimehirase.bsky.social · 02/07/2025

We are excited to report: Cerebral blood flow is modulated by astrocytic cAMP elevation independently of IP3R2-mediated Ca2+ signaling in mice Vittani et al. PNAS (2025) pnas.org/doi/10.1073/...

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Søren Grubb @sorengrubb.bsky.social · 24/06/2025

The first Master’s student with me as main supervisor graduated today! I’m proud 🥹

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Søren Grubb @sorengrubb.bsky.social · 10/06/2025

In older mice, where #MyogenicTone is weaker, #PrecapillarySphincters become even more critical barriers against #BloodPressure surges—compensating for #VascularStiffening and remodeling.

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Søren Grubb @sorengrubb.bsky.social · 10/06/2025

These changes suggest that #PrecapillarySphincters protect the capillaries 🛡️ by acting as a dynamic #resistance that regulates #capillary #BloodFlow and #BloodPressure.

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Søren Grubb @sorengrubb.bsky.social · 10/06/2025

Using #InVivo #VolumeImaging, we found #age related #vascular changes: • ↑ #Tortuosity of #PenetratingArterioles • ↓ #Arteriolar-capillary density • ↑ #Capillaries at the venous end + #aneurysm-like bulbs downstream of #PrecapillarySphincters.

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Søren Grubb @sorengrubb.bsky.social · 10/06/2025

By recording #cortical #angiograms during i.v. injection of FITC-dextran 🟢 in #adult and #old #NG2DsRed mice, we time-colored the #arterioles 🔴 and #venules 🔵. We found that #OldMice have less #collaterals and more #tortuous #PenetratingArterioles.

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Søren Grubb @sorengrubb.bsky.social · 10/06/2025

After #LaserAblating pericytes around #PrecapillarySphincters, we observed impaired #MyogenicResponse and #NeurovascularCoupling—demonstrating #PrecapillarySphincter pericyte contractility is essential.

Figure showing how we used the two-photon laser to ablate the pericyte around the precapillary sphincter.

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Søren Grubb @sorengrubb.bsky.social · 10/06/2025

#OldMice also showed decreased pulsations of microvessel diameters measured by transverse line scans, likely due to #VascularStiffening.

Figure showing how we measured microvascular diameters by transverse line scanning to assess the diameter pulsatility and the pulsatility of the centers of the vessels.

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Søren Grubb @sorengrubb.bsky.social · 10/06/2025

In aged mice, the #MyogenicResponse was significantly blunted. This was shown by an increased #CorrelationCoefficient (CC) between #VascularDiameter and #BloodPressure

Figure showing blood pressure increase by the acute hypertensive challenge along with changed microvascular diameter in adult and old mice. CC= 0.13 for the adult mouse and 0.57 for the old mouse.

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Søren Grubb @sorengrubb.bsky.social · 10/06/2025

We challenged mice with acute #BloodPressure spikes (~70% increase via angiotensin II). Result: both adult and #old mice got their #MyogenicResponse and #NeurovascularCoupling disrupted

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Søren Grubb @sorengrubb.bsky.social · 10/06/2025

To visualize this, we used #InVivo #TwoPhotonMicroscopy in anesthetized adult and aged mice. Watch this video showing PS dilation in response to “SensoryStimulation! 🔬👇

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Søren Grubb @sorengrubb.bsky.social · 10/06/2025

🐭🧠 #PrecapillarySphincters (PS) at the #arteriole–#capillary junction rapidly dilate ~30% in seconds during #SensoryStimulation—regulating #capillary #BloodFlow dynamically. Here we investigated whether they protect downstream microvessels during abrupt #BloodPressure surges 🧪🧵

Showing how a precapillary sphincter dilates during high blood pressure

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Søren Grubb @sorengrubb.bsky.social · 02/05/2025

I was using indirect sunlight, but it seems like a better idea with an LED source. I was thinking of 3D printing a holder for my phone, so I don’t have to hold it at the right angle and distance to the eyepiece.

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Søren Grubb @sorengrubb.bsky.social · 02/05/2025

Playing around with my 3yo daughter and an antique microscope. Looked at some #dandelion petals and a stamen using a Carl Zeiss P.A. 35 microscope using reflected light. 🧪

Dandelion petal under a Carl Zeiss P.A. 35 microscope

Dandelion stamen under a Carl Zeiss P.A. 35 microscope

Pollen on the dandelion stamen under a Carl Zeiss P.A. 35 microscope

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Søren Grubb @sorengrubb.bsky.social · 02/05/2025

@pkpd-babe.bsky.social Can I be added to the science post? My orcID: orcid.org/0000-0002-67...

orcid.org

ORCID

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Søren Grubb @sorengrubb.bsky.social · 27/04/2025

Small world. I studied biology with one of the researchers cited in that article. 😊

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Søren Grubb @sorengrubb.bsky.social · 19/03/2025

Amazing work out by Hirase Lab 🤩

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Søren Grubb @sorengrubb.bsky.social · 18/03/2025

Join our #PhDcourse on #openscience datasets and tools in #Neurophysiology! We'll dive into #EM, #inVivo #physiology, behavioural analysis tools, and a talk on creating animations from open data! @quorumetrix.bsky.social 📢 Free for NorDoc PhD students, but open to all! Apply now! lnkd.in/dU_fxqqu

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Reposted by Søren Grubb

Eric Topol @erictopol.bsky.social · 12/01/2025

Publications in the past week built substantially on our knowledge of the brain's waste disposal system—glymphatics—and the implications on sleep and brain aging. Featuring exceptional work by Nedergaard Lab and @jonykipnis.bsky.social erictopol.substack.com/p/our-sleep-... open-access

erictopol.substack.com

Our Sleep, Brain Aging, and Waste Clearance

How sleep prevents "dirty" brains that age faster

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Reposted by Søren Grubb

Asaf (Klaf) Weisman @asafklaf.bsky.social · 25/12/2024

🤘🏻The study I have been waiting for 30 years! Only Scandinavians can pull this out let alone publish it in a Scandinavian pain journal 🤘🏻 #painscience #physio #painresearch #ignobel2025 www.degruyter.com/document/doi...

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Altmetric @altmetric.com · 11/12/2024

SERVICE UPDATE We normally don't ask you to "share like and subscribe" but please distribute this. You are now able to delete the link after it embeds on your post so you can get those precious extra characters, and we will catch your post mentioning research. #AcademicSky #Medsky #HigherEd

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Søren Grubb @sorengrubb.bsky.social · 07/12/2024

Hi Natalia, I do volume EM of macrophages and lymphocytes in the meningeal layers. Can I be added?

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Søren Grubb @sorengrubb.bsky.social · 04/12/2024

PhDs and postdocs in immunology: go.bsky.app/RABVXY4

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Reposted by Søren Grubb

Andrew Payne @andrewcpayne.bsky.social · 03/12/2024

🧪 E11 Bio is excited to share a major step towards brain mapping at 100x lower cost, making whole-brain connectomics at human & mouse scale feasible (🧠→🔬→💻). Critical for curing brain disorders, building human-like AI systems, and even simulating human brains. Read more: e11.bio/news/roadmap

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Søren Grubb @sorengrubb.bsky.social · 03/12/2024

Great! You have been added 😊

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