iiinova — where your digital self keeps evolving

Siegenthaler Lab @siegenthalerlab.bsky.social · 03/06/2026

Yep, that is much better...I can see...looking forward to testing them out on the mouse ear tags tomorrow! 🐭

Siegenthaler Lab @siegenthalerlab.bsky.social · 18/05/2026

It's graduation day @cuanschutz.bsky.social, the weather is terrible by there is celebration (indoors!) for our graduates all over campus - honored to 'hood' my 5th PhD student, the amazing Dr. Sophia Kim! 🎉 Plus a little pomp & circumstance for our 'honorary' doctor in the lab, Dr. Bones 💀❤️

two women wearing graduation regalia (hats, hoods and black robes), smiling while facing the camera

plastic skeleton wearing sunglasses and graduation robe, hat and hood - skeleton is standing between a women wearing jeans and flowery shirt and leaning on a black laboratory bench

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Siegenthaler Lab @siegenthalerlab.bsky.social · 06/05/2026

Rockies Front Range snow update: half a dog leg of snow (1 Clark dog leg=~12in, he's a big boy)...still coming down! Lovely Mother Nature coming through for us here in Colorado this spring. ❤️❄️🗻

picture of a dog on a outdoor porch with lots of snow on rails and ground, snow covered trees and fences in the background

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Siegenthaler Lab @siegenthalerlab.bsky.social · 17/04/2026

I say 'good morning' to Dr. Daly & Dr. Maud Menton everyday, these great prints from @minouette.bsky.social hang outside my office door, along with Dr. Irene Uchida & Dr. Janaki Ammal - all impactful women scientists, their pictures & stories are a source of daily inspiration for me. ❤️

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Siegenthaler Lab @siegenthalerlab.bsky.social · 10/04/2026

#FluorescenceFriday - experiments take so long (especially for PIs that moonlight as postdocs), between breeding, tamoxifen, waiting for the 🐭 to grow up, slicing, clearing, staining for CD206 then finding a chunk of time on the 🔬...but then I get to image all my fav cells & it's SO worth it. ❤️

Postnatal day 24 mouse cerebral cortex - Col1a2-CreERT; tdTom (red) to label firbroblasts, perfused with lectin (white) and macrophages (CD206) in blue - these are penetrating arterioles covered by perivascular fibroblasts and macrophages, smaller vessels in the brain are not

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Siegenthaler Lab @siegenthalerlab.bsky.social · 07/04/2026

Congrats to the newest PhD from the @siegenthalerlab.bsky.social, Dr. Sophia Kim! Beautiful thesis document & presentation! 🧠🦠🔬🎉 Sophia's thesis work on arachnoid barrier breakdown in meningitis is on bioRxiv - check it out! www.biorxiv.org/content/10.6...

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Siegenthaler Lab @siegenthalerlab.bsky.social · 07/04/2026

I did well in my Men's NCAA basketball bracket this year, picked the Final 4 & winner Michigan. Turns out picking teams because 'there's good science being done there' or 'I like their mascot' or 'they invited me for a talk' or 'my good friend went to school there' is not a bad way to do it. 🏀🔬🧪🧫🧠

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Siegenthaler Lab @siegenthalerlab.bsky.social · 26/03/2026

🤩 new review on CNS perivascular fibroblasts interactions in injury, disease & neuroimmunity - from Natalia Barbara Pikor group @ethz.ch led by Margaux Océane Verdon - check it out! academic.oup.com/jimmunol/art...

Fibroblasts underpin immune-competent niches in the healthy and diseased CNS. Schematic representation of the topological organization of fibroblasts along the vascular tree and across meningeal and brain parenchymal compartments in the context of (A, B) homeostasis, (C, D) infection, (E) autoimmunity, and (F) sterile tissue injury. The provision of cytokines, chemokines, survival factors, and ECM components by local fibroblasts are indicated in each condition. The legend of depicted cell types and vasculature is provided. (A) In the homeostatic CNS, fibroblastic cells include mural cells, perivascular fibroblasts situated abluminal to mural cells, and meningeal fibroblasts. (B) In the steady-state condition, PDPN+ FRC-like cells are limited to DALTs positioned at the intersect of rostral-rhinal, olfactory, and superior sagittal sinuses. In (C) chronic dural infection and (E) autoimmunity, persistent inflammation is associated with TLS formation. TLS are underpinned by FRC-like cells and FDCs providing T and B cell chemoattractants, survival factors, and canonical markers of FRCs. (D) In infections of the brain parenchyma, similar chemokines and FRC markers are upregulated in the absence of TLS formation. (E) In the context of deep gray matter injury, perivascular fibroblasts of the Virchow-Robin space constitute a major source of scar-forming cells. In addition to profuse ECM deposition, scar-forming fibroblasts secrete myeloid cell survival factors and chemokines to drive early and late phase scarring, respectively.

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Siegenthaler Lab @siegenthalerlab.bsky.social · 18/03/2026

In grad school, there was this old professor that sat in the 1st row & ALWAYS asked questions at EVERY talk, didn't matter the topic. Drove us students nuts but was also endearing, he just loved science. That's me, at EVERY talk...now with the title. Professor Siegenthaler, effective 7/1/2026.

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Siegenthaler Lab @siegenthalerlab.bsky.social · 06/03/2026

#FreehandFigureFriday continues...this from a figure I mocked up for a review but ended up on the 'digital' cutting room floor - scanning EM of the meninges of large mammals like dogs (this from PMID: 1094039) is really great, gives a great appreciate for 'smoothness' of SAS surfaces.

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Siegenthaler Lab @siegenthalerlab.bsky.social · 06/03/2026

Poking around files for a diagram to share with a colleague and came across these graphics I had drawn of the meninges (I think these are in a chapter from several years ago) - not a #FluorescenceFriday but rather #FreehandFigureFriday for bespoke scientific schematics ✍️

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Siegenthaler Lab @siegenthalerlab.bsky.social · 06/03/2026

This is our team! Sophia, Kelly, Amanda, Brady & Luke - super great people and a great collaboration w/ the Doran lab! 4/5

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Siegenthaler Lab @siegenthalerlab.bsky.social · 06/03/2026

Sophia did an AMAZING job with this project - big props for innovating on 🔬 & image analysis - the first figure of this paper took a bit because she had to develop tools to study arachnoid barrier functional integrity in pups & quantify Cldn11 junctions [we couldn't just say 'they look weird' 😂] 3/5

Figure 1. Neonatal arachnoid barrier is disrupted during GBS infection. (A) Schematic of arachnoid barrier integrity assay (ABIA) using a plate reader and thick section imaging (adapted from Zhang et al., 2022). (B) Plate reader quantification of % Biocytin-TMR fluorescence in the superficial cortex (cortical fluorescence normalized to LPM fluorescence). (n=4 mock, n=9 GBS) (C) Visualization of Biocytin-TMR in the leptomeninges (LPM) and cortex between mock-infected and GBS-infected group (500µm coronal brain sections). (D) Representative images of E-cadherin+ (E-cad) and Claudin-11+ (Cldn11) AB junctions on LPM whole mounts using immunofluorescence (IF). Asterisks indicate locations of Cldn11+ junction aberrance. (E) Line intensity analysis of E-cad+ and Cldn11+ junctions (peak at ±0-0.45µm) and non-junctions (between ±2-3µm) on LPM whole mounts. (F) Ridge Detection analysis of Cldn11+ junctions (left) and quantification (right) on LPM whole mounts. (n=7 mock, n=8 GBS for 1E and 1F) Statistics: Mann-Whitney U test, * = p < 0.05, ns = not significant (p-value > 0.05); mean and SD. Scale bars = (C) 200µm, (D) 20µm.

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Siegenthaler Lab @siegenthalerlab.bsky.social · 06/03/2026

🚨 New preprint from our lab led by Sophia Kim w/ @kellydoran.bsky.social group @cuanschutz.bsky.social -- using neonatal 🐭 model of bacterial meningitis ➡️ arachnoid barrier is leaky, Cldn11 junctions are disrupted & cytokines are part of the problem. Lots of 🤩🧠🦠🔬 & compelling data, check it out! 1/5

graphical schematic of the meninges on top depicting the three layers [dura, arachnoid and pia] and other immune and vascular cell types. Inset shows Claudin-11 junction proteins connecting two sides of arachnoid barrier cells

bottom schematic is the same but in inflammation with bacteria, junctions breakdown, depiction of cytokine release by vascular, meningeal and immune cells - at side of graphic is summary of defects we observe in infection: 'increased barrier permeability' 'mislocalization of Cldn11+ tight junction' 'BAM activation' and "increased inflammatory response'

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Siegenthaler Lab @siegenthalerlab.bsky.social · 18/02/2026

Good science-ing this morning making figures & editing manuscript drafts, good hair day 👱🏼‍♀️, my office is filled with Colorado sunshine & my office orchids have bloomed...I'll appreciate all the little 'wins' that come my way. 😊👩‍🔬🌸📊🧠

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Siegenthaler Lab @siegenthalerlab.bsky.social · 16/02/2026

"Biomedical and life science articles by female researchers spend longer under review" from @plosbiology.org. After I read this sentence, I counted & I have 43 papers w/ a female first and/or last author. Lots of time wasted in longer reviews/revisions. 😕 journals.plos.org/plosbiology/...

Even though, for each manuscript, time under review is only 7–15 days longer for female- than for male-authored articles (Fig 2), accumulated over a woman’s career, these delays can be very substantial. For instance, for every 50 papers published by a female author, she will have spent on average 350–750 days longer than her male counterparts waiting for reviews and editorial decisions and/or revising manuscripts.

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Siegenthaler Lab @siegenthalerlab.bsky.social · 09/02/2026

Pial cell junctions with ZO-1 (green) and beta-catenin (magenta) with a few pial blood vessels at the edges (adult mouse) - the sample prep for this is tough but worth it to see these lovely cell connections. #microscopymonday

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Siegenthaler Lab @siegenthalerlab.bsky.social · 18/01/2026

Thanks for starting this Harmit, ❤️ reading how proactive folks are being. No great answers either, there is no easy fix. Lately...'state of the lab' meeting to talk about our accomplishments, setting yearly goals, how we will get there, be open to talk about ANYTHING...more swearing in lab, too. 🥴

picture of raccoon between a trash bin and toilet

picture of store with bottles on shelves and broken bottles on the floor

screen shot of newspaper title that reads "Raccoon goes on a drunken rampage in Virginia liquor store and passes out on bathroom floor"

collage of pictures of groups of people with titles above some pictures

titles read "Axe throwing...goodbye Christina!" "Colorado Rockies with the Bonney lab" "Ash & Danae passed comps" "Goodbye Hannah"

What did we do in 2025? Publications – 1 preprint, 3 research papers, 2 spotlight articles

Christina, Danae with Franco lab – “Meningeal-derived retinoic acid regulates neurogenesis via suppression of Notch and Sox2” – Published in Cell Reports

Hannah - In collab with Gama Lab @ Vanderbilt – “Leptomeningeal Neural Organoid (LMNO) Fusions as Models to Study Meninges-Brain Signaling” – Published in Stems Cells and Development

Sophia – spotlight article “The VIP of the leptomeninges” – published in Neuron

Danae – spotlight article ”Fibroblasts are not so scar-y in brain injury” published in Immunity

Hannah, Kelsey, Sophia – “PDGFr-alpha is required for postnatal cerebral perivascular fibroblast development” preprint and published in Developmental Biology

2026 Goals:grants & manuscripts

Grants
Pending grants
AB R01 renewal - potential submission Summer (pending review in Spring, 2026)
PDGF-PVF R01 grant – potential resubmission Summer (pending review in Jan, 2026)
Co-PI grant with Franco lab – R21 – potential resubmission Summer (pending review March, 2026)
F31 – potential resubmission April, August (pending review Spring, 2026)
New grants
Co-PI for Santos’ R01 renewal – have an aim on retinoic acid regulation of glial development (Ash, Julie)
Open to new ideas…
Manuscripts
Meningitis – Arachnoid Barrier breakdown (Sophia, Brad w/ Doran lab) – late Winter, 2026
PDGF signaling meninges – primary research article or review (Danae, Julie) – April, 2026
Foxc1 conditional deletion [Wnt1-cre, Tbx18-cre – Foxc1-flox alleles + E13/E16 single cell] – Summer, 2026 – (Brad, Julie, Danae, Emily)
Fibroblast regulation of BAM development – Brad, Elizabeth – Summer-Fall, 2026
Cadherin regulation of Arachnoid Barrier development/function – (Sophia, Brad, Julie) – Fall-Winter, 2026

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Siegenthaler Lab @siegenthalerlab.bsky.social · 25/12/2025

My doggo Clark 'asked' for a hike for his Christmas 🎁...who am I to refuse? I hope everyone is having a relaxing, joy-filled day! ❤️

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Siegenthaler Lab @siegenthalerlab.bsky.social · 23/12/2025

Regal beast...West of the Mississippi edition

yellow dog wearing a harness standing on a dirt trail, rocks and mountains with trees in the background

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Siegenthaler Lab @siegenthalerlab.bsky.social · 01/12/2025

Had a nice holiday weekend, celebrated my birthday early with dinner & holiday lights while our kiddo was 🏡 for break - having your kid home after sending them off to college is a feeling like none other...pure joy! ❤️

Picture of a women and young man smiling, in the background is trees with string lights wrapped around the tree trunks

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Siegenthaler Lab @siegenthalerlab.bsky.social · 20/11/2025

Our work out in @devbiol.bsky.social, PDGFra is needed for 🧠 perivascular fibroblast development... ⬇️ perivascular fibroblast = ⬇️ perivascular macrophage...cross-talk? 👀 Led by Hannah Jones @hejcell.bsky.social w/ Kelsey Abrams, Sophia Kim, excited for more projects w/ @fantauzzolab.bsky.social!

Micrscopy images of cleared cerebral brain tissue showing penetrating blood vessels (white) in mice - magenta cells are perivascular fibroblasts and cyan cells are perivascular macrophages - in control mice, both cell types are near the vessel termini by postnatal day 10 but in 'cKO' mice that lack expression of a tyrosine kinase receptor PDGFRa in fibroblasts, both the fibroblasts and macrophages are only partially covering the vessels. From Figure 4 of our paper - https://authors.elsevier.com/a/1m7fa2mzTkyfS

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Siegenthaler Lab @siegenthalerlab.bsky.social · 18/11/2025

New headshot for the upcoming new year...💫

picture of a woman facing the camera and smiling, in the background is scientific lab with glass bottles on the shelves

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Siegenthaler Lab @siegenthalerlab.bsky.social · 31/10/2025

Last big grant of 2025 submitted today...Dr. Bones [channeling Slash here on Halloween] says it all for me. 💀🧠💰🎃

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Siegenthaler Lab @siegenthalerlab.bsky.social · 28/10/2025

Had our 1st joint lab fun activity with the Bonney Lab @stephaniebonney.bsky.social - a pumpkin painting party! 🎃 So creative! Works well with our 4th floor Halloween activity of 'guess the 🧠 pumpkin's weight' ⚖️...🧠 pumpkin now has a vasculature complete w/ perivascular fibroblasts & macrophages!

pumpkin sitting on a table, red and blue paint depicting vasculature on the pumpkin surface - signs pinned above the pumpkin say "GUESS THE BRAIN PUMPKIN'S WEIGHT"

four small pumpkins painted with a moon and clouds, ghosts, owl and fuzzy black characters with eyes

two small pumpkins - pumpkin at left is black with cat face and pumpkin at right is back with a mouse face

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Siegenthaler Lab @siegenthalerlab.bsky.social · 20/10/2025

For all those who are WFH today, riding the struggle-bus through a mid-October, not-enough-time-left-in-the-year-and-things-feel/are-crappy Monday 🙋‍♀️...take a DEEP breath &, if possible, pet your animal...get a nice, deep scratch in!

picture of yellow lab dog looking up while person's hand scratches behind the dog's right ear

picture of yellow lab dog yawning and getting scratched behind right ear

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Siegenthaler Lab @siegenthalerlab.bsky.social · 11/10/2025

My mood this morning... Reviewing my 3mo science goals going into mid-October, ✅ boxes off & looking at this picture that accurately describes me ATM [the 'empty nester', me making a funny face, my boy walking out of the photo frame...from Coit Tower in SF this summer].

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Siegenthaler Lab @siegenthalerlab.bsky.social · 03/10/2025

Listening to the new Taylor Swift album & working on figures, sharing a 🤩 image for #FluorescenceFriday [penetrating 🐭 cerebral arteriole at the pial surface, aSMA in yellow = smooth muscle cell, pan-laminin in cyan = basement membrane, fibroblast in magenta...squeezing in to cover that vessel 🫂)

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Siegenthaler Lab @siegenthalerlab.bsky.social · 01/10/2025

Had a 🤩 time in Freiburg 🇩🇪 for the CNS Wound Healing & Scarring meeting organized by Jae Lee @univmiami.bsky.social & Christian Schachtrup @uni-freiburg.de - amazing progress in this field! ⚡🧠🩹 Took a short trip to Basel & Luzern 🇨🇭 to finally connect with my Swiss roots...my ❤️ of ⛰️ & 🥾 is in my 🧬

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Siegenthaler Lab @siegenthalerlab.bsky.social · 19/09/2025

We login with our university ID onto the imaging analysis computer & I always laugh being greeted by 'younger Julie' in my first faculty photo 😂 (**I am apparently am THAT kind of faculty that does not update their photo though I now understand why this happens...who has time for that?!?!)

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Siegenthaler Lab @siegenthalerlab.bsky.social · 01/09/2025

I ❤️ dahlias, the bigger the better! Happy birthday! I hope you have a great day. 🌹🌸🌼

picture of a dahlias (a type of flower) in bloom, orange, pink and red colors

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Siegenthaler Lab @siegenthalerlab.bsky.social · 29/08/2025

It was a great day @cuanschutz.bsky.social for the @siegenthalerlab.bsky.social - our grad student Danae Mitchell gave an amazing talk & passed her comprehensive exam 🎉...& we celebrated after by holding puppies. Congrats Danae!

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Siegenthaler Lab @siegenthalerlab.bsky.social · 24/08/2025

We were on the trails in Boulder bright & early this Sunday morning...Clark is one happy doggie (so are his humans!). 🐶+⛰️=😁

picture of yellow dog walking on a dirt trail, trees, mountains and a partly cloudy sky in the background

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Siegenthaler Lab @siegenthalerlab.bsky.social · 21/08/2025

Asking my #DevBio @socdevbio.bsky.social & #Neuroscience @sfn.org colleagues to join me in donating to the ABRCMS conference poster award fund. I am sure there will be 🤩 posters on these topics @ ABRCMS 2025 in Nov, let's make sure there is award $ to recognize their work! abrcms.org/donate/stude...

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Siegenthaler Lab @siegenthalerlab.bsky.social · 18/08/2025

The experiment didn't work as expected yet the data is still beautiful, so I got that going for me. 😜 And I spy with my little eye a mitotic figure... #MicroscopyMonday

en face view of the mouse leptomeninges depiciting the arachnoid barrier cell layer, postnatal day 3; = yellow = E-cadherin; magenta = Claudin-11, cyan = cell nuclei

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Siegenthaler Lab @siegenthalerlab.bsky.social · 10/08/2025

Clark has a had a rough couple weeks...'mom' has been too busy writing grants to hike ⛰️...he was bit by another dog on a walk [required a vet visit & staples 🥴]...BUT 'mom's' grant is submitted, wound is healed & it's a GREAT day for a long hike....made perfect with a dip in the creek at the end. ❤️

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Siegenthaler Lab @siegenthalerlab.bsky.social · 10/08/2025

Pretty certain running a lab is mostly about making signs to avoid inconveniences & minor catastrophes. 😊

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Siegenthaler Lab @siegenthalerlab.bsky.social · 07/08/2025

Submitting a R01 grant today...different R01 submitted in April has study section tomorrow.... *cue me humming, head-nodding along to Cindi Lauper's "Girls just want to have fun"...changing the last word to "funding"...because that's all we really want. Seriously.

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Siegenthaler Lab @siegenthalerlab.bsky.social · 26/07/2025

Grant due in >2 weeks = Saturday morning grant writing. Made far easier with a lovely view of bluebird Colorado skies, a freshly mowed lawn (courtesy of my boy) & ☕. 🌞⛰️👩‍💻🧠🔬🐭

Image of deck and backyard, blue skies, tree and green lawn. In foreground is partial view of a laptop with word documents on screen, white coffee cup and deck chair with white and red pillows.

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Siegenthaler Lab @siegenthalerlab.bsky.social · 17/07/2025

I am inspired by my colleagues, trainees & staff in the Dept of Pediatrics, Section of Dev Bio @cudevbio.bsky.social - studying genetic & environmental causes of congenital disorders, developmental origins of infertility, pediatric 🧠 cancer, intellectual disability & type 1 diabetes 🐭🧫🐟🧬🫀🧪👩🏻‍🔬👨🏽‍🔬

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Siegenthaler Lab @siegenthalerlab.bsky.social · 14/07/2025

Bassem Hassan @bassemh.bsky.social, Corey Harwell & yours truly are co-chairs for 2026 Neural Development #GRC August 2-7, 2026 in Newport, RI 🇺🇸 - if you are interested in updates on the conference & to hear about our 🤩 line-up of speakers...follow @neuraldevgrc.bsky.social! 🧠🪰🐭🐙🐸🦎🧒🏻🧫🧪🧠

image of web page for Neural Development GRC - go to https://www.grc.org/neural-development-conference/2026/

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Siegenthaler Lab @siegenthalerlab.bsky.social · 11/07/2025

Thanks so much to the @diffjournal.bsky.social editors for an opportunity to talk about our paper on retinoic acid in retinal vascular development! Looking forward to attending these sessions in the future AND continuing to submit our work for publication in Differentiation! 🧠 👁️🐭🔬

Image of powerpoint slide with text

“Retinoic acid signaling in mouse retina endothelial cells is required for early angiogenic growth”
Differentiation (2023)
Authors: Christina Como, Cesar Cervantes, Brad Pawlikowski and Julie Siegenthaler

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Siegenthaler Lab @siegenthalerlab.bsky.social · 27/06/2025

Next week is 13 yrs since I started at @cuanschutz.bsky.social & opened the Siegenthaler Lab...very grateful for every trainee, every colleague & collaborator, every grant/foundation $$ that contributed to our discoveries. Even now, over a decade in, I am happy to be @ the 🔬 & imaging the meninges.

picture of woman smiling, in background is a computer screen with a image depicting the mouse meninges at E13

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Siegenthaler Lab @siegenthalerlab.bsky.social · 24/04/2025

🤩 paper on CNS fibroblasts in leptomeninges medulloblastoma metastasis from Michael D. Taylor's group co-led by Namal Abeysundara, Alexandra Rasnitsyn, & Vernon Fong in @natcellbio.nature.com - more evidence that CNS fibroblasts are a 🔑 player in 🧠 tumor growth 🧪 www.nature.com/articles/s41...

Extended Data Fig. 10

In the normal leptomeninges, meningeal fibroblasts are present and associate with meningeal blood vessels. In the presence of medulloblastoma (MB) metastatic tumour cells, secreted platelet-derived growth factor (PDGFA) ligands in the local microenvironment chemotactically recruit meningeal fibroblasts. Meningeal fibroblasts are reprogrammed to become MB-MAFs, expressing distinct transcriptomes and secretomes, including bone morphogenetic proteins 4 and 7 (BMP4/7). Active BMP and PDGF signalling further enhance both the colonization of the leptomeninges by MB cells as well as promoting the growth of established metastases.

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Siegenthaler Lab @siegenthalerlab.bsky.social · 20/04/2025

Great new work led by @biomarina-vg.bsky.social on zebrafish meninges! Particularly 🤩 is the descriptions of ependymin-expressing meningeal cells (not a fibroblast!) & ablation studies showing their essential role in viability. So much more to learn! Congrats Marina & team on this great work! 🦓🐟🧠

Adult zebrafish possess complex multilayered cephalic meninges. Transmission electron micrograph (TEM) images of transverse sections through the dorsal heads of adult zebrafish, at the level of the optic tecta. A, Pseudocolored overview image of the cephalic meninges, revealing multiple tissue layers between the skull and the brain parenchyma. B, Dorsal surface of a dissected adult zebrafish brain, showing the leptomeninges partially peeling away from the underlying brain parenchyma. C, Higher magnification image of the boxed region in panel B, showing multiple leptomeningeal cell layers separated by membrane bilayers (red arrowheads), delimited ventrally by a well-defined basal lamina (pseudocolored purple). D, Nucleated red blood cell-containing blood vessel embedded within the leptomeninges, with desmosomes and junctions connecting individual endothelial cells (arrows). E, Higher magnification image of the boxed region in panel D, showing a portion of the vessel wall with a characteristic endothelial cell-cell junction (arrow). F, Higher magnification image of collagen fibers in the adult zebrafish leptomeninges. G, Pseudocolored overview image of the cephalic meninges, showing separated heavily pigment dura mater (dural meninges) and leptomeninges. multiple tissue layers (pseudocolored) between the skull and the brain parenchyma. H, Higher magnification image of the boxed region in panel G, showing bilayered dural meninges with periosteal dura adjacent to the skull with an underlying meningeal dural cell layer with numerous large melanophore pigment granules (arrows). Scale bars = 3 µm (A), 4 µm (B,G), 1 µm (C,H), 2 µm (D), 0.3 µm (E), and 0.2 µm (F).

M, Dorsal view of an adult casper zebrafish head with the optic tectum and cerebellum clearly visible through the thin, translucent skull. The yellow box shows the approximate region depicted in panels N and O. N,O, Confocal images of the meninges imaged through the intact skull of a living Tg(mrc1a:egfp)y251, Tg(epd:mcherry)y715 double transgenic adult zebrafish, showing (N) mrc1a:egfp-positive FGPs and lymphatics (green) and epd:mcherry-positive LBCs (magenta), or (O) only epd:mcherry-positive LBCs (magenta). P, High-resolution, high magnification confocal Z-stack through the leptomeninges on the surface of a dissected Tg(mrc1a:egfp)y251, Tg(epd:mcherry)y715 double transgenic adult zebrafish brain, showing LBCs (magenta) and FGPs (green). The large center image is a dorsal XY view of the reconstructed image stack, and the images below and to the right show single-plane XZ and YZ views along the planes noted by the dashed lines (xz-1, xz-2, yz).

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Siegenthaler Lab @siegenthalerlab.bsky.social · 12/04/2025

@cshlmeetings.bsky.social Brain Barriers 2025 is a wrap! It was an honor to co-organize this meeting in 2023 & 2025, a highlight of my scientific career. I am amazed by all the great science presented by these awesome scientists over the last five days...the future of CNS barrier biology is 🤩 💫!

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Siegenthaler Lab @siegenthalerlab.bsky.social · 08/04/2025

Tonight I am a tired scientist who just turned in a R01 ...tomorrow I am a proud organizer with Joy Zuchero, Caroline Menard @caromenard666.bsky.social & Ayal Ben-Zvi @ayalbenzvi.bsky.social for the @cshlmeetings.bsky.social on Brain Barriers - just the boost of 🤩 science & colleagues my ❤️ & 🧠 needs

Image of the cover of the Cold Spring Harbor Lab Program Book for Meeting on Brain Barriers 2025 - text reads Brain Barriers April 8-April 12, 2025 - image below of a large brain blood vessel in red surrounded by cells, green microglia and purple astrocytes

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Siegenthaler Lab @siegenthalerlab.bsky.social · 20/03/2025

Dr. Maud Menten is on my 👩‍🔬👩🏽‍🔬👩🏻‍🔬 in STEM Wall of Fame. When I put this together in early 2025, a Christmas gift to myself, I knew that I, & others, would need a daily reminder of their great accomplishments & perseverance in the face of difficult odds. 🙏 @minouette.bsky.social for the beautiful prints!

3-panal framed picture hanging on a grey wall of biochemist Dr. Marie Maynard Daly (left) and medical research Dr. Maud Menten (right) with text describing their accomplishments in the middle panel of the frame.

3-panal framed picture hanging on a grey wall of geneticist Dr. Irene Ayako Uchida (left) and botanist Dr. Janaki Ammal (right) with text describing their accomplishments in the middle panel of the frame.

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Siegenthaler Lab @siegenthalerlab.bsky.social · 17/03/2025

I was imaging this weekend, took this image because it was beautiful to me, the blood vessels, fibroblasts, astrocytes, & macrophages as steadfast guardians at the brain's edges. Cheers to the 'guardians' of all that is important. ❤️

microscope image of a postnatal mouse brain showing the entry point of blood vessel into the brain - fibroblasts (yellow: S100a6) form a layer at the brain's edge (meninges) and surround the blood vessel. Astrocyte endfeet (magenta, GFAP) form a layer at the brain's edge, apposed to the fibroblasts. Image also shows macrophages (cyan; CD206) and cell nuclei (DAPI, blue)

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Siegenthaler Lab @siegenthalerlab.bsky.social · 23/01/2025

🚨 CNS barriers scientists...come join us for #cshlbbb 2025! Submit your abstracts by January 24th...we pick MANY talks from abstracts - we look forward to seeing you in April!

adult mouse leptomeninges - VE-cadherin (magenta) labeling junctions between endothelial cells in brain vasculature and between pial cells - blue nuclei [DAPI]

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