Telomerase is a reverse transcriptase that synthesizes telomeric repeats at chromosome ends, safeguarding genome integrity. We present the cryo–electron microscopy structure of the budding yeast telom...

A modular strategy combining genetic code expansion with a selective amide bond-forming reaction enables the site-specific installation of lysine acylations on folded proteins. Acylboronates react sel...

Protein-protein interactions (PPIs) drive virtually all biological processes, yet most PPIs have not been identified and even more remain structurally unresolved. We developed a two-step computational...

Genome replication in eukaryotic cells is surveyed by the S-phase checkpoint, which orchestrates sequential replication origin activation to avoid exhaustion of hitherto poorly defined rate-limiting r...

Losing parental histones during DNA replication fork passage challenges differentiation competence and cell viability.

Cells activate various pathways to counter DNA replication stress. Using Replication-IDentifier (Repli-ID), the authors identified 423 genes, including LGE1 and ROX1, linking epigenetic and metabolic ...