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Exceptional brain and ecological diversity in the earliest snakes
Micro-CT scanning and digital reconstruction of T. mirim The holotype MPM 420 of T. mirim was scanned in the Laboratório de Caracterização Tecnológica of the Escola Politécnica at the University of São Paulo using a Zeiss Xradia Versa XRM-510 Scanner. The specimen was scanned in three parts: one for the skull and anteriormost cervicals, the second one focusing on posterior cervical vertebrae, and the third one on dorsal vertebrae. Parameters were as follows for all scans: 110 kV, 10 W, 25-s exposure (skull and cervicals) or 8 s (dorsals), and a 0.4× objective with HE6 filter resulting in 1,301 images. Voxel sizes were as follows: skull = 17.19 µm, cervicals = 16.2 µm and dorsals = 34 µm. Reconstruction was made with the software Xradia. Reconstruction and the parameters included a beam hardening of 0.08 and a smooth filter with a 0.5 kernel size. Segmentations of osteological structures, cranial endocasts and inner ear were performed using VGSTUDIO MAX v3.5., and 3D cinematic renderings were produced using the SmARTR pipeline36. Morphological and molecular phylogenetic data Here we used the last iteration41 of the total evidence dataset originally published by Simões et al.5, to assess the placement of Tametara not only among...