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NanoTag Biotechnologies

@nanotag.bsky.social
287 followers 784 following 112 posts

𝐂𝐮𝐬𝐭𝐨𝐦 𝐒𝐢𝐧𝐠𝐥𝐞-𝐃𝐨𝐦𝐚𝐢𝐧 𝐀𝐧𝐭𝐢𝐛𝐨𝐝𝐲 𝐃𝐢𝐬𝐜𝐨𝐯𝐞𝐫𝐲 & 𝐍𝐚𝐧𝐨𝐛𝐨𝐝𝐲-𝐁𝐚𝐬𝐞𝐝 𝐑𝐞𝐬𝐞𝐚𝐫𝐜𝐡 𝐓𝐨𝐨𝐥𝐬

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NanoTag Biotechnologies @nanotag.bsky.social · 06/10/2026
How do you enjoy the sunny days of autumn?☀️🍂 If you’re an alpaca, the answer is simple: find a patch of sand and roll in it. 🦙💨 Just like our alpacas enjoy a refreshing dust bath, our clients enjoy smooth single-domain antibody #sdAb discovery. Let’s roll into new discoveries together 🙃! #VHH #CRO
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NanoTag Biotechnologies @nanotag.bsky.social · 28/09/2026
✨🔬 Want to see our JF635b-conjugated #nanobodies in action? 📄 Check out the recent NanoLetters paper by Samrat Basak @samratb.bsky.social, Roman Tsukanov @romantsukanov.bsky.social, Felipe Opazo @opazo.bsky.social et al., doi.org/10.1021/acs....
doi.org
Self-Blinking Dye Restores Efficient Use of Nanobodies in Single-Molecule Localization Microscopy
Abstract. Direct stochastic optical reconstruction microscopy (dSTORM) relies on controlled fluorophore blinking to achieve nanometer-scale resolution, yet
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NanoTag Biotechnologies @nanotag.bsky.social · 28/09/2026
Spontaneous blinking. OneStep IF. Precise #SMLM. 🙌 Our Smart Secondaries® JF635b combine a self-blinking dye with small secondary #nanobodies for reduced linkage error, no clustering & fast OneStep IF. For #dSTORM & #MINFLUX 🔬✨ 🦙 Browse our JF635b-conjugated nanobodies here: bit.ly/4ffIkdB
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NanoTag Biotechnologies @nanotag.bsky.social · 22/09/2026
💬 “The ALFA-tag has been a game changer…” Great to hear this from Diane Lidke @dianelidke.bsky.social at The University of New Mexico School of Medicine @unm.edu! Her team uses #ALFAtag and #NbALFA for single-particle tracking & #dSTORM / #DNAPAINT 🔬 📄 #ALFAsystem 700+ publications and counting! 💪
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NanoTag Biotechnologies @nanotag.bsky.social · 07/09/2026
Greetings from #Horizons2026 in our hometown Göttingen! 🦙🔬Our team is here showcasing our #nanobody -based technologies and services. Come by our booth to explore how #NanoTag might support your research! #SingleDomainAntibody #FluoTag #SmartSecondaries #ALFAsystem @horizonsmolbio.bsky.social
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NanoTag Biotechnologies @nanotag.bsky.social · 04/09/2026
🔬It’s fantastic to see our Smart Secondaries® Halo fusion contributing to such an elegant and modular solution for fluorescence lifetime #multiplexing. Congratulations to the whole team on this impressive work 👏! Check available Halo fusion #nanobodies in our shop or contact us for custom solutions.
tinyurl.com
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NanoTag Biotechnologies @nanotag.bsky.social · 02/09/2026
#NanoTag mindset: 𝗡𝗼 𝗦𝗹𝗼𝗴𝗮𝗻𝘀. 𝗝𝘂𝘀𝘁 𝗦𝗰𝗶𝗲𝗻𝗰𝗲. We started in academia, developing #nanobodies for our own research - and we still approach our reagents as scientists: develop in-house, test thoroughly, characterise and share the data 📄. Because only reliable tools move #science forward 🚀
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Reposted by NanoTag Biotechnologies
Roman Tsukanov @romantsukanov.bsky.social · 27/08/2026
New publication alert 🚨 Our Nano Letters paper on self-blinking dyes with nanobodies for robust SMLM is now Open Access! pubs.acs.org/nalefd/artic... Commercial Nb–JF635b probes are available from @nanotag.bsky.social. Joint effort of amazing collaborators, inspired by the great @opazo.bsky.social
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NanoTag Biotechnologies @nanotag.bsky.social · 27/08/2026
🔬📄 You can precomplex the sdAb anti-Syt1 Luminal Domain ALFA-tag fusion with fluorescent NbALFA (FluoTag®-X2 anti-ALFA) or with gold-conjugated NbALFA. The latter approach was demonstrated in a recent paper by @forna.bsky.social et al. link.springer.com/article/10.1...
link.springer.com
A versatile nanobody platform for live and super-resolution imaging of synaptic vesicle dynamics and plasticity in rodent and human neurons - Journal of Nanobiotechnology
Synaptic neurotransmission is a critical hallmark of brain activity and one of the first processes affected in neural diseases. Monitoring this process, particularly synaptic vesicle recycling, in liv...
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NanoTag Biotechnologies @nanotag.bsky.social · 27/08/2026
🔬🫧🧠 NEW Product Alert: Targeting luminal #Syt1 with flexible readouts powered by the #ALFAsystem! Meet our new sdAb anti-Syt1 Luminal Domain ALFA-tag fusion (N4843) – combine it with different #NbALFA -based reagents for fluorescence imaging or EM. 🔗 Explore more: nano-tag.com/product/sdab...
Inside synaptic vesicle lumen sdAb anti-Syt1 Luminal Domain ALFA-tag fusion (N4843, NanoTag Biotechnologies) binds to the luminal domain of Syt1. The ALFA-tag is detected by NbALFA coupled to application-specific labels, e.g. fluorophores or nanogold particles.
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NanoTag Biotechnologies @nanotag.bsky.social · 14/08/2026
📸 Fig. 3: Two-colour me4Pi-SMLM imaging. 📄 Read the paper here: doi.org/10.1038/s415...
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NanoTag Biotechnologies @nanotag.bsky.social · 14/08/2026
🔬 Exciting to see our sdAb tools in new #microscopy approaches! In recent Nature Biotechnology @yongdengzhang.bsky.social & colleagues introduce me4Pi-SMLM, achieving near-isotropic 3D localization precision of 2–3 nm. Happy to see our anti-ALFA, anti-GFP & anti-RFP sdAbs used in this work! #SMLM
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NanoTag Biotechnologies @nanotag.bsky.social · 17/07/2026
📄 Read the comprehensive characterization of NbLumSyt1 (= sdAb anti-Syt1 Luminal Domain) toolset, recently published by Goel, Fornasiero @forna.bsky.social et al. in the Journal of Nanobiotechnology @springernature.com: link.springer.com/article/10.1...
link.springer.com
A versatile nanobody platform for live and super-resolution imaging of synaptic vesicle dynamics and plasticity in rodent and human neurons - Journal of Nanobiotechnology
Synaptic neurotransmission is a critical hallmark of brain activity and one of the first processes affected in neural diseases. Monitoring this process, particularly synaptic vesicle recycling, in liv...
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NanoTag Biotechnologies @nanotag.bsky.social · 17/07/2026
🔗 Learn more about the 𝘀𝗱𝗔𝗯 𝗮𝗻𝘁𝗶-𝗦𝘆𝘁𝟭 𝗟𝘂𝗺𝗶𝗻𝗮𝗹 𝗗𝗼𝗺𝗮𝗶𝗻 𝗽𝗛𝗹𝘂𝗼𝗿𝗶𝗻 𝗳𝘂𝘀𝗶𝗼𝗻 reagent and explore the live-cell labeling protocol here: nano-tag.com/product/sdab...
nano-tag.com
sdAb anti-Syt1 Luminal Domain pHluorin fusion - NanoTag Biotechnologies
pHluorin-fused sdAb targeting the luminal/extracellular domain of Synaptotagmin 1 (Syt1) for pH-sensitive live-cell imaging of active synapses.
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NanoTag Biotechnologies @nanotag.bsky.social · 17/07/2026
🔬🫧💚 #FluorescenceFriday New Product – sdAb anti-Syt1 Luminal Domain #pHluorin fusion! A pH-sensitive reporter combining our sdAb anti-Syt1 Luminal Domain with superecliptic pHluorin for real-time monitoring of #SynapticVesicles exo-endocytic cycling. ♻️ #Neuroscience #Nanobody #Synaptotagmin1
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NanoTag Biotechnologies @nanotag.bsky.social · 08/07/2026
👋 ¡Hola from Barcelona! 🇪🇸☀️ Great start to #FENS2026! Visit NanoTag at Booth #43 to explore our #nanobody tools for #Immunofluorescence, #OneStepIF, #Multiplexing, #SuperResolution & #Pulldowns. Our team looks forward to meeting you!🦙🧠✨ #SingleDomainAntibodies #Neuroscience
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Reposted by NanoTag Biotechnologies
Joachim Goedhart @joachimgoedhart.bsky.social · 01/07/2026
A High-Affinity Nanobody Recognizing mNeonGreen Enables Versatile Biochemical, Cellular, and in vivo Applications. by Steffen Frey and team: www.biorxiv.org/content/10.6...
mNeonGreen Selector: a pulldown resin for mNeonGreen-fusion proteins. (A) Sketch of mNG
Selector binding mNG fusion proteins. (B, C) Immunoprecipitation from E.coli lysate in the presence (B) or
absence (C) of target protein (mNG-Halo fusion, arrowhead). Proteins were eluted in boiling SDS sample
buffer. mNG Selector enables highly specific one-step capture of mNG-tagged proteins. (D) Binding assay to
evaluate the cross-reactivity of the mNG Selector to other recombinant fluorescent proteins. Shown is a figure
of the equilibrium bound fraction of each indicated fluorescent protein prior to buffer washing. (E)
Compatibility of mNG Selector to various reagents applied during binding (top panel) or during washing steps
(lower panel). mNG fluorescence is lost in the presence of 2 M Gua-HCl. The symbols below each row indicate
binding efficiency: ++: strong binding, + binding, -: no binding
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Reposted by NanoTag Biotechnologies
Felipe Opazo @opazo.bsky.social · 02/07/2026
New paper! 🚀 A high-affinity anti- #mNeonGreen #nanobody turns #mNG into a #multifunctional Tag. @nanotag.bsky.social decided to openly share the #nanobody for research use. Not common, but we strongly believe well-defined reagents are key for reproducible science. We hope you find it useful! 💚
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NanoTag Biotechnologies @nanotag.bsky.social · 03/07/2026
Proud of our team and grateful to everyone who contributed to this journey. This paper reflects our shared passion for science and our commitment to developing reliable research tools for the scientific community. 🦙💚🔬
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NanoTag Biotechnologies @nanotag.bsky.social · 02/07/2026
🇪🇸Heading to #FENS2026 in Barcelona? Visit #NanoTag at Booth #43! Discover our in-house developed #nanobody reagents for #imaging, protein analysis, and #neuroscience – from FluoTags, Smart Secondaries, rHcAbs to the ALFA System and Selector Resins 🦙🧠🔬. 💬 Stop by for a chat!
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NanoTag Biotechnologies @nanotag.bsky.social · 30/06/2026
Heading to #FEBS2026? Stop by Booth #7! #NanoTag team will be there to discuss how our #nanobody technologies can support your research🦙🔬🧪 Explore the ALFA system, FluoTags, Smart Secondaries, rHcAbs & Selector Resins. See you in Maastricht! 👋 #sdAb #Immunostaining #Microscopy #ProteinAnalysis
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NanoTag Biotechnologies @nanotag.bsky.social · 22/06/2026
Congratulations @syncellbiolab.bsky.social team on this impressive work! 👏 It's exciting to see IntAct-U-ExM revealing actin architectures with such remarkable detail. Happy to see our #ALFAsystem enabling robust post-expansion labeling & high-resolution visualization of complex actin networks. 🦙🔬
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NanoTag Biotechnologies @nanotag.bsky.social · 22/06/2026
Did you know? #NanoTag provides #CRO services for custom single-domain antibody (sdAb) discovery & protein production🦙🧬🧑‍🔬 Meet our CEO at #BIO2026 in San Diego 🇺🇸 this week at the German Pavilion or get in touch via our website to discuss your project: nano-tag.com/contact/ #VHH #ProteinProduction
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Reposted by NanoTag Biotechnologies
abberior @abberior.rocks · 15/06/2026
Multiplexing with same-species primary antibodies, solved with Premix&Stain & Smart Secondary Nanobodies. Actin · GFP tag · Golgi · NUP · Mitochondria. New: STAR deepRED & STAR 550 expand the FluoTag® portfolio. @nanotag.bsky.social #SmartSecondaries #MulticolorImaging #abberior
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NanoTag Biotechnologies @nanotag.bsky.social · 15/06/2026
Explore FluoTags® with the expanded abberior STAR dye range here: tinyurl.com/3zd4bxbv
tinyurl.com
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NanoTag Biotechnologies @nanotag.bsky.social · 15/06/2026
📸 3plex IF using two mouse primary Abs premixed with #SmartSecondaries for 15 min at RT & combined with FluoTag®-X4 anti-GFP STAR deepRED for OneStep staining: 🟠 Vimentin – FluoTag®-X2 anti-Mouse IgG1 STAR ORANGE 🔵 NPC – FluoTag®-X2 anti-Mouse IgG1 STAR RED 🔴 NUP96-GFP – FluoTag®-X4 anti-GFP deepRED
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NanoTag Biotechnologies @nanotag.bsky.social · 15/06/2026
🔬🌈 We're expanding the spectral range of #abberior STAR-conjugated #nanobodies! 🤩 STAR 550 and deepRED have joined the range. Choose Smart Secondaries® and FluoTag®-X4 anti-GFP in your favorite colors and #multiplex with ease. 🦙🍩 In collaboration with @abberior.rocks team! #Microscopy #STED
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NanoTag Biotechnologies @nanotag.bsky.social · 11/06/2026
#NanoTag is exhibiting at #BIO2026 in San Diego!🇺🇸 Meet our CEO Dr. Hansjörg Götzke at German Pavilion, booth 3335-AF, to explore how our 🦙 sdAb discovery platforms & protein engineering services advance life science and biomedical research. #SingleDomainAntibodies #ProteinProduction #CROservices
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NanoTag Biotechnologies @nanotag.bsky.social · 08/06/2026
🔬🦙 Explore the reagents and live-cell labeling protocol here: tinyurl.com/2yxu5hma
tinyurl.com
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NanoTag Biotechnologies @nanotag.bsky.social · 08/06/2026
📄 Read the comprehensive characterization of NbLumSyt1 (= sdAb anti-Syt1 Luminal Domain) toolset, recently published by Goel R, Fornasiero EF @forna.bsky.social et al. in the Journal of Nanobiotechnology: link.springer.com/article/10.1...
link.springer.com
A versatile nanobody platform for live and super-resolution imaging of synaptic vesicle dynamics and plasticity in rodent and human neurons - Journal of Nanobiotechnology
Synaptic neurotransmission is a critical hallmark of brain activity and one of the first processes affected in neural diseases. Monitoring this process, particularly synaptic vesicle recycling, in liv...
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NanoTag Biotechnologies @nanotag.bsky.social · 08/06/2026
🔬🫧 New tools for live-cell monitoring of #SynapticVesicle dynamics based on a #nanobody targeting the Syt1 Luminal Domain are now available in the NanoTag Shop: • FluoTag®-X2 anti-Syt1 Luminal Domain • sdAb anti-Syt1 Luminal Domain Halo fusion 🔥More anti-Syt1 Luminal Domain reagents coming soon!
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NanoTag Biotechnologies @nanotag.bsky.social · 22/05/2026
#Neurizons2026 is wrapping up today! Thanks to everyone who stopped by the #NanoTag booth to chat about single-domain antibody tools. 🔬 Big thanks to the organizers and all participants for making this event such a great opportunity to connect, share ideas, and talk #neuroscience. 🧠
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NanoTag Biotechnologies @nanotag.bsky.social · 15/05/2026
NanoTag will be at #Neurizons2026! Visit our booth to explore #nanobody-based tools for super-resolution microscopy and protein analysis 🦙🧠🔬. 👋See you at Max Planck Institute for Multidisciplinary Sciences @mpi-nat.bsky.social #Neuroscience #SingleDomainAntibody
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NanoTag Biotechnologies @nanotag.bsky.social · 11/05/2026
💡Did you know? The Super-Resolution Specialist School 2026 by @esric-imaging.bsky.social is happening this week in Edinburgh 🔬 #NanoTag is happy to contribute! Our CTO Felipe Opazo @opazo.bsky.social will give a talk on #nanobody tools & #multiplexing strategies for advanced #imaging.
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NanoTag Biotechnologies @nanotag.bsky.social · 06/05/2026
Interested in uncovering nervous system details at super-resolution? 🔬 Take a look at NanoTag’s nanobody-based tools for neurobiological imaging 👉 bit.ly/4utsjIW
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NanoTag Biotechnologies @nanotag.bsky.social · 06/05/2026
Astrocytes never fail to amaze 🫠✨ This beautiful GFAP⁺ network by Svilen Georgiev (Rizzoli Lab, UMG) was stained with our fluorescent #nanobody FluoTag®-X2 anti-GFAP, revealing an intricate, highly interconnected network of filament-rich processes 🧠 #sdAb #Microscopy #Neuroscience #Astrocytes #GFAP
Primary rat brain culture showing GFAP-positive astrocytes, stained with the fluorescent nanobody FluoTag®-X2 anti-GFAP (NanoTag #N3802). Image courtesy of Svilen Georgiev.
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NanoTag Biotechnologies @nanotag.bsky.social · 28/04/2026
Curious about #SmartSecondaries® – our secondary nanobodies for same-species multiplexing & OneStep IF 🦙? Learn more 👉 nano-tag.com/technology/s...
nano-tag.com
Smart Secondaries® - NanoTag Biotechnologies
Ideal secondary Ab replacement for One Step IF, higher resolution & species-independent multiplexing. Discover benefits of Smart Secondaries®!
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NanoTag Biotechnologies @nanotag.bsky.social · 28/04/2026
At a recent conference, Nahima Saliba @gustavssonlab.bsky.social, Rice University stopped by the #NanoTag booth and shared how much she enjoys using our secondary sdAbs #SmartSecondaries® for DNA-PAINT 🔬🎨 Faster, easier #multiplexing with a pre-mixing protocol – exactly what they’re designed for 💙
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NanoTag Biotechnologies @nanotag.bsky.social · 17/04/2026
Never worked with #SmartSecondaries® (secondary nanobodies) for detecting conventional or recombinant primary antibodies? Here you can find more information: nano-tag.com/technology/s...
nano-tag.com
Smart Secondaries® - NanoTag Biotechnologies
Ideal secondary Ab replacement for One Step IF, higher resolution & species-independent multiplexing. Discover benefits of Smart Secondaries®!
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NanoTag Biotechnologies @nanotag.bsky.social · 17/04/2026
📸 Left panel: PFA-fixed rat brain stained with rHcAb anti-CNPase Rb IgG Fc-fusion, detected with Smart Secondary® – FluoTag®-X2 anti-Rb IgG. Right panel: PFA-fixed rat primary neurons stained with rHcAb anti-SYT1 (7D5) Ms IgG1 Fc-fusion, detected with Smart Secondary® – FluoTag®-X2 anti-Ms IgG1.
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NanoTag Biotechnologies @nanotag.bsky.social · 17/04/2026
📣 Neuroscientists – something NEW for you! NanoTag expands its portfolio of neurobiological #rHcAbs 🧠. Nanobodies targeting #SYT1 and #CNPase are now available as Mouse or Rabbit IgG Fc fusions – ideal for ICC and IHC 🔬. Learn more 👉 bit.ly/4cKITOd #Neuroscience #RecombinantAntibody
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NanoTag Biotechnologies @nanotag.bsky.social · 10/04/2026
📄 A recent study in #ACSNano by @heilemannlab.bsky.social shows that 🔬STED microscopy with DNA-fluorophore labels enables intensity-based molecular counting. Happy to see our #nanobodies 🦙 FluoTag®-X4 anti-GFP and sdAb anti-ALFA contribute to this work. Read the paper here: doi.org/10.1021/acsn...
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NanoTag Biotechnologies @nanotag.bsky.social · 01/04/2026
🌸 Springtime – where flowers and ideas bloom! Whether you’re chasing big questions or exploring tiny molecules, our #nanobody tools & custom #sdAb services help you make egg-cellent progress. 🐣🔬🦙 Wishing you flourishing research and plenty of hoppy moments 🐇! #HappyEaster from #NanoTag!
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NanoTag Biotechnologies @nanotag.bsky.social · 30/03/2026
Meet #NanoTag at the #FOM2026 in Stockholm! Visit booth 24 to explore our 🦙 #nanobody -based solutions tailored for quantitative super-resolution microscopy and flexible #multiplexing 🔬. Our team is looking forward to connecting with you! #SingleDomainAntibodies #SmartSecondaries #FluoTag
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NanoTag Biotechnologies @nanotag.bsky.social · 18/03/2026
Join #NanoTag team at #FOM2026 in Stockholm 🇸🇪! Visit booth #24 to see how our single-domain antibody (sdAb) technologies bring greater precision to #microscopy and more flexibility & ease to #multiplexing 🔬 See you in Stockholm! 👋 #SuperResolution #Nanoscopy #Nanobody #Immunofluorescence
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NanoTag Biotechnologies @nanotag.bsky.social · 10/03/2026
📄🦠 New publication! In collaboration with #Takeda our synthetic single-domain antibody library enabled rapid discovery of strain-specific sdAbs against SARS-CoV-2 variants, supporting vaccine identification testing. Read more in Antibodies @mdpiopenaccess.bsky.social 👉 doi.org/10.3390/anti...
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NanoTag Biotechnologies @nanotag.bsky.social · 04/03/2026
For flexible HaloTag-based imaging, explore our Halo fusion nanobody portfolio or contact us for custom solutions 👉 bit.ly/3OEXiSK
bit.ly
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NanoTag Biotechnologies @nanotag.bsky.social · 04/03/2026
📄🔬 Congratulations to all authors on this excellent preprint showcasing #NanoFLex, a FLIM strategy that enables lifetime-based separation of multiple targets in a OneStep-IF using our #SmartSecondaries fused to different #HaloTag variants. 👏 We’re proud that SmartSecondaries® power this workflow☺️
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NanoTag Biotechnologies @nanotag.bsky.social · 24/02/2026
We’re having a fantastic time at #BPS2026 in San Francisco 🌁 Last chance to stop by booth 720 to meet the #NanoTag team and learn how our nanobody-based reagents 🔬 and sdAb services 🦙 can advance your research. Thanks for the great discussions and the positive feedback on our #sdAb technologies💙!
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NanoTag Biotechnologies @nanotag.bsky.social · 19/02/2026
Special thanks to 𝐒𝐚𝐛𝐫𝐢𝐧𝐚 𝐏𝐮𝐭𝐢𝐠𝐧𝐚𝐧𝐨 (FU Berlin @freieuniversitaet.bsky.social) for sharing these D. melanogaster multiplexing images with us 💙
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