Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 06/10/2026How do you enjoy the sunny days of autumn?☀️🍂 If you’re an alpaca, the answer is simple: find a patch of sand and roll in it. 🦙💨 Just like our alpacas enjoy a refreshing dust bath, our clients enjoy smooth single-domain antibody #sdAb discovery. Let’s roll into new discoveries together 🙃! #VHH #CRO 021
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 28/09/2026✨🔬 Want to see our JF635b-conjugated #nanobodies in action? 📄 Check out the recent NanoLetters paper by Samrat Basak @samratb.bsky.social, Roman Tsukanov @romantsukanov.bsky.social, Felipe Opazo @opazo.bsky.social et al., doi.org/10.1021/acs....doi.orgSelf-Blinking Dye Restores Efficient Use of Nanobodies in Single-Molecule Localization MicroscopyAbstract. Direct stochastic optical reconstruction microscopy (dSTORM) relies on controlled fluorophore blinking to achieve nanometer-scale resolution, yet 064
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 28/09/2026Spontaneous blinking. OneStep IF. Precise #SMLM. 🙌 Our Smart Secondaries® JF635b combine a self-blinking dye with small secondary #nanobodies for reduced linkage error, no clustering & fast OneStep IF. For #dSTORM & #MINFLUX 🔬✨ 🦙 Browse our JF635b-conjugated nanobodies here: bit.ly/4ffIkdB 122
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 22/09/2026💬 “The ALFA-tag has been a game changer…” Great to hear this from Diane Lidke @dianelidke.bsky.social at The University of New Mexico School of Medicine @unm.edu! Her team uses #ALFAtag and #NbALFA for single-particle tracking & #dSTORM / #DNAPAINT 🔬 📄 #ALFAsystem 700+ publications and counting! 💪 031
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 07/09/2026Greetings from #Horizons2026 in our hometown Göttingen! 🦙🔬Our team is here showcasing our #nanobody -based technologies and services. Come by our booth to explore how #NanoTag might support your research! #SingleDomainAntibody #FluoTag #SmartSecondaries #ALFAsystem @horizonsmolbio.bsky.social 011
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 04/09/2026🔬It’s fantastic to see our Smart Secondaries® Halo fusion contributing to such an elegant and modular solution for fluorescence lifetime #multiplexing. Congratulations to the whole team on this impressive work 👏! Check available Halo fusion #nanobodies in our shop or contact us for custom solutions.tinyurl.com 041
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 02/09/2026#NanoTag mindset: 𝗡𝗼 𝗦𝗹𝗼𝗴𝗮𝗻𝘀. 𝗝𝘂𝘀𝘁 𝗦𝗰𝗶𝗲𝗻𝗰𝗲. We started in academia, developing #nanobodies for our own research - and we still approach our reagents as scientists: develop in-house, test thoroughly, characterise and share the data 📄. Because only reliable tools move #science forward 🚀 062
Reposted by Karina Kaczmarek-Hajek, PhDRoman Tsukanov @romantsukanov.bsky.social · 27/08/2026New publication alert 🚨 Our Nano Letters paper on self-blinking dyes with nanobodies for robust SMLM is now Open Access! pubs.acs.org/nalefd/artic... Commercial Nb–JF635b probes are available from @nanotag.bsky.social. Joint effort of amazing collaborators, inspired by the great @opazo.bsky.social 0124
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 27/08/2026🔬🫧🧠 NEW Product Alert: Targeting luminal #Syt1 with flexible readouts powered by the #ALFAsystem! Meet our new sdAb anti-Syt1 Luminal Domain ALFA-tag fusion (N4843) – combine it with different #NbALFA -based reagents for fluorescence imaging or EM. 🔗 Explore more: nano-tag.com/product/sdab... 121
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 14/08/2026🔬 Exciting to see our sdAb tools in new #microscopy approaches! In recent Nature Biotechnology @yongdengzhang.bsky.social & colleagues introduce me4Pi-SMLM, achieving near-isotropic 3D localization precision of 2–3 nm. Happy to see our anti-ALFA, anti-GFP & anti-RFP sdAbs used in this work! #SMLM 121
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 17/07/2026🔬🫧💚 #FluorescenceFriday New Product – sdAb anti-Syt1 Luminal Domain #pHluorin fusion! A pH-sensitive reporter combining our sdAb anti-Syt1 Luminal Domain with superecliptic pHluorin for real-time monitoring of #SynapticVesicles exo-endocytic cycling. ♻️ #Neuroscience #Nanobody #Synaptotagmin1 121
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 08/07/2026👋 ¡Hola from Barcelona! 🇪🇸☀️ Great start to #FENS2026! Visit NanoTag at Booth #43 to explore our #nanobody tools for #Immunofluorescence, #OneStepIF, #Multiplexing, #SuperResolution & #Pulldowns. Our team looks forward to meeting you!🦙🧠✨ #SingleDomainAntibodies #Neuroscience 011
Reposted by Karina Kaczmarek-Hajek, PhDJoachim Goedhart @joachimgoedhart.bsky.social · 01/07/2026A High-Affinity Nanobody Recognizing mNeonGreen Enables Versatile Biochemical, Cellular, and in vivo Applications. by Steffen Frey and team: www.biorxiv.org/content/10.6... 27333
Reposted by Karina Kaczmarek-Hajek, PhDFelipe Opazo @opazo.bsky.social · 02/07/2026New paper! 🚀 A high-affinity anti- #mNeonGreen #nanobody turns #mNG into a #multifunctional Tag. @nanotag.bsky.social decided to openly share the #nanobody for research use. Not common, but we strongly believe well-defined reagents are key for reproducible science. We hope you find it useful! 💚 610051
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 02/07/2026🇪🇸Heading to #FENS2026 in Barcelona? Visit #NanoTag at Booth #43! Discover our in-house developed #nanobody reagents for #imaging, protein analysis, and #neuroscience – from FluoTags, Smart Secondaries, rHcAbs to the ALFA System and Selector Resins 🦙🧠🔬. 💬 Stop by for a chat! 011
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 30/06/2026Heading to #FEBS2026? Stop by Booth #7! #NanoTag team will be there to discuss how our #nanobody technologies can support your research🦙🔬🧪 Explore the ALFA system, FluoTags, Smart Secondaries, rHcAbs & Selector Resins. See you in Maastricht! 👋 #sdAb #Immunostaining #Microscopy #ProteinAnalysis 011
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 22/06/2026Congratulations @syncellbiolab.bsky.social team on this impressive work! 👏 It's exciting to see IntAct-U-ExM revealing actin architectures with such remarkable detail. Happy to see our #ALFAsystem enabling robust post-expansion labeling & high-resolution visualization of complex actin networks. 🦙🔬 021
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 22/06/2026Did you know? #NanoTag provides #CRO services for custom single-domain antibody (sdAb) discovery & protein production🦙🧬🧑🔬 Meet our CEO at #BIO2026 in San Diego 🇺🇸 this week at the German Pavilion or get in touch via our website to discuss your project: nano-tag.com/contact/ #VHH #ProteinProduction 021
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 15/06/2026🔬🌈 We're expanding the spectral range of #abberior STAR-conjugated #nanobodies! 🤩 STAR 550 and deepRED have joined the range. Choose Smart Secondaries® and FluoTag®-X4 anti-GFP in your favorite colors and #multiplex with ease. 🦙🍩 In collaboration with @abberior.rocks team! #Microscopy #STED 131
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 11/06/2026#NanoTag is exhibiting at #BIO2026 in San Diego!🇺🇸 Meet our CEO Dr. Hansjörg Götzke at German Pavilion, booth 3335-AF, to explore how our 🦙 sdAb discovery platforms & protein engineering services advance life science and biomedical research. #SingleDomainAntibodies #ProteinProduction #CROservices 031
Reposted by Karina Kaczmarek-Hajek, PhDabberior @abberior.rocks · 05/06/2026abberior STAR deepRED is now available as a nanobody conjugate ⭐ More spectral options, more flexibility for multicolor STED. Developed in collaboration with NanoTag Biotechnologies – now available for anti-GFP, anti-mouse and anti-rabbit Smart Secondaries®. #abberior #NanoTagBiotechnologies #STED 221
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 22/05/2026#Neurizons2026 is wrapping up today! Thanks to everyone who stopped by the #NanoTag booth to chat about single-domain antibody tools. 🔬 Big thanks to the organizers and all participants for making this event such a great opportunity to connect, share ideas, and talk #neuroscience. 🧠 011
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 15/05/2026NanoTag will be at #Neurizons2026! Visit our booth to explore #nanobody-based tools for super-resolution microscopy and protein analysis 🦙🧠🔬. 👋See you at Max Planck Institute for Multidisciplinary Sciences @mpi-nat.bsky.social #Neuroscience #SingleDomainAntibody 021
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 06/05/2026Astrocytes never fail to amaze 🫠✨ This beautiful GFAP⁺ network by Svilen Georgiev (Rizzoli Lab, UMG) was stained with our fluorescent #nanobody FluoTag®-X2 anti-GFAP, revealing an intricate, highly interconnected network of filament-rich processes 🧠 #sdAb #Microscopy #Neuroscience #Astrocytes #GFAP 131
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 28/04/2026At a recent conference, Nahima Saliba @gustavssonlab.bsky.social, Rice University stopped by the #NanoTag booth and shared how much she enjoys using our secondary sdAbs #SmartSecondaries® for DNA-PAINT 🔬🎨 Faster, easier #multiplexing with a pre-mixing protocol – exactly what they’re designed for 💙 131
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 17/04/2026📣 Neuroscientists – something NEW for you! NanoTag expands its portfolio of neurobiological #rHcAbs 🧠. Nanobodies targeting #SYT1 and #CNPase are now available as Mouse or Rabbit IgG Fc fusions – ideal for ICC and IHC 🔬. Learn more 👉 bit.ly/4cKITOd #Neuroscience #RecombinantAntibody 132
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 10/04/2026📄 A recent study in #ACSNano by @heilemannlab.bsky.social shows that 🔬STED microscopy with DNA-fluorophore labels enables intensity-based molecular counting. Happy to see our #nanobodies 🦙 FluoTag®-X4 anti-GFP and sdAb anti-ALFA contribute to this work. Read the paper here: doi.org/10.1021/acsn... 062
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 18/03/2026Join #NanoTag team at #FOM2026 in Stockholm 🇸🇪! Visit booth #24 to see how our single-domain antibody (sdAb) technologies bring greater precision to #microscopy and more flexibility & ease to #multiplexing 🔬 See you in Stockholm! 👋 #SuperResolution #Nanoscopy #Nanobody #Immunofluorescence 131
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 10/03/2026📄🦠 New publication! In collaboration with #Takeda our synthetic single-domain antibody library enabled rapid discovery of strain-specific sdAbs against SARS-CoV-2 variants, supporting vaccine identification testing. Read more in Antibodies @mdpiopenaccess.bsky.social 👉 doi.org/10.3390/anti... 011
Reposted by Karina Kaczmarek-Hajek, PhDFelipe Opazo @opazo.bsky.social · 04/03/2026New preprint out! In this study, we introduce NanoFLex, a strategy that combines #HaloTag variants with #nanobodies -based immunolabeling to enable rapid, OneStep-IF #lifetime #multiplexing. Possible due to SmartSecondaries fused to #HaloTag from @nanotag.bsky.social: www.biorxiv.org/content/10.6... 17437
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 24/02/2026We’re having a fantastic time at #BPS2026 in San Francisco 🌁 Last chance to stop by booth 720 to meet the #NanoTag team and learn how our nanobody-based reagents 🔬 and sdAb services 🦙 can advance your research. Thanks for the great discussions and the positive feedback on our #sdAb technologies💙! 021
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 19/02/2026Did you know #multiplex IF can be done in just one incubation step? ❌ No sequential staining ❌ No host-species limits ❌ No secondary-induced clustering 🔬Pre-mix your favourite primary antibodies (even same-species) with Smart Secondaries® #nanobody & apply directly to your sample for OneStep IF.🤩 141
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 13/02/2026Neuroscientists - looking for a high-affinity gift?💝 Inside the postsynapse, proteins crowd the show PSD95’s the star - but hard to know. IgG antibodies knock, but can’t get through Too bulky to reach the target they pursue. But FluoTag®-X2 anti-PSD95 slips right in Binds PSD95. Precise within. 111
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 12/02/2026Heading to #BPS2026 in San Francisco? Visit NanoTag team at booth 720 to explore our single-domain antibody #sdAb tools: ALFA® System, FluoTags®, Smart Secondaries® & Selector Resins. See you! 👋 @biophysicalsoc.bsky.social #Nanobody #Multiplexing #SuperResolution #Microscopy #AffinityPurification 011
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 03/02/2026💬 Thank you, Bhanu Prakash Potlapalli, for the great feedback on the ALFA® system! 😊 Applied in a CRISPR–dCas9 DNA imaging approach for efficient labeling of repetitive DNA sequences and live-cell imaging of plant telomeres doi.org/10.1093/jxb/... Explore #ALFAsystem 👉 nano-tag.com/technology/t... 041
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 28/01/2026Did you know? #NanoTag offers custom single-domain antibody (sdAb) discovery services, including alpaca immunization🦙and synthetic library–based approaches🧬backed by over a decade of experience in the field 💪. Discuss your project with our experts: nano-tag.com/contact/ #CRO #Nanobody #VHH #sdAb 011
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 21/01/2026🔬 Join Imaging Bites seminar 28 Jan at 12:30 at @kingscollegelondon.bsky.social. Prof. Dr. Felipe Opazo @opazo.bsky.social presents #OneStep-IF, a simplified approach to highly #multiplexed IF using same-species primaries and Smart Secondaries® premixes. Register here: www.kcl.ac.uk/events/imagi... 063
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 11/12/2025#CellBio2025 in Philadelphia has come to a close! Thanks to everyone who stopped by the #NanoTag booth 🦙 – great conversations and insights all around. We were excited to hear how our sdAb tools are driving your new discoveries 📄👏 #SingleDomainAntibody #ALFAsystem #SmartSecondaries 051
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 06/12/2025What most people don’t see is the behind-the-scenes effort- not magic, just #teamwork. Karina & Steffen have #NanoTag booth ready for #CellBio2025 in Philadelphia. Starting Sunday, discover how our single-domain antibody solutions can support your research🔬🦙. See you ascbiology.bsky.social 👋 031
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 25/11/2025Attending #CellBio2025 in Philadelphia Dec 6–10? Visit #NanoTag team at booth #1117 to explore our #sdAb technologies & ready-to-use reagents: ALFA® System, FluoTags®, Smart Secondaries®, Selector Resins. See you there! 👋 @ascbiology.bsky.social #Nanobody #CellBiology #Immunofluorescence #PullDown 043
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 21/11/2025Join our CTO, Felipe Opazo @opazo.bsky.social, at #MBSJ2025 @mbsj-official.bsky.social as he presents new super-resolution #multiplexing advances powered by #SmartSecondaries®. 🔬Discover flexible, scalable and precise multi-target imaging. #Nanobody #NanoPlex #SUMPAINT #SuperResolution #Microscopy 031
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 06/11/2025Have you heard of #ANGEL? Not this 😇, but ALFA Nanobody-Guided Endogenous Labeling #ANGEL - the new approach published by Wang et al. in @natchembio.nature.com Build on our ALFA® System, it enables direct fluorescent readout of #CRISPR knock-ins. 📄Full paper here: bit.ly/47XgK48bit.lyALFA nanobody-guided endogenous labeling - Nature Chemical BiologySmall peptide tags (like ALFA) cause minimal disruption of proteins, but they typically lack fluorescence, which would enable knockin screening. Now, antigen-stabilizing fluorescent protein-fused nano... 122
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 30/09/2025🔬✨ #GEF25 Expansion Microscopy Forum in Göttingen just wrapped up! We were happy to support as sponsors and share our sdAb innovations for super-resolution #microscopy. Thanks to the organizers & all participants for an inspiring event! 👏 #ExM #SmartSecondaries #Multiplexing 021
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 17/09/2025#ExM turned 10 this year - just like #NanoTag! 🎉 Join us & the super-resolution community in Göttingen for the ExM Forum! 🔬Hear from our founder Felipe Opazo @opazo.bsky.social and CSO Steffen Frey on how NanoTag’s sdAb solutions empower nanoscopy & #multiplex imaging. See you in Göttingen! #GEF25 0116
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 04/09/2025📣 Expanding our Halo portfolio! Introducing Halo Selector - #sdAb -based affinity resin for highly specific, easy & reliable #HaloTag fusion protein #pulldown. ✅ High affinty & capacity ✅ Ultra- low background ✅ Broad buffer compatibility ✅ Works with bound ligands Learn more: bit.ly/465BKnm 031
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 14/08/2025🔬 Two weeks to go until the 14th #SMLM2025 @smlm-symposium.bsky.social! NanoTag joins as Gold Sponsor to support this unique #SuperResolution community event. Don't miss our CSO Dr. Steffen Frey talk on Smart Secondaries® (27 Aug, 14:40) or meet him at the #NanoTag booth 🦙. #Nanobody #Multiplexing 042
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 01/08/2025📄A new Nature Commun. paper by Rahman F. et al. maps nuclear organization in 3D at super-resolution using multiplexed Exchange-PAINT & HIST. 🔬 12 nuclear proteins were detected using our Smart Secondaries® #nanobody 🦙technology, customised by @massivephotonics.bsky.social 🎨. doi.org/10.1038/s414...doi.orgMapping the nuclear landscape with multiplexed super-resolution fluorescence microscopy - Nature CommunicationsThe cell nucleus coordinates diverse functions. Here, the authors utilise multiplexed super-resolution imaging to study how nuclear proteins are distributed relative to each other from the micro to th... 041
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 08/07/2025🎨 Precision and Molecular imaging need a broad palette. FluoTags® are fluorescent #sdAbs/ #nanobodies available in Alexa, Atto, AbberiorSTAR, JF635b, IRDyes & more for #Confocal, #STED, #STORM, and #NIR imaging. Custom dye? No ProbLama! nano-tag.com/shop/ #Microscopy #Nanobody #Multiplexing 051
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 28/05/2025🤹♀️ Still juggling primary antibodies from different species? Still stuck in sequential staining? ⏱️Switch to Smart Secondaries®🦙: ✅OneStep-IF ✅Same-species #multiplexing ✅Higher precision & reproducibility! Choose smart 👉 bit.ly/3UZxRMI #SecondaryNanobody #Microscopy #Immunofluorescence 043
Reposted by Karina Kaczmarek-Hajek, PhDNanoTag Biotechnologies @nanotag.bsky.social · 13/05/2025A new study by @jungmannlab.bsky.social presents #SPINNA, a model-based tool for quantifying protein stoichiometry, oligomerization state, and spatial arrangement from super-resolution data.🔬For precise labeling of #GFP & #mCherry fusion proteins researchers used our #nanobodies 😊 📄 bit.ly/4jTHfesbit.lySpatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution - Nature CommunicationsExtracting quantitative information on biomolecular oligomerisation with high resolution remains a significant challenge. Here, the authors propose SPINNA, a framework that compares nearest-neighbour ... 0154