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Karina Kaczmarek-Hajek, PhD

@karinakaczmarek.bsky.social
11 followers 90 following 0 posts

Life Scientist ǀ Passionately Curious & Creative ǀ Value Driven ǀ Customer-Centric Voice at NanoTag Biotechnologies

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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 06/10/2026
How do you enjoy the sunny days of autumn?☀️🍂 If you’re an alpaca, the answer is simple: find a patch of sand and roll in it. 🦙💨 Just like our alpacas enjoy a refreshing dust bath, our clients enjoy smooth single-domain antibody #sdAb discovery. Let’s roll into new discoveries together 🙃! #VHH #CRO
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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 28/09/2026
✨🔬 Want to see our JF635b-conjugated #nanobodies in action? 📄 Check out the recent NanoLetters paper by Samrat Basak @samratb.bsky.social, Roman Tsukanov @romantsukanov.bsky.social, Felipe Opazo @opazo.bsky.social et al., doi.org/10.1021/acs....
doi.org
Self-Blinking Dye Restores Efficient Use of Nanobodies in Single-Molecule Localization Microscopy
Abstract. Direct stochastic optical reconstruction microscopy (dSTORM) relies on controlled fluorophore blinking to achieve nanometer-scale resolution, yet
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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 28/09/2026
Spontaneous blinking. OneStep IF. Precise #SMLM. 🙌 Our Smart Secondaries® JF635b combine a self-blinking dye with small secondary #nanobodies for reduced linkage error, no clustering & fast OneStep IF. For #dSTORM & #MINFLUX 🔬✨ 🦙 Browse our JF635b-conjugated nanobodies here: bit.ly/4ffIkdB
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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 22/09/2026
💬 “The ALFA-tag has been a game changer…” Great to hear this from Diane Lidke @dianelidke.bsky.social at The University of New Mexico School of Medicine @unm.edu! Her team uses #ALFAtag and #NbALFA for single-particle tracking & #dSTORM / #DNAPAINT 🔬 📄 #ALFAsystem 700+ publications and counting! 💪
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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 07/09/2026
Greetings from #Horizons2026 in our hometown Göttingen! 🦙🔬Our team is here showcasing our #nanobody -based technologies and services. Come by our booth to explore how #NanoTag might support your research! #SingleDomainAntibody #FluoTag #SmartSecondaries #ALFAsystem @horizonsmolbio.bsky.social
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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 04/09/2026
🔬It’s fantastic to see our Smart Secondaries® Halo fusion contributing to such an elegant and modular solution for fluorescence lifetime #multiplexing. Congratulations to the whole team on this impressive work 👏! Check available Halo fusion #nanobodies in our shop or contact us for custom solutions.
tinyurl.com
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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 02/09/2026
#NanoTag mindset: 𝗡𝗼 𝗦𝗹𝗼𝗴𝗮𝗻𝘀. 𝗝𝘂𝘀𝘁 𝗦𝗰𝗶𝗲𝗻𝗰𝗲. We started in academia, developing #nanobodies for our own research - and we still approach our reagents as scientists: develop in-house, test thoroughly, characterise and share the data 📄. Because only reliable tools move #science forward 🚀
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Reposted by Karina Kaczmarek-Hajek, PhD
Roman Tsukanov @romantsukanov.bsky.social · 27/08/2026
New publication alert 🚨 Our Nano Letters paper on self-blinking dyes with nanobodies for robust SMLM is now Open Access! pubs.acs.org/nalefd/artic... Commercial Nb–JF635b probes are available from @nanotag.bsky.social. Joint effort of amazing collaborators, inspired by the great @opazo.bsky.social
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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 27/08/2026
🔬🫧🧠 NEW Product Alert: Targeting luminal #Syt1 with flexible readouts powered by the #ALFAsystem! Meet our new sdAb anti-Syt1 Luminal Domain ALFA-tag fusion (N4843) – combine it with different #NbALFA -based reagents for fluorescence imaging or EM. 🔗 Explore more: nano-tag.com/product/sdab...
Inside synaptic vesicle lumen sdAb anti-Syt1 Luminal Domain ALFA-tag fusion (N4843, NanoTag Biotechnologies) binds to the luminal domain of Syt1. The ALFA-tag is detected by NbALFA coupled to application-specific labels, e.g. fluorophores or nanogold particles.
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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 14/08/2026
🔬 Exciting to see our sdAb tools in new #microscopy approaches! In recent Nature Biotechnology @yongdengzhang.bsky.social & colleagues introduce me4Pi-SMLM, achieving near-isotropic 3D localization precision of 2–3 nm. Happy to see our anti-ALFA, anti-GFP & anti-RFP sdAbs used in this work! #SMLM
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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 17/07/2026
🔬🫧💚 #FluorescenceFriday New Product – sdAb anti-Syt1 Luminal Domain #pHluorin fusion! A pH-sensitive reporter combining our sdAb anti-Syt1 Luminal Domain with superecliptic pHluorin for real-time monitoring of #SynapticVesicles exo-endocytic cycling. ♻️ #Neuroscience #Nanobody #Synaptotagmin1
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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 08/07/2026
👋 ¡Hola from Barcelona! 🇪🇸☀️ Great start to #FENS2026! Visit NanoTag at Booth #43 to explore our #nanobody tools for #Immunofluorescence, #OneStepIF, #Multiplexing, #SuperResolution & #Pulldowns. Our team looks forward to meeting you!🦙🧠✨ #SingleDomainAntibodies #Neuroscience
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Reposted by Karina Kaczmarek-Hajek, PhD
Joachim Goedhart @joachimgoedhart.bsky.social · 01/07/2026
A High-Affinity Nanobody Recognizing mNeonGreen Enables Versatile Biochemical, Cellular, and in vivo Applications. by Steffen Frey and team: www.biorxiv.org/content/10.6...
mNeonGreen Selector: a pulldown resin for mNeonGreen-fusion proteins. (A) Sketch of mNG
Selector binding mNG fusion proteins. (B, C) Immunoprecipitation from E.coli lysate in the presence (B) or
absence (C) of target protein (mNG-Halo fusion, arrowhead). Proteins were eluted in boiling SDS sample
buffer. mNG Selector enables highly specific one-step capture of mNG-tagged proteins. (D) Binding assay to
evaluate the cross-reactivity of the mNG Selector to other recombinant fluorescent proteins. Shown is a figure
of the equilibrium bound fraction of each indicated fluorescent protein prior to buffer washing. (E)
Compatibility of mNG Selector to various reagents applied during binding (top panel) or during washing steps
(lower panel). mNG fluorescence is lost in the presence of 2 M Gua-HCl. The symbols below each row indicate
binding efficiency: ++: strong binding, + binding, -: no binding
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Reposted by Karina Kaczmarek-Hajek, PhD
Felipe Opazo @opazo.bsky.social · 02/07/2026
New paper! 🚀 A high-affinity anti- #mNeonGreen #nanobody turns #mNG into a #multifunctional Tag. @nanotag.bsky.social decided to openly share the #nanobody for research use. Not common, but we strongly believe well-defined reagents are key for reproducible science. We hope you find it useful! 💚
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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 02/07/2026
🇪🇸Heading to #FENS2026 in Barcelona? Visit #NanoTag at Booth #43! Discover our in-house developed #nanobody reagents for #imaging, protein analysis, and #neuroscience – from FluoTags, Smart Secondaries, rHcAbs to the ALFA System and Selector Resins 🦙🧠🔬. 💬 Stop by for a chat!
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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 30/06/2026
Heading to #FEBS2026? Stop by Booth #7! #NanoTag team will be there to discuss how our #nanobody technologies can support your research🦙🔬🧪 Explore the ALFA system, FluoTags, Smart Secondaries, rHcAbs & Selector Resins. See you in Maastricht! 👋 #sdAb #Immunostaining #Microscopy #ProteinAnalysis
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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 22/06/2026
Congratulations @syncellbiolab.bsky.social team on this impressive work! 👏 It's exciting to see IntAct-U-ExM revealing actin architectures with such remarkable detail. Happy to see our #ALFAsystem enabling robust post-expansion labeling & high-resolution visualization of complex actin networks. 🦙🔬
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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 22/06/2026
Did you know? #NanoTag provides #CRO services for custom single-domain antibody (sdAb) discovery & protein production🦙🧬🧑‍🔬 Meet our CEO at #BIO2026 in San Diego 🇺🇸 this week at the German Pavilion or get in touch via our website to discuss your project: nano-tag.com/contact/ #VHH #ProteinProduction
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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 15/06/2026
🔬🌈 We're expanding the spectral range of #abberior STAR-conjugated #nanobodies! 🤩 STAR 550 and deepRED have joined the range. Choose Smart Secondaries® and FluoTag®-X4 anti-GFP in your favorite colors and #multiplex with ease. 🦙🍩 In collaboration with @abberior.rocks team! #Microscopy #STED
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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 11/06/2026
#NanoTag is exhibiting at #BIO2026 in San Diego!🇺🇸 Meet our CEO Dr. Hansjörg Götzke at German Pavilion, booth 3335-AF, to explore how our 🦙 sdAb discovery platforms & protein engineering services advance life science and biomedical research. #SingleDomainAntibodies #ProteinProduction #CROservices
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Reposted by Karina Kaczmarek-Hajek, PhD
abberior @abberior.rocks · 05/06/2026
abberior STAR deepRED is now available as a nanobody conjugate ⭐ More spectral options, more flexibility for multicolor STED. Developed in collaboration with NanoTag Biotechnologies – now available for anti-GFP, anti-mouse and anti-rabbit Smart Secondaries®. #abberior #NanoTagBiotechnologies #STED
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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 22/05/2026
#Neurizons2026 is wrapping up today! Thanks to everyone who stopped by the #NanoTag booth to chat about single-domain antibody tools. 🔬 Big thanks to the organizers and all participants for making this event such a great opportunity to connect, share ideas, and talk #neuroscience. 🧠
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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 15/05/2026
NanoTag will be at #Neurizons2026! Visit our booth to explore #nanobody-based tools for super-resolution microscopy and protein analysis 🦙🧠🔬. 👋See you at Max Planck Institute for Multidisciplinary Sciences @mpi-nat.bsky.social #Neuroscience #SingleDomainAntibody
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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 06/05/2026
Astrocytes never fail to amaze 🫠✨ This beautiful GFAP⁺ network by Svilen Georgiev (Rizzoli Lab, UMG) was stained with our fluorescent #nanobody FluoTag®-X2 anti-GFAP, revealing an intricate, highly interconnected network of filament-rich processes 🧠 #sdAb #Microscopy #Neuroscience #Astrocytes #GFAP
Primary rat brain culture showing GFAP-positive astrocytes, stained with the fluorescent nanobody FluoTag®-X2 anti-GFAP (NanoTag #N3802). Image courtesy of Svilen Georgiev.
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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 28/04/2026
At a recent conference, Nahima Saliba @gustavssonlab.bsky.social, Rice University stopped by the #NanoTag booth and shared how much she enjoys using our secondary sdAbs #SmartSecondaries® for DNA-PAINT 🔬🎨 Faster, easier #multiplexing with a pre-mixing protocol – exactly what they’re designed for 💙
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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 17/04/2026
📣 Neuroscientists – something NEW for you! NanoTag expands its portfolio of neurobiological #rHcAbs 🧠. Nanobodies targeting #SYT1 and #CNPase are now available as Mouse or Rabbit IgG Fc fusions – ideal for ICC and IHC 🔬. Learn more 👉 bit.ly/4cKITOd #Neuroscience #RecombinantAntibody
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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 10/04/2026
📄 A recent study in #ACSNano by @heilemannlab.bsky.social shows that 🔬STED microscopy with DNA-fluorophore labels enables intensity-based molecular counting. Happy to see our #nanobodies 🦙 FluoTag®-X4 anti-GFP and sdAb anti-ALFA contribute to this work. Read the paper here: doi.org/10.1021/acsn...
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Reposted by Karina Kaczmarek-Hajek, PhD
NanoTag Biotechnologies @nanotag.bsky.social · 18/03/2026
Join #NanoTag team at #FOM2026 in Stockholm 🇸🇪! Visit booth #24 to see how our single-domain antibody (sdAb) technologies bring greater precision to #microscopy and more flexibility & ease to #multiplexing 🔬 See you in Stockholm! 👋 #SuperResolution #Nanoscopy #Nanobody #Immunofluorescence
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NanoTag Biotechnologies @nanotag.bsky.social · 10/03/2026
📄🦠 New publication! In collaboration with #Takeda our synthetic single-domain antibody library enabled rapid discovery of strain-specific sdAbs against SARS-CoV-2 variants, supporting vaccine identification testing. Read more in Antibodies @mdpiopenaccess.bsky.social 👉 doi.org/10.3390/anti...
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Felipe Opazo @opazo.bsky.social · 04/03/2026
New preprint out! In this study, we introduce NanoFLex, a strategy that combines #HaloTag variants with #nanobodies -based immunolabeling to enable rapid, OneStep-IF #lifetime #multiplexing. Possible due to SmartSecondaries fused to #HaloTag from @nanotag.bsky.social: www.biorxiv.org/content/10.6...
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NanoTag Biotechnologies @nanotag.bsky.social · 24/02/2026
We’re having a fantastic time at #BPS2026 in San Francisco 🌁 Last chance to stop by booth 720 to meet the #NanoTag team and learn how our nanobody-based reagents 🔬 and sdAb services 🦙 can advance your research. Thanks for the great discussions and the positive feedback on our #sdAb technologies💙!
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NanoTag Biotechnologies @nanotag.bsky.social · 19/02/2026
Did you know #multiplex IF can be done in just one incubation step? ❌ No sequential staining ❌ No host-species limits ❌ No secondary-induced clustering 🔬Pre-mix your favourite primary antibodies (even same-species) with Smart Secondaries® #nanobody & apply directly to your sample for OneStep IF.🤩
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NanoTag Biotechnologies @nanotag.bsky.social · 13/02/2026
Neuroscientists - looking for a high-affinity gift?💝 Inside the postsynapse, proteins crowd the show PSD95’s the star - but hard to know. IgG antibodies knock, but can’t get through Too bulky to reach the target they pursue. But FluoTag®-X2 anti-PSD95 slips right in Binds PSD95. Precise within.
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NanoTag Biotechnologies @nanotag.bsky.social · 12/02/2026
Heading to #BPS2026 in San Francisco? Visit NanoTag team at booth 720 to explore our single-domain antibody #sdAb tools: ALFA® System, FluoTags®, Smart Secondaries® & Selector Resins. See you! 👋 @biophysicalsoc.bsky.social #Nanobody #Multiplexing #SuperResolution #Microscopy #AffinityPurification
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NanoTag Biotechnologies @nanotag.bsky.social · 03/02/2026
💬 Thank you, Bhanu Prakash Potlapalli, for the great feedback on the ALFA® system! 😊 Applied in a CRISPR–dCas9 DNA imaging approach for efficient labeling of repetitive DNA sequences and live-cell imaging of plant telomeres doi.org/10.1093/jxb/... Explore #ALFAsystem 👉 nano-tag.com/technology/t...
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NanoTag Biotechnologies @nanotag.bsky.social · 28/01/2026
Did you know? #NanoTag offers custom single-domain antibody (sdAb) discovery services, including alpaca immunization🦙and synthetic library–based approaches🧬backed by over a decade of experience in the field 💪. Discuss your project with our experts: nano-tag.com/contact/ #CRO #Nanobody #VHH #sdAb
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NanoTag Biotechnologies @nanotag.bsky.social · 21/01/2026
🔬 Join Imaging Bites seminar 28 Jan at 12:30 at @kingscollegelondon.bsky.social. Prof. Dr. Felipe Opazo @opazo.bsky.social presents #OneStep-IF, a simplified approach to highly #multiplexed IF using same-species primaries and Smart Secondaries® premixes. Register here: www.kcl.ac.uk/events/imagi...
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NanoTag Biotechnologies @nanotag.bsky.social · 11/12/2025
#CellBio2025 in Philadelphia has come to a close! Thanks to everyone who stopped by the #NanoTag booth 🦙 – great conversations and insights all around. We were excited to hear how our sdAb tools are driving your new discoveries 📄👏 #SingleDomainAntibody #ALFAsystem #SmartSecondaries
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NanoTag Biotechnologies @nanotag.bsky.social · 06/12/2025
What most people don’t see is the behind-the-scenes effort- not magic, just #teamwork. Karina & Steffen have #NanoTag booth ready for #CellBio2025 in Philadelphia. Starting Sunday, discover how our single-domain antibody solutions can support your research🔬🦙. See you ascbiology.bsky.social 👋
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NanoTag Biotechnologies @nanotag.bsky.social · 25/11/2025
Attending #CellBio2025 in Philadelphia Dec 6–10? Visit #NanoTag team at booth #1117 to explore our #sdAb technologies & ready-to-use reagents: ALFA® System, FluoTags®, Smart Secondaries®, Selector Resins. See you there! 👋 @ascbiology.bsky.social #Nanobody #CellBiology #Immunofluorescence #PullDown
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NanoTag Biotechnologies @nanotag.bsky.social · 21/11/2025
Join our CTO, Felipe Opazo @opazo.bsky.social, at #MBSJ2025 @mbsj-official.bsky.social as he presents new super-resolution #multiplexing advances powered by #SmartSecondaries®. 🔬Discover flexible, scalable and precise multi-target imaging. #Nanobody #NanoPlex #SUMPAINT #SuperResolution #Microscopy
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NanoTag Biotechnologies @nanotag.bsky.social · 06/11/2025
Have you heard of #ANGEL? Not this 😇, but ALFA Nanobody-Guided Endogenous Labeling #ANGEL - the new approach published by Wang et al. in @natchembio.nature.com Build on our ALFA® System, it enables direct fluorescent readout of #CRISPR knock-ins. 📄Full paper here: bit.ly/47XgK48
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ALFA nanobody-guided endogenous labeling - Nature Chemical Biology
Small peptide tags (like ALFA) cause minimal disruption of proteins, but they typically lack fluorescence, which would enable knockin screening. Now, antigen-stabilizing fluorescent protein-fused nano...
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NanoTag Biotechnologies @nanotag.bsky.social · 30/09/2025
🔬✨ #GEF25 Expansion Microscopy Forum in Göttingen just wrapped up! We were happy to support as sponsors and share our sdAb innovations for super-resolution #microscopy. Thanks to the organizers & all participants for an inspiring event! 👏 #ExM #SmartSecondaries #Multiplexing
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NanoTag Biotechnologies @nanotag.bsky.social · 17/09/2025
#ExM turned 10 this year - just like #NanoTag! 🎉 Join us & the super-resolution community in Göttingen for the ExM Forum! 🔬Hear from our founder Felipe Opazo @opazo.bsky.social and CSO Steffen Frey on how NanoTag’s sdAb solutions empower nanoscopy & #multiplex imaging. See you in Göttingen! #GEF25
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NanoTag Biotechnologies @nanotag.bsky.social · 04/09/2025
📣 Expanding our Halo portfolio! Introducing Halo Selector - #sdAb -based affinity resin for highly specific, easy & reliable #HaloTag fusion protein #pulldown. ✅ High affinty & capacity ✅ Ultra- low background ✅ Broad buffer compatibility ✅ Works with bound ligands Learn more: bit.ly/465BKnm
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NanoTag Biotechnologies @nanotag.bsky.social · 14/08/2025
🔬 Two weeks to go until the 14th #SMLM2025 @smlm-symposium.bsky.social! NanoTag joins as Gold Sponsor to support this unique #SuperResolution community event. Don't miss our CSO Dr. Steffen Frey talk on Smart Secondaries® (27 Aug, 14:40) or meet him at the #NanoTag booth 🦙. #Nanobody #Multiplexing
Empowering Nanoscopy with single-domain antibodies_NanoTag Biotechnologies_SMLM2025
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NanoTag Biotechnologies @nanotag.bsky.social · 01/08/2025
📄A new Nature Commun. paper by Rahman F. et al. maps nuclear organization in 3D at super-resolution using multiplexed Exchange-PAINT & HIST. 🔬 12 nuclear proteins were detected using our Smart Secondaries® #nanobody 🦙technology, customised by @massivephotonics.bsky.social 🎨. doi.org/10.1038/s414...
doi.org
Mapping the nuclear landscape with multiplexed super-resolution fluorescence microscopy - Nature Communications
The cell nucleus coordinates diverse functions. Here, the authors utilise multiplexed super-resolution imaging to study how nuclear proteins are distributed relative to each other from the micro to th...
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NanoTag Biotechnologies @nanotag.bsky.social · 08/07/2025
🎨 Precision and Molecular imaging need a broad palette. FluoTags® are fluorescent #sdAbs/ #nanobodies available in Alexa, Atto, AbberiorSTAR, JF635b, IRDyes & more for #Confocal, #STED, #STORM, and #NIR imaging. Custom dye? No ProbLama! nano-tag.com/shop/ #Microscopy #Nanobody #Multiplexing
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NanoTag Biotechnologies @nanotag.bsky.social · 28/05/2025
🤹‍♀️ Still juggling primary antibodies from different species? Still stuck in sequential staining? ⏱️Switch to Smart Secondaries®🦙: ✅OneStep-IF ✅Same-species #multiplexing ✅Higher precision & reproducibility! Choose smart 👉 bit.ly/3UZxRMI #SecondaryNanobody #Microscopy #Immunofluorescence
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NanoTag Biotechnologies @nanotag.bsky.social · 13/05/2025
A new study by @jungmannlab.bsky.social presents #SPINNA, a model-based tool for quantifying protein stoichiometry, oligomerization state, and spatial arrangement from super-resolution data.🔬For precise labeling of #GFP & #mCherry fusion proteins researchers used our #nanobodies 😊 📄 bit.ly/4jTHfes
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Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution - Nature Communications
Extracting quantitative information on biomolecular oligomerisation with high resolution remains a significant challenge. Here, the authors propose SPINNA, a framework that compares nearest-neighbour ...
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