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Justus Kebschull

@justuskebschull.bsky.social
646 followers 139 following 28 posts

Neuroscientist of sorts. Head of the Kebschull Lab at Johns Hopkins BME. www.kebschull-lab.org

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Reposted by Justus Kebschull
Manjari Anant @mmganant.bsky.social · 22/09/2026
Really excited to share this work with the community! Big thanks to @justuskebschull.bsky.social and @jef.works, as well as our lab members and collaborators, for their support and fun discussions throughout this project!
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Justus Kebschull @justuskebschull.bsky.social · 21/09/2026
Its basal part is derived from the evolutionarily expanding early RL, like the other CN. The higher-order part is uniquely derived from the late RL and doesn’t recruit inhibitory IO-projection neurons, forming an entirely different cerebellar circuit.
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Justus Kebschull @justuskebschull.bsky.social · 21/09/2026
In the 1-to-2-to-3 nucleus evolution scheme, we often think of the medial nucleus as the oldest, most basal nucleus, but it also performs higher-order functions in mammals. Here, we resolve this by finding that the medial nucleus has a dual developmental and likely evolutionary origin!
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Justus Kebschull @justuskebschull.bsky.social · 21/09/2026
Nucleus-invariant inhibitory neurons integrate into these territories by P4.
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Justus Kebschull @justuskebschull.bsky.social · 21/09/2026
In mice, excitatory CN neurons establish all adult nuclear territories by E15.5.
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Justus Kebschull @justuskebschull.bsky.social · 21/09/2026
The cerebellar nuclei expanded from 1 to 2 to 3 nuclei across vertebrates. We find that it is the early RL that changes to produce these new nuclei. Making a new nucleus is a two-step process: Make a new progenitor, then sort its offspring into spatially separate regions.
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Cedric Boeckx @cedricboeckx.bsky.social · 20/09/2026
Excellent preprint from @justuskebschull.bsky.social’s lab showing how new cerebellar nuclei arise by excitatory progenitor diversification in the early rhombic lip 🧪🧠🧬 www.biorxiv.org/content/10.6...
biorxiv.org
Evolution of new cerebellar nuclei by excitatory progenitor diversification in the early rhombic lip
The cerebellar nuclei, the output regions of the cerebellum, have evolved via repeated duplication of a conserved cell type set to produce different numbers of nuclei across species. Here, we investig...
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Justus Kebschull @justuskebschull.bsky.social · 21/09/2026
NEW PREPRINT! An evo-devo look at how new cerebellar nuclei are made. Spoiler: it’s all driven by region-specific excitatory progenitors in the early rhombic lip; inhibitory neurons fill in later. If you care about brain evolution or cerebellar development, check it out: tinyurl.com/CNdevelopment
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Steffen Wolff @sbe-wolff.bsky.social · 19/08/2026
PREPRINT ALERT! Sophie and Alex, amazing PhD students from the Wolff and Anastasidis labs, teamed up to venture into Microbubble-enhanced focused ultrasound (MB-FUS) and took a new, multimodal approach to test its safety in-depth. (1/10) 🧪 #neuroskyence #neuroscience www.biorxiv.org/content/10.6...
biorxiv.org
Neurobehavioral effects of focused ultrasound-mediated blood-brain barrier opening
Microbubble-enhanced focused ultrasound (MB-FUS) enables noninvasive blood-brain barrier (BBB) opening to improve the delivery of drugs and other therapeutics to the brain, supporting more effective t...
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Justus Kebschull @justuskebschull.bsky.social · 04/06/2026
POINTseq, now out in Neuron after peer review. Read it. There are barcodes, viruses, and dopamine. Congrats to @hyopilkim.bsky.social on leading our lab's first published paper!
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Justus Kebschull @justuskebschull.bsky.social · 24/05/2026
For all cerebellum and cerebellar nuclei aficionados: A cre line specifically tagging the large, excitatory B-cells in the mouse cerebellar nuclei: www.biorxiv.org/content/10.6... Lmk if you want to use it!
biorxiv.org
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Justus Kebschull @justuskebschull.bsky.social · 17/05/2026
Teamwork by Huihui Qi, @mmganant.bsky.social, and @dylanzgonzo.bsky.social
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Justus Kebschull @justuskebschull.bsky.social · 17/05/2026
New from the lab: BARseq3! Barcodes + high efficiency spatial transcriptomics and translatomics in the same cells, or just a nice spatial transcriptomics assay. All in a modular, expandable system to enable truly multimodal measurements. Check it out: www.biorxiv.org/content/10.6...
biorxiv.org
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Cedric Boeckx @cedricboeckx.bsky.social · 06/05/2026
“Specific expansion of motor cortical projections in a singing mouse” Great new work by @arkarupbanerjee.bsky.social E. Isco @cliffscience.bsky.social @xmikezheng20.bsky.social @tonyzador.bsky.social & colleagues on the neural basis of vocal dexterity, now out in @nature.com 🧪🧠
nature.com
Specific expansion of motor cortical projections in a singing mouse - Nature
High-throughput barcoded neuroanatomy of two closely related rodent species with divergent vocalizations reveals differences in long-range projection motifs in the brain that may s...
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Journal of Open Source Software @joss-openjournals.bsky.social · 14/01/2026
Just published in JOSS: 'DART: A GUI Pipeline for Aligning Histological Brain Sections to 3D Atlases and Automating Laser Microdissection' doi.org/10.21105/joss.09109
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Arkarup Banerjee @arkarupbanerjee.bsky.social · 19/11/2025
As a longtime fan of cool papers in @currentbiology.bsky.social, I am really thrilled to see this out! This study sets the stage for understanding the origins of novel (vocal) behaviors. Big shout out to the main architects of this work @xmikezheng20.bsky.social and @cliffscience.bsky.social
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Paul Masset @paulmasset.bsky.social · 19/11/2025
I am recruiting graduate students for the experimental side of my lab @mcgill.ca for admission in Fall 2026! Get in touch if you're interested in how brain circuits implement distributed computation, including dopamine-based distributed RL and probabilistic representations.
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Justus Kebschull @justuskebschull.bsky.social · 15/11/2025
If you are at #SfN2025, don't miss Huihui's poster tomorrow (Sunday) morning at MM6 (PSTR095.13)! He has developed methods for barcoded connectomics across many vertebrates, from frogs to NHPs, and is comparing the single-cell anatomy of the song systems of zebra finches and parrots!
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Justus Kebschull @justuskebschull.bsky.social · 09/09/2025
Annoyed at how difficult MAPseq dissections can be? Check out the new preprint from Kebschull undergrad Rishi Koneru and grad student @mmganant.bsky.social: www.biorxiv.org/content/10.1...
biorxiv.org
DART: A GUI Pipeline for Aligning Histological Brain Sections to 3D Atlases and Automating Laser Microdissection
The precise dissection of anatomically defined brain regions is the basis of many workflows in neurobiology. Traditionally, brain regions of interest are defined by visual inspection of tissue section...
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Justus Kebschull @justuskebschull.bsky.social · 19/07/2025
Want to learn everything there is to know about barcoded connectomics, brain clearing, and modern neuroanatomy? There is still time to apply for the CSHL High Throughput Neuroanatomy course! Stipends are available. meetings.cshl.edu/courses.aspx...
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Justus Kebschull @justuskebschull.bsky.social · 07/07/2025
I hope you all have fun digging into this and using POINTseq and MAPseq2 for your own favorite circuits!
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Justus Kebschull @justuskebschull.bsky.social · 07/07/2025
And even when neurons innervate the same kind of regions, they can do so in different ways. VTA and SNc DA neurons projecting to the dorsal striatum project fundamentally differently. SNc neurons innervate more subregions and broadcast randomly to these regions, while VTA neurons are highly specific
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Justus Kebschull @justuskebschull.bsky.social · 07/07/2025
This large number of clusters is not an effect of splitting a stochastically wired system. Co-innervation patterns and projection motifs are often far from the null model of independent projection choice.
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Justus Kebschull @justuskebschull.bsky.social · 07/07/2025
We find over 30 connectomic cell types, way more than would have been expected from transcriptomic cell types. Overall, our data agrees nicely with what we know, but boy, is there more structure to this system. Maybe easier to see from the dot plots.
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Justus Kebschull @justuskebschull.bsky.social · 07/07/2025
To showcase POINTseq's utility, we then applied it to the midbrain dopaminergic system, mapping >3,800 DA neurons in the VTA and SNc.
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Justus Kebschull @justuskebschull.bsky.social · 07/07/2025
We validated POINTseq in the mouse motor cortex using two cre lines and AAVretro-cre to define cell types of interest.
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Justus Kebschull @justuskebschull.bsky.social · 07/07/2025
This works all over the brain
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Justus Kebschull @justuskebschull.bsky.social · 07/07/2025
POINTseq achieves cell type specific barcoding by pseudotyping barcoded Sindbis virus with the structural proteins of Alphavirus CHIKV, and overexpressing CHIKV cellular receptor Mxra8 in target cells. Mxra8 isn't normally expressed in mouse neurons, enabling cell type specific infection.
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Justus Kebschull @justuskebschull.bsky.social · 07/07/2025
Finally have time to add some more meat here. MAPseq2 is what it says on the tin. Better and cheaper. Use this going forward for anything involving MAPseq, including BARseq, BRICseq, and ConnectID!
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Arkarup Banerjee @arkarupbanerjee.bsky.social · 26/06/2025
Next-generation of barcoded projection mapping techniques: MAPseq2 and POINTseq! Congratulations @justuskebschull.bsky.social and team.
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Justus Kebschull @justuskebschull.bsky.social · 26/06/2025
www.biorxiv.org/content/10.1...
biorxiv.org
Mapping the projectional architecture of the mouse midbrain dopaminergic system using cell type-specific barcoding
Brain-wide neural circuits are formed by the diverse axonal branching patterns of many individual neurons. Here we introduce POINTseq (projections of interest by sequencing), a high-throughput and use...
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Justus Kebschull @justuskebschull.bsky.social · 26/06/2025
www.biorxiv.org/content/10.1...
biorxiv.org
MAPseq2: a sensitive and cost-effective barcoded connectomics method
The barcoded connectomics tool MAPseq enables highly multiplexed projection mapping of individual neurons by translating neuroanatomy into a DNA sequencing problem. Here we present MAPseq2, a user-fri...
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Justus Kebschull @justuskebschull.bsky.social · 26/06/2025
Excited to share the first 2(!) preprints from the Kebschull Lab. The amazing @hyopilkim.bsky.social developed MAPseq2 & POINTseq and used them to map the dopaminergic cells of VTA and SNc. tl;dr MAPseq2=10x cheaper, 4x better; POINTseq=easy cell type specific barcoding; DA neurons=very cool.
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Paul Masset @paulmasset.bsky.social · 04/06/2025
Our work with Pablo Tano, @hyunggoo-kim.bsky.social Athar Malik, Alexandre Pouget and @naoshigeuchida.bsky.social exploring how dopamine neurons could enable multi-timescale reinforcement learning in the brain is out in @nature.com www.nature.com/articles/s41...
nature.com
Multi-timescale reinforcement learning in the brain - Nature
Individual dopaminergic neurons encode future rewards over distinct temporal horizons.
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Klingenstein Philanthropies @klingensteinorg.bsky.social · 02/06/2025
Thank you to @xinjin.bsky.social and @justuskebschull.bsky.social for presenting their research at the recent Klingenstein Fellowship Awards in Neuroscience Conference. 📷 : SimonProPhoto
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Joaquina Delas @joadelas.bsky.social · 08/05/2025
Fantastic opportunity to move to Europe, and yes, it includes the UK! But applicants can be from any nationality. Our team at UCL is still growing. Get in touch if any of our work sounds interesting. delaslab.com
delaslab.com
Delas Lab
at University College London. Decoding cis regulatory control during development.
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Stand Up for Science! @standupforscience.net · 12/02/2025
Welcome to the Bluesky account for Stand Up for Science 2025! Keep an eye on this space for updates, event information, and ways to get involved. We can't wait to see everyone #standupforscience2025 on March 7th, both in DC and locations nationwide! #scienceforall #sciencenotsilence
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Cell & Systems Biology (CSB) @uoftcellsysbiol.bsky.social · 10/02/2025
On Friday, Feb 14 ❤️, we will welcome Prof Justus Kebschull @justuskebschull.bsky.social of Johns Hopkins Biomedical Engineering for his talk on “Understanding brain function and evolution with barcodes and sequencing”. Happening at 11am in RW432. See you there!
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Rick Huganir @rhuganir.bsky.social · 07/02/2025
This is the end of science as we know it if it stands—15% IDCs for all NIH Grants. grants.nih.gov/grants/guide...
grants.nih.gov
NOT-OD-25-068: Supplemental Guidance to the 2024 NIH Grants Policy Statement: Indirect Cost Rates
NIH Funding Opportunities and Notices in the NIH Guide for Grants and Contracts: Supplemental Guidance to the 2024 NIH Grants Policy Statement: Indirect Cost Rates NOT-OD-25-068. OD
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IDDjobs @iddjobs.org · 20/01/2025
Postdoc (Baltimore, United States) Viral Phylodynamics & Neuroscience with @justuskebschull.bsky.social @rezakalhor.bsky.social More details: iddjobs.org/jobs/johns-hopkins-post…
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IDDjobs @iddjobs.org · 20/01/2025
Bioinformatician (Baltimore, United States) Viral Phylodynamics & Neuroscience with @justuskebschull.bsky.social @rezakalhor.bsky.social More details: iddjobs.org/jobs/hopkins-bioinforma…
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Seth Blackshaw @sethblackshaw.bsky.social · 08/01/2025
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Richard Sever @richardsever.bsky.social · 21/12/2024
Many academics point to bioRxiv as “the one thing improving science publishing”. If so, the one thing you all can do is persuade colleagues to submit and make this a norm. 1/2
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Denis Wirtz @deniswirtz.bsky.social · 17/12/2024
Hopkins is recruiting 30 Bloomberg Distinguished Professors in AI + X (X = astrophysics, cancer, neuroscience, genomics, social sciences, etc.) hub.jhu.edu/2024/12/17/d... 22 of these 30 will be organized in clusters that were selected after rigorous external review. The list of clusters is:
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Alison L Hill @alisonhill.bsky.social · 11/12/2024
Experience in viral phylodynamics? Check out this awesome opportunity to use those tools to understand brain structure 🧠🦠. We'll be next door neighbors and you can join the amazing infectious disease dynamics and neuroscience communities at Johns Hopkins
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Justus Kebschull @justuskebschull.bsky.social · 01/12/2024
We are still looking to recruit a postdoc on (what I think) is a most exciting project!
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Justus Kebschull @justuskebschull.bsky.social · 18/11/2024
The Kebschull and @rezakalhor.bsky.social labs are recruiting a joint postdoc for an exciting project at the interface of phylodynamics and neuroscience. Come join us at JHU BME. Spread the word, and contact us if you are interested. #hiring 🧪🧠. More on www.kebschull-lab.org and below.
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Justus Kebschull @justuskebschull.bsky.social · 15/11/2024
Hello there! We are the Kebschull lab at Johns Hopkins University and love brains, barcodes, viruses, and sequencing. We develop new technology and work on a zoo of animals to understand how brains change over evolution and an animal's lifetime. Check out our lab at www.kebschull-lab.org 🧠🧪
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