Ben jij gefascineerd door het immuunsysteem? Wil je je verdiepen in de wereld van immuuncelproteasen en hun rol bij inflammatie? Wij zoeken een sterk gemotiveerde persoon om ons onderzoeksteam te vers...

Investigating proteases and protease inhibitors produced and secreted by primary cells is complicated by the presence of exogenous ones in cell culture medium containing fetal bovine serum. With this protocol, we generate human monocyte-derived macrophages in the absence of exogenous proteases and protease inhibitors by using a serum replacement for the final 24 h of macrophage culture. We describe steps for seeding and differentiating monocytes into macrophages. We then detail procedures for ma...

Poorly performing antibodies have plagued biomedical sciences for decades. Several fresh initiatives hope to change this.

Natural sciences; Biological sciences; Immunology; Immune response