iiinova — where your digital self keeps evolving

Heilemann Lab @heilemannlab.bsky.social · 14/10/2026

🔬 super-resolution DNA-PAINT in bacteria requires a labelling method => great work by the Christoph Spahn lab @michriscopy.bsky.social @uni-wuerzburg.de 👉🏻 www.biorxiv.org/content/10.6...

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Heilemann Lab @heilemannlab.bsky.social · 25/09/2026

#FluorescenceFriday #STED pure and real 8-color STED microscopy & happy TxIF to everyone 🌴

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Heilemann Lab @heilemannlab.bsky.social · 15/09/2026

Do peptide–MHC I complexes cluster on the cell surface? We counted them, one by one. Picomolar soluble TCRs + #DNAPAINT on primary human dendritic cells: pMHC I is monomeric. No nanoclusters. What is regulated instead: surface density, and where complexes sit. doi.org/10.1073/pnas.2615838123

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Heilemann Lab @heilemannlab.bsky.social · 17/08/2026

🚨 2x PhD opportunities: single-molecule/super-resolution microscopy & image analysis of membrane proteins 🔬(DM for details) 👉🏻 tinyurl.com/5dt7ncby 👉🏻 tinyurl.com/5e52dth2 🙏 Dear colleagues & friends, pls distribute - Thank you! 🙏 @crc1507.bsky.social imol.uni-frankfurt.de @goetheuni.bsky.social

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Heilemann Lab @heilemannlab.bsky.social · 01/08/2026

🔬Check out this clever approach for 3D SMLM by Yoav Shechtman @technion.bsky.social and @gustavssonlab.bsky.social ... stunning images! 👉🏻 doi.org/10.64898/202...

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Heilemann Lab @heilemannlab.bsky.social · 12/05/2026

Thanks for this great surprise, Soo! 🙏

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Heilemann Lab @heilemannlab.bsky.social · 12/05/2026

🎉 Congratulations, Dr. Soohyen Jang! 🎉 Soo established fluorophore-binding enzymes for single-molecule super-resolution microscopy 🙏 great collaborations @juliankompa.bsky.social @kjohnsson.bsky.social Yoav Shechtman @technion.bsky.social &🙏 grad school @imprs-cbp.bsky.social @goetheuni.bsky.social

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Heilemann Lab @heilemannlab.bsky.social · 25/04/2026

#FluorescenceFriday contribution: 3D DNA STED of NPC, entire nucleus of a U-2 OS cell. 🔬

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Heilemann Lab @heilemannlab.bsky.social · 30/03/2026

Great work, congratulations! Is there possibly a connection between the 3' binding site of TDP-43 and the 3' loop of NEAT1_2 in PS? 👉🏻 www.nature.com/articles/s41...

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Heilemann Lab @heilemannlab.bsky.social · 21/03/2026

Molecule counting with STED🔬= fast & 3D & entire cells 👉🏻 we used DNA-fluorophore labels, evaluated intensity-based molecule counting on DNA origami, and measured EGFR dimerization in cells. Great work 💪 from Laurell, Ash and @yunqingli.bsky.social 👉🏻 now out in ACS Nano 👉🏻 doi.org/10.1021/acsn...

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Heilemann Lab @heilemannlab.bsky.social · 16/03/2026

DNA-PAINT with photocaged DNA labels = PhotoPAINT🔬. Why? No need for washing or mixing 👉🏻 higher accuracy in DNA-PAINT & other super-res methods. Fantastic collab with Alex Heckel & driven by the 'magic team' Nina Kaltenschnee and Marina Dietz 💪 doi.org/10.1002/anie... @goetheuni.bsky.social

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Heilemann Lab @heilemannlab.bsky.social · 04/02/2026

🔬we have been playing with 'an old tool from (DNA) chemistry' 🧪 and used that for DNA-based confocal/super-res microscopy ... these are the last recordings for the revision and were recorded last night ... 🙏 thanks to R1 for this suggestion:

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Heilemann Lab @heilemannlab.bsky.social · 20/12/2025

A cool thing was the FRET-based diffusion analysis that allowed a separate analysis of MET receptor monomers and dimers👇🏻

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Heilemann Lab @heilemannlab.bsky.social · 20/12/2025

Receptor tyrosine kinases bind ligands and (often) dimerize. Yet how long, and how does that change their mobility? Using single-molecule FRET in living cells, we found that MET dimers 'socialize' for ~1 second while lowering lateral diffusion and confinement 👉🏻 doi.org/10.1002/smll...

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Heilemann Lab @heilemannlab.bsky.social · 28/11/2025

We found how paraspeckles grow, how the scaffolding RNA is organized (using a spherical harmonics approach), and measured the coarse structure of the RNA in the particles using multi-site labeling inspired by DNA-PAINT. media.springernature.com/full/springe...

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Heilemann Lab @heilemannlab.bsky.social · 28/11/2025

We developed a 'smart STED microscope' to detect rare objects/events in cells, and unraveled the 3D morphology of a nuclear membraneless organelle (paraspeckle). Amazing collaboration with Sjoerd Stallinga, Bernd Rieger (TU Delft) and @mixmue.bsky.social 🙏 (1/3) www.nature.com/articles/s41...

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Heilemann Lab @heilemannlab.bsky.social · 09/10/2025

If you happen to be at Seeing is Believing in Heidelberg #EESImaging: Soohyen and Yunqing are presenting some of our recent work 🔬today (talk) and tomorrow (poster 116):

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Heilemann Lab @heilemannlab.bsky.social · 07/10/2025

It was a tremendous pleasure to have @zhixingchen2.bsky.social visiting us today in Frankfurt and listen to his great science on developing photostable and gentle fluorophores for microscopy. Thank you, Zhixing, for this inspiring seminar! 🙏 @goetheuni.bsky.social @scale-cluster-ffm.bsky.social

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Heilemann Lab @heilemannlab.bsky.social · 25/09/2025

Please visit Ashwin at his poster P84 in today's poster session at the PicoQuant Workshop #WS30 and learn about long-term (>6 hours!) of continuous STED microscopy🔬 in living cells @picoquant.bsky.social @sfb1177.bsky.social (and here is Ash's paper on this work 👉🏻 doi.org/10.1002/smsc...)

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Heilemann Lab @heilemannlab.bsky.social · 23/09/2025

Soo on stage presenting self-labeling enzymes with weak-affinity fluorophore labels for (1) high-density live SMLM using DeepSTORM (doi.org/10.1016/j.bp...) and (2) molecular counting ... great collaboration with @kjohnsson.bsky.social @juliankompa.bsky.social 🙏 #PQ30 @picoquant.bsky.social

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Heilemann Lab @heilemannlab.bsky.social · 23/09/2025

The 30th (!) "Single Molecule Spectroscopy and Super-resolution Microscopy" workshop has just been opened by @raierdmann.bsky.social @picoquant.bsky.social A great line-up of speakers & posters 🔬 #PQ30

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Heilemann Lab @heilemannlab.bsky.social · 29/08/2025

And we are done --- #SMLMS2025 is over, and it was great. Thank you Uli & Ulrike & all the helpers 👍, and thanks to everyone joining this event 🙏 ... safe travels home & see you soon!

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Heilemann Lab @heilemannlab.bsky.social · 29/08/2025

Helge Ewers @ewerslab.bsky.social takes us on an exciting journey into light-controlled release of single proteins in living cells enabling cool new cell biology such as light-controlled signalling studies #SMLMS2025

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Heilemann Lab @heilemannlab.bsky.social · 29/08/2025

Next on stage is Mahipal Ganji from the IIT Bangalore @ganjilab.bsky.social shares great work on stacking interactions in DNA/RNA and multplexed-DNA PAINT to measure nuclear ultrastructure #SMLMS2025

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Heilemann Lab @heilemannlab.bsky.social · 29/08/2025

Hannah Kim from the Lakadamyali lab at UPenn @melikel.bsky.social reports an analysis pipeline for cell-type specific chromatin analysis #SMLMS2025

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Heilemann Lab @heilemannlab.bsky.social · 29/08/2025

Now on stage is Yoav Shechtman from Technion @technion.bsky.social reporting phase mask engineering for 3D SMLM and one-click image reconstruction using a CNN #SMLMS2025

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Heilemann Lab @heilemannlab.bsky.social · 29/08/2025

Next on stage is Remi Galland from @iins-bordeaux.bsky.social reporting a novel platform for in-depth super-resolution imaging #SMLMS2025

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Heilemann Lab @heilemannlab.bsky.social · 29/08/2025

Estibaliz Gomez de Marisca @gomez-mariscal.bsky.social from Lisbon going live and sharing great work on AI-empowered microscopy #SMLMS2025 and accessible AI

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Heilemann Lab @heilemannlab.bsky.social · 29/08/2025

Anna-Karin Gustavsson @gustavssonlab.bsky.social from Rice University starts off day #3 of #SMLMS2025 with impressive work on whole-cell 3D single-molecule imaging.

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Heilemann Lab @heilemannlab.bsky.social · 28/08/2025

Ran Huo from TU Delft (Kristin Grußmayer lab) reports very nice work on fast super-resolution microscopy using DNA labels for SOFI #SMLMS2025 www.tudelft.nl/en/faculty-o...

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Heilemann Lab @heilemannlab.bsky.social · 28/08/2025

Next on stage is Jonas Ries @jonasries.bsky.social from Max Perutz Labs in Vienna sharing great work on a MINFLUX simulator that helps in designing experiments 👉🏻 SimuFLUX #SMLMS2025

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Heilemann Lab @heilemannlab.bsky.social · 28/08/2025

Ever wondered where cargo travels through the central channel of the NPC? Siegfried Musser lab @musser-lab.bsky.social measured that in living cells using MINFLUX. Fascinating data! #SMLMS2025

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Heilemann Lab @heilemannlab.bsky.social · 28/08/2025

We are starting the afternoon session with Steffen Sahl from MPI for Multidisciplinary Sciences in Göttingen @mpi-nat.bsky.social sharing exciting MINFLUX data reporting intra-molecular distances and conformations #SMLMS2025

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Heilemann Lab @heilemannlab.bsky.social · 28/08/2025

We are so happy to have Zaida Luthey-Schulten here at #SMLMS2025 as a keynote speaker. Zaida is the Director of the NSF Science & Technology Center of Quantitative Cell Biology and Endowed Professor at UIUC. She pioneered 4D modelling of an entire cell integrating experimental data. So inspiring!

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Heilemann Lab @heilemannlab.bsky.social · 28/08/2025

Johannes Stein @jmstein.bsky.social from Harvard/Wyss reports impressive work on imaging the nuclear ultrastructure of cells using cryosections and DNA-PAINT #SMLMS2025

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Heilemann Lab @heilemannlab.bsky.social · 28/08/2025

The next presentation is by Qingru Li from TU Delft reporting really 'cool work' on cryo-4Pi single-molecule localization microscopy #SMLMS2025

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Heilemann Lab @heilemannlab.bsky.social · 28/08/2025

Johannes Hohlbein @hohlbeinlab.bsky.social from Wageningen presenting his great work on the dynamics of the E. coli replication initiator DnaA #SMLMS2025

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Heilemann Lab @heilemannlab.bsky.social · 28/08/2025

Jarno Mäkelä @jmakela.bsky.social from Aalto University shares his great work on single-molecule dynamics of heat shock proteins in E. coli #SMLMS2025 (unpublished work, so no images here)

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Heilemann Lab @heilemannlab.bsky.social · 28/08/2025

First on stage is Maja Klevanski @majaklevanski.bsky.social from Heidelberg University presenting her great work on multiplexed super-resolution imaging of proteins and glycans in tissue #SMLMS2025

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Heilemann Lab @heilemannlab.bsky.social · 28/08/2025

Day #2 of #SMLMS2025 is taking off right now and introduced by Uli Kubitscheck👇🏻

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Heilemann Lab @heilemannlab.bsky.social · 27/08/2025

Xavier Darzacq is our keynote speaker of today and takes us on an amazing journey through the cell nucleus with amazing single-molecule biology #SMLMS2025 www.tjian-darzacq.mcb.berkeley.edu

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Heilemann Lab @heilemannlab.bsky.social · 27/08/2025

Next on stage is Tanja Menche from @heilemannlab.bsky.social reporting single-molecule imaging of the T-cell receptor in cells using quantitative DNA-PAINT #SMLMS2025 👏

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Heilemann Lab @heilemannlab.bsky.social · 27/08/2025

We are heading on with Laura Jaekel from the Endesfelder Lab @uendesfelder.bsky.social reporting EVE, a 'cool tool' to analyze event-based localization data #SMLMS2025 👏

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Heilemann Lab @heilemannlab.bsky.social · 27/08/2025

Second session starts off with Johan Elf elflab.icm.uu.se sharing his great work on imaging the dynamics of the bacterial chromosome #SMLMS2025

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Heilemann Lab @heilemannlab.bsky.social · 27/08/2025

Dominik Wöll (RWTH Aachen) is next on stage, sharing his great work on nano-scale SMLM imaging of soft nanoparticles #SMLMS2025

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Heilemann Lab @heilemannlab.bsky.social · 27/08/2025

Next on stage is Eduard Unterauer @eduardunterauer.bsky.social from @jungmannlab.bsky.social reporting spatial proteomics with DNA PAINT #SMLMS2025

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