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Akaash Kumar

@akaashkumar.bsky.social
685 followers 723 following 19 posts

Interdisciplinary scientist interested in technology development, microscopy and biological sciences. Multispectral live cell imaging of membrane trafficking. MRC LMB, Cambridge, UK.

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Reposted by Akaash Kumar
James Manton @jamesdmanton.bsky.social · 02/06/2026
We present: Index-agnostic oblique plane light sheet microscopy of centimetre-scale cleared tissues at subcellular resolution www.biorxiv.org/content/10.6...
Image of first two pages of manuscript.
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Reposted by Akaash Kumar
James Manton @jamesdmanton.bsky.social · 25/06/2026
We've updated our cleared-tissue direct-view oblique plane microscopy preprint to include our latest resolution-enhancing results. Now the Nyquist sampling of our camera is no longer a limitation and so we obtain a diffraction-limited 2 μm lateral resolution! www.biorxiv.org/content/10.6...
An panel of three images showing 1) raw data, 2) bicubic interpolated data on a two-fold finer grid, 3) properly upsampled data on a two-fold finer grid, for two parallel neuronal filaments that can only be resolved as separate entities in the upsampled image.
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Akaash Kumar @akaashkumar.bsky.social · 19/03/2026
Great work from Lucas, the @simonbullock11.bsky.social lab and collaborators uncovering the relocalisation of organelles by dynein when cells experience stress! Check out the pre-print 👇🏽
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Reposted by Akaash Kumar
James Manton @jamesdmanton.bsky.social · 13/03/2026
Myself and @immunoah.bsky.social are looking to hire a postdoctoral scientist to build a next-generation high-throughput high-resolution light sheet microscope for imaging entire cleared organs/tissues and to further develop live & fixed tissue clearing methods. www.nature.com/naturecareer...
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Akaash Kumar @akaashkumar.bsky.social · 16/09/2025
🚨Publication alert🚨 My first, first-author paper is now out in @natphoton.nature.com! Our paper describes an iterative spectral unmixing algorithm and eight-channel camera-based hardware we developed enabling unmixing of low SNR live-cell data at video rates. www.nature.com/articles/s41...
nature.com
Multispectral live-cell imaging with uncompromised spatiotemporal resolution - Nature Photonics
A tree-like arrangement of dichroic mirrors and multiple cameras coupled with an iterative spectral unmixing algorithm enables multispectral imaging of live cells in up to eight spectral channels with...
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Reposted by Akaash Kumar
James Manton @jamesdmanton.bsky.social · 15/09/2025
We present a simple method to easily increase the imageable depth of an expansion microscopy gel on a typical inverted microscope ten-fold, using some carefully placed FEP film and a water dipping objective lens:
A comparison of imaging an expansion microscopy hydrogel with a water dipping objective lens, using either eye gel as an immersion medium or a combination of Immersol W, water and a thin FEP film. In both cases, the sample is supported by a thin FEP film rather than a coverglass.
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Akaash Kumar @akaashkumar.bsky.social · 20/05/2025
Today's light sheet video of actin dynamics in cytotoxic t cells... so much fun to come!
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Akaash Kumar @akaashkumar.bsky.social · 14/02/2025
Thank you very much for the feature and the article!! A nice surprise before the weekend...
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Akaash Kumar @akaashkumar.bsky.social · 07/02/2025
Congratulations @lara-kruger.bsky.social ! We wish you all the best!
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Reposted by Akaash Kumar
Derivery Lab - MRC LMB @deriverylab.bsky.social · 17/01/2025
🚨 Paper Alert 🚨 Our preprint describing Elongator as a selective microtubule polymerase that rewrites PTMs on microtubules is now published! Check it out: www.embopress.org/doi/full/10....
embopress.org
Elongator is a microtubule polymerase selective for polyglutamylated tubulin | The EMBO Journal
imageimageElongator, an established tRNA-modifying complex, can also stabilize microtubules during asymmetric cell division. This study shows that Elongator subcomplexes achieve this by binding to mic...
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Reposted by Akaash Kumar
Derivery Lab - MRC LMB @deriverylab.bsky.social · 20/01/2025
Congrats to @juliemiesch.bsky.social who was awarded a Postdoc Mobility grant by @snf-ch.bsky.social to support her research for two years. Her postdoc research will focus on the biophysical regulation of water buffering in the cell.
Picture of Julie wearing a red top and black bottoms, holding a bottle of champagne
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Reposted by Akaash Kumar
Wallace Marshall @wallaceucsf.bsky.social · 23/12/2024
Mitochondria consist of networks of cylindrical tubes, right? Not necessarily! - in our new preprint, @gavsturm.bsky.social investigates how mitochondria transiently adopt a beads-on-a-string morphology www.biorxiv.org/content/10.1...
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Reposted by Akaash Kumar
MRC Laboratory of Molecular Biology @mrclmb.ac.uk · 16/12/2024
Congrats to @akaashkumar.bsky.social who has won the PhD Award from the Cambridge Centre for Physical Biology for his work developing a novel microscopy system with @deriverylab.bsky.social & @jamesdmanton.bsky.social in @cellbiol-mrclmb.bsky.social. www.physbiol.cam.ac.uk/akaash-kumar #LMBintheNews
Akaash Kumar stands next to his multispectral imaging hardware which features several cameras. Behind him, a computer screen shows a colourful cellular image captured with his new microscopy system.
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Akaash Kumar @akaashkumar.bsky.social · 13/12/2024
Simultaneous multispectral imaging of seven organelles in live cells using our multispectral spinning disk confocal microscope! #FluorescenceFriday You can read more about this technology in our updated pre-print: www.biorxiv.org/content/10.1...
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Akaash Kumar @akaashkumar.bsky.social · 11/12/2024
Very happy that my work on developing multispectral live cell imaging technology has been recognised by the Cambridge Centre for Physical Biology! Thanks for the award!
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Reposted by Akaash Kumar
Derivery Lab - MRC LMB @deriverylab.bsky.social · 22/11/2024
In June, @akaashkumar.bsky.social and @jamesdmanton.bsky.social had their Multispectral live-cell imaging paper released on to the BioRxiv www.biorxiv.org/content/10.1...
biorxiv.org
Multispectral live-cell imaging with uncompromised spatiotemporal resolution
Multispectral imaging is an established method to extend the number of colours usable in fluorescence imaging beyond the typical limit of three or four, but standard approaches are poorly suited to li...
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Reposted by Akaash Kumar
Derivery Lab - MRC LMB @deriverylab.bsky.social · 22/11/2024
Then, in September @akaashkumar.bsky.social passed his PhD viva, becoming the latest doctor to emerge from the lab! He then won the RMS Early Career Award 2024 in October.
Akaash stands in front of some stairs, holding a bottle of champagne whilst wearing a suit.
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Reposted by Akaash Kumar
Derivery Lab - MRC LMB @deriverylab.bsky.social · 22/11/2024
Later in October, the LMB had their yearly annual symposium with Post-doc Lara Kruger winning the Steitz Prize for her talk and @akaashkumar.bsky.social winning the Perutz Prize for his PhD work!
On the bottom left is Akaash Kumar, bottom right is Lara Kruger, behind lara is Manu Derivery and behind Akaash is James Manton, all holding glasses of champagne.
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Akaash Kumar @akaashkumar.bsky.social · 22/11/2024
Some personal news: my wife and I are finally homeowners! We got the keys to our home in Cambridge today, very exciting times!
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Akaash Kumar @akaashkumar.bsky.social · 20/11/2024
Hello Bluesky! I am an engineer/scientist currently working at the MRC LMB in Cambridge UK. During my PhD I developed new imaging technologies to shed light on the dynamics of living cells e.g. here is a movie of 6 organelles in live human cancer cells. Follow for more updates! #science #biology
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