Reposted by Zenlabpasteur.bsky.socialAlfonso Martinez Arias @amartinezarias.bsky.social · 29/09/2026Intriguing set of observations on how ‘dual-aggregate gastruloids’ lead to the emergence of foregut-pharyngeal mesoderm co-development and patterning www.biorxiv.org/content/10.6... #gastruloids #MustRead 2125
Reposted by Zenlabpasteur.bsky.socialGaëlle Letort @gaellel.bsky.social · 03/09/2026The final version of the paper presenting our tool EpiCure is now online: journals.biologists.com/dev/article/... !! Thanks again to all collaborators in this projectjournals.biologists.comEpiCure (Epithelial Curation): a versatile and handy tool for curation of epithelial segmentationSummary: EpiCure is a napari plugin designed to optimise epithelia segmentation and tracking curation through automatic errors detection and ergonomic correction while being easily interoperable with ... 14219
Reposted by Zenlabpasteur.bsky.socialLevayer Lab @levayerr.bsky.social · 03/09/2026The last version of our EpiCure tool is now out in @dev-journal.bsky.social, a user friendly Napari plugin for epithelial segmentation curation. A bit thanks to @gaellel.bsky.social for carrying all along this joint department effort (check the details below). Feel free to use it and expand it ! 02311
Reposted by Zenlabpasteur.bsky.socialPablo Navarro @pablonavarrolab.bsky.social · 15/08/2026Happy to share our last work on mitotic bookmarking where we describe a new role for extensively studied TFs. Heroic effort by first author Inma over many years. genesdev.cshlp.org/content/earl...genesdev.cshlp.orgMitotic MAX bookmarking drives MYC-dependent hypertranscription at TBP-bound promoters A biweekly scientific journal publishing high-quality research in molecular biology and genetics, cancer biology, biochemistry, and related fields 23311
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 04/08/2026Two-year post-doctoral position available in zebrafish developmental neurobiology in the Bally-Cuif and Arimondo labs at Institut Pasteur, Paris, France 010
Reposted by Zenlabpasteur.bsky.socialInstitut Pasteur | 130 years of biomedical research @pasteur.fr · 27/07/2026In zebrafish, neural stem cells keep producing neurons throughout life. Pasteur scientists reveal how Notch3 signaling coordinates neighboring stem cells to maintain this balance, insights relevant to brain tumours. www.pasteur.fr/en/research-... @natcomms.nature.com #Neuroscience #StemCellspasteur.frHow Brain Stem Cells Coordinate to Produce NeuronsIn many vertebrates, brain stem cells produce neurons throughout life. A team of scientists has revealed for the first time the molecular mechanisms by which adult neural stem cells communicate with o... 0135
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 09/07/2026Huge congrats @gaellel.bsky.social !!!! We are soooo proud of you !!! 131
Reposted by Zenlabpasteur.bsky.socialCNRS @cnrs.fr · 09/07/2026#TalentsCNRS 🏅 Le CNRS décerne ses médailles de cristal et cristal collectif 2026, récompensant des femmes et des hommes ayant contribué à l’avancée des savoirs et à l’excellence de la recherche française. Félicitations 👏 👉 www.cnrs.fr/fr/actualite... 👉 www.cnrs.fr/fr/actualite... 0299
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 07/07/2026🚨 New pre-print alert! 🚨 Adult neural stem cell state and fate decisions are hidden within transcriptional heterogeneity. To find out how we decoded these heterogeneities to identify a molecularly encoded quiescence cycle, check out the pre-print on bioRxiv! doi: doi.org/10.64898/202... 12815
Reposted by Zenlabpasteur.bsky.socialLevayer Lab @levayerr.bsky.social · 06/07/2026After a quite long process, we are happy to share the last version of our article describing unexpected results regarding the coupling between local cell death and proliferation (heroic revision effort from @ralitzastaneva.bsky.social ) urldefense.com/v3/__https:/...urldefense.comA global response contributes to tissue size robustness upon local induction of apoptosisTissue resilience requires tight coupling between cell proliferation, growth, and death.1,2,3,4It is assumed to be based on compensatory proliferation… 44915
Reposted by Zenlabpasteur.bsky.socialTajbakhsh Lab @tajbakhshlab.bsky.social · 28/06/2026The importance of history, clarity on what we generate in a dish compared to the ground truth. The resourceful David Turner @gastruloids.bsky.social, initiated students on reproducible gastruloid generation and A-P extension. And now, into the 2nd week... 031
Reposted by Zenlabpasteur.bsky.socialLevayer Lab @levayerr.bsky.social · 27/03/2026Happy to share the heroic effort of @gaellel.bsky.social to reduce our segmentation pain through EpiCure (Epithelial Curation), a napari plugin easing the curation of epithelial segmentation developed with several groups of @devstempasteur.bsky.social. www.biorxiv.org/content/10.6... See more 👇biorxiv.org 14218
Reposted by Zenlabpasteur.bsky.socialAlex Schier @schierlab.bsky.social · 12/03/2026Very happy to see this out. 👏 @yinanwan.bsky.social Bogdan Bintu and team. Whole-embryo spatial transcriptomics at subcellular resolution from gastrulation to organogenesis | free link Science www.science.org/eprint/5MHTM...science.orgWhole-embryo spatial transcriptomics at subcellular resolution from gastrulation to organogenesisGene expression patterns underlie development, but their systematic detection in whole embryos has remained elusive. We introduce a whole-embryo imaging platform using multiplexed error-robust fluores... 618780
Reposted by Zenlabpasteur.bsky.socialTajbakhsh Lab @tajbakhshlab.bsky.social · 21/02/2026Bravo Liza, Brendan and colleagues for the elegant work on multiple live imaging strategies to untangle mechanisms of muscular dystrophy! www.nature.com/articles/s41...nature.comImpaired stem cell migration and divisions in Duchenne muscular dystrophy revealed by live imaging - Nature CommunicationsUsing live-imaging in vivo and ex vivo, Sarde et al. show that dystrophic muscle stem cells have precocious differentiation through symmetric divisions and impaired migration, with differential impact... 12212
Reposted by Zenlabpasteur.bsky.socialParis-Saclay Institute of Neuroscience @neuropsi.bsky.social · 18/01/2026🎖️Toutes nos félicitations à Sylvie Rétaux, cheffe de l'équipe DECA à NeuroPSI, qui a été nommée au grade de Chevalier de la Légion d’Honneur ! 0132
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 04/12/2025The call for abstracts is open to participate in the Ascona conference on "Neurogenesis from development to adulthood in health and disease" ! 000
Reposted by Zenlabpasteur.bsky.socialLabEx Revive @labexrevive.bsky.social · 24/11/2025📣 Register to the 13th Advances in Stem Cell Biology Course #ASCBcourse at the @pasteur.fr @pasteuredu.bsky.social. Hands-on training in different models, embryonic stem cells, iPS cells and much more on #StemCells, #organoids. 🕘June22-July03, 2026 👉 shorturl.at/PyUHi 134
Reposted by Zenlabpasteur.bsky.socialDevelopmental & Stem Cell Biology Pasteur, CNRS UMR3738 @devstempasteur.bsky.social · 19/11/2025Great science & fruiful discussions last Friday the 14th Nov. around "the physics of Stem cells" , the 2025 Stem Cell Initiative & QBio joint symposium. An honour to have Meryem Baghdadi & David Brückner as keynote speakers, who gave inspiring talks. 🙏🙏 to all our speakers & poster presenters. 064
Reposted by Zenlabpasteur.bsky.socialInstitut Pasteur Education @pasteuredu.bsky.social · 19/09/2025⌛1 month left to apply – 2025 PPU call for candidates We invite you to discover 4 Develpmental & Stem Cell Biology projects and 3 Immunology projects👇 🕰️Application deadline: October 20, 2025 📍Program starts: October 2026 Discover all the projects (50+) & apply: www.pasteur.fr/en/education... 045
Reposted by Zenlabpasteur.bsky.socialGaëlle Letort @gaellel.bsky.social · 04/09/2025We (with @nicolasdray.bsky.social @zenlabpasteur.bsky.social) are happy to release officially FishFeats, our #napari plugin to streamline 3D smFish/RNA quantification! 🎊 Available here: github.com/gletort/Fish... user documentation: gletort.github.io/FishFeats/ 14621
Reposted by Zenlabpasteur.bsky.socialDevelopment @dev-journal.bsky.social · 15/08/2025Pathway to Independence – an interview with Joaquín Navajas Acedo. In this interview, @mads100tist.bsky.social talks about about his research vision, zebrafish and the importance of community: journals.biologists.com/dev/article/... 512629
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 05/08/2025New preprint out! Novel transgenic lines directly read out Notch3 signaling in situ, and reveal how adult neural stem cells coordinate quiescence and stemness by integrating spatiotemporal information from Delta and Jagged ligands. Read more: doi.org/10.1101/2025... 052
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 11/07/2025Engineer position, 12 months starting Oct. 1st, 2025, to study the role of NOTCH3 in human iPSC-derived neurogenesis models. Solid previous expertise required in cell culture, notably iPSCs, and organoid generation. Send CV, motivation letter and reference letter(s) to laure.bally-cuif@pasteur.fr 174
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 08/12/2024New call for research Group Leaders at Institut Pasteur Paris ! 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 24/11/2024Just published in Development (@biologists.bsky.social): doi.org/10.1242/dev....doi.orgNon-apoptotic caspase events and Atf3 expression underlie direct neuronal differentiation of adult neural stem cellsHighlighted Article: An inducible Cas3*/Cas7* sensor demonstrates that non-apoptotic caspase events occur in zebrafish neural stem cells. In cooperation with the stress-induced transcription factor At... 22310
Reposted by Zenlabpasteur.bsky.socialDevelopmental & Stem Cell Biology Pasteur, CNRS UMR3738 @devstempasteur.bsky.social · 20/11/2024On Mon 18/11, we held a mini symposium on the novel perspectives in human embryo research @institutpasteur. Lively discussions thanks to our prominent speakers/chair Thanks to Nicolas Rivron; Alain Chédotal; Cécile Martinat; Ken McElreavey; Bernadette De Bakker and the chair Alfonso Martinez Arias 052
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024This also draws yet another parallel between physiological regulation stem cell pools and oncogenesis, which has been referred to as a process through which cells become “asocial” and cease to coordinate with their neighbors (18/18) 030
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024Although we were not able to identify what advantage this confers for NSC homeostasis, this non-canonical maintenance of Notch effectors illustrates different scales of quiescence regulation with some cells seemingly being able to listen less to their neighbors (17/18) 020
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024In vivo experiments confirmed that expression of nr2f1b in zebrafish NSCs is associated with a weaker response to Notch inhibition and functional experiments with morpholinos or electroporations of nr2f1b confirmed its ability to decrease sensitivity to Notch inhibition. (16/18) 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024Interestingly NR2F1 had previously been identified as a regulator of Notch signaling in the mouse inner ear (journals.biologists.com/dev/article/...), with downregulation of Notch effectors in NR2F1 knock-out mutants (15/18) 020
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024Using a gradient boosting method derived from SCENIC applied to both control and treated cells, we identified NR2F1/Coup-TF1 in sillico as a putative regulator enabling this resistance to Notch inhibition. (14/18) 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024Finally, we also identified that one cluster of cells barely responded to Notch inhibition, maintained expression of Notch effectors and did not upregulate genes associated with NSC activation. (13/18) 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024Although there are obvious differences between parenchymal astrocytes in mouse and astrocyte-like RGs in zebrafish, this suggests that comparative studies focusing on these cells might help better understand how to promote brain regeneration. (12/18) 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024The reported properties of the induced activation of these cells indeed shared similarities with that of astrocyte-like NSCs in the zebrafish pallium, such as participation in regeneration or reliance on Notch signaling (11/18) 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024Harmonizing the different reports of cell classification led us to consider that the cells that had been described as latent NSCs under different names in different publications were in fact likely the same population of parenchymal astrocytes close to the lateral ventricles. 10/18 020
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024Using the set of genes which we previously identified as allowing for distinction between murine RGL and astrocytes, we reclassified qNSC1 cells as astrocytes, in accordance with scRNAseq and in situ data from A Cebrian-Silla and methylome data from LPM Kremer. (9/18) 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024We found that the distinction between qNSC1 and qNSC2 had not been made in most cases even when the cells belonging to each group were present in the dataset. (8/18) 020
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024To get a clearer picture we re-analyzed scRNA-seq datasets encompassing the SVZ, generated by the labs of F Doetsch (@doetschlab.bsky.social), A Martin Villalba, J Frisen, S Linnarsson (@slinnarsson.bsky.social), J Biernaskie, N Rajewsky, F Miller, A Alvarez Buylla and O Raineteau. (7/18) 020
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024A similar phenomenon had been described in mouse astrocytes and putative subventricular zone (SVZ) radial glia-like (RGL) (called qNSC1) which respond to injury. (6/18) 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024In particular we found that one group of NSCs, recently described as being very similar to mouse parenchymal astrocytes, is efficiently recruited by Notch inhibition leading to the appearance of a cell state defined by co-expression of astrocytic and activating NSCs markers. (5/18) 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024With scRNAseq after 24hours of Notch inhibition we found that most cells had responded, but not in the same way. (4/18) 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024We attempted to push NSCs out of quiescence using Notch inhibition to see if previously identified clusters reacted differently to a pro-activation stimulus. (3/18) 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024Drawing from previous characterization of the heterogeneity of adult Neural Stem Cells (qNSCs) in the zebrafish pallium and the prediction that some of that heterogeneity comes from differences in quiescence depth (2/18) 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024New pre-print from the lab out on @biorxivpreprint (www.biorxiv.org/content/10.1...). 1/18 030
