Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 04/08/2026Two-year post-doctoral position available in zebrafish developmental neurobiology in the Bally-Cuif and Arimondo labs at Institut Pasteur, Paris, France 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 09/07/2026Heroic work by Tanya Foley to decode the molecular trajectories of individual neural stem cells in situ in the adult brain ! With invaluable help from @cantinilab.bsky.social, @gaellel.bsky.social and @nicolasdray.bsky.social. 040
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 07/07/2026🚨 New pre-print alert! 🚨 Adult neural stem cell state and fate decisions are hidden within transcriptional heterogeneity. To find out how we decoded these heterogeneities to identify a molecularly encoded quiescence cycle, check out the pre-print on bioRxiv! doi: doi.org/10.64898/202... 12815
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 04/12/2025The call for abstracts is open to participate in the Ascona conference on "Neurogenesis from development to adulthood in health and disease" ! 000
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 05/08/2025New preprint out! Novel transgenic lines directly read out Notch3 signaling in situ, and reveal how adult neural stem cells coordinate quiescence and stemness by integrating spatiotemporal information from Delta and Jagged ligands. Read more: doi.org/10.1101/2025... 052
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 11/07/2025Engineer position, 12 months starting Oct. 1st, 2025, to study the role of NOTCH3 in human iPSC-derived neurogenesis models. Solid previous expertise required in cell culture, notably iPSCs, and organoid generation. Send CV, motivation letter and reference letter(s) to laure.bally-cuif@pasteur.fr 174
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 08/12/2024New call for research Group Leaders at Institut Pasteur Paris ! 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024This also draws yet another parallel between physiological regulation stem cell pools and oncogenesis, which has been referred to as a process through which cells become “asocial” and cease to coordinate with their neighbors (18/18) 030
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024Although we were not able to identify what advantage this confers for NSC homeostasis, this non-canonical maintenance of Notch effectors illustrates different scales of quiescence regulation with some cells seemingly being able to listen less to their neighbors (17/18) 020
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024In vivo experiments confirmed that expression of nr2f1b in zebrafish NSCs is associated with a weaker response to Notch inhibition and functional experiments with morpholinos or electroporations of nr2f1b confirmed its ability to decrease sensitivity to Notch inhibition. (16/18) 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024Interestingly NR2F1 had previously been identified as a regulator of Notch signaling in the mouse inner ear (journals.biologists.com/dev/article/...), with downregulation of Notch effectors in NR2F1 knock-out mutants (15/18) 020
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024Using a gradient boosting method derived from SCENIC applied to both control and treated cells, we identified NR2F1/Coup-TF1 in sillico as a putative regulator enabling this resistance to Notch inhibition. (14/18) 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024Finally, we also identified that one cluster of cells barely responded to Notch inhibition, maintained expression of Notch effectors and did not upregulate genes associated with NSC activation. (13/18) 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024Although there are obvious differences between parenchymal astrocytes in mouse and astrocyte-like RGs in zebrafish, this suggests that comparative studies focusing on these cells might help better understand how to promote brain regeneration. (12/18) 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024The reported properties of the induced activation of these cells indeed shared similarities with that of astrocyte-like NSCs in the zebrafish pallium, such as participation in regeneration or reliance on Notch signaling (11/18) 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024Harmonizing the different reports of cell classification led us to consider that the cells that had been described as latent NSCs under different names in different publications were in fact likely the same population of parenchymal astrocytes close to the lateral ventricles. 10/18 020
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024Using the set of genes which we previously identified as allowing for distinction between murine RGL and astrocytes, we reclassified qNSC1 cells as astrocytes, in accordance with scRNAseq and in situ data from A Cebrian-Silla and methylome data from LPM Kremer. (9/18) 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024We found that the distinction between qNSC1 and qNSC2 had not been made in most cases even when the cells belonging to each group were present in the dataset. (8/18) 020
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024To get a clearer picture we re-analyzed scRNA-seq datasets encompassing the SVZ, generated by the labs of F Doetsch (@doetschlab.bsky.social), A Martin Villalba, J Frisen, S Linnarsson (@slinnarsson.bsky.social), J Biernaskie, N Rajewsky, F Miller, A Alvarez Buylla and O Raineteau. (7/18) 020
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024A similar phenomenon had been described in mouse astrocytes and putative subventricular zone (SVZ) radial glia-like (RGL) (called qNSC1) which respond to injury. (6/18) 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024In particular we found that one group of NSCs, recently described as being very similar to mouse parenchymal astrocytes, is efficiently recruited by Notch inhibition leading to the appearance of a cell state defined by co-expression of astrocytic and activating NSCs markers. (5/18) 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024With scRNAseq after 24hours of Notch inhibition we found that most cells had responded, but not in the same way. (4/18) 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024We attempted to push NSCs out of quiescence using Notch inhibition to see if previously identified clusters reacted differently to a pro-activation stimulus. (3/18) 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024Drawing from previous characterization of the heterogeneity of adult Neural Stem Cells (qNSCs) in the zebrafish pallium and the prediction that some of that heterogeneity comes from differences in quiescence depth (2/18) 010
Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · 17/11/2024New pre-print from the lab out on @biorxivpreprint (www.biorxiv.org/content/10.1...). 1/18 030
