Wim Hagen @wimjhh.bsky.social · 29/06/2026On my way to the 2026 Dutch cryo-EM course in Leiden & Delft! 180
Reposted by Wim HagenSimon Fromm @simonfromm.bsky.social · 11/05/2026New preprint🧊🔬Introducing LowDoseWizard—a guided SerialEM workflow for rapid, standardised cryo-TEM setup! Setting up low-dose can be slow & daunting, especially for new users. LowDoseWizard automates optics, camera, and alignment steps from minimal input — full setup in <15 min. #cryoEM #SerialEM 12710
Reposted by Wim HagenGuenter Resch @nexperion.net · 23/09/2025Happy to announce "Technical Innovations Using #SerialEM: A Community Meeting", a new format of hybrid meeting David Mastronarde and I are organizing in April 2026. For more info, a preliminary list of speakers, pre-registration etc. visit www.nexperion.net/semcm2026 05122
Reposted by Wim HagenOli Clarke @olibclarke.bsky.social · 19/09/2025For something different, tried this approach on something on the other end of the spectrum - in situ ribosomes from low SNR 2DTM data. Used the U2OS-grid 9 dataset from EMPIAR-12459 (699 movies; doi.org/10.1016/j.mo...). Blob pick & 2D after denoising gave 12k particles. 2-class HR-HAIR --> 3.3 Å 5349
Reposted by Wim HagenDimitry Tegunov @dtegunov.bsky.social · 01/07/2025Whoever gets the resolution of raw tomograms to <10 Å within the sampled parts of Fourier space would probably deserve a Nobel. Or they'd get one anyway for the crazy new physics they discovered to achieve that. 1234
Reposted by Wim HagenJosh Dickerson @joshdcryoem.bsky.social · 28/06/2025Check out our recent review discussing the merits of tilt series vs single micrographs for in situ cryo-EM! www.sciencedirect.com/science/arti...sciencedirect.comTo tilt or not to tilt? Strategies for in situ cryo-EM data collectionRecent breakthroughs in single-particle cryogenic electron microscopy (cryo-EM) and protein structure prediction have transformed our ability to resol… 16524
Wim Hagen @wimjhh.bsky.social · 21/05/2025Last day of a fantastic Inserm tomography workshop in Bordeaux. 050
Wim Hagen @wimjhh.bsky.social · 22/04/2025Cold showers and warm beer the rest of the week at CCPEM! 030
Wim Hagen @wimjhh.bsky.social · 17/12/2024Ever wondered how some of the automation in cryo-EM data collection works? Read Bram Koster's 1989 thesis! repository.tudelft.nl/record/uuid:...repository.tudelft.nl Autotuning of a transmission electron microscope | TU Delft Repository 0182
Reposted by Wim HagenMisha Kudryashev @mishakudryashev.bsky.social · 13/12/2024Take a look at my personal perspective on the past, present, and future of in situ structural biology with cryo-ET+ While initially skeptical about the genre, I found it fun to write and hopefully, you can enjoy my experiences and some thoughts. Nice weekend y'all www.cambridge.org/core/journal...cambridge.orgThe big chill: Growth of in situ structural biology with cryo-electron tomography | QRB Discovery | Cambridge CoreThe big chill: Growth of in situ structural biology with cryo-electron tomography - Volume 5 45014
Reposted by Wim HagenStructura Biotechnology @structurabio.bsky.social · 09/12/2024Looking for a primer on why the contrast transfer function matters in #cryoEM? Have a look at our updated #CryoSPARC Guide pages on CTF estimation! We cover intuition, theory, job types and practical considerations: guide.cryosparc.com/processing-d... 03417
Wim Hagen @wimjhh.bsky.social · 29/11/2024Phase plates: what do we expect from the next attempt, the laser phase plate? 040
