Reposted by Vincent Burrus 🇨🇦💙💛
Large serine integrases are great genome engineering tools. How do they go downhill both ways? See our set of structures for both the integration and the excision pathway!
authors.elsevier.com/sd/article/S...
Many thanks to collaborator @femijohn.bsky.social and postdoc @heewhanshin.bsky.social
authors.elsevier.com
Structural basis of directionality control in large serine integrases
Large serine integrases catalyze unidirectional insertion of large DNA payloads and, in the presence of a cognate recombination directionality factor …