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femijohn.bsky.social

@femijohn.bsky.social

Reposted by @femijohn.bsky.social

phoeberice.bsky.social @phoeberice.bsky.social · 24/07/2025

Our work expanding the Large Serine Integrase toolkit is finally out! We used alphafold-multimer to identify likely recombination directionality factors and tested them experimentally. Much credit to fantastic postdoc Heewhan Shin. @heewhanshin.bsky.social‬ #Alphafold #MobileGeneticElements

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Reposted by @femijohn.bsky.social

phoeberice.bsky.social @phoeberice.bsky.social · 03/01/2025

Finally posted - our structures that explain directionality control in large serine integrases! Read all about how these amazingly clever molecular swivels are controlled: www.biorxiv.org/content/10.1... Much hard work by postdoc Heewhan Shin and many thanks to collaborator Femi Olorunniji.

biorxiv.org

Structural basis of directionality control in large serine integrases

Large serine integrases (LSIs) catalyze unidirectional site-specific DNA recombination reactions, yet those reactions are reversed by the presence of a cognate recombination directionality factor (RDF...

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