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Reposted by Christian Steinmetzger

Katja Petzold @katjapetzold.bsky.social · 11/05/2026

Imagine if one of RNA’s most common 3D contacts could switch off and on. We identified the A-minor switch that can reversibly “escape” their tertiary contacts, revealing a dynamic regulatory layer in RNA structure (shown by NMR & Cryo-EM and assayed in ribosomes). #RNA #NMR #dynamics #ribosome

biorxiv.org

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Reposted by Christian Steinmetzger

Rubin Dasgupta @rubindg.bsky.social · 07/05/2026

www.nature.com/articles/s41... We detected a third, previously hidden conformation, an intermediate state ES2 in DNA base pair dynamics rewriting a decade old assumption that they are a simple two-state system.

nature.com

1H R1ρ relaxation identifies a hidden intermediate in DNA base-pairing - Nature Communications

Biomolecules function through motion. Enabled by improved applicability of ¹H R1ρ NMR, we identify a hidden DNA intermediate and show how small molecules reshape its structural landscape, highlighting...

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Christian Steinmetzger @stonebutcher.bsky.social · 20/11/2025

With a 250 instead of 200 ms the experiment went to completion. Still curious that it used to work with 200 ms. I also tested the 15N sofast-HMQC library pp sfhmqcf3gpph, with 128 increments, 125 ms D1, 71 ms AQ, and that completed normally. So only the 13C pp sf_mehmqcgpph seems to be affected

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Christian Steinmetzger @stonebutcher.bsky.social · 20/11/2025

Pulse lengths (~8 us 1H 90°) and AQ (50 ms, with 720 points over 12 ppm) and 30 ms disk i/o delay are the same as always. The experiment was already done with digital acquisition mode. Using baseopt also lead to an early abort + the IPSO error below that forced a restart.

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Christian Steinmetzger @stonebutcher.bsky.social · 19/11/2025

For some more details: - TS 3.8.0 - D1 of 200 ms, AQ 50 ms in the direct dimension - indirect TD of 160, typically aborts after 50-70 - With a lower NS, e.g. 8 instead of 720, the experiment goes to completion - A combination of low NS and shorter D1 (e.g. 8 scans with 100 ms) also errors out again

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Christian Steinmetzger @stonebutcher.bsky.social · 19/11/2025

A question for fellow Topspin users: The pp sf_mehmqcgpph from the Bruker standard library has been working well for years, but recently I've been getting this error and the acquisition aborts after only a fraction of increments has been acquired. A console reboot didn't help - any ideas? #nmrchat

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Reposted by Christian Steinmetzger

Rubin Dasgupta @rubindg.bsky.social · 06/10/2025

Check out our latest #NMR study together with @stonebutcher.bsky.social at @katjapetzold.bsky.social lab on an intermediate state of #DNA existing between the well-known Watson–Crick-Franklin and Hoogsteen base pairs stabilised by an anticancer drug Actinomycin D www.biorxiv.org/content/10.1...

biorxiv.org

1H R1ρ Relaxation Identifies a Hidden Intermediate in DNA Base-Pairing

1H R1ρ Relaxation dispersion (RD) NMR experiments provide valuable atomic-level insights into transient, high-energy conformational states of biomolecules. However, cross-relaxation artifacts can hamp...

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Christian Steinmetzger @stonebutcher.bsky.social · 08/09/2025

Check out our new method for targeted DNP-enhanced NMR of RNA using site-directed spin labeling, now live as a preprint on ChemRxiv: doi.org/10.26434/che... A fruitful collaboration with my colleague @rubindg.bsky.social and the group of Prof. Snorri Th. Sigurdsson from the University of Iceland.

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Christian Steinmetzger @stonebutcher.bsky.social · 06/04/2025

If you are there: come see me at poster 110 on Tuesday and Thursday to learn about new developments in nucleic acid DNP measurements

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Christian Steinmetzger @stonebutcher.bsky.social · 06/04/2025

Checked in at Asilomar and looking forward to a week of exciting science at the #ENCISMAR2025 conference in sunny Pacific Grove

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Reposted by Christian Steinmetzger

Gaston Courtade @gcourtade.bsky.social · 04/12/2024

Perspective by Higman et al: deposition of data for titrations, relaxation rates, PREs, etc in a standardized way, e.g. the NEF format could help develop AI models to accurately predict the dynamic behaviour of a protein from the primary sequence alone. #NMRchat doi.org/10.1007/s108...

doi.org

Perspective: on the importance of extensive, high-quality and reliable deposition of biomolecular NMR data in the age of artificial intelligence - Journal of Biomolecular NMR

Artificial intelligence (AI) models are revolutionising scientific data analysis but are reliant on large training data sets. While artificial training data can be used in the context of NMR processin...

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Reposted by Christian Steinmetzger

ENC @enc-conference.org · 04/12/2024

Hi Team #NMRchat #NMR. Happy to see you out here! Let's start spreading the word about the ENC-ISMAR Joint Conference, April 6-10, 2025. Oral Abstract Deadline: January 10 www.enc-conference.org/enc-ismar-2025

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Reposted by Christian Steinmetzger

EHarris @nbgbgb.bsky.social · 01/12/2024

For the season that’s in it an NMR advent calendar for us Chemistry Nerds #Chemistry www.nmr.chemistry.manchester.ac.uk/advent24/

nmr.chemistry.manchester.ac.uk

Advent Calendar

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Reposted by Christian Steinmetzger

Nadia Izadi-Pruneyre @nadia-izadi.bsky.social · 25/11/2024

📣📣 XIVth international conference on #NMR a tool for biology. May 26-28, 2025 @pasteur.fr Paris, France. The registration is open 👉 www.nmr2025.conferences-pasteur.org Coorganized with @brukercorporation.bsky.social

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Christian Steinmetzger @stonebutcher.bsky.social · 23/11/2024

The data browser used to be quite unreliable and slow for me in 4.x (at least on MacOS). TS 4.4.1 has significantly improved on that and now it works well again

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