Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...

Since lipid bilayers are self-assembled macroscopic aggregates, their constituent lipid molecules can spontaneously transition between the two leaflets. This so

dynaMIET enables simultaneous measurement of lateral diffusion and vertical membrane fluctuations in live cells.

Theory and empirical science should be in constant dialogue, but often find it hard to understand one another. Here we describe a graduate-level university course we developed to improve matters. The ...

Phase separation into compositionally and physically distinct domains is ubiquitous in (non)living matter ranging from alloys and emulsions to biomolecular condensates in cells. The organization of th...

Controlling the size of droplets, for example, in biological cells, is challenging because large droplets typically outcompete smaller droplets due to surface tension. This coarsening is generally acc...

Biomolecular condensates govern essential cellular processes yet elude description by traditional equilibrium models. This roadmap, distilled from structured discussions at a workshop and reflecting t...

Biological cells and synthetic analogues use liquid-liquid phase separation to dynamically compartmentalize their environment for various applications. In many cases, multiple droplets need to coexist...

The solubility product is a rigorous description of the phase boundary for salt precipitation and has previously been shown to qualitatively describe the condensation of biomolecules. Here we present a derivation of the solubility product showing that the solubility product is also a robust description of biomolecule phase boundaries if care is taken to account for soluble oligomers and variable composition within the dense phase. Our calculation describes equilibrium between unbound monomers, the dense phase, and an ensemble of oligomer complexes with significant finite-size contributions to their free energy. The biomolecule phase boundary very nearly resembles the power law predicted by the solubility product when plotted as a function of the monomer concentrations. However, this simple form is concealed by the presence of oligomers in the dilute phase. Accounting for the oligomer ensemble introduces complexities to the power law phase boundary including re-entrant behavior and large shifts for stoichiometrically matched molecules. We show that allowing variable stoichiometry in the dense phase expands the two phase region, which appears as curvature of the phase boundary on a double-logarithmic plot. Furthermore, this curvature can be used to predict variations in the dense phase composition at different points along the phase boundary. Finally, we show how the solubility product power law can be identified in experiments by using dilute phase dissociation constants to account for the oligomer ensemble.

Cells operate in part by compartmentalizing chemical reactions. For example, recent work has shown that chromatin, the material that contains the cell's genome, can auto-regulate its structure by util...

Physics of droplet regulation in biological cells, Zwicker, David, Paulin, Oliver W, ter Burg, Cathelijne

Researchers and administrators are exploring ways to restructure a rigid hierarchy that can breed power abuses.

For the independent research group “Theory of Biological Fluids” headed by Dr. David Zwicker we seek to fill a Postdoctoral Position (m/f/d) in Biological Physics.

Nature Chemical Engineering - David Zwicker discusses the delicate balance between entropy and enthalpy that determines whether fluids comprising many components mix or phase separate.

From a 1920s book to nanoscopy – Anthony Hyman, who will deliver the EMBO Lecture at the 49th FEBS Congress in Istanbul in July 2025, shares some of his inspirations and interests.

Tiny specks called biomolecular condensates are leading to a new understanding of the cell

Biomolecular condensates are complex droplets comprising diverse molecules that interact using various mechanisms. Condensation is often driven by short-ranged attraction, but net charges can also med...