De novo protein design often produces thermostable proteins that denature above 100°C, which complicates the analysis of their stability. Thermostable proteins can be unfolded by combined chemical an...

Communications Biology - Clathrin adaptors drive phase separation in endocytosis and trafficking

Liquid-liquid phase separation (LLPS) underlies the formation of biomolecular condensates that organize cellular processes, including endocytosis and membrane trafficking. Although LLPS has been impli...

Circular dichroism (CD) spectroscopy is an established biophysical technique to study chiral molecules. CD allows investigating conformational changes under varying experimental conditions and has bee...

DNA sequencing of the yerba mate (Ilex paraguariensis) genome reveals convergent genomic changes, biochemical pathways, mutations, and substrate-binding orientations responsible for caffeine biosynthe...

The interaction network of Sla2, a vital adaptor protein in the endocytic mid-coat, undergoes constant rearrangement incorporating or replacing interacting proteins over time. Sla2 serves as a scaffol...