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If you’re interested in liver regeneration, cholangiocyte biology, or cell fate plasticity, we’d love to hear your thoughts! #LiverRegeneration #Zebrafish #RegenerativeBiology #CellPlasticity (8/8)

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SinghLab@IRIBHM – Regenerative Biology

🙏 Funded by FNRS and DBTIndia, whose support was essential. 🐟 This study also highlights the power of #zebrafish as a model for dynamic cell plasticity and #regeneration. 🔗 Full paper: nature.com/articles/s41... 🔗 Lab: sumeetpalsingh.github.io (7/8)

sumeetpalsingh.github.io

Injury and Stress Response

Huge thanks to our team: 👏 Lead author Sema Elif Eski 👏 Co-authors @jiaruimi.bsky.social Macarena Pozo Morales, Garnik Hovhannisyan 👏 Collaborators @o-andersson-lab.bsky.social Esteban Gurzov, Rita Manco, Federico Marini (6/8)

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This reveals that cholangiocyte plasticity is not just a backup plan, but an active, physiological response during liver growth and moderate injury. A new paradigm: transdifferentiation can occur without senescence, fibrosis, or chronic damage. (5/8)

Surprisingly, de novo hepatocytes emerged even when spared ones were present—a key deviation from the idea that cholangiocytes only activate after massive hepatocyte loss. We confirmed this with a 17% partial hepatectomy model. ✂️🐟 (4/8)

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Enter CellCousin: a new zebrafish tool we designed to trace liver regeneration. 🟢 Spared hepatocytes 🔴 Ablated hepatocytes 🔵 De novo hepatocytes (not hepatocytes before injury) This lets us precisely map where new hepatocytes come from after injury. (3/8)

The liver can regenerate itself—a fact known since the myth of Prometheus. But when hepatocytes are damaged, can cholangiocytes step in to rebuild the liver even if some hepatocytes remain? We built a new zebrafish model to test it. (2/8)

Filipa Simões @simoesfilipa.bsky.social · 16/05/2025

Reposted by SinghLab@SNIoE

🫀Job alert 🧬 come and be our colleague! Join a BBSRC-funded consortium aiming to understand how the human heart develops - you will be contributing multimodal single cell & spatial transcriptomics data using zebrafish and human cardiac organoids as models. Learn more: www.heartdevelopment.org (1/4)

heartdevelopment.org

CellTalk HHD | Human Heart Development Research

CellTalk-Human Heart Development is a BBSRC funded consortium established to find out more about how cells come together to build the heart as it grows inside the womb.

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SinghLab@SNIoE @singhlab.bsky.social · 02/06/2025

Pleased to have contributed commentary to this recent article by Maria Bolevich for Interesting Engineering, which explores the science of tissue #regeneration and its potential applications in medicine. Read the full article here: interestingengineering.com/health/body-...

interestingengineering.com

Why the body's natural regeneration isn't enough to stop chronic disease

From skin renewal to liver repair, the body is rebuilding itself. Scientists are now uncovering why some organs heal and others don’t.

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Huge thanks to the team and funding support FNRS, DBTIndia that made this happen 🙌 We hope CellCousin2 helps others studying regeneration, cell fate, and tissue repair. All plasmids are available on @addgene.bsky.social

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How does DDCreER work? We fused CreER to a destabilizing domain (DD) that is degraded unless stabilized by TMP. 💊 TMP = protein stabilization 🟣 4-OHT = nuclear translocation Only when both are present does recombination occur—ensuring tight temporal control.

What’s new in CellCousin2? ✅ DDCreER: chemically inducible Cre with low leakiness ✅ NTR2.0: ablation with 10× less metronidazole ✅ Cleaner, more precise regeneration readouts The system is modular and adaptable to other tissues or models.

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SinghLab@SNIoE @singhlab.bsky.social · 26/05/2025

What is CellCousin? It uses stochastic recombination and NTR-based ablation to remove only a subset of hepatocytes. 🟢 Spared cells 🔵 New (De novo) hepatocytes This allows direct comparison of lineage contributions during liver repair.

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Fantastic! Is it available from Etsy or another vendor?

SinghLab@SNIoE @singhlab.bsky.social · 16/05/2025

Stunning 3D fluorescent images of the human #tooth and the first cellular/molecular map of tooth #decay! Hoang Thai Ha from Nicolas Baeyens Lab nails it with tissue clearing + imaging. www.biorxiv.org/content/10.1...

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Reposted by SinghLab@SNIoE

Carol Yang @yhcarolyang.bsky.social · 14/05/2025

Interested in understanding how neural-pancreas interplay contributes to blood sugar control? We have an exciting PhD project to study galanin regulation of pancreatic #islet hormone secretion in live #zebrafish www.exeter.ac.uk/research/ins...

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SinghLab@SNIoE @singhlab.bsky.social · 14/05/2025

Liver regeneration after killing part of the liver! In this image, the same liver from same #zebrafish is imaged before (left) and after (right) regeneration. The newly born liver cells are in blue. Project led by @selifeski.bsky.social

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SinghLab@SNIoE @singhlab.bsky.social · 03/05/2025

We celebrated a big milestone in our lab: our first #Undergrad intern presented her work at lab meeting! She helped develop two new #liver #injury models, and is on her way to becoming a fantastic scientist. Thanks to PhD student Garnik for mentoring her so well! 👏🧪 #TeamScience

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SinghLab@SNIoE @singhlab.bsky.social · 13/04/2025

Notch responsive cells; you have good eyesight!

SinghLab@SNIoE @singhlab.bsky.social · 13/04/2025

The left lobe is better at this stage. Right lobe is also visible, but much smaller :)

SinghLab@SNIoE @singhlab.bsky.social · 12/04/2025

Whole mount EdU staining of 5 day old #zebrafish.

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SinghLab@SNIoE @singhlab.bsky.social · 09/04/2025

Plasmids with fabp10a promoter are on Addgene: www.addgene.org/Sumeet_Pal_S... Unfortunately, we don't use Gateway. Regular cloning with EcoRI/NotI works for these plasmids. Promoter can be removed with SacI/EcoRI.

addgene.org

Addgene: Sumeet Pal Singh Lab Materials

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SinghLab@SNIoE @singhlab.bsky.social · 08/04/2025

I'm excited to share that I will join School of Natural Sciences, Shiv Nadar University as an Associate Professor! Looking forward to working with amazing students and being part of a thriving research community.

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Reposted by SinghLab@SNIoE

Zebrafish Rock! 🎃 @zebrafishrock.bsky.social · 05/03/2025

Come help us build an inclusive community, for those finterested! Did they mention it’s FREE?!? 🧪

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SinghLab@SNIoE @singhlab.bsky.social · 03/03/2025

Delighted to receive FOXO Transcription Factors edited by Dr. Wolfgang Link! FOXO genes are key in stress response & metabolism. Inés Garteizgogeascoa Suñer details a knock-in #zebrafish foxo1a reporter: pubmed.ncbi.nlm.nih.gov/39565585/ Check out the book for key advances!

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SinghLab@SNIoE @singhlab.bsky.social · 14/02/2025

We only do for liver, where cryosections without any detergent work great with Nile Red (yellow), phalloidin (red) and Hoechst. We can share protocol. Works for fish and mouse :)

SinghLab@SNIoE @singhlab.bsky.social · 12/02/2025

Had a great time meeting the students at Freedom Employability Academy (FEA: feaindia.org ) today! Inspiring to see their enthusiasm and growth. Excited to support their journey towards meaningful careers! #Mentorship #Education

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Reposted by SinghLab@SNIoE

Wehner lab | Neuroregeneration @wehnerlab.bsky.social · 27/01/2025

We demonstrate that this biphasic regulation limits the neutrophil-driven acute inflammatory phase, preventing chronic inflammation, fostering a lesion environment with mechano-structural properties conducive to regeneration.

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Reposted by SinghLab@SNIoE

Wehner lab | Neuroregeneration @wehnerlab.bsky.social · 27/01/2025

Fantastic collaboration with the labs of Sven Falk, @singhlab.bsky.social, Jochen Guck, @franzelab.bsky.social, Kanwarpal Singh, and Federico Marini. @fau.de @mpi-scienceoflight.bsky.social Johannes Gutenberg University University of Cambridge @iribhm.bsky.social, @mcmasteruniversity.bsky.social

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Reposted by SinghLab@SNIoE

Valerie Tornini @valetornini.bsky.social · 09/12/2024

We are openly recruiting at all levels at the Tornini Lab at the University of California, Los Angeles (UCLA)! Please share with your networks. For more information, please check the info below, visit www.torninilab.org, or email me directly at vtornini at ucla dot edu. Thank you in advance!

Open recruitment for the Tornini Lab
Department of Integrative Biology and Physiology & Institute for Society and Genetics
Hispanic Serving Institution Infrastructure Initiative
University of California, Los Angeles (UCLA)
www.torninilab.org
We are a new developmental, cell, and molecular biology lab interested in understanding the
genetics of vertebrate neurodevelopment.
We use genome engineering, single-cell technologies, behavioral analyses, pharmacological
profiling, confocal imaging, genomics, and computational biology using the vertebrate
zebrafish (Danio rerio) system and other models (like sea lamprey) for evolutionary studies.
Our work is driven by curiosity, independence and teamwork, fun, a sense of urgency, and a
desire to understand how networks of genes regulate brain and organismal development. This
work can help us better understand basic mechanisms of what happens in human
developmental disorders or diseases and can inform novel therapeutic strategies.
We are recruiting across all levels.
Our lab works in multiple complementary areas (please see below).
We’re looking to match motivated people with what motivates them in a supportive and
engaging environment.
We are recruiting staff research scientists (1-2 years after undergrad+), graduate students (open
rotation positions), postdocs, and other positions as relevant.
While multiple of our projects are already funded, we strongly support candidates to identify
and apply for your own fellowships/grants with the full mentorship and resources of the lab.
Like our research? Think you might be a good fit?
Consider joining us!
For more information, please visit our website, the next page, or contact Val at: vtornini at ucla
dot edu
UCLA is an equal opportunity employer.

Novel micropeptides in vertebrate neurodevelopment
Using zebrafish models, we
ask: what are these proteins; what are their functions in neurodevelopment; what are other
“ignored” or “dark proteome” genes we are missing; and what can they reveal about human
neurodevelopmental disorders or diseases?
Chromatin regulators in vertebrate development and behavior
A growing group of evolutionarily conserved chromatin regulators have been identified as
genes linked with neurodevelopmental disorders, such as autism. In many cases, patients may
struggle with co-occurring conditions that impact daily life in childhood and adulthood,
including seizures, sleeplessness or sleep disruptions, and autoimmune disruptions. How do
these genes work to establish and maintain neural cell types during development, and in turn
shape circuits during early critical periods, including interactions with the environment, to
establish organismal physiological processes?

Non-neuronal regulation during development and aging
Non-neuronal cell types, including oligodendrocytes and microglia, have emerged as key
players in shaping neurodevelopment and into adulthood. How are the cellular, molecular, and
physiological dynamics of these cell types in developing and aging brains affected in mutant
genetic models linked to neurodevelopmental disorders? Can we target these cells to
modulate neural dysregulation and resulting behaviors in these mutant models?

Evolution of vertebrate brain cell states
We seek to understand the principles of how novel genes co-evolve with the emergence of
neural cell types found only in vertebrates.
We use cross-species genomics and functional approaches with the jawless vertebrate model,
sea lamprey, to elucidate the roles of vertebrate-specific genes on the gene regulatory
networks.
Bioethics, neuroethics, and genetics
We seek to integrate evidence-based methods throughout our projects to investigate and address bioethical considerations in this research domain.

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Reposted by SinghLab@SNIoE

Sofia de Oliveira, Ph.D. @sdeoliveira.bsky.social · 08/11/2024

Our debut here 🥰 Non-invasive intravital imaging of wound in skin epithelium of a juvenille zebrafish fed with a High-Cholesterol diet (HCD) versus a Normal Diet (ND) Zebrafish is an outstanding model to study how diet and metabolic disease impact neutrophils Keep following us, spread the word!

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SinghLab@SNIoE @singhlab.bsky.social · 16/01/2025

Amazing data and result! Out of curiosity, does the increase in oxygen also occurs in bones without HSCs; and does this decrease in aging (when HSC function is reduced).

Olov Andersson @o-andersson-lab.bsky.social · 10/01/2025

Reposted by SinghLab@SNIoE

Check out our preprint about liver regeneration, showing hepatic duct heterogeneity and cell plasticity using lineage tracing and single-cell transcriptomics. @zebrafishrock.bsky.social @biorxivpreprint.bsky.social @the-node.bsky.social www.biorxiv.org/content/10.1...

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SinghLab@SNIoE @singhlab.bsky.social · 10/01/2025

And if you’re interested in regenerative biology, visit our lab's website: sumeetpalsingh.github.io 🚀💡(12/12) #LiverRegeneration #Zebrafish

sumeetpalsingh.github.io

SinghLab@IRIBHM – Regenerative Biology

Injury and Stress Response

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SinghLab@SNIoE @singhlab.bsky.social · 10/01/2025

A huge shoutout to the amazing PhD student Sema Elif Eski who lead the project with help from Macarena Pozo-Morales and Gabriel Garnik Hovhannisyan, our amazing team, collaborators Jiarui MI @o-andersson-lab.bsky.social @ritamanco.bsky.social @federicomarini.bsky.social Esteban Gurzov. 🙌 (10/12)