In-cell cryo-electron tomography (cryo-ET) combined with proteomics, integrative structural modeling, and bioinformatics enables discovery and functional annotation of uncharacterized proteins. In the minimal bacterium Mycoplasma pneumoniae, this reveals a complex that links Sec-based protein translocation and extracellular folding, capable of supporting both co- and post-translational pathways.

New research illuminated mechanisms of bacterial protein production, transport, & folding, while better understanding molecular communities.

Cryo-electron tomography (cryo-ET) resolves and quantifies an almost complete view of bacterial translation, revealing how gene expression machineries coordinate across the processes of transcription,...

Cryo-electron tomography has emerged as powerful technology for in-cell structural biology, and in combination with breakthroughs in protein structure prediction, offers a unique opportunity for illum...

Cryo-electron tomography has emerged as powerful technology for in-cell structural biology, and in combination with breakthroughs in protein structure prediction, offers a unique opportunity for illum...

Cryo-electron tomography has emerged as powerful technology for in-cell structural biology, and in combination with breakthroughs in protein structure prediction, offers a unique opportunity for illum...