iiinova — where your digital self keeps evolving

Lennart Hilbert @lennarthilbert.bsky.social · 21h

Haha, the bright future of ubiquitous computing

020

Lennart Hilbert @lennarthilbert.bsky.social · 22h

Should we maybe talk about cars in this context?!

220

Reposted by Lennart Hilbert

Benoit Ladoux @bladoux.bsky.social · 08/10/2026

🔬 We're hiring a Laboratory Manager! Join our growing international team at the Max Planck Center for Physics and Medicine in Erlangen! 🧬 Cell & molecular biology 🧪 Lab coordination & experimental research 📩 Apply now: [www.fau-jobs.de/jobposting/8173a980…]

11517

Lennart Hilbert @lennarthilbert.bsky.social · 07/10/2026

Expansion protocol developed by and learned from @mpownall.bsky.social Label: indirect immunofluorescence against Nup107 Microscope: @zeiss-microscopy.bsky.social LSM 900 Analysis in Python, visualization with @napari.org Code developed w Claude Science, @anthropic.com 10,000 scientist program

030

Lennart Hilbert @lennarthilbert.bsky.social · 07/10/2026

Here another display of the same data, giving a bit more of a quantitative assessment. It is simply a work-in-progress update fresh from the kitchen. I hope you will manage with the German labels of the data. Note the bounding box dimensions: 124.7 × 124.8 × 151.5 µm

120

Lennart Hilbert @lennarthilbert.bsky.social · 07/10/2026

Volumetric display of individual nuclear pore locations and intensities in a single cell nucleus (pluripotent zebrafish embryo). The overall embryo was ~16-fold expanded via ChromExM, the display shows a *single* nucleus, though. Experiments, data, and analysis from our @mofrawe.bsky.social

2227

Lennart Hilbert @lennarthilbert.bsky.social · 02/10/2026

Any questions let me know 👍 Not sure I saw this laser trap probing anywhere before, is it new?

100

Lennart Hilbert @lennarthilbert.bsky.social · 02/10/2026

I digress. Wonderful, clearly written, sense-making paper, pleasure to read. And thanks to @kazu-maeshima.bsky.social for posting the paper, I wouldn’t have spotted it otherwise 🙏

000

Lennart Hilbert @lennarthilbert.bsky.social · 02/10/2026

It’s just so much better usual discourse: every week a new (though same) technique, combative argument, absolutely no sense-making, no attempts to connect diverse facts. Not sure why. Is it egos? Impatience? Poor intellectual culture in (big parts of) biology? It’s hard to take, whatever the cause.

100

Lennart Hilbert @lennarthilbert.bsky.social · 02/10/2026

I looooved this section. It is rare to read “well, all data, techniques, and scales for together, you just have to think a little bit, see: …” Yes, I agree, all scales and structures check out. This analysis supports that view even more!

100

Lennart Hilbert @lennarthilbert.bsky.social · 02/10/2026

Beautiful. Protrusions from the compact domains labeled in black, looks more like computer art than data. Still, works as a data, too - a good visualization is worth a thousand words. This one is great 👍

100

Lennart Hilbert @lennarthilbert.bsky.social · 02/10/2026

Here’s the super comprehensive checking against published ORCA data. Also, amazing these data are accessible for others to use. How it should be, but rarely how it is.

100

Lennart Hilbert @lennarthilbert.bsky.social · 02/10/2026

There’s a lot to like, some highlights. Here, great illustration of the normalization focusing on neighboring nucleoside contact. What a pointed use of simulated data. It becomes just so clear there’s a problem *and* a solution 👌

100

Lennart Hilbert @lennarthilbert.bsky.social · 02/10/2026

Normalization: most important part of any analysis, also the part nobody talks about. This paper, in my view, is all about appropriate normalization. Then check against “all available data”, and get one clean conclusion. Really neat! Joe Paggi @binzmit.bsky.social www.nature.com/articles/s41...

nature.com

Euchromatin forms condensed domains with short active regions on the surface - Nature Genetics

Simulations integrating micro-C and imaging data provide a coherent view of chromatin organization from nucleosomes to clutches to domains, revealing that most regions form compact domains but short r...

172

Lennart Hilbert @lennarthilbert.bsky.social · 02/10/2026

Cool new work from @christoseland.bsky.social lab, looking at interplay of DNA-binding domains and condensation-inducing domains in a huge array estrogen receptor fusion constructs. Good weekend to all :-)

061

Lennart Hilbert @lennarthilbert.bsky.social · 02/10/2026

A lot of cool and intriguing results, as always! The number of constructs and the laser trap data in the last figure … wow 🤩

100

Lennart Hilbert @lennarthilbert.bsky.social · 02/10/2026

And-maybe your system shifts between chromatin-driven condensation (“surface condensation”) and canonical LLPS, depending on how strong the IDR contribution is? We tried to distinguish the two scenarios, see link below. Just skip to Table 3 ;-) nyaspubs.onlinelibrary.wiley.com/share/6B3YHM...

110

Lennart Hilbert @lennarthilbert.bsky.social · 02/10/2026

Also, we studied and thought about chromatin-associated condensates a lot here (“surface condensation”). This review from Sina Wittmann and Thomas Quail is a pretty good overview on the topic, I find. advanced.onlinelibrary.wiley.com/doi/10.1002/...

advanced.onlinelibrary.wiley.com

Current Challenges of Transcription Compartmentalization Research

Transcription factors, coactivators, and RNA polymerase II assemble into transcription compartments ranging from small, defined complexes to liquid-like condensates. This review unifies these seeming...

100

Lennart Hilbert @lennarthilbert.bsky.social · 02/10/2026

It should be possible to get that feature out if the data you already have, if you point your image analysis the right direction? Just a thought :-)

100

Lennart Hilbert @lennarthilbert.bsky.social · 02/10/2026

Hey Chris, congrats and thanks so much for sharing! In the figure with condensates relative to chromatin, I found some interesting details: looks like the condensates tend to have low, but non-zero DNA parts inside. That would fit very much with the picture of anchored condensates? See photo

100

Lennart Hilbert @lennarthilbert.bsky.social · 02/10/2026

Status update from TGV home. Reminds me of student strikes in Germany & Québec. Turning our eyes to the actual issue: Every Euro invested in education & research returns manyfold. Accessible education is an invaluable public good. It is not wise to cut, no matter what decade or where in the world.

000

Lennart Hilbert @lennarthilbert.bsky.social · 02/10/2026

Thanks - the LinkedIn post describes that we already have an excellent colleague working on the laboratory biology / synthetic system construction side. Tandem isn’t intended to describe the hiring process in this case.

000

Lennart Hilbert @lennarthilbert.bsky.social · 02/10/2026

My exclamation is started on capital letter M … been a while I got that smell :-)

010

Lennart Hilbert @lennarthilbert.bsky.social · 02/10/2026

Nothing like a little whiff of tear gas to get you ready for lecture. Campus riots before 9 AM? Vive la France 🥲

110

Lennart Hilbert @lennarthilbert.bsky.social · 01/10/2026

And, now that I know the handle: give a follow to @mar-pan.bsky.social

010

Lennart Hilbert @lennarthilbert.bsky.social · 01/10/2026

I came up with this welcome procedure on a whim in 2018, probably expecting the first papers from a new lab would take quite long, so: celebrate the start, not the end. It’s proven a tested and tried tradition, I counted 26 corks today.

180

Lennart Hilbert @lennarthilbert.bsky.social · 01/10/2026

Welcoming Maria Panarisi into the journey towards the PhD in our group. She looks almost as positively shocked popping the cork as appears to be by this new phase in her scientific life. Looking forward to our joint discoveries in the next years! #journeyintothenucleus

190

Lennart Hilbert @lennarthilbert.bsky.social · 30/09/2026

I wasn’t ready to talk to people and *not* have to explain how surface condensation differs from LLPS, what a pioneer factor is & how that inherently relates to chromatin features. Now somewhat nostalgic for that vibe of a small, well-equipped specialist institute focusing on fundamental biology.

020

Lennart Hilbert @lennarthilbert.bsky.social · 30/09/2026

Great to visit @imbmainz.bsky.social via invite of Sina Wittmann @sfb1551.bsky.social yesterday. My impression was that we are really going after the same questions, Wittmann lab via super strong reconstituted molecular biology, and us top-down by embryo and simulations. Fantastic discussions!

141

Lennart Hilbert @lennarthilbert.bsky.social · 27/09/2026

Hey, totally agree, that’s a statement often presented as an obvious conclusion, but as far as my knowledge goes, condensed==inaccessible is not actually shown to an extent one could call “general”. But Kazuhiro and the Maeshima lab work on this question already much longer than me, so more expert.

020

Lennart Hilbert @lennarthilbert.bsky.social · 27/09/2026

Thank you, too Kazuhiro. I shouldn’t speak too loud as a biologist, but as a physicist, I think: if we hit on a “real” physical mechanism at work in the cell, all data and simulations align. Dispersal by transcription looks suspiciously like such a process. Of course, still many open questions.

010

Reposted by Lennart Hilbert

napari @napari.fosstodon.org.ap.brid.gy · 26/09/2026

🚀 napari is hiring Remote Developers-in-Residence! Join us to build the future of interactive n-dimensional data visualization in Python. 💻 60% Dev / 20% Maintenance / 20% Community & Outreach 💰 $59.50/hr (Contract, 14–35 hrs/wk) 🌍 100% Remote, open globally Learn more and apply today […]

fosstodon.org

Original post on fosstodon.org

0911

Lennart Hilbert @lennarthilbert.bsky.social · 26/09/2026

Thanks for sharing, at first glance from the abstract the concluded model sounds similar also to what we concluded on transcribed euchromatin, getting microdispersed by protruding transcribed regions. Good to see a consensus by these new methods :-) For reference: www.nature.com/articles/s41...

210

Lennart Hilbert @lennarthilbert.bsky.social · 24/09/2026

I hate when this happens. And, let me tell you, it happens. It’s a really bad look … omitting prior, highly relevant work is academically inappropriate, especially if it was brought to one’s attention directly. It violates commonly applicable standards and proper process.

063

Lennart Hilbert @lennarthilbert.bsky.social · 19/09/2026

It’s the boredom resulting from the absence of aggressive, hyper-optimized algorithms that trigger app addiction we have become conditioned for by social media since 20 years.

030

Lennart Hilbert @lennarthilbert.bsky.social · 17/09/2026

This seems like an excellent day to mention: this scientist holds a Canadian PhD and is legally partnered with a Canadian. 🇨🇦🤝🇪🇺

020

Reposted by Lennart Hilbert

Max-Planck-Gesellschaft @maxplanck.de · 09/09/2026

We are hiring! Start at a #MaxPlanckInstitute with your own independent Max Planck Research Group. This position is equivalent to a W2 Professorship at a German university, or Associate Professor internationally. 🔗Apply until October 14th! www.mpg.de/career/max-p... #ScienceCareer #MPRG #TenureTrack

Call for independent Max Planck Research Groups

0112136

Reposted by Lennart Hilbert

Fena Ochs @fenaochs.bsky.social · 10/09/2026

My lab at the Center for Gene Expression (CGEN), University of Copenhagen is looking for a motivated Postdoc interested in cohesin biology, 3D chromatin, and super-resolution microscopy. 📩 Please email your CV and letter of intent to fena.ochs(at)sund.ku.dk.

01822

Lennart Hilbert @lennarthilbert.bsky.social · 09/09/2026

Ah, thanks so much for explaining, it seems the main conclusion is intact then.

030

Lennart Hilbert @lennarthilbert.bsky.social · 08/09/2026

☝️☝️☝️☝️☝️☝️☝️

020

Reposted by Lennart Hilbert

Kazuhiro Maeshima @kazu-maeshima.bsky.social · 08/09/2026

Is euchromatin really “open”? 🧬 Using super-resolution imaging🔬 our new study @natgenet.nature.com reveals: Euchromatin forms condensed domains in live cells. Cohesin constrains them and prevents domain mixing for proper transcriptional insulation🚧 🔗 www.nature.com/articles/s41... (1/2)

415167

Lennart Hilbert @lennarthilbert.bsky.social · 08/09/2026

The preprint already was wonderful, congratulations on the great placement! Is there something new to pay special attention to?

150

Lennart Hilbert @lennarthilbert.bsky.social · 06/09/2026

It didn’t change anything with respect to my unmanageable workload yet, though, so …

020

Lennart Hilbert @lennarthilbert.bsky.social · 05/09/2026

Clearly: commons.wikimedia.org/wiki/File:Tr...

060