5/5
We don’t yet know the mechanism. But the lesson is simple: a fluorescent reporter calibrated in one physiological condition cannot automatically be assumed quantitative in another.
Preprint:
www.biorxiv.org/content/10.6...
biorxiv.org
Fluorescent proteins misreport ribosome abundance under translation inhibition
Fluorescent proteins are widely used as quantitative reporters of protein abundance in living cells. Here we show that this relationship can break down under translation inhibition: the cellular ribos...