Peroxidases have long been considered to use ferryl heme intermediates (Fe(IV) = O) to catalyze oxidative chemistries. Here, Williams et al. find that ferric-oxyl (FeIII–O•–) excited states exist alon...

We characterize the formation of a ferryl state of a dye-decolourizing peroxidase by drop-on-chip serial femtosecond crystallography and also the modulation of the heme redox state using dose-resolved...

Intense, ultrashort X-ray pulses have been used to probe the extraordinary reactions that occur in a light-sensing receptor, resolving a biochemical conundrum.

Communications Chemistry, Published online: 29 January 2026; doi:10.1038/s42004-026-01908-9Time-resolved serial femtosecond crystallography at X-ray free-electron lasers is a powerful approach for studying macromolecular dynamics, but its widespread use is limited by high sample consumption. Here, the authors introduce a segmented-droplet mix-and-inject strategy at the European XFEL that reduces sample consumption by up to 97% while preserving the data quality required for time-resolved structural studies of the enzyme NQO1.

Advanced serial crystallography utilizing high-energy X-rays enhances diffraction efficiency, data quality and structural resolution under room-temperature conditions.

H2 is an ideal energy vector, but catalysts for its clean production from water are inefficient or expensive. [FeFe]-hydrogenases are the most active H2-converting catalysts in nature, using a unique ...

Cellulose, a ubiquitous polysaccharide with critical roles in life, provides structural integrity to cells in plants and aids biofilm formation in many bacteria. Although bacterial cellulose biosynthe...

wwPDB: Worldwide Protein Data Bank

Certain members of the bacterial cytochrome P450 152 family (CYP152) are peroxygenases that catalyse the decarboxylation of fatty acids into terminal …

Certain members of the bacterial cytochrome P450 152 family (CYP152) are peroxygenases that catalyse the decarboxylation of fatty acids into terminal …

Electrostatic potential surfaces and electric field lines reveal that local fields guide proton delivery through multiple conserved solvent channels in heme peroxidases, enabling efficient access to ....

We demonstrate the application of a nitric oxide releasing photocage system for time-resolved serial crystallography studies of two heme containing proteins using a fixed target sample delivery system...

Proteins are constantly moving, but our structures of them are static. Clare Sansom talks to the researchers using free-electron lasers to make time-resolved structures

Genetically modified E coli used to create painkillers from material produced from plastic bottles

Bovine, porcine and human insulin data sets are isomorphous in terms of unit cell and symmetry. Through enhancements to the multi-crystal clustering methods in DIALS, species-pure data sets are automa...

[FeFe] hydrogenases are Nature’s most efficient catalysts for the cleavage and evolution of molecular hydrogen. Despite decades of research, key aspects of the catalytic cycle and the underlying geome...

This training event will explore strategies for producing high-quality, pure, and homogenous recombinant proteins - essential for both structural and functional studies. Aimed at early career research...

Varied pulse-duration and pulse-intensity serial femtosecond crystallography data do not show significant signs of radiation damage under typical experimental conditions.

Streptomyces bacteria make diverse specialised metabolites that form the basis of ~55% of clinically used antibiotics. Despite this, only 3% of their encoded specialised metabolites have been matched ...

This collection reflects research that will advance the understanding of hemoproteins, iron-sulfur cluster proteins and other iron-containing proteins, proteins involved in homeostasis of iron/heme an...

Iron–sulfur clusters are essential protein cofactors synthesized through a complex sequential process. Here the authors show that the assembly of [2Fe–2S] clusters by the iron–sulfur cluster assembly ...

Cytochrome bd from Mycobacterium tuberculosis (Mtbd) is a menaquinol oxidase that has gained interest as an antibiotic target because of its importanc…

Understanding unfolded proteins could boost drug discovery and decipher origins of life mysteries

PhD Project - Design of metallo coiled coil MRI contrast agents at University of Birmingham, listed on FindAPhD.com

ConspectusFuel-forming reactions such as the hydrogen evolution reaction (HER) and CO2 reduction (CO2R) are vital to transitioning to a carbon-neutral economy. The equivalent oxidation reactions are a...