Reposted by Katie Edmonds
Introducing in-cyclo NMR, a breakthrough method led by first author @masgu.bsky.social at @biozentrum.unibas.ch! This method combines high-resolution NMR with ATP regeneration & phosphate removal to resolve kinetics & dynamics of molecular machines - all in one experiment! doi.org/10.1038/s414...
doi.org
Mechanism of ATP hydrolysis in the Hsp70 BiP nucleotide-binding domain - Nature Communications
Mas et al introduce in-cyclo NMR to quantify all states and kinetic steps of Hsp70 chaperones ATPase cycle. In-cyclo NMR will enable studies of other molecular machines at an unprecedented level of de...
